RAD9

Summary

Gene Symbol: RAD9
Description: Rad9p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. pmc Dpb11 coordinates Mec1 kinase activation with cell cycle-regulated Rad9 recruitment
    Boris Pfander
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire, UK
    EMBO J 30:4897-907. 2011
  2. pmc Rif1 supports the function of the CST complex in yeast telomere capping
    Savani Anbalagan
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
    PLoS Genet 7:e1002024. 2011
  3. pmc Maintenance of the DNA-damage checkpoint requires DNA-damage-induced mediator protein oligomerization
    Takehiko Usui
    Laboratory of Chromosome Biology, Sloan Kettering Institute, New York, NY 10065, USA
    Mol Cell 33:147-59. 2009
  4. doi Ctf4 coordinates the progression of helicase and DNA polymerase alpha
    Hirokazu Tanaka
    Laboratory of Chromosome Structure and Function, Department of Biological Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama City, Kanagawa, Japan
    Genes Cells 14:807-20. 2009
  5. pmc DNA damage checkpoints are involved in postreplication repair
    Leslie Barbour
    Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada
    Genetics 174:1789-800. 2006
  6. pmc Rad9 BRCT domain interaction with phosphorylated H2AX regulates the G1 checkpoint in budding yeast
    Andrew Hammet
    Wellcome Trust and Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    EMBO Rep 8:851-7. 2007
  7. pmc ATRMec1 phosphorylation-independent activation of Chk1 in vivo
    Yinhuai Chen
    Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0524, USA
    J Biol Chem 284:182-90. 2009
  8. pmc Checkpoint genes and Exo1 regulate nearby inverted repeat fusions that form dicentric chromosomes in Saccharomyces cerevisiae
    Salma Kaochar
    Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721, USA
    Proc Natl Acad Sci U S A 107:21605-10. 2010
  9. ncbi FHA domain-mediated DNA checkpoint regulation of Rad53
    Marc F Schwartz
    Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Cell Cycle 2:384-96. 2003
  10. pmc Dynamics of Rad9 chromatin binding and checkpoint function are mediated by its dimerization and are cell cycle-regulated by CDK1 activity
    Magda Granata
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Milano, Italy
    PLoS Genet 6:. 2010

Scientific Experts

  • Ada Collura
  • Linda J Reha-Krantz
  • Bidyut K Mohanty
  • Bernhard Suter
  • KRISTINA HILDEGARD SCHMIDT
  • D Pei
  • Takashi Hishida
  • John Game
  • A Emili
  • T Formosa
  • E J Foss
  • MICHAEL T FASULLO
  • Brietta L Pike
  • David Lydall
  • N A Koltovaia
  • Marc F Schwartz
  • N F Lowndes
  • Bryan M O'Neill
  • Mikhajlo K Zubko
  • Salma Kaochar
  • Richard D Kolodner
  • Magda Granata
  • Yolanda Sanchez
  • David F Stern
  • Yinhuai Chen
  • Federico Lazzaro
  • Julie M Caldwell
  • Lindsay G Ball
  • Ruben C Petreaca
  • Daniel Durocher
  • Steven S Foster
  • Frederic D Sweeney
  • Marco Muzi-Falconi
  • Paolo Plevani
  • Kyungjae Myung
  • Sandrine Guillard
  • James E Haber
  • Floyd E Romesberg
  • Savani Anbalagan
  • Takashi Kubota
  • Ming Ta Lee
  • Boris Pfander
  • Jocelyn K Nolt
  • Jennifer S Searle
  • Genevieve M Vidanes
  • Hien Ping Ngo
  • Laure Crabbe
  • Mi Sun Kang
  • Andrew L Paek
  • Laura Maringele
  • María Moriel-Carretero
  • Christopher D Putnam
  • Jeff Bachant
  • Ted Weinert
  • Giovanna Lucchini
  • Maria Pia Longhese
  • Soo Jung Lee
  • Andres Aguilera
  • Sang Eun Lee
  • Hovik J Gasparyan
  • Takehiko Usui
  • CHINONYE C NNAKWE
  • Denise Hanway
  • Xuefeng Chen
  • Jimmy K Duong
  • Hirokazu Tanaka
  • Hélène Gaillard
  • Fei Long Meng
  • Nisreen M Al-Moghrabi
  • S E Lee
  • Sheng Hong Chen
  • Stephen J Elledge
  • Takashi Ohuchi
  • Nianhan Jia-Lin Ma
  • Noel F Lowndes
  • Muriel Grenon
  • Marie van der Merwe
  • Veronica Baldo
  • Amy M Lyndaker
  • Nathalie Grandin
  • Constance I Nugent
  • A B Devin
  • Iu V Nikulushkina
  • Sarah Galicia
  • Oscar M Aparicio
  • Wei Niu
  • M P Longhese
  • Wei Xiao
  • Ke Zhang
  • Jacques Cote

Detail Information

Publications114 found, 100 shown here

  1. pmc Dpb11 coordinates Mec1 kinase activation with cell cycle-regulated Rad9 recruitment
    Boris Pfander
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire, UK
    EMBO J 30:4897-907. 2011
    ..Here, we show that a ternary complex of Dpb11, Mec1 and another key mediator protein Rad9 is required for efficient Rad9 phosphorylation by Mec1 in vitro, and for checkpoint activation in vivo...
  2. pmc Rif1 supports the function of the CST complex in yeast telomere capping
    Savani Anbalagan
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
    PLoS Genet 7:e1002024. 2011
    ..Thus, these data highlight a novel role for Rif1 in assisting the essential telomere protection function of the CST complex...
  3. pmc Maintenance of the DNA-damage checkpoint requires DNA-damage-induced mediator protein oligomerization
    Takehiko Usui
    Laboratory of Chromosome Biology, Sloan Kettering Institute, New York, NY 10065, USA
    Mol Cell 33:147-59. 2009
    ..cerevisiae BRCT protein Rad9. Our data suggest that Rad9's tandem BRCT domain mediates Rad9 oligomerization via its interaction with its own ..
