Gene Symbol: PTP2
Description: tyrosine protein phosphatase PTP2
Alias: tyrosine protein phosphatase PTP2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Wurgler Murphy S, Maeda T, Witten E, Saito H. Regulation of the Saccharomyces cerevisiae HOG1 mitogen-activated protein kinase by the PTP2 and PTP3 protein tyrosine phosphatases. Mol Cell Biol. 1997;17:1289-97 pubmed
    ..A catalytically inactive Cys-to-Ser Ptp2p mutant (Ptp2(C/S)p) is tightly bound to tyrosine-phosphorylated Hog1p in vivo...
  2. Posas F, Wurgler Murphy S, Maeda T, Witten E, Thai T, Saito H. Yeast HOG1 MAP kinase cascade is regulated by a multistep phosphorelay mechanism in the SLN1-YPD1-SSK1 "two-component" osmosensor. Cell. 1996;86:865-75 pubmed
    ..We propose that the multistep phosphorelay mechanism is a universal signal transduction apparatus utilized both in prokaryotes and eukaryotes. ..
  3. Torres Quiroz F, García Marqués S, Coria R, Randez Gil F, Prieto J. The activity of yeast Hog1 MAPK is required during endoplasmic reticulum stress induced by tunicamycin exposure. J Biol Chem. 2010;285:20088-96 pubmed publisher
    ..Hence, our results describe new aspects of the yeast response to ER stress and identify additional functions of glycerol and the Hog1p MAPK to provide stress resistance. ..
  4. Maeda T, Wurgler Murphy S, Saito H. A two-component system that regulates an osmosensing MAP kinase cascade in yeast. Nature. 1994;369:242-5 pubmed
    ..Here we describe a two-component system in Saccharomyces cerevisiae that regulates an osmosensing MAP kinase cascade. ..
  5. Yang H, Tatebayashi K, Yamamoto K, Saito H. Glycosylation defects activate filamentous growth Kss1 MAPK and inhibit osmoregulatory Hog1 MAPK. EMBO J. 2009;28:1380-91 pubmed publisher
    ..Conversely, we now show that glycosylation defects activate only Kss1, while activated Kss1 and the Ptp2 tyrosine phosphatase inhibit Hog1. In the absence of Kss1 or Ptp2, however, glycosylation defects activate Hog1...
  6. Murakami Y, Tatebayashi K, Saito H. Two adjacent docking sites in the yeast Hog1 mitogen-activated protein (MAP) kinase differentially interact with the Pbs2 MAP kinase kinase and the Ptp2 protein tyrosine phosphatase. Mol Cell Biol. 2008;28:2481-94 pubmed publisher
    ..specifically interacts with its activator, the MAPK kinase Pbs2, and its major inactivator, the protein phosphatase Ptp2. We found, in the N-terminal noncatalytic region of Pbs2, a specific Hog1-binding domain, termed HBD-1...
  7. Hermansyah -, Laviña W, Sugiyama M, Kaneko Y, Harashima S. Identification of protein kinase disruptions as suppressors of the calcium sensitivity of S. cerevisiae Deltaptp2 Deltamsg5 protein phosphatase double disruptant. Arch Microbiol. 2010;192:157-65 pubmed publisher
    The double disruptant of the S. cerevisiae protein phosphatase (PPase) genes, PTP2 (phosphotyrosine-specific PPase) and MSG5 (phosphotyrosine and phosphothreonine/serine-PPase) causes calcium-sensitive growth (Cas)...
  8. Shen Z, Li Y, Gasparski A, Abeliovich H, Greenberg M. Cardiolipin Regulates Mitophagy through the Protein Kinase C Pathway. J Biol Chem. 2017;292:2916-2923 pubmed publisher
    ..These findings indicate that loss of CL impairs MAPK pathway activation, and decreased activation of the PKC pathway leads to defective mitophagy. ..
  9. Vendrell A, Martínez Pastor M, Gonzalez Novo A, Pascual Ahuir A, Sinclair D, Proft M, et al. Sir2 histone deacetylase prevents programmed cell death caused by sustained activation of the Hog1 stress-activated protein kinase. EMBO Rep. 2011;12:1062-8 pubmed publisher
    ..Sir2 is involved in protection against Hog1-induced cell death and can suppress Hog1-induced ROS accumulation. Therefore, cell death seems to be dictated by the balance of ROS induced by Hog1 and the protective effect of Sir2. ..

