RAD9

Summary

Gene Symbol: RAD9
Description: chromatin-binding protein RAD9
Alias: chromatin-binding protein RAD9
Species: Saccharomyces cerevisiae S288c
Products:     RAD9

Top Publications

  1. Dion V, Kalck V, Horigome C, Towbin B, Gasser S. Increased mobility of double-strand breaks requires Mec1, Rad9 and the homologous recombination machinery. Nat Cell Biol. 2012;14:502-9 pubmed publisher
    ..mobility requires Rad51, the ATPase activity of Rad54, the ATR homologue Mec1 and the DNA-damage-response mediator Rad9. Consistent with a role for movement in the homology-search step of homologous recombination, we show that ..
  2. Longhese M, Paciotti V, Fraschini R, Zaccarini R, Plevani P, Lucchini G. The novel DNA damage checkpoint protein ddc1p is phosphorylated periodically during the cell cycle and in response to DNA damage in budding yeast. EMBO J. 1997;16:5216-26 pubmed
    ..Moreover, Ddc1p is phosphorylated periodically during a normal cell cycle and becomes hyperphosphorylated in response to DNA damage. Both phosphorylation events are at least partially dependent on a functional MEC3 gene. ..
  3. Vialard J, Gilbert C, Green C, Lowndes N. The budding yeast Rad9 checkpoint protein is subjected to Mec1/Tel1-dependent hyperphosphorylation and interacts with Rad53 after DNA damage. EMBO J. 1998;17:5679-88 pubmed
    The Saccharomyces cerevisiae RAD9 checkpoint gene is required for transient cell-cycle arrests and transcriptional induction of DNA repair genes in response to DNA damage...
  4. Longhese M, Fraschini R, Plevani P, Lucchini G. Yeast pip3/mec3 mutants fail to delay entry into S phase and to slow DNA replication in response to DNA damage, and they define a functional link between Mec3 and DNA primase. Mol Cell Biol. 1996;16:3235-44 pubmed
  5. Emili A. MEC1-dependent phosphorylation of Rad9p in response to DNA damage. Mol Cell. 1998;2:183-9 pubmed
    In budding yeast, DNA damage can activate a checkpoint surveillance system controlled by the RAD9, RAD53, and MEC1 genes, resulting in a delay in cell cycle progression...
  6. Blankley R, Lydall D. A domain of Rad9 specifically required for activation of Chk1 in budding yeast. J Cell Sci. 2004;117:601-8 pubmed
    The Rad9 protein is a key adaptor protein in Saccharomyces cerevisiae DNA damage checkpoint pathways...
  7. Chen X, Cui D, Papusha A, Zhang X, Chu C, Tang J, et al. The Fun30 nucleosome remodeller promotes resection of DNA double-strand break ends. Nature. 2012;489:576-80 pubmed publisher
    ..Fun30 becomes less important for resection in the absence of the histone-bound Rad9 checkpoint adaptor protein known to block 5' strand processing and in the absence of either histone H3 K79 ..
  8. Sun Z, Hsiao J, Fay D, Stern D. Rad53 FHA domain associated with phosphorylated Rad9 in the DNA damage checkpoint. Science. 1998;281:272-4 pubmed
    ..The Rad9 protein was phosphorylated in response to DNA damage, and phosphorylated Rad9 interacted with the COOH-terminal ..
  9. NNAKWE C, Altaf M, Cote J, Kron S. Dissection of Rad9 BRCT domain function in the mitotic checkpoint response to telomere uncapping. DNA Repair (Amst). 2009;8:1452-61 pubmed publisher
    ..Throughout the cell cycle, the multi-domain adaptor protein Rad9 is required for the activation of checkpoint effector kinase Rad53 in response to DSBs and is similarly necessary ..

More Information

Publications71

  1. Barbour L, Ball L, Zhang K, Xiao W. DNA damage checkpoints are involved in postreplication repair. Genetics. 2006;174:1789-800 pubmed
    ..sublethal dose of a DNA-damaging agent to identify novel genes involved in PRR, which resulted in the isolation of RAD9 as a candidate PRR gene...
