RAD27

Summary

Gene Symbol: RAD27
Description: multifunctional nuclease RAD27
Alias: ERC11, FEN1, RTH1, multifunctional nuclease RAD27
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Parenteau J, Wellinger R. Accumulation of single-stranded DNA and destabilization of telomeric repeats in yeast mutant strains carrying a deletion of RAD27. Mol Cell Biol. 1999;19:4143-52 pubmed
    The Saccharomyces cerevisiae RAD27 gene encodes the yeast homologue of the mammalian FEN-1 nuclease, a protein that is thought to be involved in the processing of Okazaki fragments during DNA lagging-strand synthesis...
  2. Klein H. Mutations in recombinational repair and in checkpoint control genes suppress the lethal combination of srs2Delta with other DNA repair genes in Saccharomyces cerevisiae. Genetics. 2001;157:557-65 pubmed
    ..However, cells do not achieve wild-type growth rates, suggesting that unrepaired damage is still present and may lead to chromosome loss. ..
  3. Wu X, Wang Z. Relationships between yeast Rad27 and Apn1 in response to apurinic/apyrimidinic (AP) sites in DNA. Nucleic Acids Res. 1999;27:956-62 pubmed
    Yeast Rad27 is a 5'-->3' exonuclease and a flap endo-nuclease. Apn1 is the major apurinic/apyrimidinic (AP) endonuclease in yeast. The rad27 deletion mutants are highly sensitive to methylmethane sulfonate (MMS)...
  4. Tseng H, Tomkinson A. Processing and joining of DNA ends coordinated by interactions among Dnl4/Lif1, Pol4, and FEN-1. J Biol Chem. 2004;279:47580-8 pubmed
    ..Saccharomyces cerevisiae have implicated the DNA polymerase Pol4 and the DNA structure-specific endonuclease FEN-1(Rad27) in the processing of DNA ends to be joined by Dnl4/Lif1...
  5. Munashingha P, Lee C, Kang Y, Shin Y, Nguyen T, Seo Y. The trans-autostimulatory activity of Rad27 suppresses dna2 defects in Okazaki fragment processing. J Biol Chem. 2012;287:8675-87 pubmed publisher
    Dna2 and Rad27 (yeast Fen1) are the two endonucleases critical for Okazaki fragment processing during lagging strand DNA synthesis that have been shown to interact genetically and physically...
  6. Kao H, Henricksen L, Liu Y, Bambara R. Cleavage specificity of Saccharomyces cerevisiae flap endonuclease 1 suggests a double-flap structure as the cellular substrate. J Biol Chem. 2002;277:14379-89 pubmed
    Flap endonuclease 1 (FEN1) is a structure-specific nuclease that cleaves substrates containing unannealed 5'-flaps during Okazaki fragment processing. Cleavage removes the flap at or near the point of annealing...
  7. Tishkoff D, Filosi N, Gaida G, Kolodner R. A novel mutation avoidance mechanism dependent on S. cerevisiae RAD27 is distinct from DNA mismatch repair. Cell. 1997;88:253-63 pubmed
    Mutations in the S. cerevisiae RAD27 (also called RTH1 or YKL510) gene result in a strong mutator phenotype...
  8. Debrauwere H, Loeillet S, Lin W, Lopes J, Nicolas A. Links between replication and recombination in Saccharomyces cerevisiae: a hypersensitive requirement for homologous recombination in the absence of Rad27 activity. Proc Natl Acad Sci U S A. 2001;98:8263-9 pubmed
    The RAD27 gene of Saccharomyces cerevisiae encodes a 5'-3' flap exo/endonuclease, which plays an important role during DNA replication for Okazaki fragment maturation...
  9. Ma W, Panduri V, Sterling J, Van Houten B, Gordenin D, Resnick M. The transition of closely opposed lesions to double-strand breaks during long-patch base excision repair is prevented by the coordinated action of DNA polymerase delta and Rad27/Fen1. Mol Cell Biol. 2009;29:1212-21 pubmed publisher
    ..cerevisiae (budding yeast) that impede the interaction of DNA polymerase delta and the 5'-flap endonuclease Rad27/Fen1 with the PCNA sliding clamp, we show that the lack of coordination between these components during long-patch ..

