Gene Symbol: Puf6p
Description: Puf6p
Alias: Puf6p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Gu W, Deng Y, Zenklusen D, Singer R. A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization. Genes Dev. 2004;18:1452-65 pubmed
    ..One of these proteins was Puf6p, a new member of the PUF family of highly conserved RNA-binding proteins such as Pumilio in Drosophila, ..
  2. Lee I, Li Z, Marcotte E. An improved, bias-reduced probabilistic functional gene network of baker's yeast, Saccharomyces cerevisiae. PLoS ONE. 2007;2:e988 pubmed
    ..YeastNet is available from http://www.yeastnet.org. ..
  3. Komili S, Farny N, Roth F, Silver P. Functional specificity among ribosomal proteins regulates gene expression. Cell. 2007;131:557-71 pubmed
    ..Together, our data indicate complex specialization of ribosomal proteins for specific cellular processes and support the existence of a ribosomal code. ..
  4. Deng Y, Singer R, Gu W. Translation of ASH1 mRNA is repressed by Puf6p-Fun12p/eIF5B interaction and released by CK2 phosphorylation. Genes Dev. 2008;22:1037-50 pubmed publisher
    ..b>Puf6p, an ASH1 mRNA-binding protein, has been implicated in this process as a translational repressor, but the ..
  5. Shen Z, Paquin N, Forget A, Chartrand P. Nuclear shuttling of She2p couples ASH1 mRNA localization to its translational repression by recruiting Loc1p and Puf6p. Mol Biol Cell. 2009;20:2265-75 pubmed publisher
    ..We find that She2p interacts with both Loc1p and Puf6p and that excluding She2p from the nucleus decreases this interaction...
  6. Peng W, Krogan N, Richards D, Greenblatt J, Hughes T. ESF1 is required for 18S rRNA synthesis in Saccharomyces cerevisiae. Nucleic Acids Res. 2004;32:1993-9 pubmed
    ..These data indicate that Esf1p plays a direct role in early pre-rRNA processing. ..
  7. McDonald R, Guilinger J, Mukherji S, Curtis E, Lee W, Liu D. Electrophilic activity-based RNA probes reveal a self-alkylating RNA for RNA labeling. Nat Chem Biol. 2014;10:1049-54 pubmed publisher
    ..This strategy enabled up to 500-fold enrichment of target RNA from total mammalian RNA or from cell lysate. We demonstrated the utility of this approach by selectively capturing proteins in yeast cell lysate that bind the ASH1 mRNA. ..
  8. Zhang Q, Meng X, Li D, Chen S, Luo J, Zhu L, et al. Binding of DEAD-box helicase Dhh1 to the 5'-untranslated region of ASH1 mRNA represses localized translation of ASH1 in yeast cells. J Biol Chem. 2017;292:9787-9800 pubmed publisher
  9. Li Z, Lee I, Moradi E, Hung N, Johnson A, Marcotte E. Rational extension of the ribosome biogenesis pathway using network-guided genetics. PLoS Biol. 2009;7:e1000213 pubmed publisher

More Information


  1. Qiu C, McCann K, Wine R, Baserga S, Hall T. A divergent Pumilio repeat protein family for pre-rRNA processing and mRNA localization. Proc Natl Acad Sci U S A. 2014;111:18554-9 pubmed publisher
    ..Thus, PUM repeats can be assembled into alternative folds that bind to structured nucleic acids in addition to forming canonical eight-repeat crescent-shaped RNA-binding domains found in classical PUF proteins. ..
  2. Shahbabian K, Jeronimo C, Forget A, Robert F, Chartrand P. Co-transcriptional recruitment of Puf6 by She2 couples translational repression to mRNA localization. Nucleic Acids Res. 2014;42:8692-704 pubmed publisher
  3. Yang Y, Ting Y, Liang K, Lo K. The Roles of Puf6 and Loc1 in 60S Biogenesis Are Interdependent, and Both Are Required for Efficient Accommodation of Rpl43. J Biol Chem. 2016;291:19312-23 pubmed publisher
    ..Additionally, the recruitment of Rpl43 is required for the release of Puf6 and Loc1. We propose that Puf6 and Loc1 facilitate Rpl43 loading and are sequentially released from 60S after incorporation of Rpl43 into ribosomes in yeast. ..