  2. Lee S, Schwartz M, Duong J, Stern D. Rad53 phosphorylation site clusters are important for Rad53 regulation and signaling. Mol Cell Biol. 2003;23:6300-14 pubmed
    ..These substitution mutations spared the basal interaction with Asf1 and the DNA damage-induced interactions with Rad9. However, they caused a decrease in DNA damage-induced Rad53 kinase activity and an impaired interaction with the ..
  3. Usui T, Ogawa H, Petrini J. A DNA damage response pathway controlled by Tel1 and the Mre11 complex. Mol Cell. 2001;7:1255-66 pubmed
    ..In mitotic cells, the Tel1-Mre11 complex pathway triggers Rad53 activation and its interaction with Rad9, whereas in meiosis it acts via Rad9 and the Rad53 paralog Mre4/Mek1...
  4. de La Torre Ruiz M, Lowndes N. The Saccharomyces cerevisiae DNA damage checkpoint is required for efficient repair of double strand breaks by non-homologous end joining. FEBS Lett. 2000;467:311-5 pubmed
    In this work we report that the Saccharomyces cerevisiae RAD9, RAD24, RAD17, MEC1, MEC3 and RAD53 checkpoint genes are required for efficient non-homologous end joining (NHEJ). RAD9 and RAD24 function additionally in this process...
  5. Paulovich A, Armour C, Hartwell L. The Saccharomyces cerevisiae RAD9, RAD17, RAD24 and MEC3 genes are required for tolerating irreparable, ultraviolet-induced DNA damage. Genetics. 1998;150:75-93 pubmed
    ..We find that RAD9, RAD17, RAD24, and MEC3 are required for UV-induced (although not spontaneous) mutagenesis, and that RAD9 and RAD17 ..
  6. Lazzaro F, Sapountzi V, Granata M, Pellicioli A, Vaze M, Haber J, et al. Histone methyltransferase Dot1 and Rad9 inhibit single-stranded DNA accumulation at DSBs and uncapped telomeres. EMBO J. 2008;27:1502-12 pubmed publisher
    ..Here, we provide evidence that binding of the checkpoint protein Rad9, through its Tudor domain, to methylated histone H3-K79 inhibits resection at DSBs and uncapped telomeres...
  7. Al Moghrabi N, Al Sharif I, Aboussekhra A. The RAD9-dependent gene trans-activation is required for excision repair of active genes but not for repair of non-transcribed DNA. Mutat Res. 2009;663:60-8 pubmed publisher
    The Saccharomyces cerevisiae RAD9 and RAD24 are two cell cycle checkpoint genes required for UV-dependent up-regulation of a battery of genes involved in different metabolic pathways...
  8. Grandin N, Damon C, Charbonneau M. Cdc13 prevents telomere uncapping and Rad50-dependent homologous recombination. EMBO J. 2001;20:6127-39 pubmed
    ..We propose that Cdc13 prevents telomere uncapping and inhibits recombination between telomeric sequences through a pathway distinct from and complementary to that used by telomerase. ..
  9. Wang H, Elledge S. Genetic and physical interactions between DPB11 and DDC1 in the yeast DNA damage response pathway. Genetics. 2002;160:1295-304 pubmed
    ..These results suggest that DPB11 and DDC1 may function in the same or parallel pathways after DNA damage and that DDC1 may play a role in responding to replication defects. ..
  10. Garvik B, Carson M, Hartwell L. Single-stranded DNA arising at telomeres in cdc13 mutants may constitute a specific signal for the RAD9 checkpoint. Mol Cell Biol. 1995;15:6128-38 pubmed
    ..in the G2 phase of the cell cycle at the restrictive temperature as a result of DNA damage that activates the RAD9 checkpoint...
  11. Hishida T, Kubota Y, Carr A, Iwasaki H. RAD6-RAD18-RAD5-pathway-dependent tolerance to chronic low-dose ultraviolet light. Nature. 2009;457:612-5 pubmed publisher
    ..Thus, the error-free PRR pathway is specifically important during chronic low-dose ultraviolet exposure to prevent counter-productive DNA checkpoint activation and allow cells to proliferate normally. ..
