RAD52

Summary

Gene Symbol: RAD52
Description: recombinase RAD52
Alias: recombinase RAD52
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Motegi A, Kuntz K, Majeed A, Smith S, Myung K. Regulation of gross chromosomal rearrangements by ubiquitin and SUMO ligases in Saccharomyces cerevisiae. Mol Cell Biol. 2006;26:1424-33 pubmed
    ..We propose a mechanism for how defects in these proteins could lead to diverse outcomes (proper repair or GCR formation) through different regulation of DNA repair machinery. ..
  2. Chang M, Bellaoui M, Zhang C, Desai R, Morozov P, Delgado Cruzata L, et al. RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex. EMBO J. 2005;24:2024-33 pubmed
    ..kinase, undergo a mitotic delay, and display increased relocalization of the recombination repair protein Rad52, indicating the presence of spontaneous DNA damage...
  3. Game J, Kaufman P. Role of Saccharomyces cerevisiae chromatin assembly factor-I in repair of ultraviolet radiation damage in vivo. Genetics. 1999;151:485-97 pubmed
    ..We find an increased loss of telomeric gene silencing in rad6Delta cac1Delta and rad18Delta cac1Delta double mutants, suggesting that CAF-I and multiple factors in the postreplicative repair pathway influence chromosome structure. ..
  4. Lee S, Moore J, Holmes A, Umezu K, Kolodner R, Haber J. Saccharomyces Ku70, mre11/rad50 and RPA proteins regulate adaptation to G2/M arrest after DNA damage. Cell. 1998;94:399-409 pubmed
    ..We suggest that escape from the DNA damage-induced G2/M checkpoint depends on the extent of ssDNA created at broken chromosome ends. RPA appears to play a key intermediate step in this adaptation. ..
  5. Lebel C, Larrivée M, Bah A, Laterreur N, Lvesque N, Wellinger R. Assessing telomeric phenotypes. Methods Mol Biol. 2006;313:265-316 pubmed
    ..However, growth phenotypes (senescence) and fine-structure analyses of the chromosome terminal DNA are also becoming increasingly important. ..
  6. Firmenich A, Elias Arnanz M, Berg P. A novel allele of Saccharomyces cerevisiae RFA1 that is deficient in recombination and repair and suppressible by RAD52. Mol Cell Biol. 1995;15:1620-31 pubmed
    ..Characterization of rfa1-44 revealed that it is, like members of the RAD52 epistasis group, sensitive to X rays, high doses of UV, and HO-induced DSBs...
  7. Lisby M, Barlow J, Burgess R, Rothstein R. Choreography of the DNA damage response: spatiotemporal relationships among checkpoint and repair proteins. Cell. 2004;118:699-713 pubmed
  8. Lao J, Oh S, Shinohara M, Shinohara A, Hunter N. Rad52 promotes postinvasion steps of meiotic double-strand-break repair. Mol Cell. 2008;29:517-24 pubmed publisher
    ..By contrast, the postinvasion steps of recombination are poorly characterized. Rad52 plays an essential role during early steps of recombination by mediating assembly of a RecA homolog, Rad51, into ..
  9. Pâques F, Haber J. Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae. Microbiol Mol Biol Rev. 1999;63:349-404 pubmed
    ..This review encompasses these different aspects of DSB-induced recombination in Saccharomyces and attempts to relate genetic, molecular biological, and biochemical studies of the processes of DNA repair and recombination. ..

More Information

Publications95

  1. O Neill B, Hanway D, Winzeler E, Romesberg F. Coordinated functions of WSS1, PSY2 and TOF1 in the DNA damage response. Nucleic Acids Res. 2004;32:6519-30 pubmed
    ..of WSS1 resulted in synergistic increases in UV sensitivity with null mutants of genes involved in recombination (RAD52) and cell cycle control (RAD9 and RAD24)...
  2. Noël J, Wellinger R. Abrupt telomere losses and reduced end-resection can explain accelerated senescence of Smc5/6 mutants lacking telomerase. DNA Repair (Amst). 2011;10:271-82 pubmed publisher
  3. Shinohara A, Shinohara M, Ohta T, Matsuda S, Ogawa T. Rad52 forms ring structures and co-operates with RPA in single-strand DNA annealing. Genes Cells. 1998;3:145-56 pubmed
    The RAD52 epistasis group in Saccharomyces cerevisiae is involved in various types of homologous recombination including recombinational double-strand break (DSB) repair and meiotic recombination...
