RAD23

Summary

Gene Symbol: RAD23
Description: Rad23p
Alias: Rad23p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Romero Perez L, Chen L, Lambertson D, Madura K. Sts1 can overcome the loss of Rad23 and Rpn10 and represents a novel regulator of the ubiquitin/proteasome pathway. J Biol Chem. 2007;282:35574-82 pubmed
    ..Despite these proteolytic defects, overall proteasome activity was increased in sts1-2. We propose that Sts1 is a new regulatory factor in the ubiquitin/proteasome pathway that controls the turnover of proteasome substrates...
  2. Elsasser S, Chandler Militello D, Müller B, Hanna J, Finley D. Rad23 and Rpn10 serve as alternative ubiquitin receptors for the proteasome. J Biol Chem. 2004;279:26817-22 pubmed
    ..recognizes ubiquitin chains through a specific subunit, Rpn10, and also recognizes chains indirectly through Rad23, a reversibly bound proteasome cofactor. Both binding events can be observed in purified biochemical systems...
  3. Biggins S, Ivanovska I, Rose M. Yeast ubiquitin-like genes are involved in duplication of the microtubule organizing center. J Cell Biol. 1996;133:1331-46 pubmed
    ..The only other known yeast ubiquitin-like protein is encoded by the nucleotide excision repair gene RAD23 (Watkins, J.F.,P. Sung, L. Prakash, and S. Prakash. 1993. Mol. Cell. Bio. 13:7757-7765)...
  4. Biswas S, Katiyar S, Li G, Zhou X, Lennarz W, Schindelin H. The N-terminus of yeast peptide: N-glycanase interacts with the DNA repair protein Rad23. Biochem Biophys Res Commun. 2004;323:149-55 pubmed
    ..involved in the proteasomal degradation of misfolded glycoproteins where it interacts with the DNA repair protein Rad23 as first detected in a yeast two-hybrid assay and subsequently confirmed by biochemical in vivo analyses...
  5. Schauber C, Chen L, Tongaonkar P, Vega I, Lambertson D, Potts W, et al. Rad23 links DNA repair to the ubiquitin/proteasome pathway. Nature. 1998;391:715-8 pubmed
    b>Rad23 is an evolutionarily conserved protein that is important for nucleotide excision repair...
  6. Ortolan T, Chen L, Tongaonkar P, Madura K. Rad23 stabilizes Rad4 from degradation by the Ub/proteasome pathway. Nucleic Acids Res. 2004;32:6490-500 pubmed
    b>Rad23 protein interacts with the nucleotide excision-repair (NER) factor Rad4, and the dimer can bind damaged DNA. Rad23 also binds ubiquitinated proteins and promotes their degradation by the proteasome...
  7. Heessen S, Masucci M, Dantuma N. The UBA2 domain functions as an intrinsic stabilization signal that protects Rad23 from proteasomal degradation. Mol Cell. 2005;18:225-35 pubmed
    The proteasome-interacting protein Rad23 is a long-lived protein. Interaction between Rad23 and the proteasome is required for Rad23's functions in nucleotide excision repair and ubiquitin-dependent degradation...
  8. Richly H, Rape M, Braun S, Rumpf S, Hoege C, Jentsch S. A series of ubiquitin binding factors connects CDC48/p97 to substrate multiubiquitylation and proteasomal targeting. Cell. 2005;120:73-84 pubmed
    ..In yeast, this escort pathway guides a transcription factor from its activation in the cytosol to its final degradation and also mediates proteolysis at the endoplasmic reticulum by the ERAD pathway. ..
  9. Kim I, Mi K, Rao H. Multiple interactions of rad23 suggest a mechanism for ubiquitylated substrate delivery important in proteolysis. Mol Biol Cell. 2004;15:3357-65 pubmed
    The mechanism underlying the delivery of ubiquitylated substrates to the proteasome is poorly understood. Rad23 is a putative adaptor molecule for this process because it interacts with ubiquitin chains through its ubiquitin-associated ..

