Genomes and Genes
Gene Symbol: RAD30
Description: DNA-directed DNA polymerase eta
Alias: DBH1, DNA-directed DNA polymerase eta
Species: Saccharomyces cerevisiae S288c
- Broomfield S, Xiao W. Suppression of genetic defects within the RAD6 pathway by srs2 is specific for error-free post-replication repair but not for damage-induced mutagenesis. Nucleic Acids Res. 2002;30:732-9 pubmed..Taken together, our observations link error-free PRR to homologous recombination through the helicase activity of Srs2. ..
- Johnson R, Prakash S, Prakash L. Efficient bypass of a thymine-thymine dimer by yeast DNA polymerase, Poleta. Science. 1999;283:1001-4 pubmedThe RAD30 gene of the yeast Saccharomyces cerevisiae is required for the error-free postreplicational repair of DNA that has been damaged by ultraviolet irradiation...
- Parker J, Bielen A, Dikic I, Ulrich H. Contributions of ubiquitin- and PCNA-binding domains to the activity of Polymerase eta in Saccharomyces cerevisiae. Nucleic Acids Res. 2007;35:881-9 pubmed..of ubiquitin and PCNA binding to damage bypass and damage-induced mutagenesis in Polymerase eta (encoded by RAD30) from the budding yeast Saccharomyces cerevisiae...
- Haracska L, Yu S, Johnson R, Prakash L, Prakash S. Efficient and accurate replication in the presence of 7,8-dihydro-8-oxoguanine by DNA polymerase eta. Nat Genet. 2000;25:458-61 pubmed..The gene RAD30 of S...
- McDonald J, Levine A, Woodgate R. The Saccharomyces cerevisiae RAD30 gene, a homologue of Escherichia coli dinB and umuC, is DNA damage inducible and functions in a novel error-free postreplication repair mechanism. Genetics. 1997;147:1557-68 pubmed..are structurally and/or functionally conserved between prokaryotes and eukaryotes, we investigated the role of RAD30, a previously uncharacterized Saccharomyces cerevisiae DNA repair gene related to the Escherichia coli dinB, umuC ..
- Zhao B, Xie Z, Shen H, Wang Z. Role of DNA polymerase eta in the bypass of abasic sites in yeast cells. Nucleic Acids Res. 2004;32:3984-94 pubmed..In cells lacking Poleta (rad30), Rev1, Polzeta (rev3), and both Poleta and Polzeta, translesion synthesis was reduced to 30%, 30%, 15% and 3% of ..
- Stelter P, Ulrich H. Control of spontaneous and damage-induced mutagenesis by SUMO and ubiquitin conjugation. Nature. 2003;425:188-91 pubmed..Our findings assign a function to SUMO during S phase and demonstrate how ubiquitin and SUMO, by regulating the accuracy of replication and repair, contribute to overall genomic stability. ..
- Sharma N, Kochenova O, Shcherbakova P. The non-canonical protein binding site at the monomer-monomer interface of yeast proliferating cell nuclear antigen (PCNA) regulates the Rev1-PCNA interaction and Pol?/Rev1-dependent translesion DNA synthesis. J Biol Chem. 2011;286:33557-66 pubmed publisher..The new mode of Rev1-PCNA binding described here suggests a mechanism by which Rev1 adopts a catalytically inactive configuration at the replication fork. ..
- Daigaku Y, Davies A, Ulrich H. Ubiquitin-dependent DNA damage bypass is separable from genome replication. Nature. 2010;465:951-5 pubmed publisher..Our approach has revealed the distribution of PRR tracts in a synchronized cell population. It will allow an in-depth mechanistic analysis of how cells manage the processing of lesions to their genomes during and after replication. ..
- Pages V, Bresson A, Acharya N, Prakash S, Fuchs R, Prakash L. Requirement of Rad5 for DNA polymerase zeta-dependent translesion synthesis in Saccharomyces cerevisiae. Genetics. 2008;180:73-82 pubmed publisher..Rad5 action in this role is likely to be structural, since neither the inactivation of its ubiquitin ligase activity nor the inactivation of its helicase activity impairs its role in TLS. ..