  4. Chen C, Umezu K, Kolodner R. Chromosomal rearrangements occur in S. cerevisiae rfa1 mutator mutants due to mutagenic lesions processed by double-strand-break repair. Mol Cell. 1998;2:9-22 pubmed
    ..A representative rfa1 mutation exhibited a growth defect in conjunction with rad51, rad52, or rad10 mutations, suggesting an accumulation of double-strand breaks...
  5. Haracska L, Torres Ramos C, Johnson R, Prakash S, Prakash L. Opposing effects of ubiquitin conjugation and SUMO modification of PCNA on replicational bypass of DNA lesions in Saccharomyces cerevisiae. Mol Cell Biol. 2004;24:4267-74 pubmed
    ..In addition, we provide evidence for the activation of the RAD52 recombinational pathway in the pol30-119 mutant and we infer that SUMO conjugation at the lysine 164 residue of ..
  6. Kouprina N, Kroll E, Bannikov V, Bliskovsky V, Gizatullin R, Kirillov A, et al. CTF4 (CHL15) mutants exhibit defective DNA metabolism in the yeast Saccharomyces cerevisiae. Mol Cell Biol. 1992;12:5736-47 pubmed
    ..However, ctf4 mutants exhibit an accumulation of large budded cells with the nucleus in the neck. ctf4 rad52 double mutants grew very slowly and produced extremely high levels (50%) of inviable cell division products ..
  7. Lopes J, Ribeyre C, Nicolas A. Complex minisatellite rearrangements generated in the total or partial absence of Rad27/hFEN1 activity occur in a single generation and are Rad51 and Rad52 dependent. Mol Cell Biol. 2006;26:6675-89 pubmed
    ..complementation of the rad27Delta mutation by hFEN1 demonstrated that the production of novel CEB1 alleles is Rad52 and Rad51 dependent...
  8. Yang J, Freudenreich C. The Rtt109 histone acetyltransferase facilitates error-free replication to prevent CAG/CTG repeat contractions. DNA Repair (Amst). 2010;9:414-20 pubmed publisher
  9. Wu Y, Sugiyama T, Kowalczykowski S. DNA annealing mediated by Rad52 and Rad59 proteins. J Biol Chem. 2006;281:15441-9 pubmed
    In the budding yeast Saccharomyces cerevisiae, the RAD52 gene is essential for all homologous recombination events and its homologue, the RAD59 gene, is important for those that occur independently of RAD51...
  10. Ball L, Zhang K, Cobb J, Boone C, Xiao W. The yeast Shu complex couples error-free post-replication repair to homologous recombination. Mol Microbiol. 2009;73:89-102 pubmed publisher
    ..This mechanism appears to be conserved throughout eukaryotes. ..
  11. Bai Y, Symington L. A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae. Genes Dev. 1996;10:2025-37 pubmed
    ..as a recombination reporter we have previously shown that mitotic recombination is dependent on the RAD52 gene...
  12. McDonald J, Rothstein R. Unrepaired heteroduplex DNA in Saccharomyces cerevisiae is decreased in RAD1 RAD52-independent recombination. Genetics. 1994;137:393-405 pubmed
    ..Unrepaired heteroduplex increases significantly only in rad52 mutant strains...
  13. Khadaroo B, Teixeira M, Luciano P, Eckert Boulet N, Germann S, Simon M, et al. The DNA damage response at eroded telomeres and tethering to the nuclear pore complex. Nat Cell Biol. 2009;11:980-7 pubmed publisher
    ..Cdc13 (cell division cycle 13), replication protein A, DNA damage checkpoint proteins and the DNA repair protein Rad52 into a single focus...
  14. Hwang J, Smith S, Ceschia A, Torres Rosell J, Aragon L, Myung K. Smc5-Smc6 complex suppresses gross chromosomal rearrangements mediated by break-induced replications. DNA Repair (Amst). 2008;7:1426-36 pubmed publisher
  15. Tishkoff D, Filosi N, Gaida G, Kolodner R. A novel mutation avoidance mechanism dependent on S. cerevisiae RAD27 is distinct from DNA mismatch repair. Cell. 1997;88:253-63 pubmed
    ..in RAD27 cause increased rates of mitotic crossing over and are lethal in combination with mutations in RAD51 and RAD52. These observations suggest that the majority of replication errors that accumulate in rad27 strains are processed ..
  16. Shi I, Hallwyl S, Seong C, Mortensen U, Rothstein R, Sung P. Role of the Rad52 amino-terminal DNA binding activity in DNA strand capture in homologous recombination. J Biol Chem. 2009;284:33275-84 pubmed publisher
    Saccharomyces cerevisiae Rad52 protein promotes homologous recombination by nucleating the Rad51 recombinase onto replication protein A-coated single-stranded DNA strands and also by directly annealing such strands...
