Genomes and Genes
Gene Symbol: RAD9
Species: Saccharomyces cerevisiae S288c
Publications114 found, 100 shown here
- Dpb11 coordinates Mec1 kinase activation with cell cycle-regulated Rad9 recruitmentBoris Pfander
Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire, UK
EMBO J 30:4897-907. 2011..Here, we show that a ternary complex of Dpb11, Mec1 and another key mediator protein Rad9 is required for efficient Rad9 phosphorylation by Mec1 in vitro, and for checkpoint activation in vivo...
- Rif1 supports the function of the CST complex in yeast telomere cappingSavani Anbalagan
Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
PLoS Genet 7:e1002024. 2011..Thus, these data highlight a novel role for Rif1 in assisting the essential telomere protection function of the CST complex...
- Maintenance of the DNA-damage checkpoint requires DNA-damage-induced mediator protein oligomerizationTakehiko Usui
Laboratory of Chromosome Biology, Sloan Kettering Institute, New York, NY 10065, USA
Mol Cell 33:147-59. 2009..cerevisiae BRCT protein Rad9. Our data suggest that Rad9's tandem BRCT domain mediates Rad9 oligomerization via its interaction with its own ..
- Ctf4 coordinates the progression of helicase and DNA polymerase alphaHirokazu Tanaka
Laboratory of Chromosome Structure and Function, Department of Biological Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama City, Kanagawa, Japan
Genes Cells 14:807-20. 2009..These results lead us to propose that Ctf4 is a key connector between DNA helicase and Pol alpha and is required for the coordinated progression of the replisome...
- DNA damage checkpoints are involved in postreplication repairLeslie Barbour
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada
Genetics 174:1789-800. 2006..sublethal dose of a DNA-damaging agent to identify novel genes involved in PRR, which resulted in the isolation of RAD9 as a candidate PRR gene...
- Rad9 BRCT domain interaction with phosphorylated H2AX regulates the G1 checkpoint in budding yeastAndrew Hammet
Wellcome Trust and Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
EMBO Rep 8:851-7. 2007..Furthermore, we show that the tandem BRCT domain of Rad9 interacts directly with phosphorylated H2A in vitro and that a rad9 point mutation that abolishes this interaction ..
- ATRMec1 phosphorylation-independent activation of Chk1 in vivoYinhuai Chen
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0524, USA
J Biol Chem 284:182-90. 2009..Our findings show that a single amino acid substitution in the C terminus, which could lead to an allosteric change in Chk1, allows it to bypass the requirement of the conserved ATR(Mec1) phosphorylation sites for checkpoint function...
- Checkpoint genes and Exo1 regulate nearby inverted repeat fusions that form dicentric chromosomes in Saccharomyces cerevisiaeSalma Kaochar
Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721, USA
Proc Natl Acad Sci U S A 107:21605-10. 2010..inhibit Exo1 in one pathway, whereas in a second pathway the ATR-like kinases Mec1 and Tel1, adaptor protein Rad9, and effector kinases Chk1 and Dun1 act independently of Exo1 to prevent inverted repeat fusion...
- FHA domain-mediated DNA checkpoint regulation of Rad53Marc F Schwartz
Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
Cell Cycle 2:384-96. 2003..Both FHA1 and FHA2 are required for the robust activation of Rad53 by the RAD9-dependent DNA damage checkpoint pathway, while an intact FHA1 or FHA2 allows the activation of Rad53 in response to ..
- Dynamics of Rad9 chromatin binding and checkpoint function are mediated by its dimerization and are cell cycle-regulated by CDK1 activityMagda Granata
Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Milano, Italy
PLoS Genet 6:. 2010Saccharomyces cerevisiae Rad9 is required for an effective DNA damage response throughout the cell cycle...
- Survival and growth of yeast without telomere capping by Cdc13 in the absence of Sgs1, Exo1, and Rad9Hien Ping Ngo
Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, United Kingdom
PLoS Genet 6:e1001072. 2010..We found that simultaneous inactivation of Sgs1, Exo1, and Rad9, three DNA damage response (DDR) proteins, is sufficient to allow cell division in the absence of Cdc13...
- Choreography of the DNA damage response: spatiotemporal relationships among checkpoint and repair proteinsMichael Lisby
Department of Genetics and Development, Columbia University, College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032, USA
Cell 118:699-713. 2004....
- RAD53, DUN1 and PDS1 define two parallel G2/M checkpoint pathways in budding yeastR Gardner
Department of Molecular and Cellular Biology, The University of Arizona, PO Box 21016, Tucson, AZ 85721 0106, USA
EMBO J 18:3173-85. 1999..A current model posits three gene classes: those encoding proteins acting on damaged DNA (e.g. RAD9 and RAD24), those transducing a signal (MEC1, RAD53 and DUN1) or those participating more directly in arrest (PDS1)...
- The Saccharomyces cerevisiae DNA damage checkpoint is required for efficient repair of double strand breaks by non-homologous end joiningM de la Torre-Ruiz
Imperial Cancer Research Fund, Clare Hall Laboratories, CDC Laboratory, South Mimms, UK
FEBS Lett 467:311-5. 2000In this work we report that the Saccharomyces cerevisiae RAD9, RAD24, RAD17, MEC1, MEC3 and RAD53 checkpoint genes are required for efficient non-homologous end joining (NHEJ). RAD9 and RAD24 function additionally in this process...