More Information

Publications78

  1. Gellon L, Carson D, Carson J, Demple B. Intrinsic 5'-deoxyribose-5-phosphate lyase activity in Saccharomyces cerevisiae Trf4 protein with a possible role in base excision DNA repair. DNA Repair (Amst). 2008;7:187-98 pubmed
    ..Only a RAD27 genetic interaction was detected: there was higher sensitivity for strains mutated in both TRF4 and RAD27 than ..
  2. Kang M, Lee C, Kang Y, Cho I, Nguyen T, Seo Y. Genetic and functional interactions between Mus81-Mms4 and Rad27. Nucleic Acids Res. 2010;38:7611-25 pubmed publisher
    The two endonucleases, Rad27 (yeast Fen1) and Dna2, jointly participate in the processing of Okazaki fragments in yeasts...
  3. Gary R, Park M, Nolan J, Cornelius H, Kozyreva O, Tran H, et al. A novel role in DNA metabolism for the binding of Fen1/Rad27 to PCNA and implications for genetic risk. Mol Cell Biol. 1999;19:5373-82 pubmed
    Fen1/Rad27 nuclease activity, which is important in DNA metabolism, is stimulated by proliferating cell nuclear antigen (PCNA) in vitro. The in vivo role of the PCNA interaction was investigated in the yeast Rad27...
  4. Kim J, Kang Y, Kang H, Kim D, Ryu G, Kang M, et al. In vivo and in vitro studies of Mgs1 suggest a link between genome instability and Okazaki fragment processing. Nucleic Acids Res. 2005;33:6137-50 pubmed
    ..Mgs1 stimulates the structure-specific nuclease activity of Rad27 (yeast Fen1 or yFen1) in an ATP-dependent manner...
  5. Jin Y, Ayyagari R, Resnick M, Gordenin D, Burgers P. Okazaki fragment maturation in yeast. II. Cooperation between the polymerase and 3'-5'-exonuclease activities of Pol delta in the creation of a ligatable nick. J Biol Chem. 2003;278:1626-33 pubmed
    To address the different functions of Pol delta and FEN1 (Rad27) in Okazaki fragment maturation, exonuclease-deficient polymerase Pol delta-01 and Pol delta-5DV (corresponding to alleles pol3-01-(D321A, E323A) and pol3-5DV-(D520V), ..
  6. Symington L. Homologous recombination is required for the viability of rad27 mutants. Nucleic Acids Res. 1998;26:5589-95 pubmed
    The RAD27/RTH1 gene of Saccharomyces cerevisiae encodes a structural and functional homolog of the 5'-3' exonuclease function of Escherichia coli DNA polymerase I...
  7. Formosa T, Nittis T. Dna2 mutants reveal interactions with Dna polymerase alpha and Ctf4, a Pol alpha accessory factor, and show that full Dna2 helicase activity is not essential for growth. Genetics. 1999;151:1459-70 pubmed
    ..Dna2 therefore appears to act in repair or lagging strand synthesis together with Pol alpha and Ctf4, in a role that is optimal with, but does not require, full helicase activity. ..
  8. Navarro M, Bi L, Bailis A. A mutant allele of the transcription factor IIH helicase gene, RAD3, promotes loss of heterozygosity in response to a DNA replication defect in Saccharomyces cerevisiae. Genetics. 2007;176:1391-402 pubmed
    ..In the yeast Saccharomyces cerevisiae, a null allele of the RAD27 gene, which encodes a structure-specific nuclease involved in Okazaki fragment processing, stimulates mutation and ..
  9. Vallen E, Cross F. Mutations in RAD27 define a potential link between G1 cyclins and DNA replication. Mol Cell Biol. 1995;15:4291-302 pubmed
    ..We have isolated alleles of RAD27/ERC11/YKL510, the yeast homolog of the gene encoding flap endonuclease 1, FEN-1...