  4. doi Ctf4 coordinates the progression of helicase and DNA polymerase alpha
    Hirokazu Tanaka
    Laboratory of Chromosome Structure and Function, Department of Biological Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama City, Kanagawa, Japan
    Genes Cells 14:807-20. 2009
    ..These results lead us to propose that Ctf4 is a key connector between DNA helicase and Pol alpha and is required for the coordinated progression of the replisome...
  5. pmc DNA damage checkpoints are involved in postreplication repair
    Leslie Barbour
    Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada
    Genetics 174:1789-800. 2006
    ..sublethal dose of a DNA-damaging agent to identify novel genes involved in PRR, which resulted in the isolation of RAD9 as a candidate PRR gene...
  6. pmc Rad9 BRCT domain interaction with phosphorylated H2AX regulates the G1 checkpoint in budding yeast
    Andrew Hammet
    Wellcome Trust and Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    EMBO Rep 8:851-7. 2007
    ..Furthermore, we show that the tandem BRCT domain of Rad9 interacts directly with phosphorylated H2A in vitro and that a rad9 point mutation that abolishes this interaction ..
  7. pmc ATRMec1 phosphorylation-independent activation of Chk1 in vivo
    Yinhuai Chen
    Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0524, USA
    J Biol Chem 284:182-90. 2009
    ..Our findings show that a single amino acid substitution in the C terminus, which could lead to an allosteric change in Chk1, allows it to bypass the requirement of the conserved ATR(Mec1) phosphorylation sites for checkpoint function...
  8. pmc Checkpoint genes and Exo1 regulate nearby inverted repeat fusions that form dicentric chromosomes in Saccharomyces cerevisiae
    Salma Kaochar
    Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721, USA
    Proc Natl Acad Sci U S A 107:21605-10. 2010
    ..inhibit Exo1 in one pathway, whereas in a second pathway the ATR-like kinases Mec1 and Tel1, adaptor protein Rad9, and effector kinases Chk1 and Dun1 act independently of Exo1 to prevent inverted repeat fusion...
  9. ncbi FHA domain-mediated DNA checkpoint regulation of Rad53
    Marc F Schwartz
    Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Cell Cycle 2:384-96. 2003
    ..Both FHA1 and FHA2 are required for the robust activation of Rad53 by the RAD9-dependent DNA damage checkpoint pathway, while an intact FHA1 or FHA2 allows the activation of Rad53 in response to ..
  10. pmc Dynamics of Rad9 chromatin binding and checkpoint function are mediated by its dimerization and are cell cycle-regulated by CDK1 activity
    Magda Granata
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Milano, Italy
    PLoS Genet 6:. 2010
    Saccharomyces cerevisiae Rad9 is required for an effective DNA damage response throughout the cell cycle...
  11. pmc Survival and growth of yeast without telomere capping by Cdc13 in the absence of Sgs1, Exo1, and Rad9
    Hien Ping Ngo
    Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, United Kingdom
    PLoS Genet 6:e1001072. 2010
    ..We found that simultaneous inactivation of Sgs1, Exo1, and Rad9, three DNA damage response (DDR) proteins, is sufficient to allow cell division in the absence of Cdc13...
  12. ncbi Choreography of the DNA damage response: spatiotemporal relationships among checkpoint and repair proteins
    Michael Lisby
    Department of Genetics and Development, Columbia University, College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032, USA
    Cell 118:699-713. 2004
    ....
  13. pmc RAD53, DUN1 and PDS1 define two parallel G2/M checkpoint pathways in budding yeast
    R Gardner
    Department of Molecular and Cellular Biology, The University of Arizona, PO Box 21016, Tucson, AZ 85721 0106, USA
    EMBO J 18:3173-85. 1999
    ..A current model posits three gene classes: those encoding proteins acting on damaged DNA (e.g. RAD9 and RAD24), those transducing a signal (MEC1, RAD53 and DUN1) or those participating more directly in arrest (PDS1)...
  14. ncbi The Saccharomyces cerevisiae DNA damage checkpoint is required for efficient repair of double strand breaks by non-homologous end joining
    M de la Torre-Ruiz
    Imperial Cancer Research Fund, Clare Hall Laboratories, CDC Laboratory, South Mimms, UK
    FEBS Lett 467:311-5. 2000
    In this work we report that the Saccharomyces cerevisiae RAD9, RAD24, RAD17, MEC1, MEC3 and RAD53 checkpoint genes are required for efficient non-homologous end joining (NHEJ). RAD9 and RAD24 function additionally in this process...
  15. pmc Dissection of Rad9 BRCT domain function in the mitotic checkpoint response to telomere uncapping
    CHINONYE C NNAKWE
    Department of Pathology, The University of Chicago, Chicago, IL 60637, USA
    DNA Repair (Amst) 8:1452-61. 2009
    ..Throughout the cell cycle, the multi-domain adaptor protein Rad9 is required for the activation of checkpoint effector kinase Rad53 in response to DSBs and is similarly necessary ..
  16. pmc Mrc1 is a replication fork component whose phosphorylation in response to DNA replication stress activates Rad53
    Alexander J Osborn
    Verna and Marrs MacLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Genes Dev 17:1755-67. 2003
    ....
  17. ncbi The Saccharomyces recombination protein Tid1p is required for adaptation from G2/M arrest induced by a double-strand break
    S E Lee
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454, USA
    Curr Biol 11:1053-7. 2001
    ..mutations yku70Delta and cdc5-ad, permanent arrest in tid1Delta is bypassed by the deletion of the checkpoint gene RAD9. Permanent arrest of tid1Delta cells is suppressed by the rfa1-t11 mutation in the ssDNA binding complex RPA, ..
  18. pmc Genetic and physical interactions between DPB11 and DDC1 in the yeast DNA damage response pathway
    Hong Wang
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology and Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Genetics 160:1295-304. 2002
    ..These results suggest that DPB11 and DDC1 may function in the same or parallel pathways after DNA damage and that DDC1 may play a role in responding to replication defects...
  19. pmc The novel DNA damage checkpoint protein ddc1p is phosphorylated periodically during the cell cycle and in response to DNA damage in budding yeast
    M P Longhese
    Dipartimento di Genetica e di Biologia dei Microrganismi, Universita degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy
    EMBO J 16:5216-26. 1997
    ..Moreover, Ddc1p is phosphorylated periodically during a normal cell cycle and becomes hyperphosphorylated in response to DNA damage. Both phosphorylation events are at least partially dependent on a functional MEC3 gene...
