Genomes and Genes
Gene Symbol: RAD17
Species: Saccharomyces cerevisiae S288c
- Grushcow J, Holzen T, Park K, Weinert T, Lichten M, Bishop D. Saccharomyces cerevisiae checkpoint genes MEC1, RAD17 and RAD24 are required for normal meiotic recombination partner choice. Genetics. 1999;153:607-20 pubmed..Bypass of dmc1 arrest by mutation of the DNA damage checkpoint genes MEC1, RAD17, or RAD24 results in a dramatic loss of spore viability, suggesting that these genes play an important role in ..
- Branzei D, Seki M, Onoda F, Enomoto T. The product of Saccharomyces cerevisiae WHIP/MGS1, a gene related to replication factor C genes, interacts functionally with DNA polymerase delta. Mol Genet Genomics. 2002;268:371-86 pubmed..Possible roles of Mgs1, DNA polymerase delta, Rad18 and Mms2 in replication and replication fork restart are discussed. ..
- Krejci L, Macris M, Li Y, Van Komen S, Villemain J, Ellenberger T, et al. Role of ATP hydrolysis in the antirecombinase function of Saccharomyces cerevisiae Srs2 protein. J Biol Chem. 2004;279:23193-9 pubmed..These results highlight the importance of the ATP hydrolysisfueled DNA motor activity in SRS2 functions. ..
- Majka J, Burgers P. Function of Rad17/Mec3/Ddc1 and its partial complexes in the DNA damage checkpoint. DNA Repair (Amst). 2005;4:1189-94 pubmedThe Saccharomyces cerevisiae heterotrimeric checkpoint clamp consisting of the Rad17, Mec3, and Ddc1 subunits (Rad17/3/1, the 9-1-1 complex in humans) is an early response factor to DNA damage in a signal transduction pathway leading to ..
- Zou L, Liu D, Elledge S. Replication protein A-mediated recruitment and activation of Rad17 complexes. Proc Natl Acad Sci U S A. 2003;100:13827-32 pubmedThe human Rad17-Rfc2-5 and Rad9-Rad1-Hus1 complexes play crucial roles in the activation of the ATR-mediated DNA damage and DNA replication stress response pathways...
- Vinton P, Weinert T. A Slowed Cell Cycle Stabilizes the Budding Yeast Genome. Genetics. 2017;206:811-828 pubmed publisher..And, some chromosome errors may not signal checkpoint-mediated responses, or do not sufficiently signal to allow correction, and their correction benefits from this "time checkpoint." ..
- Koltovaia N, Nikulushkina I, Poshchina M, Devin A. [Interaction between checkpoint genes RAD9, RAD17, RAD24, and RAD53 involved in the determination of yeast Saccharomyces cerevisiae sensitivity to ionizing radiation]. Genetika. 2008;44:761-70 pubmed..To clarify the role of checkpoint genes RAD9, RAD17, RAD24, and RAD53 in cell radioresistance, double mutants were analyzed for cell sensitivity to ionizing radiation...
- Vance J, Wilson T. Yeast Tdp1 and Rad1-Rad10 function as redundant pathways for repairing Top1 replicative damage. Proc Natl Acad Sci U S A. 2002;99:13669-74 pubmed..Finally, we show that yeast lacking the Rad1-Rad10-related proteins Mus81-Mms4 display a unique pattern of camptothecin sensitivity and suggest a concerted model for the action of these endonucleases. ..
- O Neill B, Szyjka S, Lis E, Bailey A, Yates J, Aparicio O, et al. Pph3-Psy2 is a phosphatase complex required for Rad53 dephosphorylation and replication fork restart during recovery from DNA damage. Proc Natl Acad Sci U S A. 2007;104:9290-5 pubmed..These findings suggest that Rad53 regulates replication fork restart and initiation of late firing origins independently and that regulation of these processes is mediated by specific Rad53 phosphatases. ..
- Gómez González B, Felipe Abrio I, Aguilera A. The S-phase checkpoint is required to respond to R-loops accumulated in THO mutants. Mol Cell Biol. 2009;29:5203-13 pubmed publisher..In light of these results, we propose a model in which R-loop-mediated recombination is explained by template switching. ..
- Gonzalez Huici V, Szakal B, Urulangodi M, Psakhye I, Castellucci F, Menolfi D, et al. DNA bending facilitates the error-free DNA damage tolerance pathway and upholds genome integrity. EMBO J. 2014;33:327-40 pubmed publisher..Together, the results suggest that replication-associated topological changes involving the molecular DNA bender, Hmo1, set the stage for dedicated repair reactions that limit errors during replication and impact on genome stability. ..