More Information

Publications79

  1. Russell S, Reed S, Huang W, Friedberg E, Johnston S. The 19S regulatory complex of the proteasome functions independently of proteolysis in nucleotide excision repair. Mol Cell. 1999;3:687-95 pubmed
    ..The proteasome interacts with the ubiquitin-like N terminus of Rad23, a nucleotide excision repair (NER) protein, in Saccharomyces cerevisiae...
  2. Rao H, Sastry A. Recognition of specific ubiquitin conjugates is important for the proteolytic functions of the ubiquitin-associated domain proteins Dsk2 and Rad23. J Biol Chem. 2002;277:11691-5 pubmed
    ..We show that Saccharomyces cerevisiae cells lacking two of these UBA proteins, Dsk2 and Rad23, are deficient in protein degradation mediated by the UFD pathway and that the intact UBA motif of Dsk2 is ..
  3. Rosenzweig R, Osmulski P, Gaczynska M, Glickman M. The central unit within the 19S regulatory particle of the proteasome. Nat Struct Mol Biol. 2008;15:573-80 pubmed publisher
    ..Similar pairing of units is found in unfoldases and nuclear transporters, exposing common features of these protein nanomachines. ..
  4. Saeki Y, Sone T, Toh e A, Yokosawa H. Identification of ubiquitin-like protein-binding subunits of the 26S proteasome. Biochem Biophys Res Commun. 2002;296:813-9 pubmed
    Ubiquitin-like proteins Rad23 and Dsk2 have recently been shown to be capable of binding both polyubiquitin chains and the 26S proteasome...
  5. Fatimababy A, Lin Y, Usharani R, Radjacommare R, Wang H, Tsai H, et al. Cross-species divergence of the major recognition pathways of ubiquitylated substrates for ubiquitin/26S proteasome-mediated proteolysis. FEBS J. 2010;277:796-816 pubmed publisher
    ..In contrast, both the RPN10 and RPN13 homologs play major roles in humans. For indirect recognition, the RAD23 and DSK2 homologs (except for the human DSK2 homolog) are major receptors...
  6. Saeki Y, Saitoh A, Toh e A, Yokosawa H. Ubiquitin-like proteins and Rpn10 play cooperative roles in ubiquitin-dependent proteolysis. Biochem Biophys Res Commun. 2002;293:986-92 pubmed
    ..Here, we report that a mutant with a triple deletion of RAD23, DSK2, and RPN10 genes accumulates large amounts of polyubiquitinated proteins, as is the case with a mutant with ..
  7. Kim I, Ahn J, Liu C, Tanabe K, Apodaca J, Suzuki T, et al. The Png1-Rad23 complex regulates glycoprotein turnover. J Cell Biol. 2006;172:211-9 pubmed
    ..We demonstrate that the efficient degradation of glycosylated ricin A chain requires the Png1-Rad23 complex, suggesting that this complex couples protein deglycosylation and degradation...
  8. Rosenzweig R, Bronner V, Zhang D, Fushman D, Glickman M. Rpn1 and Rpn2 coordinate ubiquitin processing factors at proteasome. J Biol Chem. 2012;287:14659-71 pubmed publisher
    ..We found that two shuttles of this class, Rad23 and Dsk2, dock at two different receptor sites embedded within a single subunit of the 19 S proteasome regulatory ..
  9. Guzder S, Habraken Y, Sung P, Prakash L, Prakash S. Reconstitution of yeast nucleotide excision repair with purified Rad proteins, replication protein A, and transcription factor TFIIH. J Biol Chem. 1995;270:12973-6 pubmed
    ..The Rad14 protein, the Rad4-Rad23 complex, the Rad2 nuclease, the Rad1-Rad10 nuclease, replication protein A, and the RNA polymerase II ..
  10. Isasa M, Katz E, Kim W, Yugo V, González S, Kirkpatrick D, et al. Monoubiquitination of RPN10 regulates substrate recruitment to the proteasome. Mol Cell. 2010;38:733-45 pubmed publisher
    ..In addition, shuttling factors, such as Rad23, recruit substrates to the proteasome by delivering ubiquitinated proteins...
