Gene Symbol: RAD14
Description: Rad14p
Alias: Rad14p
Species: Saccharomyces cerevisiae S288c
Products:     RAD14

Top Publications

  1. Pages V, Santa Maria S, Prakash L, Prakash S. Role of DNA damage-induced replication checkpoint in promoting lesion bypass by translesion synthesis in yeast. Genes Dev. 2009;23:1438-49 pubmed publisher
  2. Prakash S, Prakash L. Nucleotide excision repair in yeast. Mutat Res. 2000;451:13-24 pubmed
    ..This reaction requires the damage binding factors Rad14, RPA, and the Rad4-Rad23 complex, the transcription factor TFIIH which contains the two DNA helicases Rad3 and ..
  3. Mardiros A, Benoun J, Haughton R, Baxter K, Kelson E, Fischhaber P. Rad10-YFP focus induction in response to UV depends on RAD14 in yeast. Acta Histochem. 2011;113:409-15 pubmed publisher
    b>Rad14 is a DNA damage recognition protein in yeast Nucleotide Excision Repair (NER) and believed to function early in the cascade of events...
  4. Guzder S, Sommers C, Prakash L, Prakash S. Complex formation with damage recognition protein Rad14 is essential for Saccharomyces cerevisiae Rad1-Rad10 nuclease to perform its function in nucleotide excision repair in vivo. Mol Cell Biol. 2006;26:1135-41 pubmed
    ..subassemblies: the Rad1-Rad10 nuclease, for example, forms a tight complex with the damage recognition protein Rad14, and the complex of Rad1-Rad10-Rad14 can be purified intact from yeast cells...
  5. Xie Z, Braithwaite E, Guo D, Zhao B, Geacintov N, Wang Z. Mutagenesis of benzo[a]pyrene diol epoxide in yeast: requirement for DNA polymerase zeta and involvement of DNA polymerase eta. Biochemistry. 2003;42:11253-62 pubmed
  6. Torres Ramos C, Johnson R, Prakash L, Prakash S. Evidence for the involvement of nucleotide excision repair in the removal of abasic sites in yeast. Mol Cell Biol. 2000;20:3522-8 pubmed
    ..A deficiency in the repair of AP sites may contribute to the internal cancers and progressive neurodegeneration that occur in XP patients. ..
  7. Neecke H, Lucchini G, Longhese M. Cell cycle progression in the presence of irreparable DNA damage is controlled by a Mec1- and Rad53-dependent checkpoint in budding yeast. EMBO J. 1999;18:4485-97 pubmed
  8. Guzder S, Sung P, Prakash L, Prakash S. Yeast DNA-repair gene RAD14 encodes a zinc metalloprotein with affinity for ultraviolet-damaged DNA. Proc Natl Acad Sci U S A. 1993;90:5433-7 pubmed
    ..The Saccharomyces cerevisiae RAD14 gene is a homolog of the XP-A correcting (XPAC) gene...
  9. Kim N, Jinks Robertson S. Abasic sites in the transcribed strand of yeast DNA are removed by transcription-coupled nucleotide excision repair. Mol Cell Biol. 2010;30:3206-15 pubmed publisher
    ..Such transcription-coupled NER of AP sites may explain previously suggested links between the BER pathway and transcription. ..

More Information


  1. Scott A, Neishabury M, Jones D, Reed S, Boiteux S, Waters R. Spontaneous mutation, oxidative DNA damage, and the roles of base and nucleotide excision repair in the yeast Saccharomyces cerevisiae. Yeast. 1999;15:205-18 pubmed
    ..of forward mutation to canavanine resistance in mutants defective in the nucleotide excision repair (NER) gene RAD14. This was not increased further in strains additionally defective in OGG1...
  2. Lehner K, Jinks Robertson S. The mismatch repair system promotes DNA polymerase zeta-dependent translesion synthesis in yeast. Proc Natl Acad Sci U S A. 2009;106:5749-54 pubmed publisher
    ..Finally, in contrast to its ability to remove mistakes made by replicative DNA polymerases, we show that MMR fails to efficiently correct errors introduced by Polzeta. ..