- Lindgren A, Bungard D, Pierce M, Xie J, Vershon A, Winter E. The pachytene checkpoint in Saccharomyces cerevisiae requires the Sum1 transcriptional repressor. EMBO J. 2000;19:6489-97 pubmedSaccharomyces cerevisiae mutants that fail to complete meiotic recombination are blocked by the RAD17/RAD24/MEC1 checkpoint signaling pathway in pachytene when early sporulation genes are expressed...
- Klauer A, van Hoof A. Genetic interactions suggest multiple distinct roles of the arch and core helicase domains of Mtr4 in Rrp6 and exosome function. Nucleic Acids Res. 2013;41:533-41 pubmed publisher..Finally, we show that the arch domain of Ski2, the cytoplasmic counterpart of Mtr4, is required for Ski2's function, thereby confirming that the arch domains of these cofactors function independently of Rrp6. ..
- Sabbioneda S, Minesinger B, Giannattasio M, Plevani P, Muzi Falconi M, Jinks Robertson S. The 9-1-1 checkpoint clamp physically interacts with polzeta and is partially required for spontaneous polzeta-dependent mutagenesis in Saccharomyces cerevisiae. J Biol Chem. 2005;280:38657-65 pubmed..The Saccharomyces cerevisiae checkpoint proteins Ddc1, Rad17, and Mec3 form a clamp-like structure (the 9-1-1 clamp) that has physical similarity to the homotrimeric sliding ..
- Yasutis K, Vignali M, Ryder M, Tameire F, Dighe S, Fields S, et al. Zds2p regulates Swe1p-dependent polarized cell growth in Saccharomyces cerevisiae via a novel Cdc55p interaction domain. Mol Biol Cell. 2010;21:4373-86 pubmed publisher..This negative regulation requires the CDC55 gene. Together these data indicate that the Cdc55p/Zds2p module has a function in the regulation of the Swe1p-dependent G2/M checkpoint. ..
- Reha Krantz L, Siddique M, Murphy K, Tam A, O Carroll M, Lou S, et al. Drug-sensitive DNA polymerase ? reveals a role for mismatch repair in checkpoint activation in yeast. Genetics. 2011;189:1211-24 pubmed publisher..arrest requires all of the components of the S-phase DNA damage checkpoint: Mec1, Rad9, the DNA damage clamp Ddc1-Rad17-Mec3, and the Rad24-dependent clamp loader, but does not depend on Mrc1, which acts as the signaling adapter for ..
- Corda Y, Schramke V, Longhese M, Smokvina T, Paciotti V, Brevet V, et al. Interaction between Set1p and checkpoint protein Mec3p in DNA repair and telomere functions. Nat Genet. 1999;21:204-8 pubmed..Our findings indicate that interactions between SET1 and MEC3 have a role in DNA repair and telomere function. ..
- Zhang H, Zhu Z, Vidanes G, Mbangkollo D, Liu Y, Siede W. Characterization of DNA damage-stimulated self-interaction of Saccharomyces cerevisiae checkpoint protein Rad17p. J Biol Chem. 2001;276:26715-23 pubmedSaccharomyces cerevisiae Rad17p is necessary for cell cycle checkpoint arrests in response to DNA damage...
- Fu Y, Zhu Y, Zhang K, Yeung M, Durocher D, Xiao W. Rad6-Rad18 mediates a eukaryotic SOS response by ubiquitinating the 9-1-1 checkpoint clamp. Cell. 2008;133:601-11 pubmed publisher..induction as well as checkpoint functions by catalyzing monoubiquitination at the K197 residue of the Rad17 subunit of the 9-1-1 complex...
- Karumbati A, Wilson T. Abrogation of the Chk1-Pds1 checkpoint leads to tolerance of persistent single-strand breaks in Saccharomyces cerevisiae. Genetics. 2005;169:1833-44 pubmed..We propose a model in which recombinational repair during S phase coupled with failure of the metaphase-anaphase checkpoint allows for tolerance of persistent single-strand breaks at the expense of genome stability. ..
- Shinohara M, Hayashihara K, Grubb J, Bishop D, Shinohara A. DNA damage response clamp 9-1-1 promotes assembly of ZMM proteins for formation of crossovers and synaptonemal complex. J Cell Sci. 2015;128:1494-506 pubmed publisher..Here, we examined, in budding yeast, the meiotic role of the heterotrimeric DNA damage response clamp composed of Rad17, Ddc1 and Mec3 (known as '9-1-1' in other organisms) and a component of the clamp loader, Rad24 (known as Rad17 in ..