- Dissection of Rad9 BRCT domain function in the mitotic checkpoint response to telomere uncappingCHINONYE C NNAKWE
Department of Pathology, The University of Chicago, Chicago, IL 60637, USA
DNA Repair (Amst) 8:1452-61. 2009..Throughout the cell cycle, the multi-domain adaptor protein Rad9 is required for the activation of checkpoint effector kinase Rad53 in response to DSBs and is similarly necessary ..
- Mrc1 is a replication fork component whose phosphorylation in response to DNA replication stress activates Rad53Alexander J Osborn
Verna and Marrs MacLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
Genes Dev 17:1755-67. 2003....
- The Saccharomyces recombination protein Tid1p is required for adaptation from G2/M arrest induced by a double-strand breakS E Lee
Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454, USA
Curr Biol 11:1053-7. 2001..mutations yku70Delta and cdc5-ad, permanent arrest in tid1Delta is bypassed by the deletion of the checkpoint gene RAD9. Permanent arrest of tid1Delta cells is suppressed by the rfa1-t11 mutation in the ssDNA binding complex RPA, ..
- Genetic and physical interactions between DPB11 and DDC1 in the yeast DNA damage response pathwayHong Wang
Verna and Marrs McLean Department of Biochemistry and Molecular Biology and Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
Genetics 160:1295-304. 2002..These results suggest that DPB11 and DDC1 may function in the same or parallel pathways after DNA damage and that DDC1 may play a role in responding to replication defects...
- The novel DNA damage checkpoint protein ddc1p is phosphorylated periodically during the cell cycle and in response to DNA damage in budding yeastM P Longhese
Dipartimento di Genetica e di Biologia dei Microrganismi, Universita degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy
EMBO J 16:5216-26. 1997..Moreover, Ddc1p is phosphorylated periodically during a normal cell cycle and becomes hyperphosphorylated in response to DNA damage. Both phosphorylation events are at least partially dependent on a functional MEC3 gene...
- RAD9 and RAD24 define two additive, interacting branches of the DNA damage checkpoint pathway in budding yeast normally required for Rad53 modification and activationM A de la Torre-Ruiz
Imperial Cancer Research Fund, Clare Hall Laboratories, CDC Laboratory, South Mimms, Herts EN6 3LD, UK
EMBO J 17:2687-98. 1998In budding yeast, RAD9 and RAD24/RAD17/MEC3 are believed to function upstream of MEC1 and RAD53 in signalling the presence of DNA damage...
- Cloning and sequence analysis of the Saccharomyces cerevisiae RAD9 gene and further evidence that its product is required for cell cycle arrest induced by DNA damageR H Schiestl
Department of Biology, University of Rochester, River Campus Station, New York 14627
Mol Cell Biol 9:1882-96. 1989..Here, we provide evidence that the Saccharomyces cerevisiae RAD9 gene, mutations of which confer sensitivity to DNA-damaging agents, is necessary for the cell cycle arrest ..
- Yeast checkpoint genes in DNA damage processing: implications for repair and arrestD Lydall
Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721, USA
Science 270:1488-91. 1995..Another checkpoint gene, RAD9, had a different role: It inhibited the degradation by RAD17, RAD24, and MEC3...
- Saccharomyces Ku70, mre11/rad50 and RPA proteins regulate adaptation to G2/M arrest after DNA damageS E Lee
Rosenstiel Center, Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
Cell 94:399-409. 1998..hdf1 cells, lacking Ku70p, fail to escape from this RAD9/RAD17-dependent checkpoint...
- The yeast Shu complex couples error-free post-replication repair to homologous recombinationLindsay G Ball
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E5
Mol Microbiol 73:89-102. 2009..This mechanism appears to be conserved throughout eukaryotes...
- Loss of Sin3/Rpd3 histone deacetylase restores the DNA damage response in checkpoint-deficient strains of Saccharomyces cerevisiaeKenneth L Scott
Department of Molecular and Human Genetics, Texas Children s Cancer Center, Baylor College of Medicine, Houston, Texas 77030, USA
Mol Cell Biol 23:4522-31. 2003..Deletion of either SIN3 or RPD3 in rad9 or mec1 checkpoint mutant strains suppresses sensitivity to replication blocks and DNA damage resulting from Cdc9 ..
- PP2C phosphatases Ptc2 and Ptc3 are required for DNA checkpoint inactivation after a double-strand breakChristophe Leroy
Service de Biochimie et de Génétique Moléculaire, CEA Saclay, 91191 Gif sur Yvette, Cedex, France
Mol Cell 11:827-35. 2003..In vivo and in vitro evidence suggests that phosphorylated forms of Ptc2 and Ptc3 specifically bind to the Rad53 FHA1 domain and inactivate Rad53-dependent pathways during adaptation and recovery by dephosphorylating Rad53...
- EXO1-dependent single-stranded DNA at telomeres activates subsets of DNA damage and spindle checkpoint pathways in budding yeast yku70Delta mutantsLaura Maringele
School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK
Genes Dev 16:1919-33. 2002..We show that CHK1, MEC1, and RAD9 checkpoint genes are required for efficient cell cycle arrest of yku70Delta mutants cultured at 37 degrees C, ..