  11. Suzuki T, Park H, Kwofie M, Lennarz W. Rad23 provides a link between the Png1 deglycosylating enzyme and the 26 S proteasome in yeast. J Biol Chem. 2001;276:21601-7 pubmed
    In addition to a role in DNA repair events in yeast, several lines of evidence indicate that the Rad23 protein (Rad23p) may regulate the activity of the 26 S proteasome...
  12. Clarke D, Mondesert G, Segal M, Bertolaet B, Jensen S, Wolff M, et al. Dosage suppressors of pds1 implicate ubiquitin-associated domains in checkpoint control. Mol Cell Biol. 2001;21:1997-2007 pubmed
    ..Therefore, Rad23p and Ddi1p participate in a subset of Pds1p-dependent cell cycle controls...
  13. Zhang D, Chen T, Ziv I, Rosenzweig R, Matiuhin Y, Bronner V, et al. Together, Rpn10 and Dsk2 can serve as a polyubiquitin chain-length sensor. Mol Cell. 2009;36:1018-33 pubmed publisher
    ..We propose a mechanism for a malleable ubiquitin signal that depends both on chain length and combination of receptors to produce tetraubiquitin as an efficient signal threshold. ..
  14. Gong F, Fahy D, Smerdon M. Rad4-Rad23 interaction with SWI/SNF links ATP-dependent chromatin remodeling with nucleotide excision repair. Nat Struct Mol Biol. 2006;13:902-7 pubmed
    ..complex in Saccharomyces cerevisiae, copurify with the NER damage-recognition heterodimer Rad4-Rad23. This interaction between SWI/SNF and Rad4-Rad23 is stimulated by UV irradiation...
  15. Medicherla B, Kostova Z, Schaefer A, Wolf D. A genomic screen identifies Dsk2p and Rad23p as essential components of ER-associated degradation. EMBO Rep. 2004;5:692-7 pubmed
    ..Among the new gene products, we identified Dsk2p and Rad23p. We show that these two proteins are probably delivery factors for ubiquitinated ER substrates to the proteasome, ..
  16. Liu C, van Dyk D, Li Y, Andrews B, Rao H. A genome-wide synthetic dosage lethality screen reveals multiple pathways that require the functioning of ubiquitin-binding proteins Rad23 and Dsk2. BMC Biol. 2009;7:75 pubmed publisher
    ..Recent studies indicate that ubiquitin-binding proteins (e.g. Rad23, Dsk2, Rpn10) play a pivotal role in transferring ubiquitylated proteins to the proteasome...
  17. Dantuma N, Heinen C, Hoogstraten D. The ubiquitin receptor Rad23: at the crossroads of nucleotide excision repair and proteasomal degradation. DNA Repair (Amst). 2009;8:449-60 pubmed publisher
    ..between the ubiquitin/proteasome system (UPS) and DNA repair is the yeast nucleotide excision repair (NER) protein Rad23 and its human orthologs hHR23A and hHR23B...
  18. Ghaboosi N, Deshaies R. A conditional yeast E1 mutant blocks the ubiquitin-proteasome pathway and reveals a role for ubiquitin conjugates in targeting Rad23 to the proteasome. Mol Biol Cell. 2007;18:1953-63 pubmed
    ..to investigate the role ubiquitin conjugates play in the dynamic interaction of the UbL/UBA adaptor proteins Rad23 and Dsk2 with the proteasome...
  19. Lambertson D, Chen L, Madura K. Pleiotropic defects caused by loss of the proteasome-interacting factors Rad23 and Rpn10 of Saccharomyces cerevisiae. Genetics. 1999;153:69-79 pubmed
    b>Rad23 is a member of a novel class of proteins that contain unprocessed ubiquitin-like (UbL) domains. We showed recently that a small fraction of Rad23 can form an interaction with the 26S proteasome...
  20. Heinen C, Acs K, Hoogstraten D, Dantuma N. C-terminal UBA domains protect ubiquitin receptors by preventing initiation of protein degradation. Nat Commun. 2011;2:191 pubmed publisher
    The ubiquitin receptors Rad23 and Dsk2 deliver polyubiquitylated substrates to the proteasome for destruction...