- Majka J, Chung B, Burgers P. Requirement for ATP by the DNA damage checkpoint clamp loader. J Biol Chem. 2004;279:20921-6 pubmed..This complex loads the heterotrimeric DNA damage clamp consisting of Rad17, Mec3, and Ddc1 (Rad17/3/1) onto partial duplex DNA in an ATP-dependent manner...
- Hepworth S, Friesen H, Segall J. NDT80 and the meiotic recombination checkpoint regulate expression of middle sporulation-specific genes in Saccharomyces cerevisiae. Mol Cell Biol. 1998;18:5750-61 pubmed..in a dmc1 strain, which fails to enter the meiotic divisions because a defect in meiotic recombination leads to a RAD17-dependent checkpoint arrest...
- Sawarynski K, Najor N, Kepsel A, Brush G. Sic1-induced DNA rereplication during meiosis. Proc Natl Acad Sci U S A. 2009;106:232-7 pubmed publisher..Deletion of DMC1 abolished DNA rereplication, but additional deletion of RAD17 restored the original phenotype...
- Davies A, Neiss A, Ulrich H. Ubiquitylation of the 9-1-1 checkpoint clamp is independent of rad6-rad18 and DNA damage. Cell. 2010;141:1080-7 pubmed publisher..The relevant ubiquitylation target was identified as budding yeast Rad17, a subunit of the PCNA-like 9-1-1 checkpoint clamp...
- Kile A, Koepp D. Activation of the S-phase checkpoint inhibits degradation of the F-box protein Dia2. Mol Cell Biol. 2010;30:160-71 pubmed publisher..Together, our results suggest that Dia2 protein turnover does not involve an autocatalytic mechanism and that Dia2 proteolysis is inhibited by activation of the replication stress response. ..
- Garrey S, Katolik A, Prekeris M, Li X, York K, Bernards S, et al. A homolog of lariat-debranching enzyme modulates turnover of branched RNA. RNA. 2014;20:1337-48 pubmed publisher..Drn1 enhances Dbr1-mediated turnover of lariat-intermediates and lariat-intron products, indicating that branched RNA turnover is regulated at multiple steps during splicing...
- Burgess R, Han J, Zhang Z. The Ddc1-Mec3-Rad17 sliding clamp regulates histone-histone chaperone interactions and DNA replication-coupled nucleosome assembly in budding yeast. J Biol Chem. 2014;289:10518-29 pubmed publisher..Here we describe synthetic genetic interactions between RTT106 and the DDC1-MEC3-RAD17 (9-1-1) complex, a sliding clamp functioning in the S phase DNA damage and replication checkpoint response, upon ..
- Fiorani P, Reid R, Schepis A, Jacquiau H, Guo H, Thimmaiah P, et al. The deubiquitinating enzyme Doa4p protects cells from DNA topoisomerase I poisons. J Biol Chem. 2004;279:21271-81 pubmed..Additional studies suggest a role for Doa4p in the Rad9p checkpoint response to Top1p poisons. These findings indicate a functional link between ubiquitin-mediated proteolysis and cellular resistance to CPT-induced DNA damage. ..
- Giannattasio M, Sabbioneda S, Minuzzo M, Plevani P, Muzi Falconi M. Correlation between checkpoint activation and in vivo assembly of the yeast checkpoint complex Rad17-Mec3-Ddc1. J Biol Chem. 2003;278:22303-8 pubmedb>Rad17-Mec3-Ddc1 forms a proliferating cell nuclear antigen-like complex that is required for the DNA damage response in Saccharomyces cerevisiae and acts at an early step of the signal transduction cascade activated by DNA lesions...
- Pak J, Segall J. Role of Ndt80, Sum1, and Swe1 as targets of the meiotic recombination checkpoint that control exit from pachytene and spore formation in Saccharomyces cerevisiae. Mol Cell Biol. 2002;22:6430-40 pubmed..Spore morphogenesis, however, requires efficient and timely activation of MSGs, which we speculate was achieved in dmc1/dmc1 sum1/sum1 cells by premature expression of NDT80. ..
- Barbour L, Ball L, Zhang K, Xiao W. DNA damage checkpoints are involved in postreplication repair. Genetics. 2006;174:1789-800 pubmed..These results suggest that a damage checkpoint is essential for tolerance mediated by both the error-free and error-prone branches of PRR. ..