  17. Kanellis P, Gagliardi M, Banath J, Szilard R, Nakada S, Galicia S, et al. A screen for suppressors of gross chromosomal rearrangements identifies a conserved role for PLP in preventing DNA lesions. PLoS Genet. 2007;3:e134 pubmed
  18. Hwang J, Smith S, Myung K. The Rad1-Rad10 complex promotes the production of gross chromosomal rearrangements from spontaneous DNA damage in Saccharomyces cerevisiae. Genetics. 2005;169:1927-37 pubmed
    ..Results presented here suggest that Rad1-Rad10 functions at different stages of GCR formation and that there is an alternative pathway for the GCR formation that is independent of Rad1-Rad10. ..
  19. Linger J, Tyler J. The yeast histone chaperone chromatin assembly factor 1 protects against double-strand DNA-damaging agents. Genetics. 2005;171:1513-22 pubmed
    ..We propose that CAF-1 has an essential role in assembling chromatin during double-strand-DNA repair. ..
  20. Chai B, Huang J, Cairns B, Laurent B. Distinct roles for the RSC and Swi/Snf ATP-dependent chromatin remodelers in DNA double-strand break repair. Genes Dev. 2005;19:1656-61 pubmed
    ..We show that Swi/Snf is required earlier, at or preceding the strand invasion step of HR, while RSC is required following synapsis for completion of the recombinational repair event. ..
  21. Lettier G, Feng Q, de Mayolo A, Erdeniz N, Reid R, Lisby M, et al. The role of DNA double-strand breaks in spontaneous homologous recombination in S. cerevisiae. PLoS Genet. 2006;2:e194 pubmed
    ..Specifically, we describe a class of rad52 mutants that are fully proficient in inter- and intra-chromosomal mitotic HR, yet at the same time fail to repair ..
  22. Xiong L, Chen X, Silver H, Ahmed N, Johnson E. Deficient SUMO attachment to Flp recombinase leads to homologous recombination-dependent hyperamplification of the yeast 2 microm circle plasmid. Mol Biol Cell. 2009;20:1241-51 pubmed publisher
    ..This work also illustrates the importance of using cir(o) strains when studying mutants that affect the yeast SUMO pathway, to avoid confusing direct functions of the SUMO pathway with secondary effects of 2 microm amplification. ..
  23. Putnam C, Hayes T, Kolodner R. Post-replication repair suppresses duplication-mediated genome instability. PLoS Genet. 2010;6:e1000933 pubmed publisher
    ..Our analysis is consistent with models in which PRR prevents replication damage from becoming double strand breaks (DSBs) and/or regulates the activity of HR on DSBs. ..
  24. Hays S, Firmenich A, Massey P, Banerjee R, Berg P. Studies of the interaction between Rad52 protein and the yeast single-stranded DNA binding protein RPA. Mol Cell Biol. 1998;18:4400-6 pubmed
    ..a number of impaired recombination and repair phenotypes, all of which are suppressible by overexpression of RAD52. We demonstrate that a rad52 mutation is epistatic to the rfa1-44 mutation, placing RFA1 and RAD52 in the same ..
  25. Ye J, Ai X, Eugeni E, Zhang L, Carpenter L, Jelinek M, et al. Histone H4 lysine 91 acetylation a core domain modification associated with chromatin assembly. Mol Cell. 2005;18:123-30 pubmed
    ..These results indicate an important role for histone modifications outside the NH2-tail domains in the processes of chromatin assembly, DNA repair, and transcriptional silencing. ..
  26. González Prieto R, Muñoz Cabello A, Cabello Lobato M, Prado F. Rad51 replication fork recruitment is required for DNA damage tolerance. EMBO J. 2013;32:1307-21 pubmed publisher
    ..Here, we have studied the role of the recombination proteins Rad51 and Rad52 at replication forks and replicative DNA lesions...
  27. Ohuchi T, Seki M, Branzei D, Maeda D, Ui A, Ogiwara H, et al. Rad52 sumoylation and its involvement in the efficient induction of homologous recombination. DNA Repair (Amst). 2008;7:879-89 pubmed publisher
    The protein Rad52 is a key player in various types of homologous recombination and is essential to maintenance of genomic integrity...