  20. pmc RAD9 and RAD24 define two additive, interacting branches of the DNA damage checkpoint pathway in budding yeast normally required for Rad53 modification and activation
    M A de la Torre-Ruiz
    Imperial Cancer Research Fund, Clare Hall Laboratories, CDC Laboratory, South Mimms, Herts EN6 3LD, UK
    EMBO J 17:2687-98. 1998
    In budding yeast, RAD9 and RAD24/RAD17/MEC3 are believed to function upstream of MEC1 and RAD53 in signalling the presence of DNA damage...
  21. pmc Cloning and sequence analysis of the Saccharomyces cerevisiae RAD9 gene and further evidence that its product is required for cell cycle arrest induced by DNA damage
    R H Schiestl
    Department of Biology, University of Rochester, River Campus Station, New York 14627
    Mol Cell Biol 9:1882-96. 1989
    ..Here, we provide evidence that the Saccharomyces cerevisiae RAD9 gene, mutations of which confer sensitivity to DNA-damaging agents, is necessary for the cell cycle arrest ..
  22. ncbi Yeast checkpoint genes in DNA damage processing: implications for repair and arrest
    D Lydall
    Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721, USA
    Science 270:1488-91. 1995
    ..Another checkpoint gene, RAD9, had a different role: It inhibited the degradation by RAD17, RAD24, and MEC3...
  23. ncbi Saccharomyces Ku70, mre11/rad50 and RPA proteins regulate adaptation to G2/M arrest after DNA damage
    S E Lee
    Rosenstiel Center, Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Cell 94:399-409. 1998
    ..hdf1 cells, lacking Ku70p, fail to escape from this RAD9/RAD17-dependent checkpoint...
  24. doi The yeast Shu complex couples error-free post-replication repair to homologous recombination
    Lindsay G Ball
    Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E5
    Mol Microbiol 73:89-102. 2009
    ..This mechanism appears to be conserved throughout eukaryotes...
  25. pmc Loss of Sin3/Rpd3 histone deacetylase restores the DNA damage response in checkpoint-deficient strains of Saccharomyces cerevisiae
    Kenneth L Scott
    Department of Molecular and Human Genetics, Texas Children s Cancer Center, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 23:4522-31. 2003
    ..Deletion of either SIN3 or RPD3 in rad9 or mec1 checkpoint mutant strains suppresses sensitivity to replication blocks and DNA damage resulting from Cdc9 ..
  26. ncbi PP2C phosphatases Ptc2 and Ptc3 are required for DNA checkpoint inactivation after a double-strand break
    Christophe Leroy
    Service de Biochimie et de Génétique Moléculaire, CEA Saclay, 91191 Gif sur Yvette, Cedex, France
    Mol Cell 11:827-35. 2003
    ..In vivo and in vitro evidence suggests that phosphorylated forms of Ptc2 and Ptc3 specifically bind to the Rad53 FHA1 domain and inactivate Rad53-dependent pathways during adaptation and recovery by dephosphorylating Rad53...
  27. pmc EXO1-dependent single-stranded DNA at telomeres activates subsets of DNA damage and spindle checkpoint pathways in budding yeast yku70Delta mutants
    Laura Maringele
    School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK
    Genes Dev 16:1919-33. 2002
    ..We show that CHK1, MEC1, and RAD9 checkpoint genes are required for efficient cell cycle arrest of yku70Delta mutants cultured at 37 degrees C, ..
  28. ncbi MRX protects telomeric DNA at uncapped telomeres of budding yeast cdc13-1 mutants
    Steven S Foster
    Institute for Ageing and Health, University of Newcastle, Henry Wellcome Laboratory for Biogerontology Research, Newcastle General Hospital, Newcastle upon Tyne NE4 6BE, UK
    DNA Repair (Amst) 5:840-51. 2006
    ..Instead, we find that Rad50 inhibits ssDNA accumulation and promotes cdc13-1 cell viability, consistent with a major role for MRX in telomere capping...
  29. pmc Mdt1, a novel Rad53 FHA1 domain-interacting protein, modulates DNA damage tolerance and G(2)/M cell cycle progression in Saccharomyces cerevisiae
    Brietta L Pike
    St Vincent s Institute of Medical Research, Department of Medicine, St Vincent s Hospital, The University of Melbourne, Fitzroy, Victoria 3065, Australia
    Mol Cell Biol 24:2779-88. 2004
    ..The data indicate that Mdt1 is involved in normal G(2)/M cell cycle progression and is a novel target of checkpoint-dependent cell cycle arrest pathways...
  30. pmc Exo1 and Rad24 differentially regulate generation of ssDNA at telomeres of Saccharomyces cerevisiae cdc13-1 mutants
    Mikhajlo K Zubko
    School of Clinical Medical Sciences Gerontology, University of Newcastle, Henry Wellcome Laboratory for Biogerontology Research, Newcastle upon Tyne, NE4 6BE, UK
    Genetics 168:103-15. 2004
    ..We show that Exo1 is unique among the repair genes tested because like Rad9 and Rad24 checkpoint proteins, Exo1 inhibits the growth of cdc13-1 mutants at the semipermissive temperatures...
  31. ncbi Saccharomyces cerevisiae Rad9 acts as a Mec1 adaptor to allow Rad53 activation
    Frederic D Sweeney
    Centre for Systems Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada
    Curr Biol 15:1364-75. 2005
    ..Despite having identified many components of this signaling cascade, the exact mechanisms by which checkpoint kinases are activated after DNA damage, as well as the role of the checkpoint mediators, remain poorly understood...
  32. ncbi DNA degradation at unprotected telomeres in yeast is regulated by the CDK1 (Cdc28/Clb) cell-cycle kinase
    Momchil D Vodenicharov
    Department of Microbiology, Faculty of Medicine, Universite de Sherbrooke, 3001 12e Ave Nord, Sherbrooke, Quebec J1H 5N4, Canada
    Mol Cell 24:127-37. 2006
    ..These results strongly suggest that after a loss of the telomere capping function, telomere-led genome instability is caused by tightly regulated cellular DNA repair attempts...