- MRX protects telomeric DNA at uncapped telomeres of budding yeast cdc13-1 mutantsSteven S Foster
Institute for Ageing and Health, University of Newcastle, Henry Wellcome Laboratory for Biogerontology Research, Newcastle General Hospital, Newcastle upon Tyne NE4 6BE, UK
DNA Repair (Amst) 5:840-51. 2006..Instead, we find that Rad50 inhibits ssDNA accumulation and promotes cdc13-1 cell viability, consistent with a major role for MRX in telomere capping...
- Mdt1, a novel Rad53 FHA1 domain-interacting protein, modulates DNA damage tolerance and G(2)/M cell cycle progression in Saccharomyces cerevisiaeBrietta L Pike
St Vincent s Institute of Medical Research, Department of Medicine, St Vincent s Hospital, The University of Melbourne, Fitzroy, Victoria 3065, Australia
Mol Cell Biol 24:2779-88. 2004..The data indicate that Mdt1 is involved in normal G(2)/M cell cycle progression and is a novel target of checkpoint-dependent cell cycle arrest pathways...
- Exo1 and Rad24 differentially regulate generation of ssDNA at telomeres of Saccharomyces cerevisiae cdc13-1 mutantsMikhajlo K Zubko
School of Clinical Medical Sciences Gerontology, University of Newcastle, Henry Wellcome Laboratory for Biogerontology Research, Newcastle upon Tyne, NE4 6BE, UK
Genetics 168:103-15. 2004..We show that Exo1 is unique among the repair genes tested because like Rad9 and Rad24 checkpoint proteins, Exo1 inhibits the growth of cdc13-1 mutants at the semipermissive temperatures...
- Saccharomyces cerevisiae Rad9 acts as a Mec1 adaptor to allow Rad53 activationFrederic D Sweeney
Centre for Systems Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada
Curr Biol 15:1364-75. 2005..Despite having identified many components of this signaling cascade, the exact mechanisms by which checkpoint kinases are activated after DNA damage, as well as the role of the checkpoint mediators, remain poorly understood...
- DNA degradation at unprotected telomeres in yeast is regulated by the CDK1 (Cdc28/Clb) cell-cycle kinaseMomchil D Vodenicharov
Department of Microbiology, Faculty of Medicine, Universite de Sherbrooke, 3001 12e Ave Nord, Sherbrooke, Quebec J1H 5N4, Canada
Mol Cell 24:127-37. 2006..These results strongly suggest that after a loss of the telomere capping function, telomere-led genome instability is caused by tightly regulated cellular DNA repair attempts...
- Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9Robert Wysocki
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA
Mol Cell Biol 25:8430-43. 2005..Consistent with this paradigm, loss of Dot1 prevents activation of the yeast 53BP1 ortholog Rad9 or Chk2 homolog Rad53 and decreases binding of Rad9 to DSBs after DNA damage...
- The budding yeast Rad9 checkpoint protein is subjected to Mec1/Tel1-dependent hyperphosphorylation and interacts with Rad53 after DNA damageJ E Vialard
Imperial Cancer Research Fund, Clare Hall Laboratories, CDC Laboratory, South Mimms, Hertfordshire EN6 3LD, UK
EMBO J 17:5679-88. 1998The Saccharomyces cerevisiae RAD9 checkpoint gene is required for transient cell-cycle arrests and transcriptional induction of DNA repair genes in response to DNA damage...
- Rad53 FHA domain associated with phosphorylated Rad9 in the DNA damage checkpointZ Sun
Department of Biology, Yale University, New Haven, CT 06511, USA
Science 281:272-4. 1998..The Rad9 protein was phosphorylated in response to DNA damage, and phosphorylated Rad9 interacted with the COOH-terminal ..
- The Saccharomyces cerevisiae RAD9, RAD17, RAD24 and MEC3 genes are required for tolerating irreparable, ultraviolet-induced DNA damageA G Paulovich
Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Genetics 150:75-93. 1998..We find that RAD9, RAD17, RAD24, and MEC3 are required for UV-induced (although not spontaneous) mutagenesis, and that RAD9 and RAD17 ..
- Single-stranded DNA arising at telomeres in cdc13 mutants may constitute a specific signal for the RAD9 checkpointB Garvik
Department of Genetics, University of Washington, Seattle 98195, USA
Mol Cell Biol 15:6128-38. 1995..in the G2 phase of the cell cycle at the restrictive temperature as a result of DNA damage that activates the RAD9 checkpoint...
- Budding yeast Rad9 is an ATP-dependent Rad53 activating machineC S Gilbert
Imperial Cancer Research Fund, Clare Hall Laboratories, CDC Laboratory, South Mimms, EN6 3LD, Hertfordshire, United Kingdom
Mol Cell 8:129-36. 2001We find budding yeast Rad9 in two distinct, large, and soluble complexes in cell extracts...
- Cdc13 prevents telomere uncapping and Rad50-dependent homologous recombinationN Grandin
UMR CNRS ENS no 5665, Ecole Normale Superieure de Lyon, 46, Allee d Italie, 69364 Lyon, France
EMBO J 20:6127-39. 2001..We propose that Cdc13 prevents telomere uncapping and inhibits recombination between telomeric sequences through a pathway distinct from and complementary to that used by telomerase...