  28. Lazzaro F, Sapountzi V, Granata M, Pellicioli A, Vaze M, Haber J, et al. Histone methyltransferase Dot1 and Rad9 inhibit single-stranded DNA accumulation at DSBs and uncapped telomeres. EMBO J. 2008;27:1502-12 pubmed publisher
  29. Smith J, Rothstein R. A mutation in the gene encoding the Saccharomyces cerevisiae single-stranded DNA-binding protein Rfa1 stimulates a RAD52-independent pathway for direct-repeat recombination. Mol Cell Biol. 1995;15:1632-41 pubmed
    In the yeast Saccharomyces cerevisiae, recombination between direct repeats is synergistically reduced in rad1 rad52 double mutants, suggesting that the two genes define alternate recombination pathways...
  30. Lendvay T, Morris D, Sah J, Balasubramanian B, Lundblad V. Senescence mutants of Saccharomyces cerevisiae with a defect in telomere replication identify three additional EST genes. Genetics. 1996;144:1399-412 pubmed
  31. Feng Q, Düring L, de Mayolo A, Lettier G, Lisby M, Erdeniz N, et al. Rad52 and Rad59 exhibit both overlapping and distinct functions. DNA Repair (Amst). 2007;6:27-37 pubmed
    ..In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous ..
  32. Colavito S, Macris Kiss M, Seong C, Gleeson O, Greene E, Klein H, et al. Functional significance of the Rad51-Srs2 complex in Rad51 presynaptic filament disruption. Nucleic Acids Res. 2009;37:6754-64 pubmed publisher
    ..Several of these phenotypes can be suppressed by inactivating genes of the RAD52 epistasis group that promote homologous recombination, implicating inappropriate recombination as the underlying ..
  33. Lewis L, Westmoreland J, Resnick M. Repair of endonuclease-induced double-strand breaks in Saccharomyces cerevisiae: essential role for genes associated with nonhomologous end-joining. Genetics. 1999;152:1513-29 pubmed
    ..In contrast, EcoRI caused prolonged cell-cycle arrest of recombination-defective rad51, rad52, rad54, rad55, and rad57 mutants, but cells remained viable...
  34. Saparbaev M, Prakash L, Prakash S. Requirement of mismatch repair genes MSH2 and MSH3 in the RAD1-RAD10 pathway of mitotic recombination in Saccharomyces cerevisiae. Genetics. 1996;142:727-36 pubmed
    ..Coupling of mismatch binding proteins with the recombinational machinery could be important for ensuring genetic fidelity in the recombination process. ..
  35. Torres J, Schnakenberg S, Zakian V. Saccharomyces cerevisiae Rrm3p DNA helicase promotes genome integrity by preventing replication fork stalling: viability of rrm3 cells requires the intra-S-phase checkpoint and fork restart activities. Mol Cell Biol. 2004;24:3198-212 pubmed
    ..The rrm3 system provides a unique opportunity to learn the fate of forks whose progress is impaired by natural impediments rather than by exogenous DNA damage. ..
  36. Smith J, Rothstein R. An allele of RFA1 suppresses RAD52-dependent double-strand break repair in Saccharomyces cerevisiae. Genetics. 1999;151:447-58 pubmed
    ..DNA-binding complex RP-A, was identified as a suppressor of decreased direct-repeat recombination in rad1 rad52 double mutants...
  37. Clemente Ruiz M, Prado F. Chromatin assembly controls replication fork stability. EMBO Rep. 2009;10:790-6 pubmed publisher
    ..This collapse is also associated with an accumulation of Rad52-dependent X-shaped molecules...
  38. Lin Y, Chang C, Wong C, Teng S. Recruitment of Rad51 and Rad52 to short telomeres triggers a Mec1-mediated hypersensitivity to double-stranded DNA breaks in senescent budding yeast. PLoS ONE. 2009;4:e8224 pubmed publisher
    ..that when cells equipped with short telomeres, recruitments of homologous recombination proteins, Rad51 and Rad52, were reduced at an HO-endonuclease-catalyzed double-strand break (DSB), while their associations were increased ..
  39. Chen Q, Ijpma A, Greider C. Two survivor pathways that allow growth in the absence of telomerase are generated by distinct telomere recombination events. Mol Cell Biol. 2001;21:1819-27 pubmed
    ..Two types of survivors, type I and type II, can be distinguished by their characteristic telomere patterns. RAD52 is essential for the generation of both types of survivors...