  33. pmc Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9
    Robert Wysocki
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA
    Mol Cell Biol 25:8430-43. 2005
    ..Consistent with this paradigm, loss of Dot1 prevents activation of the yeast 53BP1 ortholog Rad9 or Chk2 homolog Rad53 and decreases binding of Rad9 to DSBs after DNA damage...
  34. pmc The budding yeast Rad9 checkpoint protein is subjected to Mec1/Tel1-dependent hyperphosphorylation and interacts with Rad53 after DNA damage
    J E Vialard
    Imperial Cancer Research Fund, Clare Hall Laboratories, CDC Laboratory, South Mimms, Hertfordshire EN6 3LD, UK
    EMBO J 17:5679-88. 1998
    The Saccharomyces cerevisiae RAD9 checkpoint gene is required for transient cell-cycle arrests and transcriptional induction of DNA repair genes in response to DNA damage...
  35. ncbi Rad53 FHA domain associated with phosphorylated Rad9 in the DNA damage checkpoint
    Z Sun
    Department of Biology, Yale University, New Haven, CT 06511, USA
    Science 281:272-4. 1998
    ..The Rad9 protein was phosphorylated in response to DNA damage, and phosphorylated Rad9 interacted with the COOH-terminal ..
  36. pmc The Saccharomyces cerevisiae RAD9, RAD17, RAD24 and MEC3 genes are required for tolerating irreparable, ultraviolet-induced DNA damage
    A G Paulovich
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genetics 150:75-93. 1998
    ..We find that RAD9, RAD17, RAD24, and MEC3 are required for UV-induced (although not spontaneous) mutagenesis, and that RAD9 and RAD17 ..
  37. pmc Single-stranded DNA arising at telomeres in cdc13 mutants may constitute a specific signal for the RAD9 checkpoint
    B Garvik
    Department of Genetics, University of Washington, Seattle 98195, USA
    Mol Cell Biol 15:6128-38. 1995
    ..in the G2 phase of the cell cycle at the restrictive temperature as a result of DNA damage that activates the RAD9 checkpoint...
  38. ncbi Budding yeast Rad9 is an ATP-dependent Rad53 activating machine
    C S Gilbert
    Imperial Cancer Research Fund, Clare Hall Laboratories, CDC Laboratory, South Mimms, EN6 3LD, Hertfordshire, United Kingdom
    Mol Cell 8:129-36. 2001
    We find budding yeast Rad9 in two distinct, large, and soluble complexes in cell extracts...
  39. pmc Cdc13 prevents telomere uncapping and Rad50-dependent homologous recombination
    N Grandin
    UMR CNRS ENS no 5665, Ecole Normale Superieure de Lyon, 46, Allee d Italie, 69364 Lyon, France
    EMBO J 20:6127-39. 2001
    ..We propose that Cdc13 prevents telomere uncapping and inhibits recombination between telomeric sequences through a pathway distinct from and complementary to that used by telomerase...
  40. ncbi The BRCT domain of the S. cerevisiae checkpoint protein Rad9 mediates a Rad9-Rad9 interaction after DNA damage
    J Soulier
    Imperial Cancer Research Fund, Clare Hall Laboratories, CDC Laboratory, South Mimms, Hertfordshire, EN6 3LD, UK
    Curr Biol 9:551-4. 1999
    The Saccharomyces cerevisiae checkpoint protein Rad9 is required for transient cell-cycle arrest and transcriptional induction of DNA-repair genes in response to DNA damage [1]...
  41. ncbi MEC1-dependent phosphorylation of Rad9p in response to DNA damage
    A Emili
    Division of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Mol Cell 2:183-9. 1998
    In budding yeast, DNA damage can activate a checkpoint surveillance system controlled by the RAD9, RAD53, and MEC1 genes, resulting in a delay in cell cycle progression...
  42. pmc Histone methyltransferase Dot1 and Rad9 inhibit single-stranded DNA accumulation at DSBs and uncapped telomeres
    Federico Lazzaro
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Milano, Italy
    EMBO J 27:1502-12. 2008
    ..Here, we provide evidence that binding of the checkpoint protein Rad9, through its Tudor domain, to methylated histone H3-K79 inhibits resection at DSBs and uncapped telomeres...
  43. ncbi Histone H2A phosphorylation and H3 methylation are required for a novel Rad9 DSB repair function following checkpoint activation
    Geraldine W L Toh
    Genome Stability Laboratory, Department of Biochemistry and National Centre for Biomedical Engineering Science, National University of Ireland, University Road, Galway, Ireland
    DNA Repair (Amst) 5:693-703. 2006
    In budding yeast, the Rad9 protein is an important player in the maintenance of genomic integrity and has a well-characterised role in DNA damage checkpoint activation...
  44. pmc The role of Stn1p in Saccharomyces cerevisiae telomere capping can be separated from its interaction with Cdc13p
    Ruben C Petreaca
    Cell, Molecular, and Developmental Biology Graduate Program, Department of Cell Biology and Neuroscience, University of California, Riverside, California 92506, USA
    Genetics 177:1459-74. 2007
    ..Thus, an amino-terminal region of Stn1p is sufficient for its essential function, while a central region of Stn1p either negatively regulates the STN1 essential function or destabilizes the mutant Stn1 protein...
  45. ncbi A DNA damage response pathway controlled by Tel1 and the Mre11 complex
    T Usui
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, 560 0043, Osaka, Japan
    Mol Cell 7:1255-66. 2001
    ..In mitotic cells, the Tel1-Mre11 complex pathway triggers Rad53 activation and its interaction with Rad9, whereas in meiosis it acts via Rad9 and the Rad53 paralog Mre4/Mek1...
  46. pmc Replication protein A-mediated recruitment and activation of Rad17 complexes
    Lee Zou
    Brigham and Women s Hospital, Howard Hughes Medical Institute, Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 100:13827-32. 2003
    The human Rad17-Rfc2-5 and Rad9-Rad1-Hus1 complexes play crucial roles in the activation of the ATR-mediated DNA damage and DNA replication stress response pathways...
  47. ncbi Rad9 phosphorylation sites couple Rad53 to the Saccharomyces cerevisiae DNA damage checkpoint
    Marc F Schwartz
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA
    Mol Cell 9:1055-65. 2002
    b>Rad9 is required for the MEC1/TEL1-dependent activation of Saccharomyces cerevisiae DNA damage checkpoint pathways mediated by Rad53 and Chk1...