- Koepp D, Kile A, Swaminathan S, Rodriguez Rivera V. The F-box protein Dia2 regulates DNA replication. Mol Biol Cell. 2006;17:1540-8 pubmed..Interestingly, prolonging G1-phase in dia2 cells prevents the accumulation of DNA damage in S-phase. We propose that Dia2 is an origin-binding protein that plays a role in regulating DNA replication. ..
- Cardone J, Revers L, Machado R, Bonatto D, Brendel M, Henriques J. Psoralen-sensitive mutant pso9-1 of Saccharomyces cerevisiae contains a mutant allele of the DNA damage checkpoint gene MEC3. DNA Repair (Amst). 2006;5:163-71 pubmed..This mutation affected the binding properties of Pso9-1p, abolishing its interactions with both Rad17p and Ddc1p...
- Pike B, Yongkiettrakul S, Tsai M, Heierhorst J. Mdt1, a novel Rad53 FHA1 domain-interacting protein, modulates DNA damage tolerance and G(2)/M cell cycle progression in Saccharomyces cerevisiae. Mol Cell Biol. 2004;24:2779-88 pubmed..The data indicate that Mdt1 is involved in normal G(2)/M cell cycle progression and is a novel target of checkpoint-dependent cell cycle arrest pathways. ..
- BÃ¶hm S, Szakal B, Herken B, Sullivan M, Mihalevic M, Kabbinavar F, et al. The Budding Yeast Ubiquitin Protease Ubp7 Is a Novel Component Involved in S Phase Progression. J Biol Chem. 2016;291:4442-52 pubmed publisher..In summary, our results suggest that Ubp7 contributes to S phase progression by affecting the chromatin state at replication forks, and we propose histone H2B ubiquitination as a potential substrate of Ubp7. ..
- Che J, Smith S, Kim Y, Shim E, Myung K, Lee S. Hyper-Acetylation of Histone H3K56 Limits Break-Induced Replication by Inhibiting Extensive Repair Synthesis. PLoS Genet. 2015;11:e1004990 pubmed publisher..and elevated spontaneous mutagenesis, the BIR defect in hst3Î” hst4Î” cannot be offset by the deletion of RAD17 or MMS22, but rather by the loss of RTT109 or ASF1, or in combination with the H3K56R mutation, which also ..
- de La Torre Ruiz M, Green C, Lowndes N. RAD9 and RAD24 define two additive, interacting branches of the DNA damage checkpoint pathway in budding yeast normally required for Rad53 modification and activation. EMBO J. 1998;17:2687-98 pubmedIn budding yeast, RAD9 and RAD24/RAD17/MEC3 are believed to function upstream of MEC1 and RAD53 in signalling the presence of DNA damage...
- Ngo G, Balakrishnan L, Dubarry M, Campbell J, Lydall D. The 9-1-1 checkpoint clamp stimulates DNA resection by Dna2-Sgs1 and Exo1. Nucleic Acids Res. 2014;42:10516-28 pubmed publisher..Our results provide important mechanistic insights into how DNA resection is regulated by checkpoint proteins and have implications for genome stability in eukaryotes. ..
- Collura A, Kemp P, Boiteux S. Abasic sites linked to dUTP incorporation in DNA are a major cause of spontaneous mutations in absence of base excision repair and Rad17-Mec3-Ddc1 (9-1-1) DNA damage checkpoint clamp in Saccharomyces cerevisiae. DNA Repair (Amst). 2012;11:294-303 pubmed publisher..The results show that mec1 sml1, rad53 sml1 and rad9 is synthetic lethal with apn1 apn2. In contrast, apn1 apn2 rad17, apn1 apn2 ddc1 and apn1 apn2 rad24 triple mutants are viable, although they exhibit a strong Can(R) spontaneous ..
- Wang H, Elledge S. Genetic and physical interactions between DPB11 and DDC1 in the yeast DNA damage response pathway. Genetics. 2002;160:1295-304 pubmed..Ddc1 is a DNA damage checkpoint protein, which, together with Mec3 and Rad17, has been proposed to form a PCNA-like complex and acts upstream in the DNA damage checkpoint pathways...
- Paulovich A, Armour C, Hartwell L. The Saccharomyces cerevisiae RAD9, RAD17, RAD24 and MEC3 genes are required for tolerating irreparable, ultraviolet-induced DNA damage. Genetics. 1998;150:75-93 pubmed..We find that RAD9, RAD17, RAD24, and MEC3 are required for UV-induced (although not spontaneous) mutagenesis, and that RAD9 and RAD17 (but ..
