Gene Symbol: RAD54
Description: DNA-dependent ATPase RAD54
Alias: XRS1, DNA-dependent ATPase RAD54
Species: Saccharomyces cerevisiae S288c
Products:     RAD54

Top Publications

  1. Seong C, Colavito S, Kwon Y, Sung P, Krejci L. Regulation of Rad51 recombinase presynaptic filament assembly via interactions with the Rad52 mediator and the Srs2 anti-recombinase. J Biol Chem. 2009;284:24363-71 pubmed publisher
    ..Moreover, our biochemical studies have helped identify A320V as a separation-of-function mutation in Rad51 with regards to a differential ablation of Rad52 interaction. ..
  2. Petukhova G, Van Komen S, Vergano S, Klein H, Sung P. Yeast Rad54 promotes Rad51-dependent homologous DNA pairing via ATP hydrolysis-driven change in DNA double helix conformation. J Biol Chem. 1999;274:29453-62 pubmed
    Saccharomyces cerevisiae RAD54 gene functions in the formation of heteroduplex DNA, a key intermediate in recombination processes. Rad54 is monomeric in solution, but forms a dimer/oligomer on DNA...
  3. Ball L, Zhang K, Cobb J, Boone C, Xiao W. The yeast Shu complex couples error-free post-replication repair to homologous recombination. Mol Microbiol. 2009;73:89-102 pubmed publisher
    ..This mechanism appears to be conserved throughout eukaryotes. ..
  4. Clever B, Interthal H, Schmuckli Maurer J, King J, Sigrist M, Heyer W. Recombinational repair in yeast: functional interactions between Rad51 and Rad54 proteins. EMBO J. 1997;16:2535-44 pubmed
    ..Here we demonstrate by genetic, molecular and biochemical criteria that Rad51 and Rad54 proteins interact...
  5. Gangavarapu V, Prakash S, Prakash L. Requirement of RAD52 group genes for postreplication repair of UV-damaged DNA in Saccharomyces cerevisiae. Mol Cell Biol. 2007;27:7758-64 pubmed
    ..Here, we examine the contributions of the RAD51, RAD52, and RAD54 genes and of the RAD50 and XRS2 genes to the PRR of UV-damaged DNA...
  6. Shor E, Gangloff S, Wagner M, Weinstein J, Price G, Rothstein R. Mutations in homologous recombination genes rescue top3 slow growth in Saccharomyces cerevisiae. Genetics. 2002;162:647-62 pubmed
    ..We found that slow growth and DNA damage sensitivity of top3 mutants are suppressed by mutations in RAD51, RAD54, RAD55, and RAD57...
  7. Petukhova G, Stratton S, Sung P. Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins. Nature. 1998;393:91-4 pubmed
    The Saccharomyces cerevisiae RAD51 and RAD54 genes are both required for the occurrence of homologous recombination and for the repair of double-stranded DNA breaks...
  8. Smirnova M, Van Komen S, Sung P, Klein H. Effects of tumor-associated mutations on Rad54 functions. J Biol Chem. 2004;279:24081-8 pubmed
    Yeast RAD54 gene, a member of the RAD52 epistasis group, plays an important role in homologous recombination and DNA double strand break repair...
  9. Mazin A, Alexeev A, Kowalczykowski S. A novel function of Rad54 protein. Stabilization of the Rad51 nucleoprotein filament. J Biol Chem. 2003;278:14029-36 pubmed
    Homologous recombination is important for the repair of double-stranded DNA breaks in all organisms. Rad51 and Rad54 proteins are two key components of the homologous recombination machinery in eukaryotes...

More Information


  1. Shinohara M, Shita Yamaguchi E, Buerstedde J, Shinagawa H, Ogawa H, Shinohara A. Characterization of the roles of the Saccharomyces cerevisiae RAD54 gene and a homologue of RAD54, RDH54/TID1, in mitosis and meiosis. Genetics. 1997;147:1545-56 pubmed
    The RAD54 gene, which encodes a protein in the SWI2/SNF2 family, plays an important role in recombination and DNA repair in Saccharomyces cerevisiae. The yeast genome project revealed a homologue of RAD54, RDH54/TID1...