  10. Budd M, Campbell J. A yeast replicative helicase, Dna2 helicase, interacts with yeast FEN-1 nuclease in carrying out its essential function. Mol Cell Biol. 1997;17:2136-42 pubmed
    ..Overproduction of yFEN-1, encoded by RAD27/RTH1, suppresses the temperature-sensitive growth of dna2-1 mutants...
  11. Daley J, Wilson T, Ramotar D. Genetic interactions between HNT3/Aprataxin and RAD27/FEN1 suggest parallel pathways for 5' end processing during base excision repair. DNA Repair (Amst). 2010;9:690-9 pubmed publisher
    ..Surprisingly, HNT3 deletion partially rescued H(2)O(2) sensitivity in recombination-deficient rad51Delta and rad52Delta cells, suggesting that Hnt3 promotes formation of a repair intermediate that is resolved by recombination. ..
  12. Jimeno S, Herrera Moyano E, Ortega P, Aguilera A. Differential effect of the overexpression of Rad2/XPG family endonucleases on genome integrity in yeast and human cells. DNA Repair (Amst). 2017;57:66-75 pubmed publisher
    ..Rad2/XPG and Rad27/FEN1 belong to a group of evolutionary conserved proteins that constitute the Rad2 family...
  13. Kanellis P, Agyei R, Durocher D. Elg1 forms an alternative PCNA-interacting RFC complex required to maintain genome stability. Curr Biol. 2003;13:1583-95 pubmed
    ..Elg1 interacts physically with the PCNA homolog Pol30 and the FEN-1 homolog Rad27. The physical and genetic interactions suggest a role for Elg1 in Okazaki fragment maturation...
  14. Kirchner J, Tran H, Resnick M. A DNA polymerase epsilon mutant that specifically causes +1 frameshift mutations within homonucleotide runs in yeast. Genetics. 2000;155:1623-32 pubmed
    ..When pol2-C1089Y was combined with deletions of EXO1 or RAD27 (homologue of human FEN1), mutation rates were increased for +1 frameshifts while there was almost no effect on -1 ..
  15. Piening B, Huang D, Paulovich A. Novel connections between DNA replication, telomere homeostasis, and the DNA damage response revealed by a genome-wide screen for TEL1/ATM interactions in Saccharomyces cerevisiae. Genetics. 2013;193:1117-33 pubmed publisher
  16. Nguyen T, Tak Y, Lee C, Kang Y, Cho I, Seo Y. Analysis of subunit assembly and function of the Saccharomyces cerevisiae RNase H2 complex. FEBS J. 2011;278:4927-42 pubmed publisher
    ..We found that the substrate-binding activities of both Rnh201 and Rnh202 were critical for cleavage of the phosphodiester bond present between DNA and RNA in RNase H2 substrates. ..
  17. Allen Soltero S, Martinez S, Putnam C, Kolodner R. A saccharomyces cerevisiae RNase H2 interaction network functions to suppress genome instability. Mol Cell Biol. 2014;34:1521-34 pubmed publisher
    ..This analysis suggests that cells with RNase H2 defects have increased levels of DNA damage and depend on other pathways of DNA metabolism to overcome the deleterious effects of this DNA damage. ..
  18. Moreau S, Morgan E, Symington L. Overlapping functions of the Saccharomyces cerevisiae Mre11, Exo1 and Rad27 nucleases in DNA metabolism. Genetics. 2001;159:1423-33 pubmed
    ..Deletion of RAD27, which encodes a flap endonuclease, causes inviability in mre11 strains...
  19. Tran P, Erdeniz N, Dudley S, Liskay R. Characterization of nuclease-dependent functions of Exo1p in Saccharomyces cerevisiae. DNA Repair (Amst). 2002;1:895-912 pubmed
    ..Finally, our findings plus those of other investigators suggest that Exo1p functions both in a catalytic and a structural capacity during DNA MMR. ..