  21. Bertolaet B, Clarke D, Wolff M, Watson M, Henze M, Divita G, et al. UBA domains of DNA damage-inducible proteins interact with ubiquitin. Nat Struct Biol. 2001;8:417-22 pubmed
    b>Rad23 is a highly conserved protein involved in nucleotide excision repair (NER) that associates with the proteasome via its N-terminus. Its C-terminal ubiquitin-associated (UBA) domain is evolutionarily conserved from yeast to humans...
  22. Ortolan T, Tongaonkar P, Lambertson D, Chen L, Schauber C, Madura K. The DNA repair protein rad23 is a negative regulator of multi-ubiquitin chain assembly. Nat Cell Biol. 2000;2:601-8 pubmed
    b>Rad23 is a nucleotide-excision repair protein with a previously unknown biochemical function. We determined that yeast and human Rad23 inhibited multi-ubiquitin (Ub) chain formation and the degradation of proteolytic substrates...
  23. Verma R, Oania R, Graumann J, Deshaies R. Multiubiquitin chain receptors define a layer of substrate selectivity in the ubiquitin-proteasome system. Cell. 2004;118:99-110 pubmed
    ..Genetic studies suggest a role for the multiubiquitin chain binding proteins (MCBPs) Rad23 and Rpn10 in recruitment, but biochemical studies implicate the Rpt5 ATPase...
  24. Li Y, Yan J, Kim I, Liu C, Huo K, Rao H. Rad4 regulates protein turnover at a postubiquitylation step. Mol Biol Cell. 2010;21:177-85 pubmed publisher
    The ubiquitin (Ub)-binding protein Rad23 plays an important role in facilitating the transfer of substrates to the proteasome. However, the mechanism underlying Rad23's function in proteolysis remains unknown...
  25. Kang Y, Vossler R, Diaz Martinez L, Winter N, Clarke D, Walters K. UBL/UBA ubiquitin receptor proteins bind a common tetraubiquitin chain. J Mol Biol. 2006;356:1027-35 pubmed
    ..is an intensely studied group that contains both ubiquitin-like (UBL) and ubiquitin-associated (UBA) domains: Rad23, Ddi1 and Dsk2...
  26. Lee J, Choi J, Lee C, Yi K, Cho Y. Structure of a peptide:N-glycanase-Rad23 complex: insight into the deglycosylation for denatured glycoproteins. Proc Natl Acad Sci U S A. 2005;102:9144-9 pubmed
    ..yPNGase is formed with three domains, a core domain containing a Cys-His-Asp triad, a Zn-binding domain, and a Rad23-binding domain...
  27. Hänzelmann P, Stingele J, Hofmann K, Schindelin H, Raasi S. The yeast E4 ubiquitin ligase Ufd2 interacts with the ubiquitin-like domains of Rad23 and Dsk2 via a novel and distinct ubiquitin-like binding domain. J Biol Chem. 2010;285:20390-8 pubmed publisher
    ..A common interaction of the budding yeast UBL-UBA proteins Rad23 and Dsk2 with the E4 ubiquitin ligase Ufd2 has been described in endoplasmic reticulum-associated degradation ..
  28. Chen L, Madura K. Centrin/Cdc31 is a novel regulator of protein degradation. Mol Cell Biol. 2008;28:1829-40 pubmed
    b>Rad23 is required for efficient protein degradation and performs an important role in nucleotide excision repair...
  29. Bertolaet B, Clarke D, Wolff M, Watson M, Henze M, Divita G, et al. UBA domains mediate protein-protein interactions between two DNA damage-inducible proteins. J Mol Biol. 2001;313:955-63 pubmed
    The Saccharomyces cerevisiae genes RAD23 and DDI1 were identified in a screen for multicopy suppressors of the temperature-sensitivity of a mutant allele of S. cerevisiae PDS1...
  30. Xie Z, Liu S, Zhang Y, Wang Z. Roles of Rad23 protein in yeast nucleotide excision repair. Nucleic Acids Res. 2004;32:5981-90 pubmed
    ..Most NER proteins are indispensable for repair. In contrast, the yeast Rad23 represents a class of accessory NER proteins, without which NER activity is reduced but not eliminated...