  2. Krejci L, Damborsky J, Thomsen B, Duno M, Bendixen C. Molecular dissection of interactions between Rad51 and members of the recombination-repair group. Mol Cell Biol. 2001;21:966-76 pubmed
    ..The Rad51 protein associates with the Rad51, Rad52, Rad54, and Rad55 proteins to form a dynamic complex...
  3. Pâques F, Haber J. Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae. Microbiol Mol Biol Rev. 1999;63:349-404 pubmed
    ..This review encompasses these different aspects of DSB-induced recombination in Saccharomyces and attempts to relate genetic, molecular biological, and biochemical studies of the processes of DNA repair and recombination. ..
  4. Morgan E, Shah N, Symington L. The requirement for ATP hydrolysis by Saccharomyces cerevisiae Rad51 is bypassed by mating-type heterozygosity or RAD54 in high copy. Mol Cell Biol. 2002;22:6336-43 pubmed
    ..b>RAD54 expressed from a high-copy-number plasmid also suppressed the gamma-ray sensitivity of rad51-K191R haploids...
  5. Sabourin M, Nitiss J, Nitiss K, Tatebayashi K, Ikeda H, Osheroff N. Yeast recombination pathways triggered by topoisomerase II-mediated DNA breaks. Nucleic Acids Res. 2003;31:4373-84 pubmed
    ..Non-homologous end joining also was triggered by etoposide treatment, but this pathway was considerably less active than single-strand invasion and did not contribute significantly to cell survival in S.cerevisiae. ..
  6. Interthal H, Heyer W. MUS81 encodes a novel helix-hairpin-helix protein involved in the response to UV- and methylation-induced DNA damage in Saccharomyces cerevisiae. Mol Gen Genet. 2000;263:812-27 pubmed
    ..UV sensitive) was identified as clone 81 in a two-hybrid screen using the Saccharomyces cerevisiae Rad54 protein as a bait...
  7. Krejci L, Macris M, Li Y, Van Komen S, Villemain J, Ellenberger T, et al. Role of ATP hydrolysis in the antirecombinase function of Saccharomyces cerevisiae Srs2 protein. J Biol Chem. 2004;279:23193-9 pubmed
    ..These results highlight the importance of the ATP hydrolysisfueled DNA motor activity in SRS2 functions. ..
  8. Raschle M, Van Komen S, Chi P, Ellenberger T, Sung P. Multiple interactions with the Rad51 recombinase govern the homologous recombination function of Rad54. J Biol Chem. 2004;279:51973-80 pubmed
    In eukaryotes, Rad51 and Rad54 functionally cooperate to mediate homologous recombination and the repair of damaged chromosomes by recombination...
  9. Shah P, Zheng X, Epshtein A, Carey J, Bishop D, Klein H. Swi2/Snf2-related translocases prevent accumulation of toxic Rad51 complexes during mitotic growth. Mol Cell. 2010;39:862-72 pubmed publisher
    Purified DNA translocases Rdh54 and Rad54 can dissociate complexes formed by eukaryotic RecA-like recombinases on double-stranded DNA. Here, we show that Rad51 complexes are dissociated by these translocases in mitotic cells...
  10. Rattray A, Symington L. Multiple pathways for homologous recombination in Saccharomyces cerevisiae. Genetics. 1995;139:45-56 pubmed
    ..The majority of recombination events are mediated by a RAD51-dependent pathway, where the RAD54, RAD55 and RAD57 genes function downstream of RAD51...
  11. Li X, Heyer W. RAD54 controls access to the invading 3'-OH end after RAD51-mediated DNA strand invasion in homologous recombination in Saccharomyces cerevisiae. Nucleic Acids Res. 2009;37:638-46 pubmed publisher
    ..Here we show that, the Saccharomyces cerevisiae dsDNA motor protein Rad54 solves this problem by dissociating yeast Rad51 protein bound to the heteroduplex DNA after DNA strand invasion...