- The BRCT domain of the S. cerevisiae checkpoint protein Rad9 mediates a Rad9-Rad9 interaction after DNA damageJ Soulier
Imperial Cancer Research Fund, Clare Hall Laboratories, CDC Laboratory, South Mimms, Hertfordshire, EN6 3LD, UK
Curr Biol 9:551-4. 1999The Saccharomyces cerevisiae checkpoint protein Rad9 is required for transient cell-cycle arrest and transcriptional induction of DNA-repair genes in response to DNA damage ...
- MEC1-dependent phosphorylation of Rad9p in response to DNA damageA Emili
Division of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Mol Cell 2:183-9. 1998In budding yeast, DNA damage can activate a checkpoint surveillance system controlled by the RAD9, RAD53, and MEC1 genes, resulting in a delay in cell cycle progression...
- Histone methyltransferase Dot1 and Rad9 inhibit single-stranded DNA accumulation at DSBs and uncapped telomeresFederico Lazzaro
Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Milano, Italy
EMBO J 27:1502-12. 2008..Here, we provide evidence that binding of the checkpoint protein Rad9, through its Tudor domain, to methylated histone H3-K79 inhibits resection at DSBs and uncapped telomeres...
- Histone H2A phosphorylation and H3 methylation are required for a novel Rad9 DSB repair function following checkpoint activationGeraldine W L Toh
Genome Stability Laboratory, Department of Biochemistry and National Centre for Biomedical Engineering Science, National University of Ireland, University Road, Galway, Ireland
DNA Repair (Amst) 5:693-703. 2006In budding yeast, the Rad9 protein is an important player in the maintenance of genomic integrity and has a well-characterised role in DNA damage checkpoint activation...
- The role of Stn1p in Saccharomyces cerevisiae telomere capping can be separated from its interaction with Cdc13pRuben C Petreaca
Cell, Molecular, and Developmental Biology Graduate Program, Department of Cell Biology and Neuroscience, University of California, Riverside, California 92506, USA
Genetics 177:1459-74. 2007..Thus, an amino-terminal region of Stn1p is sufficient for its essential function, while a central region of Stn1p either negatively regulates the STN1 essential function or destabilizes the mutant Stn1 protein...
- A DNA damage response pathway controlled by Tel1 and the Mre11 complexT Usui
Department of Biology, Graduate School of Science, Osaka University, Toyonaka, 560 0043, Osaka, Japan
Mol Cell 7:1255-66. 2001..In mitotic cells, the Tel1-Mre11 complex pathway triggers Rad53 activation and its interaction with Rad9, whereas in meiosis it acts via Rad9 and the Rad53 paralog Mre4/Mek1...
- Replication protein A-mediated recruitment and activation of Rad17 complexesLee Zou
Brigham and Women s Hospital, Howard Hughes Medical Institute, Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 100:13827-32. 2003The human Rad17-Rfc2-5 and Rad9-Rad1-Hus1 complexes play crucial roles in the activation of the ATR-mediated DNA damage and DNA replication stress response pathways...
- Rad9 phosphorylation sites couple Rad53 to the Saccharomyces cerevisiae DNA damage checkpointMarc F Schwartz
Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA
Mol Cell 9:1055-65. 2002b>Rad9 is required for the MEC1/TEL1-dependent activation of Saccharomyces cerevisiae DNA damage checkpoint pathways mediated by Rad53 and Chk1...
- Analysis of replication profiles reveals key role of RFC-Ctf18 in yeast replication stress responseLaure Crabbe
Institute of Human Genetics, Centre National de Recherche Scientifique, Unité Propre de Recherche 1142, Montpellier, France
Nat Struct Mol Biol 17:1391-7. 2010..These data identify RFC(Ctf18) as a key DRC mediator, potentially bridging Mrc1 and primed ssDNA to signal paused forks...
- A domain of Rad9 specifically required for activation of Chk1 in budding yeastRichard T Blankley
School of Biological Sciences, University of Manchester, G38 Stopford Building, Oxford Rd, Manchester, M13 9PT, UK
J Cell Sci 117:601-8. 2004The Rad9 protein is a key adaptor protein in Saccharomyces cerevisiae DNA damage checkpoint pathways...
- Yeast pip3/mec3 mutants fail to delay entry into S phase and to slow DNA replication in response to DNA damage, and they define a functional link between Mec3 and DNA primaseM P Longhese
Dipartimento di Genetica e di Biologia dei Micorganismi, Universita degli Studi di Milano, Italy
Mol Cell Biol 16:3235-44. 1996....
- The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNANathalie Lancelot
Institut de Biologie et Technologies de Saclay, CEA Saclay, 91191 Gif sur Yvette, France
Nucleic Acids Res 35:5898-912. 2007..Budding yeast Rad9, fission yeast Crb2 and metazoan 53BP1 are presented as mediators involved in the activation of checkpoint kinases...
- The SUMO isopeptidase Ulp2p is required to prevent recombination-induced chromosome segregation lethality following DNA replication stressMing Ta Lee
Department of Cell Biology and Neuroscience, University of California Riverside, Riverside, California, United States of America
PLoS Genet 7:e1001355. 2011..At the same time, Ulp2p is also required to either suppress or resolve recombination-induced attachments between sister chromatids. These opposing defects may synergize to greatly increase the toxicity of DNA replication stress...