  40. McVey M, Kaeberlein M, Tissenbaum H, Guarente L. The short life span of Saccharomyces cerevisiae sgs1 and srs2 mutants is a composite of normal aging processes and mitotic arrest due to defective recombination. Genetics. 2001;157:1531-42 pubmed
    ..This arrest can be suppressed by mutations in RAD51, RAD52, and RAD57, suggesting that the cell cycle defect in sgs1 srs2 mutants results from inappropriate homologous ..
  41. Moriel Carretero M, Aguilera A. A postincision-deficient TFIIH causes replication fork breakage and uncovers alternative Rad51- or Pol32-mediated restart mechanisms. Mol Cell. 2010;37:690-701 pubmed publisher
    ..Broken forks are rescued by MRX-Rad52-Rfc1-dependent recombination via two types of replication restart mechanisms, one being Rad51 dependent and the ..
  42. Sung P. Function of yeast Rad52 protein as a mediator between replication protein A and the Rad51 recombinase. J Biol Chem. 1997;272:28194-7 pubmed
    The RAD51 and RAD52 genes of Saccharomyces cerevisiae are key members of the RAD52 epistasis group required for genetic recombination and the repair of DNA double-stranded breaks...
  43. Vance J, Wilson T. Yeast Tdp1 and Rad1-Rad10 function as redundant pathways for repairing Top1 replicative damage. Proc Natl Acad Sci U S A. 2002;99:13669-74 pubmed
    ..analysis revealed that both Tdp1 and Rad1-Rad10 repair proceed through recombination that equally depends on RAD52, RAD51, and RAD50...
  44. Shim E, Ma J, Oum J, Yanez Y, Lee S. The yeast chromatin remodeler RSC complex facilitates end joining repair of DNA double-strand breaks. Mol Cell Biol. 2005;25:3934-44 pubmed
    ..The interaction of Rsc1p with Mre11p appears to be vital for survival from genotoxic stress. These results suggest that chromatin remodeling by RSC is important for NHEJ. ..
  45. Bai Y, Davis A, Symington L. A novel allele of RAD52 that causes severe DNA repair and recombination deficiencies only in the absence of RAD51 or RAD59. Genetics. 1999;153:1117-30 pubmed
    ..inverted repeat as a recombination reporter, we have shown that mitotic recombination is dependent on the RAD52 gene, but reduced only fivefold by mutation of RAD51...
  46. Krejci L, Damborsky J, Thomsen B, Duno M, Bendixen C. Molecular dissection of interactions between Rad51 and members of the recombination-repair group. Mol Cell Biol. 2001;21:966-76 pubmed
    ..In the yeast Saccharomyces cerevisiae, recombination requires products of the RAD52 epistasis group...
  47. Krejci L, Van Komen S, Li Y, Villemain J, Reddy M, Klein H, et al. DNA helicase Srs2 disrupts the Rad51 presynaptic filament. Nature. 2003;423:305-9 pubmed
  48. Mbantenkhu M, Wang X, Nardozzi J, Wilkens S, Hoffman E, Patel A, et al. Mgm101 is a Rad52-related protein required for mitochondrial DNA recombination. J Biol Chem. 2011;286:42360-70 pubmed publisher
    ..Here, we show that the yeast mitochondrial nucleoid protein, Mgm101, is related to the Rad52-type recombination proteins that are widespread in organisms from bacteriophage to humans...
  49. Gangavarapu V, Prakash S, Prakash L. Requirement of RAD52 group genes for postreplication repair of UV-damaged DNA in Saccharomyces cerevisiae. Mol Cell Biol. 2007;27:7758-64 pubmed
    ..Here, we examine the contributions of the RAD51, RAD52, and RAD54 genes and of the RAD50 and XRS2 genes to the PRR of UV-damaged DNA...
  50. Lebel C, Rosonina E, Sealey D, Pryde F, Lydall D, Maringele L, et al. Telomere maintenance and survival in saccharomyces cerevisiae in the absence of telomerase and RAD52. Genetics. 2009;182:671-84 pubmed publisher
    ..strain W303, telomerase-null populations bypass senescence and, unless EXO1 is also deleted, this survival is RAD52 dependent. Unexpectedly, we found that the S...
  51. Thomas B, Rothstein R. The genetic control of direct-repeat recombination in Saccharomyces: the effect of rad52 and rad1 on mitotic recombination at GAL10, a transcriptionally regulated gene. Genetics. 1989;123:725-38 pubmed
    ..We have examined the role of two recombination- and repair-defective mutations, rad1 and rad52, on direct repeat recombination in transcriptionally active and inactive sequences...