  3. Guo D, Xie Z, Shen H, Zhao B, Wang Z. Translesion synthesis of acetylaminofluorene-dG adducts by DNA polymerase zeta is stimulated by yeast Rev1 protein. Nucleic Acids Res. 2004;32:1122-30 pubmed
    ..Consistent with the in vitro results, both Polzeta and Rev1 were found to be equally important for error-prone translesion synthesis across from AAF-dG DNA adducts in yeast cells...
  4. Collura A, Kemp P, Boiteux S. Abasic sites linked to dUTP incorporation in DNA are a major cause of spontaneous mutations in absence of base excision repair and Rad17-Mec3-Ddc1 (9-1-1) DNA damage checkpoint clamp in Saccharomyces cerevisiae. DNA Repair (Amst). 2012;11:294-303 pubmed publisher
  5. Yu Y, Deng Y, Reed S, Millar C, Waters R. Histone variant Htz1 promotes histone H3 acetylation to enhance nucleotide excision repair in Htz1 nucleosomes. Nucleic Acids Res. 2013;41:9006-19 pubmed publisher
    ..Consequently, this results in an increased binding of a NER protein, Rad14, to damaged DNA...
  6. Lee S, Yu S, Prakash L, Prakash S. Yeast RAD26, a homolog of the human CSB gene, functions independently of nucleotide excision repair and base excision repair in promoting transcription through damaged bases. Mol Cell Biol. 2002;22:4383-9 pubmed
    ..of the GAL2, GAL7, and GAL10 genes is reduced in MMS-treated rad26 Delta cells and also in mag1 Delta rad14 Delta cells, whereas a very severe reduction in transcription occurs in MMS-treated mag1 Delta rad14 Delta rad26 ..
  7. Halmai M, Frittmann O, Szabo Z, Daraba A, Gali V, Balint E, et al. Mutations at the Subunit Interface of Yeast Proliferating Cell Nuclear Antigen Reveal a Versatile Regulatory Domain. PLoS ONE. 2016;11:e0161307 pubmed publisher
  8. Guzder S, Sung P, Prakash L, Prakash S. Nucleotide excision repair in yeast is mediated by sequential assembly of repair factors and not by a pre-assembled repairosome. J Biol Chem. 1996;271:8903-10 pubmed
    ..immunoprecipitation, and gel filtration of homogeneous proteins, we show that the damage recognition factor Rad14 exists in a ternary complex with the Rad1-Rad10 nuclease...
  9. Gangavarapu V, Santa Maria S, Prakash S, Prakash L. Requirement of replication checkpoint protein kinases Mec1/Rad53 for postreplication repair in yeast. MBio. 2011;2:e00079-11 pubmed publisher
    ..We discuss this important issue and suggest that lesion bypass in Saccharomyces cerevisiae cells occurs in conjunction with the stalled replication forks and not in gaps. ..
  10. Giannattasio M, Lazzaro F, Longhese M, Plevani P, Muzi Falconi M. Physical and functional interactions between nucleotide excision repair and DNA damage checkpoint. EMBO J. 2004;23:429-38 pubmed
    ..in the response to methyl methane sulfonate and double-strand breaks, we isolated cdu1-1, which is allelic to RAD14, the homolog of human XPA, involved in lesion recognition during nucleotide excision repair (NER)...
  11. Sharma N, Kochenova O, Shcherbakova P. The non-canonical protein binding site at the monomer-monomer interface of yeast proliferating cell nuclear antigen (PCNA) regulates the Rev1-PCNA interaction and Pol?/Rev1-dependent translesion DNA synthesis. J Biol Chem. 2011;286:33557-66 pubmed publisher
    ..The new mode of Rev1-PCNA binding described here suggests a mechanism by which Rev1 adopts a catalytically inactive configuration at the replication fork. ..
  12. Simon N, Ebert C, Schneider S. Structural Basis for Bulky-Adduct DNA-Lesion Recognition by the Nucleotide Excision Repair Protein Rad14. Chemistry. 2016;22:10782-5 pubmed publisher
    ..and acetylaminopyrenyl, as well as their crystal structures in complex with the yeast XPA homologue Rad14. This work further substantiates the indirect lesion-detection mechanism employed by the NER system that ..