- Quantitative proteomic analysis of chromatin reveals that Ctf18 acts in the DNA replication checkpointTakashi Kubota
Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK
Mol Cell Proteomics 10:M110.005561. 2011..Identification of Ctf18 as a checkpoint protein highlights the usefulness of chromatin proteomic analysis for understanding the in vivo function of proteins that mediate chromatin transactions...
- Pph3-Psy2 is a phosphatase complex required for Rad53 dephosphorylation and replication fork restart during recovery from DNA damageBryan M O'Neill
Departments of Chemistry and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 104:9290-5. 2007..These findings suggest that Rad53 regulates replication fork restart and initiation of late firing origins independently and that regulation of these processes is mediated by specific Rad53 phosphatases...
- Proteins in the nutrient-sensing and DNA damage checkpoint pathways cooperate to restrain mitotic progression following DNA damageJennifer S Searle
Department of Pharmacology and Toxicology, Norris Cotton Cancer Center, Dartmouth Medical School, Hanover, New Hampshire, USA
PLoS Genet 7:e1002176. 2011..Our data indicate that there is cross-talk between the DNA damage checkpoint and the proteins that integrate nutrient and stress signals to regulate PKA...
- [Interaction between checkpoint genes RAD9, RAD17, RAD24, and RAD53 involved in the determination of yeast Saccharomyces cerevisiae sensitivity to ionizing radiation]N A Koltovaia
Genetika 44:761-70. 2008..To clarify the role of checkpoint genes RAD9, RAD17, RAD24, and RAD53 in cell radioresistance, double mutants were analyzed for cell sensitivity to ionizing ..
- Orchestration of the S-phase and DNA damage checkpoint pathways by replication forks from early originsJulie M Caldwell
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
J Cell Biol 180:1073-86. 2008..Thus, oncogene-mediated deregulation of cyclins in the early stages of cancer development could contribute to genomic instability through a deficiency in the forks required to establish the S-phase checkpoint...
- PP2A (Cdc)⁵⁵ is required for multiple events during meiosis IJocelyn K Nolt
Venenum Biodesign, Genesis Biotechnology Group, Hamilton, NJ, USA
Cell Cycle 10:1420-34. 2011..Finally, the premeiotic replication defect is suppressed by loss of Rad9 checkpoint function...
- The role of replication bypass pathways in dicentric chromosome formation in budding yeastAndrew L Paek
Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USA
Genetics 186:1161-73. 2010..Fourth, by studying genes implicated in suppression of GCRs in other studies, we found that inverted repeat fusion has a profile of genetic regulation distinct from these other major forms of GCR formation...
- RAD9, RAD17, and RAD24 are required for S phase regulation in Saccharomyces cerevisiae in response to DNA damageA G Paulovich
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA
Genetics 145:45-62. 1997..In this report, we show that other genes (RAD9, RAD17, RAD24) involved in the DNA damage checkpoint pathway also play a role in regulating S phase in response to ..
- Genome-wide analysis of factors affecting transcription elongation and DNA repair: a new role for PAF and Ccr4-not in transcription-coupled repairHélène Gaillard
Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Sevilla, Spain
PLoS Genet 5:e1000364. 2009....
- Contrasting roles of checkpoint proteins as recombination modulators at Fob1-Ter complexes with or without fork arrestBidyut K Mohanty
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
Eukaryot Cell 8:487-95. 2009..Other checkpoint proteins of the checkpoint adapter/mediator class such as Mrc1 and Rad9, which channel signals from the sensor to the effector kinase, tended to suppress recombination at Fob1-Ter ..
- Post-replication repair suppresses duplication-mediated genome instabilityChristopher D Putnam
Ludwig Institute for Cancer Research, University of California San Diego School of Medicine, La Jolla, California, United States of America
PLoS Genet 6:e1000933. 2010..Our analysis is consistent with models in which PRR prevents replication damage from becoming double strand breaks (DSBs) and/or regulates the activity of HR on DSBs...
- Characterization of G1 checkpoint control in the yeast Saccharomyces cerevisiae following exposure to DNA-damaging agentsW Siede
Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235
Genetics 138:271-81. 1994..treatment of yeast cells with DNA-damaging agents is an actively regulated response that requires functional RAD9 and RAD24 genes...
- Sua5p a single-stranded telomeric DNA-binding protein facilitates telomere replicationFei Long Meng
The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, China
EMBO J 28:1466-78. 2009..Thus, Sua5p represents a novel ssTG DNA-binding protein and positively regulates the telomere length in vivo...
- Functional connection between the Clb5 cyclin, the protein kinase C pathway and the Swi4 transcription factor in Saccharomyces cerevisiaeEthel Queralt
Departament de Bioquimica i Biologia Molecular, Universitat de Valencia, C Dr Moliner 50, E 46100 Burjassot, Valencia, Spain
Genetics 171:1485-98. 2005..and Swi4 are involved in the control of DNA integrity: there is a synthetic interaction between pkc1 and slt2 with rad9; the pkc1, slt2, and swi4 mutants are hypersensitive to hydroxyurea; and the Slt2 kinase is activated by ..
- Previously uncharacterized genes in the UV- and MMS-induced DNA damage response in yeastDenise Hanway
Department of Chemistry and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 99:10605-10. 2002..Epistatsis analysis of four of the genes was performed to determine the DNA damage repair pathways in which the protein products function...