  52. Halas A, Podlaska A, Derkacz J, McIntyre J, Skoneczna A, Sledziewska Gojska E. The roles of PCNA SUMOylation, Mms2-Ubc13 and Rad5 in translesion DNA synthesis in Saccharomyces cerevisiae. Mol Microbiol. 2011;80:786-97 pubmed publisher
    ..carrying rad5 and/or mms2 mutations is connected with the known role of PCNA SUMOylation in the inhibition of Rad52-mediated recombination...
  53. Ben Aroya S, Koren A, Liefshitz B, Steinlauf R, Kupiec M. ELG1, a yeast gene required for genome stability, forms a complex related to replication factor C. Proc Natl Acad Sci U S A. 2003;100:9906-11 pubmed
    ..Genetic data indicate that the Elg1, Ctf18, and Rad24 RFC-like complexes work in three separate pathways important for maintaining the integrity of the genome and for coping with various genomic stresses. ..
  54. Toczyski D, Galgoczy D, Hartwell L. CDC5 and CKII control adaptation to the yeast DNA damage checkpoint. Cell. 1997;90:1097-106 pubmed
  55. Ramey C, Howar S, Adkins M, Linger J, Spicer J, Tyler J. Activation of the DNA damage checkpoint in yeast lacking the histone chaperone anti-silencing function 1. Mol Cell Biol. 2004;24:10313-27 pubmed
  56. Torres Rosell J, Sunjevaric I, De Piccoli G, Sacher M, Eckert Boulet N, Reid R, et al. The Smc5-Smc6 complex and SUMO modification of Rad52 regulates recombinational repair at the ribosomal gene locus. Nat Cell Biol. 2007;9:923-31 pubmed
    ..The nucleolar exclusion of Rad52 recombination foci entails Mre11 and Smc5-Smc6 complexes and depends on Rad52 SUMO (small ubiquitin-related ..
  57. Lisby M, Rothstein R, Mortensen U. Rad52 forms DNA repair and recombination centers during S phase. Proc Natl Acad Sci U S A. 2001;98:8276-82 pubmed
    ..The key role played by Rad52 in this pathway has been attributed to its ability to seek out and mediate annealing of homologous DNA strands...
  58. Ma J, Kim E, Haber J, Lee S. Yeast Mre11 and Rad1 proteins define a Ku-independent mechanism to repair double-strand breaks lacking overlapping end sequences. Mol Cell Biol. 2003;23:8820-8 pubmed
    ..The MMEJ also occurs when Rad52 is absent, but the extent of deletions becomes more limited...
  59. Cortes Ledesma F, Malagon F, Aguilera A. A novel yeast mutation, rad52-L89F, causes a specific defect in Rad51-independent recombination that correlates with a reduced ability of Rad52-L89F to interact with Rad59. Genetics. 2004;168:553-7 pubmed
    We isolated a novel rad52 mutation, rad52-L89F, which specifically impairs recombination in rad51Delta cells...
  60. Prakash L. Characterization of postreplication repair in Saccharomyces cerevisiae and effects of rad6, rad18, rev3 and rad52 mutations. Mol Gen Genet. 1981;184:471-8 pubmed
    ..The rad6 mutant does not carry out postreplication repair, the rad18 and rad52 mutants show great inhibition while the rev3 mutation does not affect postreplication repair...
  61. Pfander B, Moldovan G, Sacher M, Hoege C, Jentsch S. SUMO-modified PCNA recruits Srs2 to prevent recombination during S phase. Nature. 2005;436:428-33 pubmed
    ..Our finding suggests a model in which SUMO-modified PCNA recruits Srs2 in S phase in order to prevent unwanted recombination events of replicating chromosomes. ..
  62. Tsukuda T, Lo Y, Krishna S, Sterk R, Osley M, Nickoloff J. INO80-dependent chromatin remodeling regulates early and late stages of mitotic homologous recombination. DNA Repair (Amst). 2009;8:360-9 pubmed publisher
    ..DSB repair by HR shows a modest defect that is partially suppressed by overexpression of Rad51 or its mediator, Rad52. In wild type cells, DSB repair typically results in gene conversion, and most gene conversion tracts are ..
  63. Burgess R, Rahman S, Lisby M, Rothstein R, Zhao X. The Slx5-Slx8 complex affects sumoylation of DNA repair proteins and negatively regulates recombination. Mol Cell Biol. 2007;27:6153-62 pubmed
    ..In addition, the complex inhibits Rad51-independent recombination via modulating the sumoylation of DNA repair proteins. ..