  12. Alexeev A, Mazin A, Kowalczykowski S. Rad54 protein possesses chromatin-remodeling activity stimulated by the Rad51-ssDNA nucleoprotein filament. Nat Struct Biol. 2003;10:182-6 pubmed
    In Saccharomyces cerevisiae, the Rad54 protein participates in the recombinational repair of double-strand DNA breaks together with the Rad51, Rad52, Rad55 and Rad57 proteins...
  13. Burkovics P, Sebesta M, Sisakova A, Plault N, Szukacsov V, Robert T, et al. Srs2 mediates PCNA-SUMO-dependent inhibition of DNA repair synthesis. EMBO J. 2013;32:742-55 pubmed publisher
    ..Our results suggest that cycling cells use the Siz1-dependent SUMOylation of PCNA to limit the extension of repair synthesis during template switch or HR and attenuate reciprocal DNA strand exchanges to maintain genome stability. ..
  14. Jiang H, Xie Y, Houston P, Stemke Hale K, Mortensen U, Rothstein R, et al. Direct association between the yeast Rad51 and Rad54 recombination proteins. J Biol Chem. 1996;271:33181-6 pubmed
    The RAD54 and RAD51 genes are involved in genetic recombination and double-strand break repair in the yeast Saccharomyces cerevisiae...
  15. Klein H. Mutations in recombinational repair and in checkpoint control genes suppress the lethal combination of srs2Delta with other DNA repair genes in Saccharomyces cerevisiae. Genetics. 2001;157:557-65 pubmed
    ..rescued by mutations in the DNA damage checkpoint functions RAD9, RAD17, RAD24, and MEC3, indicating that the srs2 rad54 and srs2 rdh54 mutant combinations lead to an intermediate that is sensed by these checkpoint functions...
  16. Klein H. RDH54, a RAD54 homologue in Saccharomyces cerevisiae, is required for mitotic diploid-specific recombination and repair and for meiosis. Genetics. 1997;147:1533-43 pubmed
    ..The RDH54 gene is homologous to the RAD54 gene, but rdh54 mutants do not show sensitivity to methyl methanesulfonate at concentrations that sensitize a ..
  17. Dresser M, Ewing D, Conrad M, Dominguez A, Barstead R, Jiang H, et al. DMC1 functions in a Saccharomyces cerevisiae meiotic pathway that is largely independent of the RAD51 pathway. Genetics. 1997;147:533-44 pubmed
    ..Interestingly, the pattern on CHEF gels of chromosome fragments that result from meiotic DNA double-strand break formation is different in DMC1 mutant strains from that seen in rad50S strains. ..
  18. Busygina V, Sehorn M, Shi I, Tsubouchi H, Roeder G, Sung P. Hed1 regulates Rad51-mediated recombination via a novel mechanism. Genes Dev. 2008;22:786-95 pubmed publisher
    ..affect assembly of the Rad51 presynaptic filament, but it specifically prohibits interaction of Rad51 with Rad54, a Swi2/Snf2-like factor that is indispensable for Rad51-mediated recombination...
  19. Liu J, Ede C, Wright W, Gore S, Jenkins S, Freudenthal B, et al. Srs2 promotes synthesis-dependent strand annealing by disrupting DNA polymerase δ-extending D-loops. elife. 2017;6: pubmed publisher
    ..Specifically, we partly reconstitute the SDSA pathway using Rad51, Rad54, RPA, RFC, DNA Polymerase δ with different forms of PCNA...
  20. Ii M, Ii T, Mironova L, Brill S. Epistasis analysis between homologous recombination genes in Saccharomyces cerevisiae identifies multiple repair pathways for Sgs1, Mus81-Mms4 and RNase H2. Mutat Res. 2011;714:33-43 pubmed publisher
    ..In contrast, RAD54, RAD55 and RAD57 are epistatic to SGS1, MUS81 and RNH202...
  21. Bishop D, Nikolski Y, Oshiro J, Chon J, Shinohara M, Chen X. High copy number suppression of the meiotic arrest caused by a dmc1 mutation: REC114 imposes an early recombination block and RAD54 promotes a DMC1-independent DSB repair pathway. Genes Cells. 1999;4:425-44 pubmed
    ..High copy numbers of RAD54 suppress dmc1 arrest, promote DSB repair, and allow formation of viable spores following reductional segregation...