  31. Elsasser S, Gali R, Schwickart M, Larsen C, Leggett D, Müller B, et al. Proteasome subunit Rpn1 binds ubiquitin-like protein domains. Nat Cell Biol. 2002;4:725-30 pubmed
    The yeast protein Rad23 belongs to a diverse family of proteins that contain an amino-terminal ubiquitin-like (UBL) domain...
  32. Lambertson D, Chen L, Madura K. Investigating the importance of proteasome-interaction for Rad23 function. Curr Genet. 2003;42:199-208 pubmed
    b>Rad23 contributes to diverse cellular functions that include DNA repair, stress response and growth control...
  33. Chen L, Madura K. Rad23 promotes the targeting of proteolytic substrates to the proteasome. Mol Cell Biol. 2002;22:4902-13 pubmed
    b>Rad23 contains a ubiquitin-like domain (UbL(R23)) that interacts with catalytically active proteasomes and two ubiquitin (Ub)-associated (UBA) sequences that bind Ub...
  34. Matiuhin Y, Kirkpatrick D, Ziv I, Kim W, Dakshinamurthy A, Kleifeld O, et al. Extraproteasomal Rpn10 restricts access of the polyubiquitin-binding protein Dsk2 to proteasome. Mol Cell. 2008;32:415-25 pubmed publisher
    ..This work highlights the importance of polyubiquitin shuttles such as Rpn10 and Dsk2 in controlling the ubiquitin landscape. ..
  35. Liu C, van Dyk D, Choe V, Yan J, Majumder S, Costanzo M, et al. Ubiquitin ligase Ufd2 is required for efficient degradation of Mps1 kinase. J Biol Chem. 2011;286:43660-7 pubmed publisher
    ..We demonstrate that Ufd2-mediated Mps1 degradation is conserved in humans. Our results underscore the significance of Ufd2 in proteolysis and further suggest that Ufd2-like enzymes regulate far more substrates than previously envisioned...
  36. Mao P, Smerdon M. Yeast deubiquitinase Ubp3 interacts with the 26 S proteasome to facilitate Rad4 degradation. J Biol Chem. 2010;285:37542-50 pubmed publisher
    ..Yeast Rad4-Rad23 heterodimer is responsible for recognizing DNA damage in nucleotide excision repair (NER)...
  37. Shi Y, Chen X, Elsasser S, Stocks B, Tian G, Lee B, et al. Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome. Science. 2016;351: pubmed publisher
    ..Thus, a two-site recognition domain intrinsic to the proteasome uses distinct ubiquitin-fold ligands to assemble substrates, shuttling factors, and a deubiquitinating enzyme. ..
  38. Schiestl R, Prakash S. Interactions of the RAD7 and RAD23 excision repair genes of Saccharomyces cerevisiae with DNA repair genes in different epistasis groups. Curr Genet. 1989;16:219-23 pubmed
    The RAD7 and RAD23 genes of S. cerevisiae affect the efficiency of excision repair of UV-damaged DNA...
  39. Gödderz D, Giovannucci T, Lalakova J, Menendez Benito V, Dantuma N. The deubiquitylating enzyme Ubp12 regulates Rad23-dependent proteasomal degradation. J Cell Sci. 2017;130:3336-3346 pubmed publisher
    The consecutive actions of the ubiquitin-selective segregase Cdc48 and the ubiquitin shuttle factor Rad23 result in the delivery of ubiquitylated proteins at the proteasome...
  40. Rodriguez K, Talamantez J, Huang W, Reed S, Wang Z, Chen L, et al. Affinity purification and partial characterization of a yeast multiprotein complex for nucleotide excision repair using histidine-tagged Rad14 protein. J Biol Chem. 1998;273:34180-9 pubmed
    ..of this protein from yeast nuclear extracts resulted in the co-purification of Rad1, Rad7, Rad10, Rad16, Rad23, RPA, RPB1, and TFIIH proteins, whereas none of these proteins bound to the affinity resin in the absence of ..