- Gibbs P, McDonald J, Woodgate R, Lawrence C. The relative roles in vivo of Saccharomyces cerevisiae Pol eta, Pol zeta, Rev1 protein and Pol32 in the bypass and mutation induction of an abasic site, T-T (6-4) photoadduct and T-T cis-syn cyclobutane dimer. Genetics. 2005;169:575-82 pubmed..of an abasic site, T-T (6-4) photoadduct and T-T cis-syn cyclobutane dimer, by transforming strains deleted for RAD30, REV3, REV1, or POL32 with duplex plasmids carrying one of these DNA lesions located within a 28-nucleotide single-..
- Haracska L, Prakash S, Prakash L. Replication past O(6)-methylguanine by yeast and human DNA polymerase eta. Mol Cell Biol. 2000;20:8001-7 pubmed..of MNNG-induced mutations is almost abolished in the rad30Delta pol32Delta double mutant of yeast, which lacks the RAD30 gene that encodes Poleta and the Pol32 subunit of DNA polymerase delta (Poldelta)...
- Acharya N, Haracska L, Prakash S, Prakash L. Complex formation of yeast Rev1 with DNA polymerase eta. Mol Cell Biol. 2007;27:8401-8 pubmed..We also found that the DNA synthesis activity of Rev1 is enhanced in this complex. We discuss the implications of these and other observations for the possible divergence of Rev1's structural role between yeast and humans. ..
- Abdulovic A, Jinks Robertson S. The in vivo characterization of translesion synthesis across UV-induced lesions in Saccharomyces cerevisiae: insights into Pol zeta- and Pol eta-dependent frameshift mutagenesis. Genetics. 2006;172:1487-98 pubmed..Finally, the examination of frameshift reversion spectra indicates a hierarchy between Pol eta and Pol zeta with respect to the bypass of UV-induced lesions. ..
- Xiao W, Chow B, Broomfield S, Hanna M. The Saccharomyces cerevisiae RAD6 group is composed of an error-prone and two error-free postreplication repair pathways. Genetics. 2000;155:1633-41 pubmed..In view of the high degree of sequence conservation of the RAD6 pathway genes among all eukaryotes, the model presented in this study may also apply to mammalian cells and predicts links to human diseases. ..
- Pavlov Y, Nguyen D, Kunkel T. Mutator effects of overproducing DNA polymerase eta (Rad30) and its catalytically inactive variant in yeast. Mutat Res. 2001;478:129-39 pubmed..Based on this, here we report the effects of deletion or increased expression of yeast RAD30 gene, encoding for polymerase eta (Pol eta), on spontaneous mutagenesis in vivo...
- Gangavarapu V, Prakash S, Prakash L. Requirement of RAD52 group genes for postreplication repair of UV-damaged DNA in Saccharomyces cerevisiae. Mol Cell Biol. 2007;27:7758-64 pubmed..In addition, our results suggest a role for the Rad50 and Xrs2 proteins and thereby for the MRX complex in promoting PRR via both the Rad5 and Rad52 pathways. ..
- Ulrich H, Jentsch S. Two RING finger proteins mediate cooperation between ubiquitin-conjugating enzymes in DNA repair. EMBO J. 2000;19:3388-97 pubmed..Surprisingly, UBC13 and MMS2 are largely cytosolic proteins, but DNA damage triggers their redistribution to the nucleus. These findings suggest a mechanism by which the activity of this DNA repair pathway could be regulated. ..
- Zhuang Z, Johnson R, Haracska L, Prakash L, Prakash S, Benkovic S. Regulation of polymerase exchange between Poleta and Poldelta by monoubiquitination of PCNA and the movement of DNA polymerase holoenzyme. Proc Natl Acad Sci U S A. 2008;105:5361-6 pubmed publisher..Thus the removal of the ubiquitin moiety from PCNA is likely required for the reverse exchange step after the lesion bypass synthesis by Poleta. ..
- Karras G, Jentsch S. The RAD6 DNA damage tolerance pathway operates uncoupled from the replication fork and is functional beyond S phase. Cell. 2010;141:255-67 pubmed publisher..We therefore propose that the RAD6 pathway acts on single-stranded gaps left behind newly restarted replication forks. ..
- Johnson R, Yu S, Prakash S, Prakash L. A role for yeast and human translesion synthesis DNA polymerases in promoting replication through 3-methyl adenine. Mol Cell Biol. 2007;27:7198-205 pubmed..We discuss these results in the context of previous observations that have been made for the roles of Pols eta, iota, and kappa in promoting replication through the minor-groove N2-dG adducts. ..