More Information


  1. Winkler A, Arkind C, Mattison C, Burkholder A, Knoche K, Ota I. Heat stress activates the yeast high-osmolarity glycerol mitogen-activated protein kinase pathway, and protein tyrosine phosphatases are essential under heat stress. Eukaryot Cell. 2002;1:163-73 pubmed
    ..It was also found that protein tyrosine phosphatases (PTPs) Ptp2 and Ptp3, which inactivate Hog1, have two functions during heat stress...
  2. Young C, Mapes J, Hanneman J, Al Zarban S, Ota I. Role of Ptc2 type 2C Ser/Thr phosphatase in yeast high-osmolarity glycerol pathway inactivation. Eukaryot Cell. 2002;1:1032-40 pubmed
    ..Here, we examined the function of Ptc2 and Ptc3. First, deletion of PTC2 and/or PTC3 together with PTP2, encoding the protein tyrosine phosphatase that inactivates Hog1, produced a strong growth defect at 37 degrees C ..
  3. Yamamoto K, Tatebayashi K, Tanaka K, Saito H. Dynamic control of yeast MAP kinase network by induced association and dissociation between the Ste50 scaffold and the Opy2 membrane anchor. Mol Cell. 2010;40:87-98 pubmed publisher
    ..Thus, dynamic regulation of Ste50-Opy2 interaction fine-tunes the MAPK signaling network. ..
  4. Zhan X, Deschenes R, Guan K. Differential regulation of FUS3 MAP kinase by tyrosine-specific phosphatases PTP2/PTP3 and dual-specificity phosphatase MSG5 in Saccharomyces cerevisiae. Genes Dev. 1997;11:1690-702 pubmed
    ..In contrast, disruption of PTP3 in combination with its homolog PTP2 results in constitutive tyrosine phosphorylation, enhanced kinase activity of Fus3p MAP kinase on stimulation, and ..
  5. Cheng A, Ross K, Kaldis P, Solomon M. Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases. Genes Dev. 1999;13:2946-57 pubmed
    ..Furthermore, PP2C-like enzymes are the predominant phosphatases toward human Cdk2 in HeLa cell extracts, indicating that the substrate specificity of PP2Cs toward CDKs is evolutionarily conserved. ..
  6. Zhan X, Hong Y, Zhu T, Mitchell A, Deschenes R, Guan K. Essential functions of protein tyrosine phosphatases PTP2 and PTP3 and RIM11 tyrosine phosphorylation in Saccharomyces cerevisiae meiosis and sporulation. Mol Biol Cell. 2000;11:663-76 pubmed
    ..Here we report that deletion of PTP2 and PTP3 results in a sporulation defect, suggesting that tyrosine phosphorylation is involved in regulation of ..
  7. Sato N, Kawahara H, Toh e A, Maeda T. Phosphorelay-regulated degradation of the yeast Ssk1p response regulator by the ubiquitin-proteasome system. Mol Cell Biol. 2003;23:6662-71 pubmed
    ..Our results indicate that unphosphorylated Ssk1p is selectively degraded by the Ubc7p-dependent ubiquitin-proteasome system and that this mechanism downregulates the HOG pathway after the completion of the osmotic adaptation. ..
  8. Park H, Beeser A, Clancy M, Cooper T. The S. cerevisiae nitrogen starvation-induced Yvh1p and Ptp2p phosphatases play a role in control of sporulation. Yeast. 1996;12:1135-51 pubmed
    ..We also observed that expression of the PTP2 tyrosine phosphatase gene (a negative regulator of the osmosensing MAP kinase cascade), but not the PTP1 gene (also ..
  9. Adhikari H, Cullen P. Metabolic respiration induces AMPK- and Ire1p-dependent activation of the p38-Type HOG MAPK pathway. PLoS Genet. 2014;10:e1004734 pubmed publisher
    ..Thus, an evolutionarily conserved regulatory axis links metabolic respiration and AMPK to Ire1p, which regulates a differentiation response involving the modulated activity of ERK and p38 MAPK pathways. ..
  10. Maeda T, Tsai A, Saito H. Mutations in a protein tyrosine phosphatase gene (PTP2) and a protein serine/threonine phosphatase gene (PTC1) cause a synthetic growth defect in Saccharomyces cerevisiae. Mol Cell Biol. 1993;13:5408-17 pubmed
    Two protein tyrosine phosphatase genes, PTP1 and PTP2, are known in Saccharomyces cerevisiae...