  12. Lee S, Moore J, Holmes A, Umezu K, Kolodner R, Haber J. Saccharomyces Ku70, mre11/rad50 and RPA proteins regulate adaptation to G2/M arrest after DNA damage. Cell. 1998;94:399-409 pubmed
    ..hdf1 cells, lacking Ku70p, fail to escape from this RAD9/RAD17-dependent checkpoint...
  13. Soulier J, Lowndes N. The BRCT domain of the S. cerevisiae checkpoint protein Rad9 mediates a Rad9-Rad9 interaction after DNA damage. Curr Biol. 1999;9:551-4 pubmed
    The Saccharomyces cerevisiae checkpoint protein Rad9 is required for transient cell-cycle arrest and transcriptional induction of DNA-repair genes in response to DNA damage [1]...
  14. Lee S, Pellicioli A, Malkova A, Foiani M, Haber J. The Saccharomyces recombination protein Tid1p is required for adaptation from G2/M arrest induced by a double-strand break. Curr Biol. 2001;11:1053-7 pubmed
    ..mutations yku70Delta and cdc5-ad, permanent arrest in tid1Delta is bypassed by the deletion of the checkpoint gene RAD9. Permanent arrest of tid1Delta cells is suppressed by the rfa1-t11 mutation in the ssDNA binding complex RPA, ..
  15. Wysocki R, Javaheri A, Allard S, Sha F, Cote J, Kron S. Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9. Mol Cell Biol. 2005;25:8430-43 pubmed
    ..Consistent with this paradigm, loss of Dot1 prevents activation of the yeast 53BP1 ortholog Rad9 or Chk2 homolog Rad53 and decreases binding of Rad9 to DSBs after DNA damage...
  16. Schwartz M, Lee S, Duong J, Eminaga S, Stern D. FHA domain-mediated DNA checkpoint regulation of Rad53. Cell Cycle. 2003;2:384-96 pubmed
    ..Both FHA1 and FHA2 are required for the robust activation of Rad53 by the RAD9-dependent DNA damage checkpoint pathway, while an intact FHA1 or FHA2 allows the activation of Rad53 in response to ..
  17. Maringele L, Lydall D. EXO1-dependent single-stranded DNA at telomeres activates subsets of DNA damage and spindle checkpoint pathways in budding yeast yku70Delta mutants. Genes Dev. 2002;16:1919-33 pubmed
    ..We show that CHK1, MEC1, and RAD9 checkpoint genes are required for efficient cell cycle arrest of yku70Delta mutants cultured at 37 degrees C, ..
  18. Leroy C, Lee S, Vaze M, Ochsenbein F, Ochsenbien F, Guerois R, et al. PP2C phosphatases Ptc2 and Ptc3 are required for DNA checkpoint inactivation after a double-strand break. Mol Cell. 2003;11:827-35 pubmed
    ..In vivo and in vitro evidence suggests that phosphorylated forms of Ptc2 and Ptc3 specifically bind to the Rad53 FHA1 domain and inactivate Rad53-dependent pathways during adaptation and recovery by dephosphorylating Rad53. ..
  19. Gardner R, Putnam C, Weinert T. RAD53, DUN1 and PDS1 define two parallel G2/M checkpoint pathways in budding yeast. EMBO J. 1999;18:3173-85 pubmed
    ..A current model posits three gene classes: those encoding proteins acting on damaged DNA (e.g. RAD9 and RAD24), those transducing a signal (MEC1, RAD53 and DUN1) or those participating more directly in arrest (PDS1)...
  20. Pike B, Yongkiettrakul S, Tsai M, Heierhorst J. Mdt1, a novel Rad53 FHA1 domain-interacting protein, modulates DNA damage tolerance and G(2)/M cell cycle progression in Saccharomyces cerevisiae. Mol Cell Biol. 2004;24:2779-88 pubmed
    ..The data indicate that Mdt1 is involved in normal G(2)/M cell cycle progression and is a novel target of checkpoint-dependent cell cycle arrest pathways. ..