- Ulrich H. The srs2 suppressor of UV sensitivity acts specifically on the RAD5- and MMS2-dependent branch of the RAD6 pathway. Nucleic Acids Res. 2001;29:3487-94 pubmed..The data presented here support the notion that RAD5, UBC13 and MMS2 encode an ensemble of genetically and physically interacting repair factors within the RAD6 pathway that is coordinately affected by SRS2. ..
- Torres Ramos C, Prakash S, Prakash L. Requirement of RAD5 and MMS2 for postreplication repair of UV-damaged DNA in Saccharomyces cerevisiae. Mol Cell Biol. 2002;22:2419-26 pubmed..Here, we examine the contributions of the REV3, RAD30, RAD5, and MMS2 genes, all of which belong to the RAD6 epistasis group, to the postreplication repair of UV-..
- Qin Z, Lu M, Xu X, Hanna M, Shiomi N, Xiao W. DNA-damage tolerance mediated by PCNA*Ub fusions in human cells is dependent on Rev1 but not Pol?. Nucleic Acids Res. 2013;41:7356-69 pubmed publisher..Hence, PCNA•Ub fusions bypass the requirement for PCNA monoubiquitination, and UV damage tolerance conferred by these fusions is dependent on Rev1 but independent of Pol?...
- Mudrak S, Welz Voegele C, Jinks Robertson S. The polymerase eta translesion synthesis DNA polymerase acts independently of the mismatch repair system to limit mutagenesis caused by 7,8-dihydro-8-oxoguanine in yeast. Mol Cell Biol. 2009;29:5316-26 pubmed publisher..These studies establish a new paradigm for the prevention of GO-associated mutagenesis in eukaryotes. ..
- Yuan F, Zhang Y, Rajpal D, Wu X, Guo D, Wang M, et al. Specificity of DNA lesion bypass by the yeast DNA polymerase eta. J Biol Chem. 2000;275:8233-9 pubmedDNA polymerase eta (Pol(eta), xeroderma pigmentosum variant, or Rad30) plays an important role in an error-free response to unrepaired UV damage during replication...
- Saugar I, Parker J, Zhao S, Ulrich H. The genome maintenance factor Mgs1 is targeted to sites of replication stress by ubiquitylated PCNA. Nucleic Acids Res. 2012;40:245-57 pubmed publisher..Our identification of Mgs1 as a UBZ-dependent downstream effector of ubiquitylated PCNA suggests an explanation for the ambivalent role of the protein in damage processing. ..
- Giannattasio M, Follonier C, Tourrière H, Puddu F, Lazzaro F, Pasero P, et al. Exo1 competes with repair synthesis, converts NER intermediates to long ssDNA gaps, and promotes checkpoint activation. Mol Cell. 2010;40:50-62 pubmed publisher..Our work has significant implications for understanding the coordination between repair of DNA lesions and checkpoint pathways to preserve genome stability. ..
- McIntyre J, Baranowska H, Skoneczna A, Halas A, Sledziewska Gojska E. The spectrum of spontaneous mutations caused by deficiency in proteasome maturase Ump1 in Saccharomyces cerevisiae. Curr Genet. 2007;52:221-8 pubmed..The specificity of the mutator effect caused by ump1 is discussed in light of the proposed role of the proteasome activity in the regulation of the PRR mechanisms. ..
- Karras G, Fumasoni M, Sienski G, Vanoli F, Branzei D, Jentsch S. Noncanonical role of the 9-1-1 clamp in the error-free DNA damage tolerance pathway. Mol Cell. 2013;49:536-46 pubmed publisher..Our findings thus reveal unexpected cooperation in the error-free pathway between the two related clamps and indicate that 9-1-1 plays a broader role in the DNA damage response than previously assumed. ..
- Zamir L, Zaretsky M, Fridman Y, Ner Gaon H, Rubin E, Aharoni A. Tight coevolution of proliferating cell nuclear antigen (PCNA)-partner interaction networks in fungi leads to interspecies network incompatibility. Proc Natl Acad Sci U S A. 2012;109:E406-14 pubmed publisher..Our results indicate that the coevolution of PPI networks can form functional barriers between fungal species, and thus can promote and fix speciation...