  11. Aguilera J, Rodríguez Vargas S, Prieto J. The HOG MAP kinase pathway is required for the induction of methylglyoxal-responsive genes and determines methylglyoxal resistance in Saccharomyces cerevisiae. Mol Microbiol. 2005;56:228-39 pubmed
    ..Moreover, hyperactivation of Hog1p by deletion of protein phosphatase PTP2 enhanced the response, while blocking the pathway by deletion of the MAPKK PBS2 had a negative effect...
  12. Hahn J, Thiele D. Regulation of the Saccharomyces cerevisiae Slt2 kinase pathway by the stress-inducible Sdp1 dual specificity phosphatase. J Biol Chem. 2002;277:21278-84 pubmed
    ..Negative regulation of Slt2 is achieved via dephosphorylation by two protein-tyrosine phosphatases, Ptp2 and Ptp3, and a dual specificity phosphatase, Msg5...
  13. Hermansyah -, Sugiyama M, Kaneko Y, Harashima S. Yeast protein phosphatases Ptp2p and Msg5p are involved in G1-S transition, CLN2 transcription, and vacuole morphogenesis. Arch Microbiol. 2009;191:721-33 pubmed publisher
    We previously reported that double disruption of protein phosphatase (PPase) genes PTP2 (phosphotyrosine-specific PPase) and MSG5 (phosphotyrosine and phosphothreonine/serine-PPase) causes Ca(2+) sensitive growth, whereas the single ..
  14. Thorne T, Ho H, Huvet M, Haynes K, Stumpf M. Prediction of putative protein interactions through evolutionary analysis of osmotic stress response in the model yeast Saccharomyces cerevisae. Fungal Genet Biol. 2011;48:504-11 pubmed publisher
    ..We do find that correlated evolution provides some useful information for the prediction of protein-protein interactions, but that these alone are not sufficient to explain detectable patterns of correlated evolution. ..
  15. Laviña W, Hermansyah -, Sugiyama M, Kaneko Y, Harashima S. Functionally redundant protein phosphatase genes PTP2 and MSG5 co-regulate the calcium signaling pathway in Saccharomyces cerevisiae upon exposure to high extracellular calcium concentration. J Biosci Bioeng. 2013;115:138-46 pubmed publisher
    ..We have previously reported that the disruption of two protein phosphatase (PPase) genes, PTP2 and MSG5, causes calcium sensitivity indicating that functional redundancy exists between the two PPases in ..
  16. Mapes J, Ota I. Nbp2 targets the Ptc1-type 2C Ser/Thr phosphatase to the HOG MAPK pathway. EMBO J. 2004;23:302-11 pubmed
    ..The Ptc1-type 2C Ser/Thr phosphatase (PP2C) inactivates Hog1 by dephosphorylating phospho-Thr, while the Ptp2 and Ptp3 protein tyrosine phosphatases dephosphorylate phospho-Tyr...
  17. Mattison C, Ota I. Two protein tyrosine phosphatases, Ptp2 and Ptp3, modulate the subcellular localization of the Hog1 MAP kinase in yeast. Genes Dev. 2000;14:1229-35 pubmed
    ..Unexpectedly, a strain lacking the nuclear protein tyrosine phosphatase, Ptp2, showed decreased Hog1 nuclear retention, while a strain lacking the cytoplasmic Ptp3 showed prolonged Hog1 nuclear ..
  18. Li S, Diakov T, Rizzo J, Kane P. Vacuolar H+-ATPase works in parallel with the HOG pathway to adapt Saccharomyces cerevisiae cells to osmotic stress. Eukaryot Cell. 2012;11:282-91 pubmed publisher
    ..Together, these data suggest that the V-ATPase acts in parallel with the HOG pathway in order to mediate salt detoxification. ..
  19. Mattison C, Spencer S, Kresge K, Lee J, Ota I. Differential regulation of the cell wall integrity mitogen-activated protein kinase pathway in budding yeast by the protein tyrosine phosphatases Ptp2 and Ptp3. Mol Cell Biol. 1999;19:7651-60 pubmed
    ..In Saccharomyces cerevisiae, two PTPs, Ptp2 and Ptp3, inactivate the MAPKs, Hog1 and Fus3, with different specificities...
  20. Jacoby T, Flanagan H, Faykin A, Seto A, Mattison C, Ota I. Two protein-tyrosine phosphatases inactivate the osmotic stress response pathway in yeast by targeting the mitogen-activated protein kinase, Hog1. J Biol Chem. 1997;272:17749-55 pubmed
    ..Two genes encoding protein-tyrosine phosphatases, PTP2, and a new phosphatase, PTP3, have been isolated in a genetic selection for negative regulators of an osmotic ..