  41. Sun L, Johnston S, Kodadek T. Physical association of the APIS complex and general transcription factors. Biochem Biophys Res Commun. 2002;296:991-9 pubmed
    ..These data add to the growing body of evidence that the APIS complex has a role in transcription, independent of its role in proteolysis and, furthermore, argues that it functions in association with the general transcription complex. ..
  42. Ishii T, Funakoshi M, Kobayashi H. Yeast Pth2 is a UBL domain-binding protein that participates in the ubiquitin-proteasome pathway. EMBO J. 2006;25:5492-503 pubmed
    Ubiquitin-like (UBL)-ubiquitin-associated (UBA) proteins such as Rad23 and Dsk2 mediate the delivery of polyubiquitinated proteins to the proteasome in the ubiquitin-proteasome pathway...
  43. Wei S, Friedberg E. A fragment of the yeast DNA repair protein Rad4 confers toxicity to E. coli and is required for its interaction with Rad7 protein. Mutat Res. 1998;400:127-33 pubmed
    ..coli when expressed alone. The deletion mutant retains its ability to interact with Rad23 protein but not with Rad7 protein and is defective in nucleotide excision repair...
  44. den Dulk B, Sun S, de Ruijter M, Brandsma J, Brouwer J. Rad33, a new factor involved in nucleotide excision repair in Saccharomyces cerevisiae. DNA Repair (Amst). 2006;5:683-92 pubmed
    In Saccharomyces cerevisiae the Rad4-Rad23 complex is involved in initial damage recognition and responsible for recruiting the other NER proteins to the site of the lesion...
  45. Verma R, Peters N, D Onofrio M, Tochtrop G, Sakamoto K, Varadan R, et al. Ubistatins inhibit proteasome-dependent degradation by binding the ubiquitin chain. Science. 2004;306:117-20 pubmed
    ..The same interface is recognized by ubiquitin-chain receptors of the proteasome, indicating that ubistatins act by disrupting a critical protein-protein interaction in the ubiquitin-proteasome system. ..
  46. Wang Z, Wei S, Reed S, Wu X, Svejstrup J, Feaver W, et al. The RAD7, RAD16, and RAD23 genes of Saccharomyces cerevisiae: requirement for transcription-independent nucleotide excision repair in vitro and interactions between the gene products. Mol Cell Biol. 1997;17:635-43 pubmed
    ..In the yeast Saccharomyces cerevisiae, the RAD7, RAD16, and RAD23 genes have been specifically implicated in NER of certain transcriptionally repressed loci and in the ..
  47. Prakash S, Prakash L. Nucleotide excision repair in yeast. Mutat Res. 2000;451:13-24 pubmed
    ..This reaction requires the damage binding factors Rad14, RPA, and the Rad4-Rad23 complex, the transcription factor TFIIH which contains the two DNA helicases Rad3 and Rad25, essential for ..
  48. Chuang S, Chen L, Lambertson D, Anand M, Kinzy T, Madura K. Proteasome-mediated degradation of cotranslationally damaged proteins involves translation elongation factor 1A. Mol Cell Biol. 2005;25:403-13 pubmed
    b>Rad23 and Rpn10 play synergistic roles in the recognition of ubiquitinated proteins by the proteasome, and loss of both proteins causes growth and proteolytic defects...
  49. Kong M, Liu L, Chen X, Driscoll K, Mao P, Böhm S, et al. Single-Molecule Imaging Reveals that Rad4 Employs a Dynamic DNA Damage Recognition Process. Mol Cell. 2016;64:376-387 pubmed publisher
    ..Quantum dot-labeled Rad4-Rad23 (yeast XPC-RAD23B ortholog) forms non-motile complexes or conducts a one-dimensional search via either random ..
  50. Saeki Y, Kudo T, Sone T, Kikuchi Y, Yokosawa H, Toh e A, et al. Lysine 63-linked polyubiquitin chain may serve as a targeting signal for the 26S proteasome. EMBO J. 2009;28:359-71 pubmed publisher
    ..These results raise the possibility that Lys63-linked ubiquitin chain also serves as a targeting signal for the 26S proteaseome in vivo. ..