- van der Kemp P, de Padula M, Burguiere Slezak G, Ulrich H, Boiteux S. PCNA monoubiquitylation and DNA polymerase eta ubiquitin-binding domain are required to prevent 8-oxoguanine-induced mutagenesis in Saccharomyces cerevisiae. Nucleic Acids Res. 2009;37:2549-59 pubmed publisher..This study suggests that Pol eta and the post-replication repair (PRR) machinery can also prevent mutagenesis at DNA lesions that do not stall replication forks. ..
- Maloisel L, Fabre F, Gangloff S. DNA polymerase delta is preferentially recruited during homologous recombination to promote heteroduplex DNA extension. Mol Cell Biol. 2008;28:1373-82 pubmed..Our results argue strongly for the preferential recruitment of Poldelta during HR. ..
- Martini E, Keeney S, Osley M. A role for histone H2B during repair of UV-induced DNA damage in Saccharomyces cerevisiae. Genetics. 2002;160:1375-87 pubmed..When combined with a ubc13Delta mutation, which is also epistatic with rad5Delta, the htb1-3 mutation enhanced UV-induced cell killing. These results suggest that histone H2B acts in a novel RAD5-dependent branch of PRR. ..
- McIntyre J, Podlaska A, Skoneczna A, Halas A, Sledziewska Gojska E. Analysis of the spontaneous mutator phenotype associated with 20S proteasome deficiency in S. cerevisiae. Mutat Res. 2006;593:153-63 pubmed..Taken together, our findings strongly support the idea that proteolytic activity is involved in modulating the balance between TLS mechanisms functioning during DNA replication in S. cerevisiae. ..
- Cannistraro V, Taylor J. Ability of polymerase eta and T7 DNA polymerase to bypass bulge structures. J Biol Chem. 2007;282:11188-96 pubmed..Terminations were not observed, however, when bulge structures approached the site of interaction of the DNA with the extended thumb and thioredoxin. At low temperature, however, terminations did occur at this site. ..
- Kats E, Enserink J, Martinez S, Kolodner R. The Saccharomyces cerevisiae Rad6 postreplication repair and Siz1/Srs2 homologous recombination-inhibiting pathways process DNA damage that arises in asf1 mutants. Mol Cell Biol. 2009;29:5226-37 pubmed publisher..Our results show that ASF1 probably contributes to the maintenance of genome stability through multiple mechanisms, some of which involve the PRR and HRS pathways...
- Ortiz BazÃ¡n M, Gallo FernÃ¡ndez M, Saugar I, JimÃ©nez MartÃn A, VÃ¡zquez M, Tercero J. Rad5 plays a major role in the cellular response to DNA damage during chromosome replication. Cell Rep. 2014;9:460-8 pubmed publisher..Thus, Rad5 ensures the completion of chromosome replication under DNA-damaging conditions while minimizing the risk of mutagenesis, thereby contributing significantly to genome integrity maintenance. ..
- Carlson K, Washington M. Mechanism of efficient and accurate nucleotide incorporation opposite 7,8-dihydro-8-oxoguanine by Saccharomyces cerevisiae DNA polymerase eta. Mol Cell Biol. 2005;25:2169-76 pubmed..This is mainly due to greater binding affinity for the incorrect incoming dATP opposite 8-oxoG. Overall, these results show that Pol eta replicates through 8-oxoG without any barriers introduced by the presence of the lesion. ..
- Guo X, Jinks Robertson S. Roles of exonucleases and translesion synthesis DNA polymerases during mitotic gap repair in yeast. DNA Repair (Amst). 2013;12:1024-30 pubmed publisher..These polymerases may be involved either in the initiation of DNA synthesis from the an invading end, or in a gap-filling process that is required to complete recombination. ..
- Yang J, Wang R, Liu B, Xue Q, Zhong M, Zeng H, et al. Kinetic analysis of bypass of abasic site by the catalytic core of yeast DNA polymerase eta. Mutat Res. 2015;779:134-43 pubmed publisher..This study provides further understanding in the mutation mechanism of abasic sites for yeast DNA polymerase Î·. ..
- Fan L, Xiao W. The Pol30-K196 residue plays a critical role in budding yeast DNA postreplication repair through interaction with Rad18. DNA Repair (Amst). 2016;47:42-48 pubmed publisher..Together with the Pol30-Ub fusion data, we believe that we have identified a putative Rad18-binding pocket in Pol30 that is required for PCNA monoubiquitination and PRR. ..