  64. Chen C, Motegi A, Hasegawa Y, Myung K, Kolodner R, D ANDREA A. Genetic analysis of ionizing radiation-induced mutagenesis in Saccharomyces cerevisiae reveals TransLesion Synthesis (TLS) independent of PCNA K164 SUMOylation and ubiquitination. DNA Repair (Amst). 2006;5:1475-88 pubmed
    ..A genetic model based on these observations is proposed. ..
  65. Soustelle C, Vernis L, Fréon K, Reynaud Angelin A, Chanet R, Fabre F, et al. A new Saccharomyces cerevisiae strain with a mutant Smt3-deconjugating Ulp1 protein is affected in DNA replication and requires Srs2 and homologous recombination for its viability. Mol Cell Biol. 2004;24:5130-43 pubmed
    ..These structures are believed to generate different recombination intermediates. Some of them are fixed by recombination, and others require Srs2 to be reversed and fixed by an alternate pathway. ..
  66. Putnam C, Hayes T, Kolodner R. Specific pathways prevent duplication-mediated genome rearrangements. Nature. 2009;460:984-9 pubmed publisher
    ..This explains how extensive genome instability is prevented in eukaryotic cells whose genomes contain numerous divergent repeated sequences...
  67. Plate I, Hallwyl S, Shi I, Krejci L, Muller C, Albertsen L, et al. Interaction with RPA is necessary for Rad52 repair center formation and for its mediator activity. J Biol Chem. 2008;283:29077-85 pubmed publisher
    ..HR is catalyzed by proteins encoded by genes of the RAD52 epistasis group, including the recombinase Rad51 and its mediator Rad52...
  68. Meyer D, Bailis A. Telomerase deficiency affects the formation of chromosomal translocations by homologous recombination in Saccharomyces cerevisiae. PLoS ONE. 2008;3:e3318 pubmed publisher
    ..This decrease correlated with a sequestration of the central homologous recombination factor, Rad52, to telomeres determined by chromatin immuno-precipitation...
  69. Wurtele H, Kaiser G, Bacal J, St Hilaire E, Lee E, Tsao S, et al. Histone H3 lysine 56 acetylation and the response to DNA replication fork damage. Mol Cell Biol. 2012;32:154-72 pubmed publisher
    ..DNA replication and eventually segregate chromosomes with intranuclear foci containing the recombination protein Rad52. In addition, we provide evidence that these phenotypes are not due to defects in base excision repair, defects in ..
  70. Bergink S, Ammon T, Kern M, Schermelleh L, Leonhardt H, Jentsch S. Role of Cdc48/p97 as a SUMO-targeted segregase curbing Rad51-Rad52 interaction. Nat Cell Biol. 2013;15:526-32 pubmed publisher
    ..Cdc48 associates with SUMOylated Rad52, a factor that assembles the Rad51 recombinase on chromatin...
  71. Abdallah P, Luciano P, Runge K, Lisby M, Geli V, Gilson E, et al. A two-step model for senescence triggered by a single critically short telomere. Nat Cell Biol. 2009;11:988-93 pubmed publisher
    ..This pre-senescence growth requires RAD52 (radiation sensitive) and MMS1 (methyl methane sulfonate sensitive), and there is no evidence for major inter-..
  72. Seong C, Sehorn M, Plate I, Shi I, Song B, Chi P, et al. Molecular anatomy of the recombination mediator function of Saccharomyces cerevisiae Rad52. J Biol Chem. 2008;283:12166-74 pubmed publisher
    ..b>Rad52 facilitates presynaptic filament assembly, and this recombination mediator activity is thought to rely on the ..
  73. Miyazaki T, Bressan D, Shinohara M, Haber J, Shinohara A. In vivo assembly and disassembly of Rad51 and Rad52 complexes during double-strand break repair. EMBO J. 2004;23:939-49 pubmed
    Assembly and disassembly of Rad51 and Rad52 complexes were monitored by immunofluorescence during homologous recombination initiated by an HO endonuclease-induced double-strand break (DSB) at the MAT locus...
  74. Ribeyre C, Lopes J, Boulé J, Piazza A, Guédin A, Zakian V, et al. The yeast Pif1 helicase prevents genomic instability caused by G-quadruplex-forming CEB1 sequences in vivo. PLoS Genet. 2009;5:e1000475 pubmed publisher
    ..Hence, we conclude that CEB1 instability in pif1Delta cells depends on the potential to form G-quadruplex structures, suggesting that Pif1 could play a role in the metabolism of G4-forming sequences. ..