- Solution structures of two FHA1-phosphothreonine peptide complexes provide insight into the structural basis of the ligand specificity of FHA1 from yeast Rad53C Yuan
Department of Chemistry, The Ohio State University, Columbus OH 43210, USA
J Mol Biol 314:563-75. 2001..Subsequent location of this motif within the sequence of Rad9, the target protein, coupled with spectroscopic analysis has led to identification of a tight binding sequence that ..
- A dominant-negative MEC3 mutant uncovers new functions for the Rad17 complex and Tel1Michele Giannattasio
Dipartimento di Genetica e Biologia dei Microrganismi, Universita degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy
Proc Natl Acad Sci U S A 99:12997-3002. 2002..We also show that the function of the G(2) checkpoint in mutant cells is maintained by an uncharacterized activity of Tel1, the yeast homologue of ATM. This work thus reports a previously undiscovered role for Tel1 in checkpoint control...
- A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulatorMichael Downey
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON, M5G 1X5, Canada
Cell 124:1155-68. 2006..Our results therefore indicate that the KEOPS complex promotes both telomere uncapping and telomere elongation...
- Suppression of genome instability by redundant S-phase checkpoint pathways in Saccharomyces cerevisiaeKyungjae Myung
Ludwig Institute for Cancer Research, Cancer Center, and Department of Medicine, University of California at San Diego School of Medicine, La Jolla, CA 92093, USA
Proc Natl Acad Sci U S A 99:4500-7. 2002..These data support the view that spontaneous genome rearrangements result from DNA replication errors and indicate that there is a high degree of redundancy among the checkpoints that act in S phase to suppress such genome instability...
- Saccharomyces cerevisiae chromatin-assembly factors that act during DNA replication function in the maintenance of genome stabilityKyungjae Myung
Ludwig Institute for Cancer Research, Cancer Center and Department of Medicine, University of California at San Diego School of Medicine, La Jolla 92093, USA
Proc Natl Acad Sci U S A 100:6640-5. 2003..These results indicate that coupling of chromatin assembly to DNA replication and DNA repair is critical to maintaining genome stability...
- In vivo analysis reveals that the interdomain region of the yeast proliferating cell nuclear antigen is important for DNA replication and DNA repairN S Amin
Department of Pharmacology, University of California at San Diego, La Jolla 92093 0651, USA
Genetics 144:479-93. 1996..At the restrictive temperature, the Cs- pol30 mutants undergo a RAD9-dependent arrest as large-budded cells with a 2c DNA content...
- A novel role for the budding yeast RAD9 checkpoint gene in DNA damage-dependent transcriptionA Aboussekhra
Institut Curie, UMR 144 CNRS, 26 Rue d Ulm, 75231 Paris Cedex 05, France
EMBO J 15:3912-22. 1996..The G1 and G2 checkpoints have been shown previously to be under the control of the RAD9 gene. We show here that RAD9 is also required for the transcriptional response to DNA damage...
- The RAD9-dependent gene trans-activation is required for excision repair of active genes but not for repair of non-transcribed DNANisreen M Al-Moghrabi
King Faisal Specialist Hospital and Research Center, Department of Biological and Medical Research, Riyadh, Saudi Arabia
Mutat Res 663:60-8. 2009The Saccharomyces cerevisiae RAD9 and RAD24 are two cell cycle checkpoint genes required for UV-dependent up-regulation of a battery of genes involved in different metabolic pathways...
- RAD9, RAD24, RAD16 and RAD26 are required for the inducible nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers from the transcribed and non-transcribed regions of the Saccharomyces cerevisiae MFA2 geneS Yu
School of Biological Sciences, University of Wales Swansea, Singleton Park, SA2 8PP, Swansea, UK
Mutat Res 485:229-36. 2001..No inducible repair was observed in rad9, rad24, rad16 and rad26 cells, indicating two checkpoint genes RAD9 and RAD24, the global repair gene RAD16 and the ..
- Association of Rad9 with double-strand breaks through a Mec1-dependent mechanismTakahiro Naiki
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 0814, Japan
Mol Cell Biol 24:3277-85. 2004b>Rad9 is required for the activation of DNA damage checkpoint pathways in budding yeast. Rad9 is phosphorylated after DNA damage in a Mec1- and Tel1-dependent manner and subsequently interacts with Rad53...
- Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replicationTakashi Ohuchi
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
DNA Repair (Amst) 8:690-6. 2009..Double mutation of RAD51 and RAD53 exhibited the similar levels of Rad52 sumoylation to RAD53 single mutation. The significance and regulation mechanism of Rad52 sumoylation by checkpoint pathways will be discussed...
- Yeast Rad52 and Rad51 recombination proteins define a second pathway of DNA damage assessment in response to a single double-strand breakSang Eun Lee
Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
Mol Cell Biol 23:8913-23. 2003..We suggest that monitoring of the extent of DNA damage depends on independent binding of RPA and Rad52p to ssDNA, with Rad52p's activity modulated by Rad51p whereas RPA's action depends on Tid1p...
- CDC5 inhibits the hyperphosphorylation of the checkpoint kinase Rad53, leading to checkpoint adaptationGenevieve M Vidanes
Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, USA
PLoS Biol 8:e1000286. 2010..Mec1 appeared to be active, since the Rad9 adaptor retained its Mec1 phosphorylation...
