Gene Symbol: PUF3
Description: mRNA-binding protein PUF3
Alias: mRNA-binding protein PUF3
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Olivas W, Parker R. The Puf3 protein is a transcript-specific regulator of mRNA degradation in yeast. EMBO J. 2000;19:6602-11 pubmed
    ..we analyzed the role of five members of the Puf family present in the yeast genome (referred to as JSN1/PUF1, PUF2, PUF3, PUF4 and MPT5/PUF5)...
  2. Sheth U, Parker R. Decapping and decay of messenger RNA occur in cytoplasmic processing bodies. Science. 2003;300:805-8 pubmed
    ..These results define the flux of mRNAs between polysomes and P bodies as a critical aspect of cytoplasmic mRNA metabolism and a possible site for regulation of mRNA degradation...
  3. Saint Georges Y, Garcia M, Delaveau T, Jourdren L, Le Crom S, Lemoine S, et al. Yeast mitochondrial biogenesis: a role for the PUF RNA-binding protein Puf3p in mRNA localization. PLoS ONE. 2008;3:e2293 pubmed publisher
    ..have a Puf3p binding motif in their 3'UTR region and many of them have their MLR properties deeply affected by PUF3 deletion. Conversely, mutations in the Puf3p binding motif alter the mitochondrial localization of BCS1 mRNA...
  4. Chatenay Lapointe M, Shadel G. Repression of mitochondrial translation, respiration and a metabolic cycle-regulated gene, SLF1, by the yeast Pumilio-family protein Puf3p. PLoS ONE. 2011;6:e20441 pubmed publisher
    ..Altogether, these results should facilitate future studies on which of the many functions of Puf3p is most relevant for regulating mitochondrial gene expression and the role of nuclear-mitochondrial communication in aging and longevity. ..
  5. Zhu D, Stumpf C, Krahn J, Wickens M, Hall T. A 5' cytosine binding pocket in Puf3p specifies regulation of mitochondrial mRNAs. Proc Natl Acad Sci U S A. 2009;106:20192-7 pubmed publisher
    A single regulatory protein can control the fate of many mRNAs with related functions. The Puf3 protein of Saccharomyces cerevisiae is exemplary, as it binds and regulates more than 100 mRNAs that encode proteins with mitochondrial ..
  6. Foat B, Houshmandi S, Olivas W, Bussemaker H. Profiling condition-specific, genome-wide regulation of mRNA stability in yeast. Proc Natl Acad Sci U S A. 2005;102:17675-80 pubmed
    ..We provide computational and experimental evidence that regulation of mRNA stability by these factors is modulated in response to a variety of environmental stimuli. ..
  7. García Rodríguez L, Gay A, Pon L. Puf3p, a Pumilio family RNA binding protein, localizes to mitochondria and regulates mitochondrial biogenesis and motility in budding yeast. J Cell Biol. 2007;176:197-207 pubmed
    ..We find that Puf3p localizes to the cytosolic face of the mitochondrial outer membrane. Overexpression of PUF3 results in reduced mitochondrial respiratory activity and reduced levels of Pet123p, a protein encoded by a Puf3p-..
  8. Eliyahu E, Pnueli L, Melamed D, Scherrer T, Gerber A, Pines O, et al. Tom20 mediates localization of mRNAs to mitochondria in a translation-dependent manner. Mol Cell Biol. 2010;30:284-94 pubmed publisher
    ..We therefore constructed a tom20 Delta puf3 Delta double-knockout strain, which demonstrated growth defects under conditions where fully functional ..
  9. Jiang H, Guan W, Gu Z. Tinkering evolution of post-transcriptional RNA regulons: puf3p in fungi as an example. PLoS Genet. 2010;6:e1001030 pubmed publisher
    ..Our results provide a scenario for how evolution like a tinker exploits pre-existing materials of a conserved post-transcriptional regulon to regulate gene expression for novel functional roles. ..

More Information


  1. Ulbricht R, Olivas W. Puf1p acts in combination with other yeast Puf proteins to control mRNA stability. RNA. 2008;14:246-62 pubmed
  2. Lee D, Ohn T, Chiang Y, Quigley G, Yao G, Liu Y, et al. PUF3 acceleration of deadenylation in vivo can operate independently of CCR4 activity, possibly involving effects on the PAB1-mRNP structure. J Mol Biol. 2010;399:562-75 pubmed publisher
    ..We have investigated the mechanisms by which PUF3 in Saccharomyces cerevisiae accelerates deadenylation of the COX17 mRNA...
  3. Yosefzon Y, Koh Y, Chritton J, Lande A, Leibovich L, Barziv L, et al. Divergent RNA binding specificity of yeast Puf2p. RNA. 2011;17:1479-88 pubmed publisher
    ..This study expands the repertoire of cis elements bound by PUF proteins and suggests new modes by which PUF proteins recognize their mRNA targets. ..
  4. Miller M, Russo J, Fischer A, Lopez Leban F, Olivas W. Carbon source-dependent alteration of Puf3p activity mediates rapid changes in the stabilities of mRNAs involved in mitochondrial function. Nucleic Acids Res. 2014;42:3954-70 pubmed publisher
    ..In addition, the activity of Puf3p is rapidly altered by changing the carbon source. PUF3 expression is not decreased at the RNA or protein level by different carbon sources and localization is not ..
  5. Houshmandi S, Olivas W. Yeast Puf3 mutants reveal the complexity of Puf-RNA binding and identify a loop required for regulation of mRNA decay. RNA. 2005;11:1655-66 pubmed
    ..Finally, we identified an outer surface loop that was dispensable for binding but was required to promote both rapid deadenylation and subsequent decapping of the COX17 mRNA, most likely as a point of protein-protein interactions. ..
  6. Rowe W, Kershaw C, Castelli L, Costello J, Ashe M, Grant C, et al. Puf3p induces translational repression of genes linked to oxidative stress. Nucleic Acids Res. 2014;42:1026-41 pubmed publisher
    ..Under oxidative stress, a PUF3 deleted strain exhibits more robust growth than wild-type cells and the shift in translation from polysomes to ..