  21. Scott K, Plon S. Loss of Sin3/Rpd3 histone deacetylase restores the DNA damage response in checkpoint-deficient strains of Saccharomyces cerevisiae. Mol Cell Biol. 2003;23:4522-31 pubmed
    ..Deletion of either SIN3 or RPD3 in rad9 or mec1 checkpoint mutant strains suppresses sensitivity to replication blocks and DNA damage resulting from Cdc9 ..
  22. Kaochar S, Shanks L, Weinert T. Checkpoint genes and Exo1 regulate nearby inverted repeat fusions that form dicentric chromosomes in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2010;107:21605-10 pubmed publisher
    ..inhibit Exo1 in one pathway, whereas in a second pathway the ATR-like kinases Mec1 and Tel1, adaptor protein Rad9, and effector kinases Chk1 and Dun1 act independently of Exo1 to prevent inverted repeat fusion...
  23. Pfander B, Diffley J. Dpb11 coordinates Mec1 kinase activation with cell cycle-regulated Rad9 recruitment. EMBO J. 2011;30:4897-907 pubmed publisher
    ..Here, we show that a ternary complex of Dpb11, Mec1 and another key mediator protein Rad9 is required for efficient Rad9 phosphorylation by Mec1 in vitro, and for checkpoint activation in vivo...
  24. Chen Y, Caldwell J, Pereira E, Baker R, Sanchez Y. ATRMec1 phosphorylation-independent activation of Chk1 in vivo. J Biol Chem. 2009;284:182-90 pubmed publisher
    ..Our findings show that a single amino acid substitution in the C terminus, which could lead to an allosteric change in Chk1, allows it to bypass the requirement of the conserved ATR(Mec1) phosphorylation sites for checkpoint function. ..
  25. Torres J, Schnakenberg S, Zakian V. Saccharomyces cerevisiae Rrm3p DNA helicase promotes genome integrity by preventing replication fork stalling: viability of rrm3 cells requires the intra-S-phase checkpoint and fork restart activities. Mol Cell Biol. 2004;24:3198-212 pubmed
    ..The rrm3 system provides a unique opportunity to learn the fate of forks whose progress is impaired by natural impediments rather than by exogenous DNA damage. ..
  26. Zou L, Liu D, Elledge S. Replication protein A-mediated recruitment and activation of Rad17 complexes. Proc Natl Acad Sci U S A. 2003;100:13827-32 pubmed
    The human Rad17-Rfc2-5 and Rad9-Rad1-Hus1 complexes play crucial roles in the activation of the ATR-mediated DNA damage and DNA replication stress response pathways...
  27. Schwartz M, Duong J, Sun Z, Morrow J, Pradhan D, Stern D. Rad9 phosphorylation sites couple Rad53 to the Saccharomyces cerevisiae DNA damage checkpoint. Mol Cell. 2002;9:1055-65 pubmed
    b>Rad9 is required for the MEC1/TEL1-dependent activation of Saccharomyces cerevisiae DNA damage checkpoint pathways mediated by Rad53 and Chk1...
  28. Deshpande A, Ivanova I, Raykov V, Xue Y, Maringele L. Polymerase epsilon is required to maintain replicative senescence. Mol Cell Biol. 2011;31:1637-45 pubmed publisher
    ..time with an Mrc1-regulated Pol ? resynthesis of a short, double-stranded chromosome end, which in turn activates a Rad9(53BP1)-dependent checkpoint pathway...
  29. Huang D, Piening B, Paulovich A. The preference for error-free or error-prone postreplication repair in Saccharomyces cerevisiae exposed to low-dose methyl methanesulfonate is cell cycle dependent. Mol Cell Biol. 2013;33:1515-27 pubmed publisher
    ..However, when PRR is restricted to the G2 phase, cells utilize REV3-dependent translesion synthesis, which requires a MEC1-dependent delay and results in significant hypermutability. ..
  30. Vidanes G, Sweeney F, Galicia S, Cheung S, Doyle J, Durocher D, et al. CDC5 inhibits the hyperphosphorylation of the checkpoint kinase Rad53, leading to checkpoint adaptation. PLoS Biol. 2010;8:e1000286 pubmed publisher
    ..Mec1 appeared to be active, since the Rad9 adaptor retained its Mec1 phosphorylation...