  20. Kokoska R, Stefanovic L, Tran H, Resnick M, Gordenin D, Petes T. Destabilization of yeast micro- and minisatellite DNA sequences by mutations affecting a nuclease involved in Okazaki fragment processing (rad27) and DNA polymerase delta (pol3-t). Mol Cell Biol. 1998;18:2779-88 pubmed
    We examined the effects of mutations in the Saccharomyces cerevisiae RAD27 (encoding a nuclease involved in the processing of Okazaki fragments) and POL3 (encoding DNA polymerase delta) genes on the stability of a minisatellite sequence (..
  21. Yimit A, Riffle M, Brown G. Genetic Regulation of Dna2 Localization During the DNA Damage Response. G3 (Bethesda). 2015;5:1937-44 pubmed publisher
    ..We identified 37 gene deletions that affect Dna2-GFP focus formation, 12 with fewer foci and 25 with increased foci. Together these data comprise a useful resource for understanding Dna2 regulation in response to DNA damage. ..
  22. Kolesar P, Altmannova V, Silva S, Lisby M, Krejci L. Pro-recombination Role of Srs2 Protein Requires SUMO (Small Ubiquitin-like Modifier) but Is Independent of PCNA (Proliferating Cell Nuclear Antigen) Interaction. J Biol Chem. 2016;291:7594-607 pubmed publisher
    ..Notably, sumoylation of Srs2 itself specifically stimulates recombination at the rDNA locus. ..
  23. Ward T, McHugh P, Durant S. Small molecule inhibitors uncover synthetic genetic interactions of human flap endonuclease 1 (FEN1) with DNA damage response genes. PLoS ONE. 2017;12:e0179278 pubmed publisher
    Flap endonuclease 1 (FEN1) is a structure selective endonuclease required for proficient DNA replication and the repair of DNA damage...
  24. Hang L, Lopez C, Liu X, Williams J, Chung I, Wei L, et al. Regulation of Ku-DNA association by Yku70 C-terminal tail and SUMO modification. J Biol Chem. 2014;289:10308-17 pubmed publisher
    ..These results suggest a model whereby Yku70 sumoylation upon DNA association strengthens Ku-DNA interaction to promote multiple functions of Ku. ..
  25. Kadyrova L, Dahal B, Kadyrov F. Evidence that the DNA mismatch repair system removes 1-nucleotide Okazaki fragment flaps. J Biol Chem. 2015;290:24051-65 pubmed publisher
    ..We found that in the yeast Saccharomyces cerevisiae, the MMR system and the flap endonuclease Rad27 act in overlapping pathways that protect the nuclear genome from 1-bp insertions...
  26. Mimitou E, Symington L. Ku prevents Exo1 and Sgs1-dependent resection of DNA ends in the absence of a functional MRX complex or Sae2. EMBO J. 2010;29:3358-69 pubmed publisher
    ..Finally, we show that the presence of Ku exacerbates DNA end-processing defects established in the sae2? sgs1? mutant, leading to its lethality. ..
  27. Budd M, Campbell J. The pattern of sensitivity of yeast dna2 mutants to DNA damaging agents suggests a role in DSB and postreplication repair pathways. Mutat Res. 2000;459:173-86 pubmed
    ..by overexpression of a 5' to 3' exonuclease, the yeast FEN-1 structure-specific nuclease, encoded by the RAD27 gene, which also suppresses the growth defect of dna2-ts mutants...
  28. Refsland E, Livingston D. Interactions among DNA ligase I, the flap endonuclease and proliferating cell nuclear antigen in the expansion and contraction of CAG repeat tracts in yeast. Genetics. 2005;171:923-34 pubmed
    Among replication mutations that destabilize CAG repeat tracts, mutations of RAD27, encoding the flap endonuclease, and CDC9, encoding DNA ligase I, increase the incidence of repeat tract expansions to the greatest extent...
  29. Yang H, Matsumoto Y, Trujillo K, Lees Miller S, Osley M, Tomkinson A. Role of the yeast DNA repair protein Nej1 in end processing during the repair of DNA double strand breaks by non-homologous end joining. DNA Repair (Amst). 2015;31:1-10 pubmed publisher
    ..Although the yeast proteins, Pol4, a Pol X family DNA polymerase, and Rad27, a nuclease, participate in the end processing reactions of NHEJ, the mechanisms underlying the recruitment of ..