- Mitotic catastrophe induced by overexpression of budding yeast Rad2pMi Sun Kang
Department of Pharmacology, Inha Research Institute for Medical Sciences, Incheon 400 712, Republic of Korea
Yeast 27:399-411. 2010..However, Rad2p-induced cell growth arrest is released by rad9 deletion but is not affected by downstream DNA damage checkpoint genes...
- A postincision-deficient TFIIH causes replication fork breakage and uncovers alternative Rad51- or Pol32-mediated restart mechanismsMaría Moriel-Carretero
Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Av Americo Vespucio s n, 41092 Sevilla, Spain
Mol Cell 37:690-701. 2010..Our results define the genetic and molecular hallmarks of replication fork breakage and restart and bring insights to understand specific NER-related human syndromes...
- Rpb1 sumoylation in response to UV radiation or transcriptional impairment in yeastXuefeng Chen
Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, United States of America
PLoS ONE 4:e5267. 2009..Our results demonstrate a novel covalent modification of Rpb1 in response to UV induced DNA damage or transcriptional impairment, and unravel an important link between the modification and the DNA damage checkpoint response...
- HYS2, an essential gene required for DNA replication in Saccharomyces cerevisiaeK Sugimoto
Department of Molecular Biology, Faculty of Science, Nagoya University, Japan
Nucleic Acids Res 23:3493-500. 1995..Finally, deletion of RAD9 in a hys2-1 strain decreases the percentage of arrested cells, suggesting that an intact RAD9-checkpoint is ..
- Pathways for repair of topoisomerase I covalent complexes in Saccharomyces cerevisiaeJ J Pouliot
Laboratory of Molecular Biology, National Institute of Mental Health, Building 36, Room 1B08, Bethesda, MD 20892 4034, USA
Genes Cells 6:677-87. 2001..Tdp1, an enzyme that can hydrolyse the bond between topoisomerase I and DNA, is thought to be involved in the repair of these lesions, but little is known about how such repair is accomplished...
- Nuclear oscillations and nuclear filament formation accompany single-strand annealing repair of a dicentric chromosome in Saccharomyces cerevisiaeDouglas A Thrower
Department of Biology, CB3280 University of North Carolina, Chapel Hill, NC 27599 3280, USA
J Cell Sci 116:561-9. 2003..repair requires the adaptation-defective cdc5-ad allele of the yeast polo kinase and the DNA damage checkpoint gene RAD9. Dicentric chromosome breakage in cdc5-ad rad52 mutant cells is associated with a prolonged mitotic arrest, during ..
- Telomerase subunit overexpression suppresses telomere-specific checkpoint activation in the yeast yku80 mutantS H Teo
Wellcome Trust and Cancer Research Campaign, Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge CB2 1QR, UK
EMBO Rep 2:197-202. 2001....
- Yeast telomere capping protein Stn1 overrides DNA replication control through the S phase checkpointHovik J Gasparyan
Department of Cell Biology and Neuroscience, University of California, Biological Sciences Building, Riverside, CA 92521, USA
Proc Natl Acad Sci U S A 106:2206-11. 2009..During replication stress, the mislocalized Stn1 may inappropriately promote Pol alpha in a manner that interferes with Rad53 effector mechanisms controlling replication fork integrity...
- Mutation of Gly721 alters DNA topoisomerase I active site architecture and sensitivity to camptothecinMarie van der Merwe
Department of Molecular Pharmacology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
J Biol Chem 283:3305-15. 2008..We postulate that this conserved Gly residue provides a flexible hinge within the Top1p catalytic pocket to facilitate linker dynamics and the structural alterations that accompany drug binding of the covalent enzyme-DNA intermediate...
- Hst3 is regulated by Mec1-dependent proteolysis and controls the S phase checkpoint and sister chromatid cohesion by deacetylating histone H3 at lysine 56Safia Thaminy
Clinical Research Division and Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
J Biol Chem 282:37805-14. 2007..The pathway that regulates H3K56 acetylation acts in parallel with the Rad9 pathway to transmit a DNA damage signal from Mec1 to Rad53...
- Association with the origin recognition complex suggests a novel role for histone acetyltransferase Hat1p/Hat2pBernhard Suter
Program in Proteomics and Bioinformatics, Banting and Best Department of Medical Genetics, University of Toronto, Toronto, Ontario, Canada
BMC Biol 5:38. 2007..In yeast, a major conserved histone acetyltransferase, Hat1p, preferentially acetylates lysine residues 5 and 12 on histone H4...
- Control of translocations between highly diverged genes by Sgs1, the Saccharomyces cerevisiae homolog of the Bloom's syndrome proteinKristina H Schmidt
Department of Biology, University of South Florida, 4202 E Fowler Avenue, SCA110, Tampa, FL 33620, USA
Mol Cell Biol 26:5406-20. 2006..between diverged genes in cells with mutations in the genes encoding the checkpoint proteins Mec3, Rad24, Rad9, or Rfc5, the chromatin assembly factors Cac1 or Asf1, and the DNA helicase Rrm3...
- Regulation of the Rad53 protein kinase in signal amplification by oligomer assembly and disassemblyNianhan Jia-Lin Ma
Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
Cell Cycle 7:808-17. 2008..The mediator Rad9 is not necessary for Rad53 oligomerization...