  48. doi Analysis of replication profiles reveals key role of RFC-Ctf18 in yeast replication stress response
    Laure Crabbe
    Institute of Human Genetics, Centre National de Recherche Scientifique, Unité Propre de Recherche 1142, Montpellier, France
    Nat Struct Mol Biol 17:1391-7. 2010
    ..These data identify RFC(Ctf18) as a key DRC mediator, potentially bridging Mrc1 and primed ssDNA to signal paused forks...
  49. ncbi A domain of Rad9 specifically required for activation of Chk1 in budding yeast
    Richard T Blankley
    School of Biological Sciences, University of Manchester, G38 Stopford Building, Oxford Rd, Manchester, M13 9PT, UK
    J Cell Sci 117:601-8. 2004
    The Rad9 protein is a key adaptor protein in Saccharomyces cerevisiae DNA damage checkpoint pathways...
  50. pmc Yeast pip3/mec3 mutants fail to delay entry into S phase and to slow DNA replication in response to DNA damage, and they define a functional link between Mec3 and DNA primase
    M P Longhese
    Dipartimento di Genetica e di Biologia dei Micorganismi, Universita degli Studi di Milano, Italy
    Mol Cell Biol 16:3235-44. 1996
    ....
  51. pmc The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNA
    Nathalie Lancelot
    Institut de Biologie et Technologies de Saclay, CEA Saclay, 91191 Gif sur Yvette, France
    Nucleic Acids Res 35:5898-912. 2007
    ..Budding yeast Rad9, fission yeast Crb2 and metazoan 53BP1 are presented as mediators involved in the activation of checkpoint kinases...
  52. pmc The SUMO isopeptidase Ulp2p is required to prevent recombination-induced chromosome segregation lethality following DNA replication stress
    Ming Ta Lee
    Department of Cell Biology and Neuroscience, University of California Riverside, Riverside, California, United States of America
    PLoS Genet 7:e1001355. 2011
    ..At the same time, Ulp2p is also required to either suppress or resolve recombination-induced attachments between sister chromatids. These opposing defects may synergize to greatly increase the toxicity of DNA replication stress...
  53. pmc Quantitative proteomic analysis of chromatin reveals that Ctf18 acts in the DNA replication checkpoint
    Takashi Kubota
    Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK
    Mol Cell Proteomics 10:M110.005561. 2011
    ..Identification of Ctf18 as a checkpoint protein highlights the usefulness of chromatin proteomic analysis for understanding the in vivo function of proteins that mediate chromatin transactions...
  54. pmc Pph3-Psy2 is a phosphatase complex required for Rad53 dephosphorylation and replication fork restart during recovery from DNA damage
    Bryan M O'Neill
    Departments of Chemistry and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 104:9290-5. 2007
    ..These findings suggest that Rad53 regulates replication fork restart and initiation of late firing origins independently and that regulation of these processes is mediated by specific Rad53 phosphatases...
  55. pmc Proteins in the nutrient-sensing and DNA damage checkpoint pathways cooperate to restrain mitotic progression following DNA damage
    Jennifer S Searle
    Department of Pharmacology and Toxicology, Norris Cotton Cancer Center, Dartmouth Medical School, Hanover, New Hampshire, USA
    PLoS Genet 7:e1002176. 2011
    ..Our data indicate that there is cross-talk between the DNA damage checkpoint and the proteins that integrate nutrient and stress signals to regulate PKA...
  56. ncbi [Interaction between checkpoint genes RAD9, RAD17, RAD24, and RAD53 involved in the determination of yeast Saccharomyces cerevisiae sensitivity to ionizing radiation]
    N A Koltovaia
    Genetika 44:761-70. 2008
    ..To clarify the role of checkpoint genes RAD9, RAD17, RAD24, and RAD53 in cell radioresistance, double mutants were analyzed for cell sensitivity to ionizing ..
  57. pmc Orchestration of the S-phase and DNA damage checkpoint pathways by replication forks from early origins
    Julie M Caldwell
    Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
    J Cell Biol 180:1073-86. 2008
    ..Thus, oncogene-mediated deregulation of cyclins in the early stages of cancer development could contribute to genomic instability through a deficiency in the forks required to establish the S-phase checkpoint...
  58. ncbi PP2A (Cdc)⁵⁵ is required for multiple events during meiosis I
    Jocelyn K Nolt
    Venenum Biodesign, Genesis Biotechnology Group, Hamilton, NJ, USA
    Cell Cycle 10:1420-34. 2011
    ..Finally, the premeiotic replication defect is suppressed by loss of Rad9 checkpoint function...
  59. pmc The role of replication bypass pathways in dicentric chromosome formation in budding yeast
    Andrew L Paek
    Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USA
    Genetics 186:1161-73. 2010
    ..Fourth, by studying genes implicated in suppression of GCRs in other studies, we found that inverted repeat fusion has a profile of genetic regulation distinct from these other major forms of GCR formation...
  60. pmc RAD9, RAD17, and RAD24 are required for S phase regulation in Saccharomyces cerevisiae in response to DNA damage
    A G Paulovich
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA
    Genetics 145:45-62. 1997
    ..In this report, we show that other genes (RAD9, RAD17, RAD24) involved in the DNA damage checkpoint pathway also play a role in regulating S phase in response to ..
  61. pmc Genome-wide analysis of factors affecting transcription elongation and DNA repair: a new role for PAF and Ccr4-not in transcription-coupled repair
    Hélène Gaillard
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Sevilla, Spain
    PLoS Genet 5:e1000364. 2009
    ....
  62. pmc Contrasting roles of checkpoint proteins as recombination modulators at Fob1-Ter complexes with or without fork arrest
    Bidyut K Mohanty
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    Eukaryot Cell 8:487-95. 2009
    ..Other checkpoint proteins of the checkpoint adapter/mediator class such as Mrc1 and Rad9, which channel signals from the sensor to the effector kinase, tended to suppress recombination at Fob1-Ter ..