  75. Zhang W, Durocher D. De novo telomere formation is suppressed by the Mec1-dependent inhibition of Cdc13 accumulation at DNA breaks. Genes Dev. 2010;24:502-15 pubmed publisher
    ..These studies therefore identify a mechanism by which the ATR family of kinases enforces genome integrity, and a process that underscores the contribution of Cdc13 to the fate of DNA ends. ..
  76. Li F, Dong J, Pan X, Oum J, Boeke J, Lee S. Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates. Mol Cell. 2008;30:325-35 pubmed publisher
    ..Saw1 interacts physically with Rad1/Rad10, Msh2/Msh3, and Rad52 proteins, and cells lacking SLX4 or SAW1 accumulate recombination intermediates blocked at the Rad1/Rad10-..
  77. Pike B, Heierhorst J. Mdt1 facilitates efficient repair of blocked DNA double-strand breaks and recombinational maintenance of telomeres. Mol Cell Biol. 2007;27:6532-45 pubmed
  78. Wu Y, Siino J, Sugiyama T, Kowalczykowski S. The DNA binding preference of RAD52 and RAD59 proteins: implications for RAD52 and RAD59 protein function in homologous recombination. J Biol Chem. 2006;281:40001-9 pubmed
    We examined the double-stranded DNA (dsDNA) binding preference of the Saccharomyces cerevisiae Rad52 protein and its homologue, the Rad59 protein...
  79. Arai N, Kagawa W, Saito K, Shingu Y, Mikawa T, Kurumizaka H, et al. Vital roles of the second DNA-binding site of Rad52 protein in yeast homologous recombination. J Biol Chem. 2011;286:17607-17 pubmed publisher
    ..b>Rad52 is the prototype of recombination mediators, and the human Rad52 protein has two distinct DNA-binding sites: the ..
  80. Hang L, Liu X, Cheung I, Yang Y, Zhao X. SUMOylation regulates telomere length homeostasis by targeting Cdc13. Nat Struct Mol Biol. 2011;18:920-6 pubmed publisher
  81. Guzder S, Sommers C, Prakash L, Prakash S. Complex formation with damage recognition protein Rad14 is essential for Saccharomyces cerevisiae Rad1-Rad10 nuclease to perform its function in nucleotide excision repair in vivo. Mol Cell Biol. 2006;26:1135-41 pubmed
    ..We discuss the implications of these observations for the means by which the different NER proteins are assembled at the lesion site. ..
  82. Radford S, Boyle M, Sheely C, Graham J, Haeusser D, Zimmerman L, et al. Increase in Ty1 cDNA recombination in yeast sir4 mutant strains at high temperature. Genetics. 2004;168:89-101 pubmed
    ..the apparent increase in transposition activity in sir4 mutant strains at high temperature is dependent on the RAD52 gene and is thus likely resulting from an increase in Ty1 cDNA recombination, rather than in IN-mediated ..
  83. Hegde V, Klein H. Requirement for the SRS2 DNA helicase gene in non-homologous end joining in yeast. Nucleic Acids Res. 2000;28:2779-83 pubmed
    ..However, NHEJ of blunt ends, while very inefficient, is not further reduced by mutations in YKU70, SIR2, SIR3, SIR4 or SRS2, suggesting that this rejoining process occurs by a different mechanism. ..
  84. de Mayolo A, Sunjevaric I, Reid R, Mortensen U, Rothstein R, Lisby M. The rad52-Y66A allele alters the choice of donor template during spontaneous chromosomal recombination. DNA Repair (Amst). 2010;9:23-32 pubmed publisher
    ..In this study we have used a rad52 hyper-recombination mutant, rad52-Y66A, to investigate the process of spontaneous heteroallelic recombination in ..
  85. Kerrest A, Anand R, Sundararajan R, Bermejo R, Liberi G, Dujon B, et al. SRS2 and SGS1 prevent chromosomal breaks and stabilize triplet repeats by restraining recombination. Nat Struct Mol Biol. 2009;16:159-67 pubmed publisher
    ..Deletion of RAD52 or RAD51 suppresses these phenotypes, suggesting that recombination triggers trinucleotide repeat instability in ..
  86. Altmannova V, Eckert Boulet N, Arneric M, Kolesar P, Chaloupkova R, Damborsky J, et al. Rad52 SUMOylation affects the efficiency of the DNA repair. Nucleic Acids Res. 2010;38:4708-21 pubmed publisher
    ..effect of SUMOylation on the biochemical properties of the Saccharomyces cerevisiae recombination mediator protein Rad52. Interestingly, Rad52 SUMOylation is enhanced by single-stranded DNA, and we show that SUMOylation of Rad52 also ..