  31. Lisby M, Barlow J, Burgess R, Rothstein R. Choreography of the DNA damage response: spatiotemporal relationships among checkpoint and repair proteins. Cell. 2004;118:699-713 pubmed
  32. Game J, Williamson M, Spicakova T, Brown J. The RAD6/BRE1 histone modification pathway in Saccharomyces confers radiation resistance through a RAD51-dependent process that is independent of RAD18. Genetics. 2006;173:1951-68 pubmed
    ..We conclude that IR resistance conferred by BRE1 and DOT1 is mediated through homologous recombinational repair, not postreplication repair, and confirm findings of a G1 checkpoint role for the RAD6/BRE1/DOT1 pathway. ..
  33. Paschini M, Toro T, Lubin J, Braunstein Ballew B, Morris D, Lundblad V. A naturally thermolabile activity compromises genetic analysis of telomere function in Saccharomyces cerevisiae. Genetics. 2012;191:79-93 pubmed publisher
    ..one of these new cdc13-ts alleles argues that the accelerated inviability previously observed at 36° in cdc13-1 rad9-? mutant strains is a consequence of the Tmp(-) phenotype...
  34. Karras G, Fumasoni M, Sienski G, Vanoli F, Branzei D, Jentsch S. Noncanonical role of the 9-1-1 clamp in the error-free DNA damage tolerance pathway. Mol Cell. 2013;49:536-46 pubmed publisher
    ..Our findings thus reveal unexpected cooperation in the error-free pathway between the two related clamps and indicate that 9-1-1 plays a broader role in the DNA damage response than previously assumed. ..
  35. Hoch N, Chen E, Buckland R, Wang S, Fazio A, Hammet A, et al. Molecular basis of the essential s phase function of the rad53 checkpoint kinase. Mol Cell Biol. 2013;33:3202-13 pubmed publisher
    ..mutant, lacking the N-terminal Mec1 phosphorylation site cluster, is synthetic lethal with a deletion of the RAD9 DNA damage checkpoint adaptor...
  36. Foss E. Tof1p regulates DNA damage responses during S phase in Saccharomyces cerevisiae. Genetics. 2001;157:567-77 pubmed
    ..that mutants missing this branch are particularly dependent on the cell cycle-wide branch and, therefore, on RAD9, for surviving DNA damage...
  37. Tanaka H, Katou Y, Yagura M, Saitoh K, Itoh T, Araki H, et al. Ctf4 coordinates the progression of helicase and DNA polymerase alpha. Genes Cells. 2009;14:807-20 pubmed publisher
    ..These results lead us to propose that Ctf4 is a key connector between DNA helicase and Pol alpha and is required for the coordinated progression of the replisome. ..
  38. Sweeney F, Yang F, Chi A, Shabanowitz J, Hunt D, Durocher D. Saccharomyces cerevisiae Rad9 acts as a Mec1 adaptor to allow Rad53 activation. Curr Biol. 2005;15:1364-75 pubmed
    ..To elucidate the mechanisms that underlie the MEC1 and RAD9-dependent activation of Rad53, the Saccharomyces cerevisiae ortholog of Chk2, we mapped and characterized in vivo ..
  39. Hammet A, Magill C, Heierhorst J, Jackson S. Rad9 BRCT domain interaction with phosphorylated H2AX regulates the G1 checkpoint in budding yeast. EMBO Rep. 2007;8:851-7 pubmed
    ..Furthermore, we show that the tandem BRCT domain of Rad9 interacts directly with phosphorylated H2A in vitro and that a rad9 point mutation that abolishes this interaction ..
  40. Myung K, Kolodner R. Suppression of genome instability by redundant S-phase checkpoint pathways in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2002;99:4500-7 pubmed
    ..These data support the view that spontaneous genome rearrangements result from DNA replication errors and indicate that there is a high degree of redundancy among the checkpoints that act in S phase to suppress such genome instability. ..