  30. Chin J, Bashkirov V, Heyer W, Romesberg F. Esc4/Rtt107 and the control of recombination during replication. DNA Repair (Amst). 2006;5:618-28 pubmed
    ..Thus, we propose that Esc4 associates with ssDNA of stalled forks and acts as a scaffolding protein to recruit and/or modulate the function of other proteins required to reinitiate DNA synthesis. ..
  31. Zamir L, Zaretsky M, Fridman Y, Ner Gaon H, Rubin E, Aharoni A. Tight coevolution of proliferating cell nuclear antigen (PCNA)-partner interaction networks in fungi leads to interspecies network incompatibility. Proc Natl Acad Sci U S A. 2012;109:E406-14 pubmed publisher
    ..Our results indicate that the coevolution of PPI networks can form functional barriers between fungal species, and thus can promote and fix speciation...
  32. Woolstencroft R, Beilharz T, Cook M, Preiss T, Durocher D, Tyers M. Ccr4 contributes to tolerance of replication stress through control of CRT1 mRNA poly(A) tail length. J Cell Sci. 2006;119:5178-92 pubmed
    ..These results implicate the coordinated regulation of Crt1 via Ccr4 and Dun1 as a crucial nodal point in the response to DNA replication stress. ..
  33. Soustelle C, Vernis L, Fréon K, Reynaud Angelin A, Chanet R, Fabre F, et al. A new Saccharomyces cerevisiae strain with a mutant Smt3-deconjugating Ulp1 protein is affected in DNA replication and requires Srs2 and homologous recombination for its viability. Mol Cell Biol. 2004;24:5130-43 pubmed
    ..These structures are believed to generate different recombination intermediates. Some of them are fixed by recombination, and others require Srs2 to be reversed and fixed by an alternate pathway. ..
  34. Rossi M, Bambara R. Reconstituted Okazaki fragment processing indicates two pathways of primer removal. J Biol Chem. 2006;281:26051-61 pubmed
    ..replication protein A (RPA), and sequential cleavage of the flap by Dna2 nuclease followed by flap endonuclease 1 (FEN1)...
  35. Holmes A, Haber J. Double-strand break repair in yeast requires both leading and lagging strand DNA polymerases. Cell. 1999;96:415-24 pubmed
    ..mutants of lagging strand replication, DNA polymerase alpha (pol1-17), DNA primase (pri2-1), and Rad27p (rad27 delta) also greatly inhibit completion of DSB repair, even in G1-arrested cells...
  36. Heacock M, Poltoratsky V, Prasad R, Wilson S. Evidence for abasic site sugar phosphate-mediated cytotoxicity in alkylating agent treated Saccharomyces cerevisiae. PLoS ONE. 2012;7:e47945 pubmed publisher
    ..agent-induced cytotoxicity and the base lesion DNA repair process in Saccharomyces cerevisiae, we replaced the RAD27(FEN1) open reading frame (ORF) with the ORF of the bifunctional human repair enzyme DNA polymerase (Pol) ?...
  37. Keogh M, Kim J, Downey M, Fillingham J, Chowdhury D, Harrison J, et al. A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery. Nature. 2006;439:497-501 pubmed
    ..The dephosphorylation of gammaH2AX by the HTP-C is necessary for efficient recovery from the DNA damage checkpoint. ..
  38. Jin Y, Obert R, Burgers P, Kunkel T, Resnick M, Gordenin D. The 3'-->5' exonuclease of DNA polymerase delta can substitute for the 5' flap endonuclease Rad27/Fen1 in processing Okazaki fragments and preventing genome instability. Proc Natl Acad Sci U S A. 2001;98:5122-7 pubmed
    ..We describe a role of the 3'-->5' Exo of Pol delta as a supplement or backup for the Rad27/Fen1 5' flap endonuclease...
