Gene Symbol: PUF4
Alias: YGL023, Puf4p
Species: Saccharomyces cerevisiae S288c
Chritton J, Wickens M. Translational repression by PUF proteins in vitro. RNA. 2010;16:1217-25 pubmed publisher
..We suggest a model in which PUF proteins can control translation termination or elongation. ..
Gerber A, Herschlag D, Brown P. Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast. PLoS Biol. 2004;2:E79 pubmed
..mitochondrial proteins; Puf1p and Puf2p interact preferentially with mRNAs encoding membrane-associated proteins; Puf4p preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors; and Puf5p is associated with mRNAs ..
Kennedy B, Gotta M, Sinclair D, Mills K, McNabb D, Murthy M, et al
. Redistribution of silencing proteins from telomeres to the nucleolus is associated with extension of life span in S. cerevisiae. Cell. 1997;89:381-91 pubmed
..this relocalization is dependent on both UTH4 a novel yeast gene that extends life span, and its homologue YGL023. Strikingly, the Sir complex is relocalized from telomeres to the nucleolus in old wild-type cells...
Olivas W, Parker R. The Puf3 protein is a transcript-specific regulator of mRNA degradation in yeast. EMBO J. 2000;19:6602-11 pubmed
..the role of five members of the Puf family present in the yeast genome (referred to as JSN1/PUF1, PUF2, PUF3, PUF4 and MPT5/PUF5). Yeast strains lacking all five Puf proteins showed differential expression of numerous yeast mRNAs...
Hook B, Goldstrohm A, Seay D, Wickens M. Two yeast PUF proteins negatively regulate a single mRNA. J Biol Chem. 2007;282:15430-8 pubmed
..single mRNA in Saccharomyces cerevisiae, encoding the HO endonuclease, is regulated by two distinct PUF proteins, Puf4p and Mpt5p. These proteins bind to adjacent sites and can co-occupy the mRNA...
Russo J, Olivas W. Conditional regulation of Puf1p, Puf4p, and Puf5p activity alters YHB1 mRNA stability for a rapid response to toxic nitric oxide stress in yeast. Mol Biol Cell. 2015;26:1015-29 pubmed publisher
..Here we show that YHB1 mRNA is destabilized by Puf1p, Puf4p, and Puf5p through two overlapping Puf recognition elements (PREs) in the YHB1 3' UTR...
Miller M, Higgin J, Hall T. Basis of altered RNA-binding specificity by PUF proteins revealed by crystal structures of yeast Puf4p. Nat Struct Mol Biol. 2008;15:397-402 pubmed publisher
..Some PUF proteins, including yeast Puf4p, have altered RNA binding specificity and use their eight repeats to bind to RNA sequences with nine or ten bases...
Cridge A, Castelli L, Smirnova J, Selley J, Rowe W, Hubbard S, et al
. Identifying eIF4E-binding protein translationally-controlled transcripts reveals links to mRNAs bound by specific PUF proteins. Nucleic Acids Res. 2010;38:8039-50 pubmed publisher
..We used affinity chromatography experiments to uncover specific RNA-stabilized complexes formed between Caf20p and Puf4p/Puf5p and between Eap1p and Puf1p/Puf2p...
Yosefzon Y, Koh Y, Chritton J, Lande A, Leibovich L, Barziv L, et al
. Divergent RNA binding specificity of yeast Puf2p. RNA. 2011;17:1479-88 pubmed publisher
..This study expands the repertoire of cis elements bound by PUF proteins and suggests new modes by which PUF proteins recognize their mRNA targets. ..