  22. Kim D, Harris B, Wang F, Seidel C, McCroskey S, Gerton J. Mms21 SUMO Ligase Activity Promotes Nucleolar Function in Saccharomyces cerevisiae. Genetics. 2016;204:645-658 pubmed
    ..Deletion of a previously identified strong suppressor, MPH1, rescues both the accumulation of ribosome subunits and translational stress. This study suggests that the Smc5/6 complex supports nucleolar function. ..
  23. Schmidt K, Kolodner R. Requirement of Rrm3 helicase for repair of spontaneous DNA lesions in cells lacking Srs2 or Sgs1 helicase. Mol Cell Biol. 2004;24:3213-26 pubmed
    ..These observations identify Rrm3 as a new member of a network of pathways, involving Sgs1 and Srs2 helicases and Mus81 endonuclease, suggested to act during repair of stalled replication forks. ..
  24. Busygina V, Saro D, Williams G, Leung W, Say A, Sehorn M, et al. Novel attributes of Hed1 affect dynamics and activity of the Rad51 presynaptic filament during meiotic recombination. J Biol Chem. 2012;287:1566-75 pubmed publisher
    ..a meiosis-specific protein in Saccharomyces cerevisiae, restricts the access of the recombinase accessory factor Rad54 to presynaptic filaments of Rad51...
  25. Shinohara M, Sakai K, Shinohara A, Bishop D. Crossover interference in Saccharomyces cerevisiae requires a TID1/RDH54- and DMC1-dependent pathway. Genetics. 2003;163:1273-86 pubmed
    ..Two partially redundant proteins, Rad54 and Tid1/Rdh54, act as recombinase accessory factors...
  26. Callender T, Laureau R, Wan L, Chen X, Sandhu R, Laljee S, et al. Mek1 Down Regulates Rad51 Activity during Yeast Meiosis by Phosphorylation of Hed1. PLoS Genet. 2016;12:e1006226 pubmed publisher
    ..Mek1 and occurs through two different mechanisms that prevent complex formation with the Rad51 accessory factor Rad54: (i) phosphorylation of Rad54 by Mek1 and (ii) binding of Rad51 by the meiosis-specific protein Hed1...
  27. Erlich R, Fry R, Begley T, Daee D, Lahue R, Samson L. Anc1, a protein associated with multiple transcription complexes, is involved in postreplication repair pathway in S. cerevisiae. PLoS ONE. 2008;3:e3717 pubmed publisher
    ..Anc1's role in the PRR pathway, as well as its role in suppressing triplet repeats, point to a possible mechanism for a protein of potential medical interest. ..
  28. Wagner M, Price G, Rothstein R. The absence of Top3 reveals an interaction between the Sgs1 and Pif1 DNA helicases in Saccharomyces cerevisiae. Genetics. 2006;174:555-73 pubmed
    ..Our results support a model in which Pif1 has a direct role in the prevention or repair of Sgs1-induced DNA damage that accumulates in top3 mutants. ..
  29. Moriel Carretero M, Aguilera A. A postincision-deficient TFIIH causes replication fork breakage and uncovers alternative Rad51- or Pol32-mediated restart mechanisms. Mol Cell. 2010;37:690-701 pubmed publisher
    ..Our results define the genetic and molecular hallmarks of replication fork breakage and restart and bring insights to understand specific NER-related human syndromes. ..
  30. Shinohara M, Sakai K, Ogawa T, Shinohara A. The mitotic DNA damage checkpoint proteins Rad17 and Rad24 are required for repair of double-strand breaks during meiosis in yeast. Genetics. 2003;164:855-65 pubmed
    ..Consistent with this proposal, extra copies of RAD51 and RAD54 substantially suppress not only the spore inviability of the rad24 mutant, but also the gamma-ray sensitivity of ..