  39. Thu H, Nguyen T, Munashingha P, Kwon B, Dao Van Q, Seo Y. A physiological significance of the functional interaction between Mus81 and Rad27 in homologous recombination repair. Nucleic Acids Res. 2015;43:1684-99 pubmed publisher
    ..Here, we show the in vivo significance of the interactions between Mus81 and Rad27 (yeast Fen1)...
  40. Kaniak Golik A, Kuberska R, Dzierzbicki P, Sledziewska Gojska E. Activation of Dun1 in response to nuclear DNA instability accounts for the increase in mitochondrial point mutations in Rad27/FEN1 deficient S. cerevisiae. PLoS ONE. 2017;12:e0180153 pubmed publisher
    b>Rad27/FEN1 nuclease that plays important roles in the maintenance of DNA stability in the nucleus has recently been shown to reside in mitochondria...
  41. Kumar C, Piacente S, Sibert J, Bukata A, O Connor J, Alani E, et al. Multiple factors insulate Msh2-Msh6 mismatch repair activity from defects in Msh2 domain I. J Mol Biol. 2011;411:765-80 pubmed publisher
    ..with mutations in two distinct regions of MSH6 and those that increase cellular mutational load (pol3-01 and rad27)...
  42. Karanja K, Livingston D. C-terminal flap endonuclease (rad27) mutations: lethal interactions with a DNA ligase I mutation (cdc9-p) and suppression by proliferating cell nuclear antigen (POL30) in Saccharomyces cerevisiae. Genetics. 2009;183:63-78 pubmed publisher
    ..We recovered the flap endonuclease mutation rad27-K325* with a stop codon at residue 325...
  43. KALIFA L, Beutner G, Phadnis N, Sheu S, Sia E. Evidence for a role of FEN1 in maintaining mitochondrial DNA integrity. DNA Repair (Amst). 2009;8:1242-9 pubmed publisher
    ..In this study, we analyzed the effects of deleting the yeast FEN1 homolog, RAD27, on mtDNA stability in Saccharomyces cerevisiae...
  44. Stewart J, Campbell J, Bambara R. Significance of the dissociation of Dna2 by flap endonuclease 1 to Okazaki fragment processing in Saccharomyces cerevisiae. J Biol Chem. 2009;284:8283-91 pubmed publisher
    ..Flap endonuclease 1 (FEN1) and Dna2 are responsible for flap cleavage...
  45. Henry R, Balakrishnan L, Ying Lin S, Campbell J, Bambara R. Components of the secondary pathway stimulate the primary pathway of eukaryotic Okazaki fragment processing. J Biol Chem. 2010;285:28496-505 pubmed publisher
    ..In most cases, FEN1 (flap endonuclease 1) is able to efficiently cleave short flaps as they form...
  46. Li L, Murphy K, Kanevets U, Reha Krantz L. Sensitivity to phosphonoacetic acid: a new phenotype to probe DNA polymerase delta in Saccharomyces cerevisiae. Genetics. 2005;170:569-80 pubmed
    ..Our initial studies reveal that replication by the L612M-DNA pol delta requires Rad27 flap endonuclease activity since the pol3-L612M strain is not viable in the absence of RAD27 function...
  47. Godin S, Zhang Z, Herken B, Westmoreland J, Lee A, Mihalevic M, et al. The Shu complex promotes error-free tolerance of alkylation-induced base excision repair products. Nucleic Acids Res. 2016;44:8199-215 pubmed publisher
    ..Together, our work demonstrates that the Shu complex's promotion of Rad51 pre-synaptic filaments is critical for high-fidelity bypass of multiple replication-blocking lesion. ..
  48. Hoopes L, Budd M, Choe W, Weitao T, Campbell J. Mutations in DNA replication genes reduce yeast life span. Mol Cell Biol. 2002;22:4136-46 pubmed
    ..Several other replication mutations, such as rad27 Delta, encoding the FEN-1 nuclease involved in several aspects of genomic stability, also show premature aging...