  13. Rodriguez K, Talamantez J, Huang W, Reed S, Wang Z, Chen L, et al. Affinity purification and partial characterization of a yeast multiprotein complex for nucleotide excision repair using histidine-tagged Rad14 protein. J Biol Chem. 1998;273:34180-9 pubmed
    ..this model further, we have constructed a histidine-tagged version of the yeast DNA damage recognition protein Rad14. Affinity purification of this protein from yeast nuclear extracts resulted in the co-purification of Rad1, Rad7, ..
  14. Revers L, Cardone J, Bonatto D, Saffi J, Grey M, Feldmann H, et al. Thermoconditional modulation of the pleiotropic sensitivity phenotype by the Saccharomyces cerevisiae PRP19 mutant allele pso4-1. Nucleic Acids Res. 2002;30:4993-5003 pubmed
  15. Fedorova I, Kovaltzova S, Gracheva L, Evstuhina T, Korolev V. Requirement of HSM3 gene for spontaneous mutagenesis in Saccharomyces cerevisiae. Mutat Res. 2004;554:67-75 pubmed
    ..Effect of accumulation of spontaneous mutants was observed in slowly dividing cells in the rad1, rad2, rad14, rad54, and pms1, but it was absent in the rev3, pol2 and pol3 mutants...
  16. Koch S, Kuper J, Gasteiger K, Simon N, Strasser R, Eisen D, et al. Structural insights into the recognition of cisplatin and AAF-dG lesion by Rad14 (XPA). Proc Natl Acad Sci U S A. 2015;112:8272-7 pubmed publisher
    ..Here we present two crystal structures of the DNA binding domain (DBD) of the yeast XPA homolog Rad14 bound to DNA with either a cisplatin lesion (1,2-GG) or an acetylaminofluorene adduct (AAF-dG)...
  17. Guillet M, van der Kemp P, Boiteux S. dUTPase activity is critical to maintain genetic stability in Saccharomyces cerevisiae. Nucleic Acids Res. 2006;34:2056-66 pubmed
    ..Therefore, the normal cellular metabolism, and not only its byproducts, is an important source of endogenous DNA damage and genetic instability in eukaryotic cells. ..
  18. Woudstra E, Gilbert C, Fellows J, Jansen L, Brouwer J, Erdjument Bromage H, et al. A Rad26-Def1 complex coordinates repair and RNA pol II proteolysis in response to DNA damage. Nature. 2002;415:929-33 pubmed
  19. McA Nulty M, Lippard S. The HMG-domain protein Ixr1 blocks excision repair of cisplatin-DNA adducts in yeast. Mutat Res. 1996;362:75-86 pubmed
    ..in the ixr1 strain is the result of enhanced repair was investigated in rad1, rad2, rad4, rad6, rad9, rad10, rad14 and rad52 backgrounds...
  20. Monti P, Ciribilli Y, Russo D, Bisio A, Perfumo C, Andreotti V, et al. Rev1 and Polzeta influence toxicity and mutagenicity of Me-lex, a sequence selective N3-adenine methylating agent. DNA Repair (Amst). 2008;7:431-8 pubmed publisher
    ..yeast strain and in different DNA repair-deficient derivatives: the nucleotide excision repair (NER)-deficient rad14, and the BER-deficient mag1 or apn1apn2 strains...
  21. Haruta N, Kubota Y, Hishida T. Chronic low-dose ultraviolet-induced mutagenesis in nucleotide excision repair-deficient cells. Nucleic Acids Res. 2012;40:8406-15 pubmed
    ..Here, we investigated how chronic low-dose UV (CLUV)-induced mutagenesis occurs in rad14? NER-deficient yeast cells, which lack the yeast orthologue of human xeroderma pigmentosum A (XPA)...
  22. Guzder S, Bailly V, Sung P, Prakash L, Prakash S. Yeast DNA repair protein RAD23 promotes complex formation between transcription factor TFIIH and DNA damage recognition factor RAD14. J Biol Chem. 1995;270:8385-8 pubmed
    ..It has, however, remained unclear how TFIIH is assembled with the other damage recognition component RAD14. Here, we demonstrate a higher order complex consisting of TFIIH, RAD14, and another NER protein RAD23, and ..