- Ang J, Duffy S, Segovia R, Stirling P, Hieter P. Dosage Mutator Genes in Saccharomyces cerevisiae: A Novel Mutator Mode-of-Action of the Mph1 DNA Helicase. Genetics. 2016;204:975-986 pubmed publisher..We believe this represents a novel mutator mode-of-action and opens up new prospects to understand how upregulation of DNA repair proteins may contribute to mutagenesis. ..
- Lee K, Lee S. Saccharomyces cerevisiae Sae2- and Tel1-dependent single-strand DNA formation at DNA break promotes microhomology-mediated end joining. Genetics. 2007;176:2003-14 pubmed..Mre11/Rad50/Xrs2, 3' flap removal by Rad1/Rad10, Nej1, and DNA synthesis by multiple polymerases including Pol4, Rad30, Rev3, and Pol32. The mismatch repair proteins, Rad52 group genes, and Rad27 are dispensable for MMEJ...
- Pessoa Brandao L, Sclafani R. CDC7/DBF4 functions in the translesion synthesis branch of the RAD6 epistasis group in Saccharomyces cerevisiae. Genetics. 2004;167:1597-610 pubmed..have been determined to function in separate branches within this group, including RAD5, REV3/REV7 (Pol zeta), RAD30 (Pol eta), and POL30 (PCNA)...
- Herzberg K, Bashkirov V, Rolfsmeier M, Haghnazari E, McDonald W, Anderson S, et al. Phosphorylation of Rad55 on serines 2, 8, and 14 is required for efficient homologous recombination in the recovery of stalled replication forks. Mol Cell Biol. 2006;26:8396-409 pubmed..These results suggest that Rad55-S2,8,14 phosphorylation activates recombinational repair, allowing for faster recovery after genotoxic stress. ..
- Liu B, Xue Q, Gu S, Wang W, Chen J, Li Y, et al. Kinetic analysis of bypass of O(6)- methylguanine by the catalytic core of yeast DNA polymerase eta. Arch Biochem Biophys. 2016;596:99-107 pubmed publisher..This study provides further understanding of the mutation mechanism of alkylated lesion for yeast DNA polymerase Î·. ..
- Lehner K, Jinks Robertson S. Shared genetic pathways contribute to the tolerance of endogenous and low-dose exogenous DNA damage in yeast. Genetics. 2014;198:519-30 pubmed publisher..These results have important implications when considering what constitutes a safe and acceptable level of exogenous DNA damage. ..
- Hoffman P, Curtis M, Iwai S, Hays J. Biochemical evolution of DNA polymerase eta: properties of plant, human, and yeast proteins. Biochemistry. 2008;47:4583-96 pubmed publisher..The depressed ( N - 1) insertion upstream of T[CPD]T (but not T-T) may reduce the extent of gratuitous error-prone insertion. ..
- Daraba A, Gali V, Halmai M, Haracska L, Unk I. Def1 promotes the degradation of Pol3 for polymerase exchange to occur during DNA-damage--induced mutagenesis in Saccharomyces cerevisiae. PLoS Biol. 2014;12:e1001771 pubmed publisher..Our results imply that TLS polymerases carry out DNA lesion bypass only after the Def1-assisted removal of Pol3 from the stalled replication fork...
- Abdulovic A, Minesinger B, Jinks Robertson S. Identification of a strand-related bias in the PCNA-mediated bypass of spontaneous lesions by yeast Poleta. DNA Repair (Amst). 2007;6:1307-18 pubmed..Our results suggest that there is a polymerase hierarchy between Poleta and Polzeta in the bypass of certain lesions and that the interaction of Poleta with PCNA is needed for some, but not all, spontaneous lesion bypass. ..
- Boehm E, Spies M, Washington M. PCNA tool belts and polymerase bridges form during translesion synthesis. Nucleic Acids Res. 2016;44:8250-60 pubmed publisher..The formation of PCNA tool belts and Rev1 bridges and the ability of these complexes to change architectures are likely means of facilitating selection of the appropriate non-classical polymerase and polymerase-switching events. ..
- O Brien T, Witcher P, Brooks B, Patierno S. DNA polymerase zeta is essential for hexavalent chromium-induced mutagenesis. Mutat Res. 2009;663:77-83 pubmed publisher..Wild-type yeast and strains deficient in TLS polymerases (i.e. Polzeta (rev3), Poleta (rad30)) were exposed to Cr(VI) and monitored for cell survival and forward mutagenesis at the CAN1 locus...