  49. Kelly M, Brosnan L, Jauert P, Dunham M, Kirkpatrick D. Multiple pathways regulate minisatellite stability during stationary phase in yeast. G3 (Bethesda). 2012;2:1185-95 pubmed publisher
    ..In this work, we identified mutants of END3, PKC1, and RAD27 that increase minisatellite instability during stationary phase...
  50. Paulovich A, Armour C, Hartwell L. The Saccharomyces cerevisiae RAD9, RAD17, RAD24 and MEC3 genes are required for tolerating irreparable, ultraviolet-induced DNA damage. Genetics. 1998;150:75-93 pubmed
    ..Therefore, checkpoint genes not only control cell cycle progression in response to damage, but also play a role in accommodating DNA damage during replication. ..
  51. Banerjee S, Myung K. Increased genome instability and telomere length in the elg1-deficient Saccharomyces cerevisiae mutant are regulated by S-phase checkpoints. Eukaryot Cell. 2004;3:1557-66 pubmed
  52. Jin Y, Garg P, Stith C, Al Refai H, Sterling J, Murray L, et al. The multiple biological roles of the 3'-->5' exonuclease of Saccharomyces cerevisiae DNA polymerase delta require switching between the polymerase and exonuclease domains. Mol Cell Biol. 2005;25:461-71 pubmed
    ..Mutation rates and spectra and synergistic interactions of the pol3-L523X mutations with msh2, exo1, and rad27/fen1 defects were indistinguishable from those observed with previously studied exonuclease-defective mutants of ..
  53. Liddell L, Manthey G, Owens S, Fu B, Bailis A. Alleles of the homologous recombination gene, RAD59, identify multiple responses to disrupted DNA replication in Saccharomyces cerevisiae. BMC Microbiol. 2013;13:229 pubmed publisher
    ..Rad59 is required for multiple homologous recombination mechanisms and viability in DNA replication-defective rad27 mutant cells...
  54. Zhu F, Biswas E, Biswas S. Purification and characterization of the DNA polymerase alpha associated exonuclease: the RTH1 gene product. Biochemistry. 1997;36:5947-54 pubmed
    ..cerevisiae gene YKL510 encoded polypeptide, which is also known as yeast RAD2 homolog 1 or RTH1 nuclease...
  55. Chen X, Silver H, Xiong L, Belichenko I, Adegite C, Johnson E. Topoisomerase I-dependent viability loss in saccharomyces cerevisiae mutants defective in both SUMO conjugation and DNA repair. Genetics. 2007;177:17-30 pubmed
    ..siz2Delta mutants fail to grow in the absence of the homologous recombination pathway or the Fen1 ortholog RAD27. Remarkably, the growth defects of mutants such as siz1Delta siz2Delta rad52Delta are suppressed by mutations in ..
  56. Foster S, Balestrini A, Petrini J. Functional interplay of the Mre11 nuclease and Ku in the response to replication-associated DNA damage. Mol Cell Biol. 2011;31:4379-89 pubmed publisher
    ..Collectively, the data define a nonhomologous end joining (NHEJ)-independent, S-phase-specific function of the Ku heterodimer. ..
  57. Rudra S, Skibbens R. Sister chromatid cohesion establishment occurs in concert with lagging strand synthesis. Cell Cycle. 2012;11:2114-21 pubmed publisher
    ..Our genetic and biochemical studies link Ctf7/Eco1 to the Okazaki fragment flap endonuclease, Fen1. We further report genetic and biochemical interactions between Fen1 and the cohesion-associated DNA helicase, Chl1...
  58. Koren A, Soifer I, Barkai N. MRC1-dependent scaling of the budding yeast DNA replication timing program. Genome Res. 2010;20:781-90 pubmed publisher
    ..Mrc1 emerges as a regulator of this robustness of the replication program. ..
  59. Hwang J, Smith S, Myung K. The Rad1-Rad10 complex promotes the production of gross chromosomal rearrangements from spontaneous DNA damage in Saccharomyces cerevisiae. Genetics. 2005;169:1927-37 pubmed
    ..Results presented here suggest that Rad1-Rad10 functions at different stages of GCR formation and that there is an alternative pathway for the GCR formation that is independent of Rad1-Rad10. ..