  23. Game J, Kaufman P. Role of Saccharomyces cerevisiae chromatin assembly factor-I in repair of ultraviolet radiation damage in vivo. Genetics. 1999;151:485-97 pubmed
    ..We find an increased loss of telomeric gene silencing in rad6Delta cac1Delta and rad18Delta cac1Delta double mutants, suggesting that CAF-I and multiple factors in the postreplicative repair pathway influence chromosome structure. ..
  24. Santa Maria S, Gangavarapu V, Johnson R, Prakash L, Prakash S. Requirement of Nse1, a subunit of the Smc5-Smc6 complex, for Rad52-dependent postreplication repair of UV-damaged DNA in Saccharomyces cerevisiae. Mol Cell Biol. 2007;27:8409-18 pubmed
    ..We discuss the possible ways by which the Smc5-Smc6 complex, including its associated ubiquitin ligase and SUMO ligase activities, might contribute to the Rad52-dependent nonrecombinational and recombinational modes of PRR. ..
  25. Jansen L, den Dulk H, Brouns R, de Ruijter M, Brandsma J, Brouwer J. Spt4 modulates Rad26 requirement in transcription-coupled nucleotide excision repair. EMBO J. 2000;19:6498-507 pubmed
    ..Our findings suggest that Rad26 acts as an elongation factor rendering transcription TCR competent and that its requirement can be modulated by Spt4. ..
  26. Branzei D, Seki M, Onoda F, Enomoto T. The product of Saccharomyces cerevisiae WHIP/MGS1, a gene related to replication factor C genes, interacts functionally with DNA polymerase delta. Mol Genet Genomics. 2002;268:371-86 pubmed
    ..Possible roles of Mgs1, DNA polymerase delta, Rad18 and Mms2 in replication and replication fork restart are discussed. ..
  27. Boiteux S, Guillet M. Use of yeast for detection of endogenous abasic lesions, their source, and their repair. Methods Enzymol. 2006;408:79-91 pubmed
    ..The second assay uses the apn1 apn2 rad14 triple mutant, which is viable but exhibits a spontaneous mutator phenotype...
  28. Abdulovic A, Minesinger B, Jinks Robertson S. Identification of a strand-related bias in the PCNA-mediated bypass of spontaneous lesions by yeast Poleta. DNA Repair (Amst). 2007;6:1307-18 pubmed
    ..Our results suggest that there is a polymerase hierarchy between Poleta and Polzeta in the bypass of certain lesions and that the interaction of Poleta with PCNA is needed for some, but not all, spontaneous lesion bypass. ..
  29. Yu S, Lee S, Johnson R, Prakash L, Prakash S. The stalling of transcription at abasic sites is highly mutagenic. Mol Cell Biol. 2003;23:382-8 pubmed
    ..From the various observations presented here, we infer that the stalling of transcription at AP sites is highly mutagenic. ..
  30. Hanway D, Chin J, Xia G, Oshiro G, Winzeler E, Romesberg F. Previously uncharacterized genes in the UV- and MMS-induced DNA damage response in yeast. Proc Natl Acad Sci U S A. 2002;99:10605-10 pubmed
    ..Epistatsis analysis of four of the genes was performed to determine the DNA damage repair pathways in which the protein products function. ..
  31. Sarangi P, Bartosova Z, Altmannova V, Holland C, Chavdarova M, Lee S, et al. Sumoylation of the Rad1 nuclease promotes DNA repair and regulates its DNA association. Nucleic Acids Res. 2014;42:6393-404 pubmed publisher
    ..These findings suggest a model whereby sumoylation of Rad1 promotes its disengagement from DNA after nuclease cleavage, allowing it to efficiently attend to large numbers of DNA lesions. ..
  32. Scheller J, Schürer A, Rudolph C, Hettwer S, Kramer W. MPH1, a yeast gene encoding a DEAH protein, plays a role in protection of the genome from spontaneous and chemically induced damage. Genetics. 2000;155:1069-81 pubmed
    ..Epistasis analyses were carried out with representative mutants from various repair pathways (msh6, mag1, apn1, rad14, rad52, rad6, mms2, and rev3)...