  31. Arbel Eden A, Joseph Strauss D, Masika H, Printzental O, Rachi E, Simchen G. Trans-Lesion DNA Polymerases May Be Involved in Yeast Meiosis. G3 (Bethesda). 2013;3:633-644 pubmed publisher
  32. Mimura S, Yamaguchi T, Ishii S, Noro E, Katsura T, Obuse C, et al. Cul8/Rtt101 forms a variety of protein complexes that regulate DNA damage response and transcriptional silencing. J Biol Chem. 2010;285:9858-67 pubmed publisher
    ..These results suggest that Cul8-Mms1, as part of various protein complexes, is involved in the regulation of chromatin metabolism. ..
  33. Kniewel R, Murakami H, Liu Y, Ito M, Ohta K, Hollingsworth N, et al. Histone H3 Threonine 11 Phosphorylation Is Catalyzed Directly by the Meiosis-Specific Kinase Mek1 and Provides a Molecular Readout of Mek1 Activity in Vivo. Genetics. 2017;207:1313-1333 pubmed publisher
    ..These findings provide insight into the spatial disposition of Mek1 kinase activity and the higher order organization of recombining meiotic chromosomes. ..
  34. Wright W, Heyer W. Rad54 functions as a heteroduplex DNA pump modulated by its DNA substrates and Rad51 during D loop formation. Mol Cell. 2014;53:420-32 pubmed publisher
    ..In eukaryotes, the Rad51 DNA strand exchange protein is assisted in D loop formation by the Rad54 motor protein. Curiously, Rad54 also disrupts D loops...
  35. Xiong L, Chen X, Silver H, Ahmed N, Johnson E. Deficient SUMO attachment to Flp recombinase leads to homologous recombination-dependent hyperamplification of the yeast 2 microm circle plasmid. Mol Biol Cell. 2009;20:1241-51 pubmed publisher
    ..This work also illustrates the importance of using cir(o) strains when studying mutants that affect the yeast SUMO pathway, to avoid confusing direct functions of the SUMO pathway with secondary effects of 2 microm amplification. ..
  36. Glassner B, Mortimer R. Synergistic interactions between RAD5, RAD16 and RAD54, three partially homologous yeast DNA repair genes each in a different repair pathway. Radiat Res. 1994;139:24-33 pubmed
    Considerable homology has recently been noted between the proteins encoded by the RAD5, RAD16 and RAD54 genes of Saccharomyces cerevisiae...
  37. Symington L. Homologous recombination is required for the viability of rad27 mutants. Nucleic Acids Res. 1998;26:5589-95 pubmed
    ..strain containing a null allele of RAD27 to strains containing a mutation in either the RAD1, RAD50, RAD51, RAD52, RAD54, RAD55, RAD57, MRE11, XRS2 or RAD59 gene...
  38. Myung K, Pennaneach V, Kats E, Kolodner R. Saccharomyces cerevisiae chromatin-assembly factors that act during DNA replication function in the maintenance of genome stability. Proc Natl Acad Sci U S A. 2003;100:6640-5 pubmed
    ..These results indicate that coupling of chromatin assembly to DNA replication and DNA repair is critical to maintaining genome stability. ..
  39. Chen Q, Ijpma A, Greider C. Two survivor pathways that allow growth in the absence of telomerase are generated by distinct telomere recombination events. Mol Cell Biol. 2001;21:1819-27 pubmed
    ..Mutations in RAD51, RAD54, and RAD57 had the converse affect: they eliminated the ability of cells to generate type I survivors in a tlc1 ..
  40. Usui T, Kanehara Y. Elevated Rad53 kinase activity influences formation and interhomolog repair of meiotic DNA double-strand breaks in budding yeast. Biochem Biophys Res Commun. 2013;441:593-9 pubmed publisher
    ..The overexpression led to partial activation of Rad53, uncovering that the negative impacts of Rad53 kinase activation on meiotic progression, and formation and interhomolog repair of meiotic programmed DSBs. ..