- Paulovich A, Margulies R, Garvik B, Hartwell L. RAD9, RAD17, and RAD24 are required for S phase regulation in Saccharomyces cerevisiae in response to DNA damage. Genetics. 1997;145:45-62 pubmed..In this report, we show that other genes (RAD9, RAD17, RAD24) involved in the DNA damage checkpoint pathway also play a role in regulating S phase in response to DNA ..
- Guénolé A, Srivas R, Vreeken K, Wang Z, Wang S, Krogan N, et al. Dissection of DNA damage responses using multiconditional genetic interaction maps. Mol Cell. 2013;49:346-58 pubmed publisher..Our multiconditional genetic interaction map provides a unique resource that identifies agent-specific and general DNA damage response pathways. ..
- Kapoor P, Bao Y, Xiao J, Luo J, Shen J, Persinger J, et al. Regulation of Mec1 kinase activity by the SWI/SNF chromatin remodeling complex. Genes Dev. 2015;29:591-602 pubmed publisher..Together, these findings identify a novel regulator of Mec1 kinase activity and suggest that ATP-dependent chromatin remodeling complexes can regulate nonchromatin substrates such as a checkpoint kinase. ..
- Miyajima A, Seki M, Onoda F, Shiratori M, Odagiri N, Ohta K, et al. Sgs1 helicase activity is required for mitotic but apparently not for meiotic functions. Mol Cell Biol. 2000;20:6399-409 pubmed..Disruption of the RED1 or RAD17 gene partially alleviated the poor-sporulation phenotype of sgs1 disruptants, indicating that portions of the ..
- Argunhan B, Farmer S, Leung W, Terentyev Y, Humphryes N, Tsubouchi T, et al. Direct and indirect control of the initiation of meiotic recombination by DNA damage checkpoint mechanisms in budding yeast. PLoS ONE. 2013;8:e65875 pubmed publisher..On the other hand, the absence of Rad17 (a critical component of the ATR pathway) lead to an increase in DSB formation (chromosomes VII and II were tested)..
- Iacovella M, Daly C, Kelly J, Michielsen A, Clyne R. Analysis of Polo-like kinase Cdc5 in the meiosis recombination checkpoint. Cell Cycle. 2010;9:1182-93 pubmed..The cdc5-ad mutation that renders cells unable to adapt to DNA damage in mitosis did not affect checkpoint adaptation in meiosis, indicating that the mechanisms of checkpoint adaptation in mitosis and meiosis are not fully conserved. ..
- Koltovaia N, Nikulushkina I, Kadyshevskaia E, Roshchina M, Devin A. [Interaction between checkpoint genes RAD9, RAD17, RAD24, and RAD53 involved in the determination of yeast Saccharomyces cerevisiae sensitivity to ionizing radiation]. Genetika. 2008;44:1045-55 pubmed..To clarify the role of checkpoint genes RAD9, RAD17, RAD24, and RAD53 in cell radioresistance, double mutants were analyzed for cell sensitivity to ionizing radiation...
- Odagiri N, Seki M, Onoda F, Yoshimura A, Watanabe S, Enomoto T. Budding yeast mms4 is epistatic with rad52 and the function of Mms4 can be replaced by a bacterial Holliday junction resolvase. DNA Repair (Amst). 2003;2:347-58 pubmed
- Shen J, Srivas R, Gross A, Li J, Jaehnig E, Sun S, et al. Chemogenetic profiling identifies RAD17 as synthetically lethal with checkpoint kinase inhibition. Oncotarget. 2015;6:35755-69 pubmed publisher..We identified eight interactions, including the Replication Factor C (RFC)-related protein RAD17. Clonogenic assays in RAD17 knockdown cell lines identified a substantial shift in sensitivity to checkpoint ..
- Bracesco N, Candreva E, Keszenman D, Sánchez A, Soria S, Dell M, et al. Roles of Saccharomyces cerevisiae RAD17 and CHK1 checkpoint genes in the repair of double-strand breaks in cycling cells. Radiat Environ Biophys. 2007;46:401-7 pubmed..We analysed the putative dual functions of Rad17 and Chk1 as checkpoints and in DNA repair using mutant strains of Saccharomyces cerevisiae...
- Myung K, Kolodner R. Suppression of genome instability by redundant S-phase checkpoint pathways in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2002;99:4500-7 pubmed..These data support the view that spontaneous genome rearrangements result from DNA replication errors and indicate that there is a high degree of redundancy among the checkpoints that act in S phase to suppress such genome instability. ..