  51. Liang R, Chen L, Ko B, Shen Y, Li Y, Chen B, et al. Rad23 interaction with the proteasome is regulated by phosphorylation of its ubiquitin-like (UbL) domain. J Mol Biol. 2014;426:4049-60 pubmed publisher
    b>Rad23 was identified as a DNA repair protein, although a role in protein degradation has been described...
  52. Chandra A, Chen L, Madura K. Synthetic lethality of rpn11-1 rpn10? is linked to altered proteasome assembly and activity. Curr Genet. 2010;56:543-57 pubmed publisher
    ..Based on these findings, we propose that the lethality of rpn11-1 rpn10? results primarily from altered proteasome integrity. It is conceivable that Rpn10/Rpn11 interaction couples proteasome assembly to substrate binding. ..
  53. Rumpf S, Jentsch S. Functional division of substrate processing cofactors of the ubiquitin-selective Cdc48 chaperone. Mol Cell. 2006;21:261-9 pubmed
    ..We propose that the balance between the distinct substrate-processing cofactors may determine whether a substrate is multiubiquitylated and routed to the proteasome for degradation or deubiquitylated and/or released for other purposes. ..
  54. Ishii T, Funakoshi M, Kobayashi H, Sekiguchi T. Yeast Irc22 Is a Novel Dsk2-Interacting Protein that Is Involved in Salt Tolerance. Cells. 2014;3:180-98 pubmed publisher
    ..Irc22 interacted with Dsk2 in yeast cells, but not with Rad23 and Ddi1. Ubiquitin-dependent degradation was impaired mildly by over-expression or disruption of IRC22...
  55. Hosomi A, Tanabe K, Hirayama H, Kim I, Rao H, Suzuki T. Identification of an Htm1 (EDEM)-dependent, Mns1-independent Endoplasmic Reticulum-associated Degradation (ERAD) pathway in Saccharomyces cerevisiae: application of a novel assay for glycoprotein ERAD. J Biol Chem. 2010;285:24324-34 pubmed publisher
    ..The newly established RTL assay will allow us to gain further insight into the mechanisms involved in the Png1-dependent ERAD-L pathway. ..
  56. Rosenfeld L, Culotta V. Phosphate disruption and metal toxicity in Saccharomyces cerevisiae: effects of RAD23 and the histone chaperone HPC2. Biochem Biophys Res Commun. 2012;418:414-9 pubmed publisher
    ..Our studies are consistent with a model in which Rad23p partners with the deglycosylating enzyme Png1p to reduce manganese toxicity through proteosomal degradation of ..
  57. Tempé D, Brengues M, Mayonove P, Bensaad H, Lacrouts C, Morris M. The alpha helix of ubiquitin interacts with yeast cyclin-dependent kinase subunit CKS1. Biochemistry. 2007;46:45-54 pubmed
  58. Silver H, Nissley J, Reed S, Hou Y, Johnson E. A role for SUMO in nucleotide excision repair. DNA Repair (Amst). 2011;10:1243-51 pubmed publisher
    ..Collectively, these results suggest that SIZ-dependent sumoylation may modulate the activity of multiple proteins to promote efficient NER. ..
  59. Ramsey K, Smith J, Dasgupta A, Maqani N, Grant P, Auble D. The NEF4 complex regulates Rad4 levels and utilizes Snf2/Swi2-related ATPase activity for nucleotide excision repair. Mol Cell Biol. 2004;24:6362-78 pubmed
    ..ATPase is important for NEF4 function in vivo, and genetic analysis uncovered new interactions between NEF4 and Rad23, a repair factor that links repair to proteasome function...
  60. Chen X, Velmurugu Y, Zheng G, Park B, Shim Y, Kim Y, et al. Kinetic gating mechanism of DNA damage recognition by Rad4/XPC. Nat Commun. 2015;6:5849 pubmed publisher
    ..Kinetic gating may be a general mechanism used by site-specific DNA-binding proteins to minimize time-consuming interrogations of non-target sites. ..