  41. Soustelle C, Vernis L, Fréon K, Reynaud Angelin A, Chanet R, Fabre F, et al. A new Saccharomyces cerevisiae strain with a mutant Smt3-deconjugating Ulp1 protein is affected in DNA replication and requires Srs2 and homologous recombination for its viability. Mol Cell Biol. 2004;24:5130-43 pubmed
    ..These structures are believed to generate different recombination intermediates. Some of them are fixed by recombination, and others require Srs2 to be reversed and fixed by an alternate pathway. ..
  42. Matulova P, Marini V, Burgess R, Sisakova A, Kwon Y, Rothstein R, et al. Cooperativity of Mus81.Mms4 with Rad54 in the resolution of recombination and replication intermediates. J Biol Chem. 2009;284:7733-45 pubmed publisher
    ..Previous yeast two-hybrid studies have found an interaction of the Mus81 protein with Rad54, a Swi2/Snf2-like factor that serves multiple roles in homologous recombination processes...
  43. Tsang C, Zheng X. Opposing role of condensin and radiation-sensitive gene RAD52 in ribosomal DNA stability regulation. J Biol Chem. 2009;284:21908-19 pubmed publisher
    ..In contrast, deletion of RAD52 restores cell viability under the same conditions. These results reveal an opposing role of condensin and Rad52 in the control of rDNA stability under nutrient starvation conditions. ..
  44. Herruzo E, Ontoso D, González Arranz S, Cavero S, Lechuga A, San Segundo P. The Pch2 AAA+ ATPase promotes phosphorylation of the Hop1 meiotic checkpoint adaptor in response to synaptonemal complex defects. Nucleic Acids Res. 2016;44:7722-41 pubmed publisher
    ..Based on our results, we propose that, under checkpoint-inducing conditions, Pch2 also possesses a positive action on Hop1 promoting its phosphorylation and its proper distribution on unsynapsed chromosome axes. ..
  45. Baldwin E, Berger A, Corbett A, Osheroff N. Mms22p protects Saccharomyces cerevisiae from DNA damage induced by topoisomerase II. Nucleic Acids Res. 2005;33:1021-30 pubmed
    ..Finally, consistent with a role in DNA processes, Mms22p localizes to discrete nuclear foci, even in the absence of etoposide or its binding partners. ..
  46. Chi P, Kwon Y, Visnapuu M, Lam I, Santa Maria S, Zheng X, et al. Analyses of the yeast Rad51 recombinase A265V mutant reveal different in vivo roles of Swi2-like factors. Nucleic Acids Res. 2011;39:6511-22 pubmed publisher
    The Saccharomyces cerevisiae Swi2-like factors Rad54 and Rdh54 play multifaceted roles in homologous recombination via their DNA translocase activity...
  47. Chen X, Cui D, Papusha A, Zhang X, Chu C, Tang J, et al. The Fun30 nucleosome remodeller promotes resection of DNA double-strand break ends. Nature. 2012;489:576-80 pubmed publisher
    ..Together these data suggest that Fun30 helps to overcome the inhibitory effect of Rad9 on DNA resection. ..
  48. Kwon Y, Chi P, Roh D, Klein H, Sung P. Synergistic action of the Saccharomyces cerevisiae homologous recombination factors Rad54 and Rad51 in chromatin remodeling. DNA Repair (Amst). 2007;6:1496-506 pubmed
    b>Rad54, a member of the Swi2/Snf2 protein family, works in concert with the RecA-like recombinase Rad51 during the early and late stages of homologous recombination...
  49. Chen X, Suhandynata R, Sandhu R, Rockmill B, Mohibullah N, Niu H, et al. Phosphorylation of the Synaptonemal Complex Protein Zip1 Regulates the Crossover/Noncrossover Decision during Yeast Meiosis. PLoS Biol. 2015;13:e1002329 pubmed publisher
    ..These results suggest that phosphorylation of Zip1 at DSBs commits those breaks to repair via the ZMM pathway and provides a mechanism by which the crossover/noncrossover decision can be dynamically regulated during yeast meiosis. ..
  50. Panico E, Ede C, Schildmann M, Schürer K, Kramer W. Genetic evidence for a role of Saccharomyces cerevisiae Mph1 in recombinational DNA repair under replicative stress. Yeast. 2010;27:11-27 pubmed publisher
    ..Importantly, rad54 and mph1, epistatic for damage sensitivity, are subadditive for spontaneous mutator phenotype...