  63. pmc Post-replication repair suppresses duplication-mediated genome instability
    Christopher D Putnam
    Ludwig Institute for Cancer Research, University of California San Diego School of Medicine, La Jolla, California, United States of America
    PLoS Genet 6:e1000933. 2010
    ..Our analysis is consistent with models in which PRR prevents replication damage from becoming double strand breaks (DSBs) and/or regulates the activity of HR on DSBs...
  64. pmc Characterization of G1 checkpoint control in the yeast Saccharomyces cerevisiae following exposure to DNA-damaging agents
    W Siede
    Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235
    Genetics 138:271-81. 1994
    ..treatment of yeast cells with DNA-damaging agents is an actively regulated response that requires functional RAD9 and RAD24 genes...
  65. pmc Sua5p a single-stranded telomeric DNA-binding protein facilitates telomere replication
    Fei Long Meng
    The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, China
    EMBO J 28:1466-78. 2009
    ..Thus, Sua5p represents a novel ssTG DNA-binding protein and positively regulates the telomere length in vivo...
  66. pmc Functional connection between the Clb5 cyclin, the protein kinase C pathway and the Swi4 transcription factor in Saccharomyces cerevisiae
    Ethel Queralt
    Departament de Bioquimica i Biologia Molecular, Universitat de Valencia, C Dr Moliner 50, E 46100 Burjassot, Valencia, Spain
    Genetics 171:1485-98. 2005
    ..and Swi4 are involved in the control of DNA integrity: there is a synthetic interaction between pkc1 and slt2 with rad9; the pkc1, slt2, and swi4 mutants are hypersensitive to hydroxyurea; and the Slt2 kinase is activated by ..
  67. pmc Previously uncharacterized genes in the UV- and MMS-induced DNA damage response in yeast
    Denise Hanway
    Department of Chemistry and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 99:10605-10. 2002
    ..Epistatsis analysis of four of the genes was performed to determine the DNA damage repair pathways in which the protein products function...
  68. ncbi Solution structures of two FHA1-phosphothreonine peptide complexes provide insight into the structural basis of the ligand specificity of FHA1 from yeast Rad53
    C Yuan
    Department of Chemistry, The Ohio State University, Columbus OH 43210, USA
    J Mol Biol 314:563-75. 2001
    ..Subsequent location of this motif within the sequence of Rad9, the target protein, coupled with spectroscopic analysis has led to identification of a tight binding sequence that ..
  69. pmc A dominant-negative MEC3 mutant uncovers new functions for the Rad17 complex and Tel1
    Michele Giannattasio
    Dipartimento di Genetica e Biologia dei Microrganismi, Universita degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy
    Proc Natl Acad Sci U S A 99:12997-3002. 2002
    ..We also show that the function of the G(2) checkpoint in mutant cells is maintained by an uncharacterized activity of Tel1, the yeast homologue of ATM. This work thus reports a previously undiscovered role for Tel1 in checkpoint control...
  70. ncbi A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator
    Michael Downey
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON, M5G 1X5, Canada
    Cell 124:1155-68. 2006
    ..Our results therefore indicate that the KEOPS complex promotes both telomere uncapping and telomere elongation...
  71. pmc Suppression of genome instability by redundant S-phase checkpoint pathways in Saccharomyces cerevisiae
    Kyungjae Myung
    Ludwig Institute for Cancer Research, Cancer Center, and Department of Medicine, University of California at San Diego School of Medicine, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 99:4500-7. 2002
    ..These data support the view that spontaneous genome rearrangements result from DNA replication errors and indicate that there is a high degree of redundancy among the checkpoints that act in S phase to suppress such genome instability...
  72. pmc Saccharomyces cerevisiae chromatin-assembly factors that act during DNA replication function in the maintenance of genome stability
    Kyungjae Myung
    Ludwig Institute for Cancer Research, Cancer Center and Department of Medicine, University of California at San Diego School of Medicine, La Jolla 92093, USA
    Proc Natl Acad Sci U S A 100:6640-5. 2003
    ..These results indicate that coupling of chromatin assembly to DNA replication and DNA repair is critical to maintaining genome stability...
  73. pmc In vivo analysis reveals that the interdomain region of the yeast proliferating cell nuclear antigen is important for DNA replication and DNA repair
    N S Amin
    Department of Pharmacology, University of California at San Diego, La Jolla 92093 0651, USA
    Genetics 144:479-93. 1996
    ..At the restrictive temperature, the Cs- pol30 mutants undergo a RAD9-dependent arrest as large-budded cells with a 2c DNA content...
  74. pmc A novel role for the budding yeast RAD9 checkpoint gene in DNA damage-dependent transcription
    A Aboussekhra
    Institut Curie, UMR 144 CNRS, 26 Rue d Ulm, 75231 Paris Cedex 05, France
    EMBO J 15:3912-22. 1996
    ..The G1 and G2 checkpoints have been shown previously to be under the control of the RAD9 gene. We show here that RAD9 is also required for the transcriptional response to DNA damage...
  75. doi The RAD9-dependent gene trans-activation is required for excision repair of active genes but not for repair of non-transcribed DNA
    Nisreen M Al-Moghrabi
    King Faisal Specialist Hospital and Research Center, Department of Biological and Medical Research, Riyadh, Saudi Arabia
    Mutat Res 663:60-8. 2009
    The Saccharomyces cerevisiae RAD9 and RAD24 are two cell cycle checkpoint genes required for UV-dependent up-regulation of a battery of genes involved in different metabolic pathways...
  76. ncbi RAD9, RAD24, RAD16 and RAD26 are required for the inducible nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers from the transcribed and non-transcribed regions of the Saccharomyces cerevisiae MFA2 gene
    S Yu
    School of Biological Sciences, University of Wales Swansea, Singleton Park, SA2 8PP, Swansea, UK
    Mutat Res 485:229-36. 2001
    ..No inducible repair was observed in rad9, rad24, rad16 and rad26 cells, indicating two checkpoint genes RAD9 and RAD24, the global repair gene RAD16 and the ..
  77. pmc Association of Rad9 with double-strand breaks through a Mec1-dependent mechanism
    Takahiro Naiki
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 0814, Japan
    Mol Cell Biol 24:3277-85. 2004
    b>Rad9 is required for the activation of DNA damage checkpoint pathways in budding yeast. Rad9 is phosphorylated after DNA damage in a Mec1- and Tel1-dependent manner and subsequently interacts with Rad53...