  33. Brocas C, Charbonnier J, Dherin C, Gangloff S, Maloisel L. Stable interactions between DNA polymerase ? catalytic and structural subunits are essential for efficient DNA repair. DNA Repair (Amst). 2010;9:1098-111 pubmed publisher
    ..Taken together, our data highlight a stringent dependence on Pol ? complex stability in DNA repair. ..
  34. Schürer K, Rudolph C, Ulrich H, Kramer W. Yeast MPH1 gene functions in an error-free DNA damage bypass pathway that requires genes from Homologous recombination, but not from postreplicative repair. Genetics. 2004;166:1673-86 pubmed
    ..On the contrary, in an sgs1 background we found a pronounced hyperrecombination phenotype. Thus, we propose that MPH1 is involved in a branch of homologous recombination that is specifically dedicated to error-free bypass. ..
  35. Bertrand P, Tishkoff D, Filosi N, Dasgupta R, Kolodner R. Physical interaction between components of DNA mismatch repair and nucleotide excision repair. Proc Natl Acad Sci U S A. 1998;95:14278-83 pubmed
    ..Saccharomyces cerevisiae RAD14 was identified in a two-hybrid screen using MSH2 as "bait," and pairwise interactions between MSH2 and ..
  36. Grogan D, Jinks Robertson S. Formaldehyde-induced mutagenesis in Saccharomyces cerevisiae: molecular properties and the roles of repair and bypass systems. Mutat Res. 2012;731:92-8 pubmed publisher
    ..Brief exposure to HCHO was mutagenic for NER-defective rad14 strains but not for a corresponding RAD14 strain, nor for a rad14 strain lacking both Pol? and Pol? TLS ..
  37. Stone J, Lujan S, Kunkel T. DNA polymerase zeta generates clustered mutations during bypass of endogenous DNA lesions in Saccharomyces cerevisiae. Environ Mol Mutagen. 2012;53:777-86 pubmed publisher
    ..Compared to NER-proficient strains, NER-deficient rad14Δ strains have elevated mutation rates that depend on Pol ζ...
  38. Chaurasia P, Sen R, Bhaumik S. Functional analysis of Rad14p, a DNA damage recognition factor in nucleotide excision repair, in regulation of transcription in vivo. J Biol Chem. 2013;288:793-806 pubmed publisher
    b>Rad14p is a DNA damage recognition factor in nucleotide excision repair. Intriguingly, we show here that Rad14p associates with the promoter of a galactose-inducible GAL1 gene after transcriptional induction in the absence of DNA lesion...
  39. Gellon L, Barbey R, Auffret van der Kemp P, Thomas D, Boiteux S. Synergism between base excision repair, mediated by the DNA glycosylases Ntg1 and Ntg2, and nucleotide excision repair in the removal of oxidatively damaged DNA bases in Saccharomyces cerevisiae. Mol Genet Genomics. 2001;265:1087-96 pubmed
    ..does not result in a spontaneous mutator phenotype, whereas simultaneous inactivation of Ntglp, Ntg2p and Radlp or Rad14p, both of which are involved in nucleotide excision repair (NER), does...
  40. Conde F, San Segundo P. Role of Dot1 in the response to alkylating DNA damage in Saccharomyces cerevisiae: regulation of DNA damage tolerance by the error-prone polymerases Polzeta/Rev1. Genetics. 2008;179:1197-210 pubmed publisher
    ..DOT1 partially or totally suppresses the MMS sensitivity of various DNA repair mutants (rad52, rad54, yku80, rad1, rad14, apn1, rad5, rad30)...
  41. Owiti N, Lopez C, Singh S, Stephenson A, Kim N. Def1 and Dst1 play distinct roles in repair of AP lesions in highly transcribed genomic regions. DNA Repair (Amst). 2017;55:31-39 pubmed publisher
    ..Overall, Def1 and Dst1 mediate very different outcomes in response to AP-induced transcription arrest. ..