  51. Esta A, Ma E, Dupaigne P, Maloisel L, Guerois R, Le Cam E, et al. Rad52 sumoylation prevents the toxicity of unproductive Rad51 filaments independently of the anti-recombinase Srs2. PLoS Genet. 2013;9:e1003833 pubmed publisher
    ..This conclusion is strengthened by the finding that Rad52 is often associated with complete Rad51 filaments in vitro...
  52. Klutstein M, Shaked H, Sherman A, Avivi Ragolsky N, Shema E, Zenvirth D, et al. Functional conservation of the yeast and Arabidopsis RAD54-like genes. Genetics. 2008;178:2389-97 pubmed publisher
    The Saccharomyces cerevisiae RAD54 gene has critical roles in DNA double-strand break repair, homologous recombination, and gene targeting...
  53. Kaliappan K, Choudhury N, Suyal G, Mukherjee S. A novel role for RAD54: this host protein modulates geminiviral DNA replication. FASEB J. 2012;26:1142-60 pubmed publisher
    ..We have identified a host factor, RAD54, in a screen for Rep-interacting partners and observed its role in DNA replication of the geminivirus mungbean ..
  54. Conde F, San Segundo P. Role of Dot1 in the response to alkylating DNA damage in Saccharomyces cerevisiae: regulation of DNA damage tolerance by the error-prone polymerases Polzeta/Rev1. Genetics. 2008;179:1197-210 pubmed publisher
    ..Deletion of DOT1 partially or totally suppresses the MMS sensitivity of various DNA repair mutants (rad52, rad54, yku80, rad1, rad14, apn1, rad5, rad30)...
  55. Chin J, Bashkirov V, Heyer W, Romesberg F. Esc4/Rtt107 and the control of recombination during replication. DNA Repair (Amst). 2006;5:618-28 pubmed
    ..Thus, we propose that Esc4 associates with ssDNA of stalled forks and acts as a scaffolding protein to recruit and/or modulate the function of other proteins required to reinitiate DNA synthesis. ..
  56. Najor N, Weatherford L, Brush G. Prevention of DNA Rereplication Through a Meiotic Recombination Checkpoint Response. G3 (Bethesda). 2016;6:3869-3881 pubmed publisher
    ..While this response is similar to a checkpoint mechanism that inhibits initiation of DNA replication in the mitotic cell cycle, the evidence points to a new variation on DNA replication control. ..
  57. Solinger J, Lutz G, Sugiyama T, Kowalczykowski S, Heyer W. Rad54 protein stimulates heteroduplex DNA formation in the synaptic phase of DNA strand exchange via specific interactions with the presynaptic Rad51 nucleoprotein filament. J Mol Biol. 2001;307:1207-21 pubmed
    b>RAD54 is an important member of the RAD52 group of genes that carry out recombinational repair of DNA damage in the yeast Saccharomyces cerevisiae...
  58. Halmai M, Frittmann O, Szabo Z, Daraba A, Gali V, Balint E, et al. Mutations at the Subunit Interface of Yeast Proliferating Cell Nuclear Antigen Reveal a Versatile Regulatory Domain. PLoS ONE. 2016;11:e0161307 pubmed publisher
  59. Refolio E, Cavero S, Marcon E, Freire R, San Segundo P. The Ddc2/ATRIP checkpoint protein monitors meiotic recombination intermediates. J Cell Sci. 2011;124:2488-500 pubmed publisher
    ..Thus, our results point to an evolutionary conserved role for Ddc2/ATRIP in monitoring meiotic chromosome metabolism. ..
  60. Signon L, Malkova A, Naylor M, Klein H, Haber J. Genetic requirements for RAD51- and RAD54-independent break-induced replication repair of a chromosomal double-strand break. Mol Cell Biol. 2001;21:2048-56 pubmed
    ..We now report that gene conversion is also abolished when RAD54, RAD55, and RAD57 are deleted but BIR occurs, as with rad51Delta cells...