  61. Podlaska A, McIntyre J, Skoneczna A, Sledziewska Gojska E. The link between 20S proteasome activity and post-replication DNA repair in Saccharomyces cerevisiae. Mol Microbiol. 2003;49:1321-32 pubmed
    ..We show that UMP1 is not epistatic to RAD23 and RAD2, which are involved in the nucleotide excision repair (NER) pathway...
  62. Wade S, Poorey K, Bekiranov S, Auble D. The Snf1 kinase and proteasome-associated Rad23 regulate UV-responsive gene expression. EMBO J. 2009;28:2919-31 pubmed publisher
    ..Here we identify two new regulators of the UV response transcriptome: Snf1, a nutrient-sensing kinase, and Rad23, a nucleotide excision repair factor with no previously known function in transcriptional control...
  63. Liu C, Liu W, Ye Y, Li W. Ufd2p synthesizes branched ubiquitin chains to promote the degradation of substrates modified with atypical chains. Nat Commun. 2017;8:14274 pubmed publisher
    ..Thus, our results suggest that Ufd2p functions by switching ubiquitin chain linkages to allow the degradation of proteins modified with a ubiquitin linkage, which is normally not targeted to the proteasome. ..
  64. Hobeika M, Brockmann C, Iglesias N, Gwizdek C, Neuhaus D, Stutz F, et al. Coordination of Hpr1 and ubiquitin binding by the UBA domain of the mRNA export factor Mex67. Mol Biol Cell. 2007;18:2561-8 pubmed
  65. Guzder S, Bailly V, Sung P, Prakash L, Prakash S. Yeast DNA repair protein RAD23 promotes complex formation between transcription factor TFIIH and DNA damage recognition factor RAD14. J Biol Chem. 1995;270:8385-8 pubmed
    ..Here, we demonstrate a higher order complex consisting of TFIIH, RAD14, and another NER protein RAD23, and complex formation between TFIIH and RAD14 is facilitated by the RAD23 protein.
  66. Apodaca J, Ahn J, Kim I, Rao H. Analysis of ubiquitin chain-binding proteins by two-hybrid methods. Methods Enzymol. 2005;399:157-64 pubmed
    ..Yeast two-hybrid assay can be a powerful approach to dissect the interaction between Ub and its binding proteins and also the function of these Ub-chain binding proteins in vivo. ..
  67. Zhang L, Jones K, Smerdon M, Gong F. Assays for chromatin remodeling during nucleotide excision repair in Saccharomyces cerevisiae. Methods. 2009;48:19-22 pubmed publisher
    ..We have used the methods described here to demonstrate that the SWI/SNF chromatin remodeling complex is involved in chromatin rearrangement during NER. ..
  68. Diaz Martinez L, Kang Y, Walters K, Clarke D. Yeast UBL-UBA proteins have partially redundant functions in cell cycle control. Cell Div. 2006;1:28 pubmed
    ..There are three UBL-UBA containing proteins in budding yeast: Ddi1, Dsk2 and Rad23, which have been demonstrated to play regulatory roles in targeting ubiquitinated substrates to the proteasome for ..
  69. Zhou Z, Humphryes N, van Eijk P, Waters R, Yu S, Kraehenbuehl R, et al. UV induced ubiquitination of the yeast Rad4-Rad23 complex promotes survival by regulating cellular dNTP pools. Nucleic Acids Res. 2015;43:7360-70 pubmed publisher
    ..profiling and chromatin immunoprecipitation, we demonstrate that in the absence of DNA damage the yeast Rad4-Rad23 nucleotide excision repair complex binds to the promoters of certain DNA damage response genes including DUN1, ..
  70. Chen X, Randles L, Shi K, Tarasov S, Aihara H, Walters K. Structures of Rpn1 T1:Rad23 and hRpn13:hPLIC2 Reveal Distinct Binding Mechanisms between Substrate Receptors and Shuttle Factors of the Proteasome. Structure. 2016;24:1257-1270 pubmed publisher
    ..in the proteasome bind substrates by interacting with conjugated ubiquitin chains and/or shuttle factors (Rad23/HR23, Dsk2/PLIC/ubiquilin, Ddi1) that carry ubiquitinated substrates to proteasomes...