  60. Subramanian J, Vijayakumar S, Tomkinson A, Arnheim N. Genetic instability induced by overexpression of DNA ligase I in budding yeast. Genetics. 2005;171:427-41 pubmed
    ..Furthermore, in vitro analysis indicates that the interaction of PCNA with Cdc9p and Rad27p (Fen1) is mutually exclusive...
  61. Xie Y, Counter C, Alani E. Characterization of the repeat-tract instability and mutator phenotypes conferred by a Tn3 insertion in RFC1, the large subunit of the yeast clamp loader. Genetics. 1999;151:499-509 pubmed
    ..This analysis indicated that the rfc1::Tn3 allele displays synthetic lethality with pol30, pol3, and rad27 mutations...
  62. McInnis M, O Neill G, Fossum K, Reagan M. Epistatic analysis of the roles of the RAD27 and POL4 gene products in DNA base excision repair in S. cerevisiae. DNA Repair (Amst). 2002;1:311-5 pubmed
    ..We have used an epistasis analysis to investigate whether the proteins encoded by the POL4 and RAD27 genes participate in alternative, non-redundant subpathways of DNA base excision repair (BER)...
  63. Ang J, Duffy S, Segovia R, Stirling P, Hieter P. Dosage Mutator Genes in Saccharomyces cerevisiae: A Novel Mutator Mode-of-Action of the Mph1 DNA Helicase. Genetics. 2016;204:975-986 pubmed publisher
    ..by MPH1 overexpression was independent of translesion synthesis (TLS), but was suppressed by overexpression of RAD27, a conserved flap endonuclease...
  64. Sun X, Thrower D, Qiu J, Wu P, Zheng L, Zhou M, et al. Complementary functions of the Saccharomyces cerevisiae Rad2 family nucleases in Okazaki fragment maturation, mutation avoidance, and chromosome stability. DNA Repair (Amst). 2003;2:925-40 pubmed
    ..Three members of the Saccharomyces cerevisiae Rad2 family, Rad2, Rad27, and exonuclease 1 (Exo1), exhibit both 5' exonuclease and flap endonuclease activities...
  65. Palancade B, Liu X, Garcia Rubio M, Aguilera A, Zhao X, Doye V. Nucleoporins prevent DNA damage accumulation by modulating Ulp1-dependent sumoylation processes. Mol Biol Cell. 2007;18:2912-23 pubmed
    ..Our results thus provide a molecular mechanism that underlies the connection between NPC and genome stability. ..
  66. Kwon B, Munashingha P, Shin Y, Lee C, Li B, Seo Y. Physical and functional interactions between nucleosomes and Rad27, a critical component of DNA processing during DNA metabolism. FEBS J. 2016;283:4247-4262 pubmed publisher
    ..In this report, we present evidence that the nucleosomes can positively regulate the enzymatic activity of Rad27 (yeast Fen1), a major processing enzyme important for Okazaki fragment in eukaryotes...
  67. Daee D, Mertz T, Lahue R. Postreplication repair inhibits CAG.CTG repeat expansions in Saccharomyces cerevisiae. Mol Cell Biol. 2007;27:102-10 pubmed
    ..We also investigated the relationship between PRR and Rad27 (Fen1), a well-established inhibitor of TNR expansions that acts at 5' flaps...
  68. Barber L, Ward T, Hartley J, McHugh P. DNA interstrand cross-link repair in the Saccharomyces cerevisiae cell cycle: overlapping roles for PSO2 (SNM1) with MutS factors and EXO1 during S phase. Mol Cell Biol. 2005;25:2297-309 pubmed
    ..These findings have led to considerable clarification of the complex genetic relationship between various ICL repair pathways. ..
  69. Sommer D, Stith C, Burgers P, Lahue R. Partial reconstitution of DNA large loop repair with purified proteins from Saccharomyces cerevisiae. Nucleic Acids Res. 2008;36:4699-707 pubmed publisher
    ..In the current study, biochemical fractionation studies identified FEN1 (Rad27) as another required LLR component...