  61. Zhang X, Janke R, Kingsley J, Luo J, FASCHING C, Ehmsen K, et al. A conserved sequence extending motif III of the motor domain in the Snf2-family DNA translocase Rad54 is critical for ATPase activity. PLoS ONE. 2013;8:e82184 pubmed publisher
    b>Rad54 is a dsDNA-dependent ATPase that translocates on duplex DNA...
  62. Bilsland E, Hult M, Bell S, Sunnerhagen P, Downs J. The Bre5/Ubp3 ubiquitin protease complex from budding yeast contributes to the cellular response to DNA damage. DNA Repair (Amst). 2007;6:1471-84 pubmed
    ..Additionally, we have generated mutants of Bre5 that are specifically defective in DNA damage responses. ..
  63. Nimonkar A, Dombrowski C, Siino J, Stasiak A, Stasiak A, Kowalczykowski S. Saccharomyces cerevisiae Dmc1 and Rad51 proteins preferentially function with Tid1 and Rad54 proteins, respectively, to promote DNA strand invasion during genetic recombination. J Biol Chem. 2012;287:28727-37 pubmed publisher
    ..Dmc1, a meiosis-specific paralog of Rad51, mediates the pairing of homologous chromosomes. Tid1, a Rad54 paralog, although not meiosis-specific, interacts with Dmc1 and promotes crossover formation between homologs...
  64. Schär P, Fäsi M, Jessberger R. SMC1 coordinates DNA double-strand break repair pathways. Nucleic Acids Res. 2004;32:3921-9 pubmed
    ..Strikingly, inactivation of RAD52 or RAD54 fully rescued efficiency and accuracy of NHEJ in the smc1 background...
  65. Debrauwere H, Loeillet S, Lin W, Lopes J, Nicolas A. Links between replication and recombination in Saccharomyces cerevisiae: a hypersensitive requirement for homologous recombination in the absence of Rad27 activity. Proc Natl Acad Sci U S A. 2001;98:8263-9 pubmed
    ..This and previous studies indicate that homologous recombination is the primary, but not only, pathway that functions to bypass the replication defects that arise in the absence of the Rad27 protein. ..
  66. Chang M, Bellaoui M, Zhang C, Desai R, Morozov P, Delgado Cruzata L, et al. RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex. EMBO J. 2005;24:2024-33 pubmed
    ..In addition, rmi1Delta strains fail to fully activate Rad53 upon exposure to DNA-damaging agents, suggesting that Rmi1 is also an important part of the Rad53-dependent DNA damage response. ..
  67. Zanders S, Sonntag Brown M, Chen C, Alani E. Pch2 modulates chromatid partner choice during meiotic double-strand break repair in Saccharomyces cerevisiae. Genetics. 2011;188:511-21 pubmed publisher
    ..Results from genetic epistasis analyses involving spo13?, rad54?, and mek1/MEK1 alleles and an intersister recombination reporter assay are also consistent with Pch2 acting to ..
  68. Ivanov E, Sugawara N, Fishman Lobell J, Haber J. Genetic requirements for the single-strand annealing pathway of double-strand break repair in Saccharomyces cerevisiae. Genetics. 1996;142:693-704 pubmed
    ..We show that RAD51, RAD54, RAD55, and RAD57 genes are not required for SSA irrespective of whether recombination occurred in plasmid or ..
  69. Burgess R, Sebesta M, Sisakova A, Marini V, Lisby M, Damborsky J, et al. The PCNA interaction protein box sequence in Rad54 is an integral part of its ATPase domain and is required for efficient DNA repair and recombination. PLoS ONE. 2013;8:e82630 pubmed publisher
    b>Rad54 is an ATP-driven translocase involved in the genome maintenance pathway of homologous recombination (HR)...
  70. Gaines W, Godin S, Kabbinavar F, Rao T, VanDemark A, Sung P, et al. Promotion of presynaptic filament assembly by the ensemble of S. cerevisiae Rad51 paralogues with Rad52. Nat Commun. 2015;6:7834 pubmed publisher