  41. Osborn A, Elledge S. Mrc1 is a replication fork component whose phosphorylation in response to DNA replication stress activates Rad53. Genes Dev. 2003;17:1755-67 pubmed
  42. Granata M, Lazzaro F, Novarina D, Panigada D, Puddu F, Abreu C, et al. Dynamics of Rad9 chromatin binding and checkpoint function are mediated by its dimerization and are cell cycle-regulated by CDK1 activity. PLoS Genet. 2010;6: pubmed publisher
    Saccharomyces cerevisiae Rad9 is required for an effective DNA damage response throughout the cell cycle...
  43. de La Torre Ruiz M, Green C, Lowndes N. RAD9 and RAD24 define two additive, interacting branches of the DNA damage checkpoint pathway in budding yeast normally required for Rad53 modification and activation. EMBO J. 1998;17:2687-98 pubmed
    In budding yeast, RAD9 and RAD24/RAD17/MEC3 are believed to function upstream of MEC1 and RAD53 in signalling the presence of DNA damage...
  44. Paulovich A, Margulies R, Garvik B, Hartwell L. RAD9, RAD17, and RAD24 are required for S phase regulation in Saccharomyces cerevisiae in response to DNA damage. Genetics. 1997;145:45-62 pubmed
    ..In this report, we show that other genes (RAD9, RAD17, RAD24) involved in the DNA damage checkpoint pathway also play a role in regulating S phase in response to ..
  45. Chen S, Zhou H. Reconstitution of Rad53 activation by Mec1 through adaptor protein Mrc1. J Biol Chem. 2009;284:18593-604 pubmed publisher
    ..Further, the conserved C-terminal domain of Mrc1 was found to be required for Rad53 activation. These results thus provide insights into the role of the adaptor protein Mrc1 in activating Rad53 in the DNA replication checkpoint. ..
  46. Ball L, Zhang K, Cobb J, Boone C, Xiao W. The yeast Shu complex couples error-free post-replication repair to homologous recombination. Mol Microbiol. 2009;73:89-102 pubmed publisher
    ..This mechanism appears to be conserved throughout eukaryotes. ..
  47. Foster S, Zubko M, Guillard S, Lydall D. MRX protects telomeric DNA at uncapped telomeres of budding yeast cdc13-1 mutants. DNA Repair (Amst). 2006;5:840-51 pubmed
    ..Instead, we find that Rad50 inhibits ssDNA accumulation and promotes cdc13-1 cell viability, consistent with a major role for MRX in telomere capping. ..
  48. Toh G, O Shaughnessy A, Jimeno S, Dobbie I, Grenon M, Maffini S, et al. Histone H2A phosphorylation and H3 methylation are required for a novel Rad9 DSB repair function following checkpoint activation. DNA Repair (Amst). 2006;5:693-703 pubmed
    In budding yeast, the Rad9 protein is an important player in the maintenance of genomic integrity and has a well-characterised role in DNA damage checkpoint activation...
  49. Vodenicharov M, Wellinger R. DNA degradation at unprotected telomeres in yeast is regulated by the CDK1 (Cdc28/Clb) cell-cycle kinase. Mol Cell. 2006;24:127-37 pubmed
    ..These results strongly suggest that after a loss of the telomere capping function, telomere-led genome instability is caused by tightly regulated cellular DNA repair attempts. ..
  50. Lydall D, Weinert T. Yeast checkpoint genes in DNA damage processing: implications for repair and arrest. Science. 1995;270:1488-91 pubmed
    ..Another checkpoint gene, RAD9, had a different role: It inhibited the degradation by RAD17, RAD24, and MEC3...
  51. Trovesi C, Falcettoni M, Lucchini G, Clerici M, Longhese M. Distinct Cdk1 requirements during single-strand annealing, noncrossover, and crossover recombination. PLoS Genet. 2011;7:e1002263 pubmed publisher
    ..This ability to perform SSA depends on DSB resection, because both resection and SSA are enhanced by the lack of Rad9 in yku70? G1 cells...
