Gene Symbol: PTC2
Description: type 2C protein phosphatase PTC2
Alias: type 2C protein phosphatase PTC2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Marsolier M, Roussel P, Leroy C, Mann C. Involvement of the PP2C-like phosphatase Ptc2p in the DNA checkpoint pathways of Saccharomyces cerevisiae. Genetics. 2000;154:1523-32 pubmed
    ..searched for genes whose overexpression suppressed the toxicity of a dominant-lethal form of RAD53 and identified PTC2, which encodes a protein phosphatase of the PP2C family...
  2. Warmka J, Hanneman J, Lee J, Amin D, Ota I. Ptc1, a type 2C Ser/Thr phosphatase, inactivates the HOG pathway by dephosphorylating the mitogen-activated protein kinase Hog1. Mol Cell Biol. 2001;21:51-60 pubmed
    ..Three type 2C Ser/Thr phosphatases (PTCs), Ptc1, Ptc2, and Ptc3, have been isolated as negative regulators of this pathway...
  3. Young C, Mapes J, Hanneman J, Al Zarban S, Ota I. Role of Ptc2 type 2C Ser/Thr phosphatase in yeast high-osmolarity glycerol pathway inactivation. Eukaryot Cell. 2002;1:1032-40 pubmed
    ..b>Ptc2 and Ptc3 are 75% identical to each other and differ from Ptc1 in having a noncatalytic domain...
  4. Bazzi M, Mantiero D, Trovesi C, Lucchini G, Longhese M. Dephosphorylation of gamma H2A by Glc7/protein phosphatase 1 promotes recovery from inhibition of DNA replication. Mol Cell Biol. 2010;30:131-45 pubmed publisher
    ..inactivation during methylmethane sulfonate exposure, which instead requires the protein phosphatases Pph3, Ptc2, and Ptc3...
  5. Ohkuni K, Okuda A, Kikuchi A. Yeast Nap1-binding protein Nbp2p is required for mitotic growth at high temperatures and for cell wall integrity. Genetics. 2003;165:517-29 pubmed
  6. Travesa A, Duch A, Quintana D. Distinct phosphatases mediate the deactivation of the DNA damage checkpoint kinase Rad53. J Biol Chem. 2008;283:17123-30 pubmed publisher
    ..a persistent double strand break in G(2)/M has been shown to depend on the presence of the PP2C-type phosphatases Ptc2 and Ptc3...
  7. Welihinda A, Tirasophon W, Green S, Kaufman R. Protein serine/threonine phosphatase Ptc2p negatively regulates the unfolded-protein response by dephosphorylating Ire1p kinase. Mol Cell Biol. 1998;18:1967-77 pubmed
    ..Searching for proteins that interact with Ire1p in Saccharomyces cerevisiae, we isolated PTC2, which encodes a serine/threonine phosphatase of type 2C...
  8. Sakumoto N, Matsuoka I, Mukai Y, Ogawa N, Kaneko Y, Harashima S. A series of double disruptants for protein phosphatase genes in Saccharomyces cerevisiae and their phenotypic analysis. Yeast. 2002;19:587-99 pubmed
    ..3 M CaCl(2). The double msg5 ptc2 disruptant failed to grow on medium containing 1...
  9. Sharmin D, Sasano Y, Sugiyama M, Harashima S. Effects of deletion of different PP2C protein phosphatase genes on stress responses in Saccharomyces cerevisiae. Yeast. 2014;31:393-409 pubmed publisher
    ..However, several combinations exhibited novel phenotypes, e.g. the ?ptc5?ptc7 double disruptant and the ?ptc2?ptc3?ptc5?ptc7 quadruple disruptant exhibited low (13°C) and high (37°C) temperature-sensitive growth, ..

More Information


  1. Stirling P, Srayko M, Takhar K, Pozniakovsky A, Hyman A, Leroux M. Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeletal protein function and cell cycle progression. Mol Biol Cell. 2007;18:2336-45 pubmed
    ..Our data support a model in which Plp2p modulates the biogenesis of several CCT substrates relating to cell cycle and cytoskeletal function, which together contribute to the essential function of PLP2. ..