  78. doi Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication
    Takashi Ohuchi
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    DNA Repair (Amst) 8:690-6. 2009
    ..Double mutation of RAD51 and RAD53 exhibited the similar levels of Rad52 sumoylation to RAD53 single mutation. The significance and regulation mechanism of Rad52 sumoylation by checkpoint pathways will be discussed...
  79. pmc Yeast Rad52 and Rad51 recombination proteins define a second pathway of DNA damage assessment in response to a single double-strand break
    Sang Eun Lee
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Mol Cell Biol 23:8913-23. 2003
    ..We suggest that monitoring of the extent of DNA damage depends on independent binding of RPA and Rad52p to ssDNA, with Rad52p's activity modulated by Rad51p whereas RPA's action depends on Tid1p...
  80. pmc CDC5 inhibits the hyperphosphorylation of the checkpoint kinase Rad53, leading to checkpoint adaptation
    Genevieve M Vidanes
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, USA
    PLoS Biol 8:e1000286. 2010
    ..Mec1 appeared to be active, since the Rad9 adaptor retained its Mec1 phosphorylation...
  81. doi Mitotic catastrophe induced by overexpression of budding yeast Rad2p
    Mi Sun Kang
    Department of Pharmacology, Inha Research Institute for Medical Sciences, Incheon 400 712, Republic of Korea
    Yeast 27:399-411. 2010
    ..However, Rad2p-induced cell growth arrest is released by rad9 deletion but is not affected by downstream DNA damage checkpoint genes...
  82. doi A postincision-deficient TFIIH causes replication fork breakage and uncovers alternative Rad51- or Pol32-mediated restart mechanisms
    María Moriel-Carretero
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Av Americo Vespucio s n, 41092 Sevilla, Spain
    Mol Cell 37:690-701. 2010
    ..Our results define the genetic and molecular hallmarks of replication fork breakage and restart and bring insights to understand specific NER-related human syndromes...
  83. pmc Rpb1 sumoylation in response to UV radiation or transcriptional impairment in yeast
    Xuefeng Chen
    Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, United States of America
    PLoS ONE 4:e5267. 2009
    ..Our results demonstrate a novel covalent modification of Rpb1 in response to UV induced DNA damage or transcriptional impairment, and unravel an important link between the modification and the DNA damage checkpoint response...
  84. pmc HYS2, an essential gene required for DNA replication in Saccharomyces cerevisiae
    K Sugimoto
    Department of Molecular Biology, Faculty of Science, Nagoya University, Japan
    Nucleic Acids Res 23:3493-500. 1995
    ..Finally, deletion of RAD9 in a hys2-1 strain decreases the percentage of arrested cells, suggesting that an intact RAD9-checkpoint is ..
  85. ncbi Pathways for repair of topoisomerase I covalent complexes in Saccharomyces cerevisiae
    J J Pouliot
    Laboratory of Molecular Biology, National Institute of Mental Health, Building 36, Room 1B08, Bethesda, MD 20892 4034, USA
    Genes Cells 6:677-87. 2001
    ..Tdp1, an enzyme that can hydrolyse the bond between topoisomerase I and DNA, is thought to be involved in the repair of these lesions, but little is known about how such repair is accomplished...
  86. ncbi Nuclear oscillations and nuclear filament formation accompany single-strand annealing repair of a dicentric chromosome in Saccharomyces cerevisiae
    Douglas A Thrower
    Department of Biology, CB3280 University of North Carolina, Chapel Hill, NC 27599 3280, USA
    J Cell Sci 116:561-9. 2003
    ..repair requires the adaptation-defective cdc5-ad allele of the yeast polo kinase and the DNA damage checkpoint gene RAD9. Dicentric chromosome breakage in cdc5-ad rad52 mutant cells is associated with a prolonged mitotic arrest, during ..
  87. pmc Telomerase subunit overexpression suppresses telomere-specific checkpoint activation in the yeast yku80 mutant
    S H Teo
    Wellcome Trust and Cancer Research Campaign, Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge CB2 1QR, UK
    EMBO Rep 2:197-202. 2001
    ....
  88. pmc Yeast telomere capping protein Stn1 overrides DNA replication control through the S phase checkpoint
    Hovik J Gasparyan
    Department of Cell Biology and Neuroscience, University of California, Biological Sciences Building, Riverside, CA 92521, USA
    Proc Natl Acad Sci U S A 106:2206-11. 2009
    ..During replication stress, the mislocalized Stn1 may inappropriately promote Pol alpha in a manner that interferes with Rad53 effector mechanisms controlling replication fork integrity...
  89. ncbi Mutation of Gly721 alters DNA topoisomerase I active site architecture and sensitivity to camptothecin
    Marie van der Merwe
    Department of Molecular Pharmacology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 283:3305-15. 2008
    ..We postulate that this conserved Gly residue provides a flexible hinge within the Top1p catalytic pocket to facilitate linker dynamics and the structural alterations that accompany drug binding of the covalent enzyme-DNA intermediate...
  90. ncbi Hst3 is regulated by Mec1-dependent proteolysis and controls the S phase checkpoint and sister chromatid cohesion by deacetylating histone H3 at lysine 56
    Safia Thaminy
    Clinical Research Division and Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    J Biol Chem 282:37805-14. 2007
    ..The pathway that regulates H3K56 acetylation acts in parallel with the Rad9 pathway to transmit a DNA damage signal from Mec1 to Rad53...
  91. pmc Association with the origin recognition complex suggests a novel role for histone acetyltransferase Hat1p/Hat2p
    Bernhard Suter
    Program in Proteomics and Bioinformatics, Banting and Best Department of Medical Genetics, University of Toronto, Toronto, Ontario, Canada
    BMC Biol 5:38. 2007
    ..In yeast, a major conserved histone acetyltransferase, Hat1p, preferentially acetylates lysine residues 5 and 12 on histone H4...