  52. Lancelot N, Charier G, Couprie J, Duband Goulet I, Alpha Bazin B, Quemeneur E, et al. The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNA. Nucleic Acids Res. 2007;35:5898-912 pubmed
    ..Budding yeast Rad9, fission yeast Crb2 and metazoan 53BP1 are presented as mediators involved in the activation of checkpoint kinases...
  53. Crabbe L, Thomas A, Pantesco V, de Vos J, Pasero P, Lengronne A. Analysis of replication profiles reveals key role of RFC-Ctf18 in yeast replication stress response. Nat Struct Mol Biol. 2010;17:1391-7 pubmed publisher
    ..These data identify RFC(Ctf18) as a key DRC mediator, potentially bridging Mrc1 and primed ssDNA to signal paused forks. ..
  54. Ngo H, Lydall D. Survival and growth of yeast without telomere capping by Cdc13 in the absence of Sgs1, Exo1, and Rad9. PLoS Genet. 2010;6:e1001072 pubmed publisher
    ..We found that simultaneous inactivation of Sgs1, Exo1, and Rad9, three DNA damage response (DDR) proteins, is sufficient to allow cell division in the absence of Cdc13...
  55. Usui T, Foster S, Petrini J. Maintenance of the DNA-damage checkpoint requires DNA-damage-induced mediator protein oligomerization. Mol Cell. 2009;33:147-59 pubmed publisher
    ..cerevisiae BRCT protein Rad9. Our data suggest that Rad9's tandem BRCT domain mediates Rad9 oligomerization via its interaction with its own ..
  56. Gilbert C, Green C, Lowndes N. Budding yeast Rad9 is an ATP-dependent Rad53 activating machine. Mol Cell. 2001;8:129-36 pubmed
    We find budding yeast Rad9 in two distinct, large, and soluble complexes in cell extracts...
  57. Yu S, Teng Y, Lowndes N, Waters R. RAD9, RAD24, RAD16 and RAD26 are required for the inducible nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers from the transcribed and non-transcribed regions of the Saccharomyces cerevisiae MFA2 gene. Mutat Res. 2001;485:229-36 pubmed
    ..No inducible repair was observed in rad9, rad24, rad16 and rad26 cells, indicating two checkpoint genes RAD9 and RAD24, the global repair gene RAD16 and the ..
  58. Anbalagan S, Bonetti D, Lucchini G, Longhese M. Rif1 supports the function of the CST complex in yeast telomere capping. PLoS Genet. 2011;7:e1002024 pubmed publisher
    ..Thus, these data highlight a novel role for Rif1 in assisting the essential telomere protection function of the CST complex. ..
  59. Pike B, Tenis N, Heierhorst J. Rad53 kinase activation-independent replication checkpoint function of the N-terminal forkhead-associated (FHA1) domain. J Biol Chem. 2004;279:39636-44 pubmed
  60. Zubko M, Guillard S, Lydall D. Exo1 and Rad24 differentially regulate generation of ssDNA at telomeres of Saccharomyces cerevisiae cdc13-1 mutants. Genetics. 2004;168:103-15 pubmed
    ..We show that Exo1 is unique among the repair genes tested because like Rad9 and Rad24 checkpoint proteins, Exo1 inhibits the growth of cdc13-1 mutants at the semipermissive temperatures...
  61. Petreaca R, Chiu H, Nugent C. The role of Stn1p in Saccharomyces cerevisiae telomere capping can be separated from its interaction with Cdc13p. Genetics. 2007;177:1459-74 pubmed
    ..Thus, an amino-terminal region of Stn1p is sufficient for its essential function, while a central region of Stn1p either negatively regulates the STN1 essential function or destabilizes the mutant Stn1 protein. ..
  62. Schiestl R, Reynolds P, Prakash S, Prakash L. Cloning and sequence analysis of the Saccharomyces cerevisiae RAD9 gene and further evidence that its product is required for cell cycle arrest induced by DNA damage. Mol Cell Biol. 1989;9:1882-96 pubmed
    ..Here, we provide evidence that the Saccharomyces cerevisiae RAD9 gene, mutations of which confer sensitivity to DNA-damaging agents, is necessary for the cell cycle arrest ..