- Bostelman L, Keller A, Albrecht A, Arat A, Thompson J. Methylation of histone H3 lysine-79 by Dot1p plays multiple roles in the response to UV damage in Saccharomyces cerevisiae. DNA Repair (Amst). 2007;6:383-95 pubmed..The overall results indicate the existence of distinct and separable roles of histone H3 lysine-79 methylation in the response to UV damage, potentially serving to coordinate the various repair processes. ..
- Erlich R, Fry R, Begley T, Daee D, Lahue R, Samson L. Anc1, a protein associated with multiple transcription complexes, is involved in postreplication repair pathway in S. cerevisiae. PLoS ONE. 2008;3:e3717 pubmed publisher..Anc1's role in the PRR pathway, as well as its role in suppressing triplet repeats, point to a possible mechanism for a protein of potential medical interest. ..
- Monti P, Broxson C, Inga A, Wang R, Menichini P, Tornaletti S, et al. 3-Methyl-3-deazaadenine, a stable isostere of N3-methyl-adenine, is efficiently bypassed by replication in vivo and by transcription in vitro. DNA Repair (Amst). 2011;10:861-8 pubmed publisher..the AP-site dramatically reduced plasmid replication in all strains, the 3-m-c(3)A had a slight effect in the rad30 background which significantly increased only in a rev3rad30 background...
- Chen Y, Sugiyama T. NGS-based analysis of base-substitution signatures created by yeast DNA polymerase eta and zeta on undamaged and abasic DNA templates in vitro. DNA Repair (Amst). 2017;59:34-43 pubmed publisher..Polζ and Polη cooperatively facilitated the TLS reaction over an abasic site in vitro, suggesting that these two polymerases can cooperate in efficient and high fidelity TLS. ..
- Boehm E, Powers K, Kondratick C, Spies M, Houtman J, Washington M. The Proliferating Cell Nuclear Antigen (PCNA)-interacting Protein (PIP) Motif of DNA Polymerase Î· Mediates Its Interaction with the C-terminal Domain of Rev1. J Biol Chem. 2016;291:8735-44 pubmed publisher..These findings also suggest that PIP motifs are a more versatile protein interaction motif than previously believed. ..
- Plachta M, Halas A, McIntyre J, Sledziewska Gojska E. The steady-state level and stability of TLS polymerase eta are cell cycle dependent in the yeast S. cerevisiae. DNA Repair (Amst). 2015;29:147-53 pubmed publisher..Our results, in concert with previous data for Rev1, indicate that cell cycle regulation is a general property of Y family TLS polymerases in S. cerevisiae. ..
- Ma W, Westmoreland J, Resnick M. Homologous recombination rescues ssDNA gaps generated by nucleotide excision repair and reduced translesion DNA synthesis in yeast G2 cells. Proc Natl Acad Sci U S A. 2013;110:E2895-904 pubmed publisher..In contrast to WT cells, there was a dramatic increase in ssDNA gaps for cells deficient in the TLS polymerases ? (Rad30) and ? (Rev3)...
- Chatterjee N, Pabla R, Siede W. Role of polymerase ? in mitochondrial mutagenesis of Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2013;431:270-3 pubmed publisher..For base pair substitutions, polymerase ? appears to be related in function and epistatic to DNA polymerase ? which, however, plays the opposite role in the nucleus. ..
- Haruta N, Kubota Y, Hishida T. Chronic low-dose ultraviolet-induced mutagenesis in nucleotide excision repair-deficient cells. Nucleic Acids Res. 2012;40:8406-15 pubmed..C?T mutations in rad14? cells are dependent on translesion synthesis (TLS) DNA polymerase ?, encoded by RAD30, despite its previously established role in error-free TLS...
- McCulloch S, Wood A, Garg P, Burgers P, Kunkel T. Effects of accessory proteins on the bypass of a cis-syn thymine-thymine dimer by Saccharomyces cerevisiae DNA polymerase eta. Biochemistry. 2007;46:8888-96 pubmed..Thus, although accessory proteins clearly participate in pol eta functions in vivo, they do not appear to help suppress UV mutagenesis by improving pol eta bypass fidelity per se. ..