  92. pmc Control of translocations between highly diverged genes by Sgs1, the Saccharomyces cerevisiae homolog of the Bloom's syndrome protein
    Kristina H Schmidt
    Department of Biology, University of South Florida, 4202 E Fowler Avenue, SCA110, Tampa, FL 33620, USA
    Mol Cell Biol 26:5406-20. 2006
    ..between diverged genes in cells with mutations in the genes encoding the checkpoint proteins Mec3, Rad24, Rad9, or Rfc5, the chromatin assembly factors Cac1 or Asf1, and the DNA helicase Rrm3...
  93. ncbi Regulation of the Rad53 protein kinase in signal amplification by oligomer assembly and disassembly
    Nianhan Jia-Lin Ma
    Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Cell Cycle 7:808-17. 2008
    ..The mediator Rad9 is not necessary for Rad53 oligomerization...
  94. ncbi Uncoupling of unwinding from DNA synthesis implies regulation of MCM helicase by Tof1/Mrc1/Csm3 checkpoint complex
    Marina N Nedelcheva
    Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria
    J Mol Biol 347:509-21. 2005
    ..In concordance with this suggestion, we found that the Tof1/Csm3/Mrc1 checkpoint complex interacts directly with the MCM helicase during both replication fork progression and when the replication fork is stalled...
  95. pmc Genetic analysis of Saccharomyces cerevisiae H2A serine 129 mutant suggests a functional relationship between H2A and the sister-chromatid cohesion partners Csm3-Tof1 for the repair of topoisomerase I-induced DNA damage
    Christophe Redon
    NIH, NCI, DBS, Laboratory of Molecular Pharmacology, Bethesda, Maryland 20892, USA
    Genetics 172:67-76. 2006
    ..the pathway involving H2A/Csm3/Tof1 is distinct from the pathways involving the Ctf8/Ctf18/Dcc1 complex, the Rad9 pathway, and another involving Mrc1...
  96. pmc Mutants defective in Rad1-Rad10-Slx4 exhibit a unique pattern of viability during mating-type switching in Saccharomyces cerevisiae
    Amy M Lyndaker
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853 2703, USA
    Genetics 179:1807-21. 2008
    ..We found that mutants lacking an intact Rad1-Rad10-Slx4 complex displayed RAD9- and MAD2-dependent cell cycle delays and decreased viability during mating-type switching...
  97. pmc Mechanisms of cell cycle control revealed by a systematic and quantitative overexpression screen in S. cerevisiae
    Wei Niu
    Center for Systems and Synthetic Biology, University of Texas, Austin, Texas, United States of America
    PLoS Genet 4:e1000120. 2008
    ..This work thus implicates new genes in cell cycle progression, complements previous screens, and lays the foundation for future experiments to define more precisely roles for these genes in cell cycle progression...
  98. pmc The RAD6/BRE1 histone modification pathway in Saccharomyces confers radiation resistance through a RAD51-dependent process that is independent of RAD18
    John C Game
    Life Sciences Division, Lawrence Berkeley National Laboratory, CA 94720, USA
    Genetics 173:1951-68. 2006
    ..We conclude that IR resistance conferred by BRE1 and DOT1 is mediated through homologous recombinational repair, not postreplication repair, and confirm findings of a G1 checkpoint role for the RAD6/BRE1/DOT1 pathway...
  99. ncbi The Saccharomyces cerevisiae checkpoint genes RAD9, CHK1 and PDS1 are required for elevated homologous recombination in a mec1 (ATR) hypomorphic mutant
    Michael Fasullo
    Ordway Research Institute, Albany, New York 12208, USA
    Cell Cycle 7:2418-26. 2008
    ..The mec1-21 hyper-recombination was partially reduced in rad9, pds1 and chk1 mutants, and abolished in rad52 mutants, suggesting the hyper-recombination results from RAD52-..
  100. pmc Reconstitution of Rad53 activation by Mec1 through adaptor protein Mrc1
    Sheng Hong Chen
    Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093 0653, USA
    J Biol Chem 284:18593-604. 2009
    ..Further, the conserved C-terminal domain of Mrc1 was found to be required for Rad53 activation. These results thus provide insights into the role of the adaptor protein Mrc1 in activating Rad53 in the DNA replication checkpoint...
  101. ncbi Budding yeast 14-3-3 proteins contribute to the robustness of the DNA damage and spindle checkpoints
    Nathalie Grandin
    IFR128 BioSciences Gerland, UMR CNRS ENS n degrees 5239, Ecole Normale Superieure de Lyon, Lyon, France
    Cell Cycle 7:2749-61. 2008
    ..Therefore, the budding yeast 14-3-3 proteins contribute to the robustness of the two major mitotic checkpoints and, by doing so, may also ensure optimal coordination between the responses to two distinct types of damage...

Research Grants18

  1. Suppression of translocations by RecQ-like DNA helicases
    KRISTINA HILDEGARD SCHMIDT; Fiscal Year: 2010
    ....
  2. Activating liver carcinogens in yeast by expressing CYP450 polymorphisms
    Michael Fasullo; Fiscal Year: 2007
    ..Ultimately, the information will aid public health practitioners and clinicians to identify which individuals are at highest risk for liver cancer, and guide prevention and early detection efforts. ..
  3. Activating liver carcinogens in yeast by expressing CYP450 polymorphisms
    Michael Fasullo; Fiscal Year: 2007
    ....
  4. Repair Pathways for Ionizing Radiation Damage Yeast
    John Game; Fiscal Year: 2006
    ..Recent and existing mutants will be studied in detail with this system to determine the molecular involvement of each gene in DSB repair, sister chromatid exchange and recombination between chromosomes. ..
  5. RADIATION INDUCTION OF GENOMIC REARRANGEMENTS IN YEAST
    Michael Fasullo; Fiscal Year: 2004
    ..The purpose of this proposal is to elucidate the genetic pathway by which the Saccharomyces cerevisiae RAD9 checkpoint reduces genetic instability that results from homologous recombination between repeated sequences (..
  6. REPAIR PATHWAYS FOR IONIZING RADIATION DAMAGE IN YEAST
    John Game; Fiscal Year: 2002
    ..In a related aim, the order of gene function for RAD5O and RAD54 will be tested using different switching regimes with double mutants containing conditional alleles with differing permissive conditions. ..
  7. Suppression of translocations by RecQ-like DNA helicases
    KRISTINA HILDEGARD SCHMIDT; Fiscal Year: 2010
    ....