- Uncoupling of unwinding from DNA synthesis implies regulation of MCM helicase by Tof1/Mrc1/Csm3 checkpoint complexMarina N Nedelcheva
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria
J Mol Biol 347:509-21. 2005..In concordance with this suggestion, we found that the Tof1/Csm3/Mrc1 checkpoint complex interacts directly with the MCM helicase during both replication fork progression and when the replication fork is stalled...
- Genetic analysis of Saccharomyces cerevisiae H2A serine 129 mutant suggests a functional relationship between H2A and the sister-chromatid cohesion partners Csm3-Tof1 for the repair of topoisomerase I-induced DNA damageChristophe Redon
NIH, NCI, DBS, Laboratory of Molecular Pharmacology, Bethesda, Maryland 20892, USA
Genetics 172:67-76. 2006..the pathway involving H2A/Csm3/Tof1 is distinct from the pathways involving the Ctf8/Ctf18/Dcc1 complex, the Rad9 pathway, and another involving Mrc1...
- Mutants defective in Rad1-Rad10-Slx4 exhibit a unique pattern of viability during mating-type switching in Saccharomyces cerevisiaeAmy M Lyndaker
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853 2703, USA
Genetics 179:1807-21. 2008..We found that mutants lacking an intact Rad1-Rad10-Slx4 complex displayed RAD9- and MAD2-dependent cell cycle delays and decreased viability during mating-type switching...
- Mechanisms of cell cycle control revealed by a systematic and quantitative overexpression screen in S. cerevisiaeWei Niu
Center for Systems and Synthetic Biology, University of Texas, Austin, Texas, United States of America
PLoS Genet 4:e1000120. 2008..This work thus implicates new genes in cell cycle progression, complements previous screens, and lays the foundation for future experiments to define more precisely roles for these genes in cell cycle progression...
- The RAD6/BRE1 histone modification pathway in Saccharomyces confers radiation resistance through a RAD51-dependent process that is independent of RAD18John C Game
Life Sciences Division, Lawrence Berkeley National Laboratory, CA 94720, USA
Genetics 173:1951-68. 2006..We conclude that IR resistance conferred by BRE1 and DOT1 is mediated through homologous recombinational repair, not postreplication repair, and confirm findings of a G1 checkpoint role for the RAD6/BRE1/DOT1 pathway...
- The Saccharomyces cerevisiae checkpoint genes RAD9, CHK1 and PDS1 are required for elevated homologous recombination in a mec1 (ATR) hypomorphic mutantMichael Fasullo
Ordway Research Institute, Albany, New York 12208, USA
Cell Cycle 7:2418-26. 2008..The mec1-21 hyper-recombination was partially reduced in rad9, pds1 and chk1 mutants, and abolished in rad52 mutants, suggesting the hyper-recombination results from RAD52-..
- Reconstitution of Rad53 activation by Mec1 through adaptor protein Mrc1Sheng Hong Chen
Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093 0653, USA
J Biol Chem 284:18593-604. 2009..Further, the conserved C-terminal domain of Mrc1 was found to be required for Rad53 activation. These results thus provide insights into the role of the adaptor protein Mrc1 in activating Rad53 in the DNA replication checkpoint...
- Budding yeast 14-3-3 proteins contribute to the robustness of the DNA damage and spindle checkpointsNathalie Grandin
IFR128 BioSciences Gerland, UMR CNRS ENS n degrees 5239, Ecole Normale Superieure de Lyon, Lyon, France
Cell Cycle 7:2749-61. 2008..Therefore, the budding yeast 14-3-3 proteins contribute to the robustness of the two major mitotic checkpoints and, by doing so, may also ensure optimal coordination between the responses to two distinct types of damage...
- Suppression of translocations by RecQ-like DNA helicasesKRISTINA HILDEGARD SCHMIDT; Fiscal Year: 2010....
- Activating liver carcinogens in yeast by expressing CYP450 polymorphismsMichael Fasullo; Fiscal Year: 2007..Ultimately, the information will aid public health practitioners and clinicians to identify which individuals are at highest risk for liver cancer, and guide prevention and early detection efforts. ..
- Activating liver carcinogens in yeast by expressing CYP450 polymorphismsMichael Fasullo; Fiscal Year: 2007....
- Repair Pathways for Ionizing Radiation Damage YeastJohn Game; Fiscal Year: 2006..Recent and existing mutants will be studied in detail with this system to determine the molecular involvement of each gene in DSB repair, sister chromatid exchange and recombination between chromosomes. ..
- RADIATION INDUCTION OF GENOMIC REARRANGEMENTS IN YEASTMichael Fasullo; Fiscal Year: 2004..The purpose of this proposal is to elucidate the genetic pathway by which the Saccharomyces cerevisiae RAD9 checkpoint reduces genetic instability that results from homologous recombination between repeated sequences (..
- REPAIR PATHWAYS FOR IONIZING RADIATION DAMAGE IN YEASTJohn Game; Fiscal Year: 2002..In a related aim, the order of gene function for RAD5O and RAD54 will be tested using different switching regimes with double mutants containing conditional alleles with differing permissive conditions. ..
- Suppression of translocations by RecQ-like DNA helicasesKRISTINA HILDEGARD SCHMIDT; Fiscal Year: 2010....