  2. Kim J, Hicks W, Li J, Tay S, Haber J. Protein phosphatases pph3, ptc2, and ptc3 play redundant roles in DNA double-strand break repair by homologous recombination. Mol Cell Biol. 2011;31:507-16 pubmed publisher
    ..Recovery involves two members of the protein phosphatase 2C (PP2C) family, Ptc2 and Ptc3, as well as the protein phosphatase 4 (PP4) enzyme, Pph3...
  3. Au T, Rodriguez J, Vincent J, Tsukiyama T. ATP-dependent chromatin remodeling factors tune S phase checkpoint activity. Mol Cell Biol. 2011;31:4454-63 pubmed publisher
    ..Based on these results, we propose that Isw2 and Ino80 are targeted to stalled replication forks via RPA and directly control the amplitude of S phase checkpoint activity and the subsequent deactivation process. ..
  4. Tsabar M, Waterman D, Aguilar F, Katsnelson L, Eapen V, Memisoglu G, et al. Asf1 facilitates dephosphorylation of Rad53 after DNA double-strand break repair. Genes Dev. 2016;30:1211-24 pubmed publisher
  5. O Neill B, Szyjka S, Lis E, Bailey A, Yates J, Aparicio O, et al. Pph3-Psy2 is a phosphatase complex required for Rad53 dephosphorylation and replication fork restart during recovery from DNA damage. Proc Natl Acad Sci U S A. 2007;104:9290-5 pubmed
    ..These findings suggest that Rad53 regulates replication fork restart and initiation of late firing origins independently and that regulation of these processes is mediated by specific Rad53 phosphatases. ..
  6. Shitamukai A, Hirata D, Sonobe S, Miyakawa T. Evidence for antagonistic regulation of cell growth by the calcineurin and high osmolarity glycerol pathways in Saccharomyces cerevisiae. J Biol Chem. 2004;279:3651-61 pubmed
  7. Munoz I, Simon E, Casals N, Clotet J, Arino J. Identification of multicopy suppressors of cell cycle arrest at the G1-S transition in Saccharomyces cerevisiae. Yeast. 2003;20:157-69 pubmed
    ..The possible relationship between the known functions of these suppressor genes and the progress through the G(1)-S transition is discussed. ..
  8. Guillemain G, Ma E, Mauger S, Miron S, Thai R, Guerois R, et al. Mechanisms of checkpoint kinase Rad53 inactivation after a double-strand break in Saccharomyces cerevisiae. Mol Cell Biol. 2007;27:3378-89 pubmed
    ..We have previously provided evidence that the phosphatases Ptc2 and Ptc3 of the protein phosphatase 2C type are required for DNA checkpoint inactivation after a DSB and probably ..
  9. Szyjka S, Aparicio J, Viggiani C, Knott S, Xu W, Tavare S, et al. Rad53 regulates replication fork restart after DNA damage in Saccharomyces cerevisiae. Genes Dev. 2008;22:1906-20 pubmed publisher
    ..Furthermore, combined deletion of PPH3 and PTC2, a second, unrelated Rad53 phosphatase, results in complete replication fork arrest and lethality in MMS, ..
  10. Aucher W, Becker E, Ma E, Miron S, Martel A, Ochsenbein F, et al. A strategy for interaction site prediction between phospho-binding modules and their partners identified from proteomic data. Mol Cell Proteomics. 2010;9:2745-59 pubmed publisher
    ..The STRIP method relies on a combination of conservation, phosphorylation likelihood, and binding specificity criteria and can be accessed via a web interface at ..
  11. Cheng A, Ross K, Kaldis P, Solomon M. Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases. Genes Dev. 1999;13:2946-57 pubmed
    ..Overexpression of PTC2 or PTC3 results in synthetic lethality in strains temperature-sensitive for yeast CAK1, and disruptions of PTC2 and ..
  12. Leroy C, Lee S, Vaze M, Ochsenbein F, Ochsenbien F, Guerois R, et al. PP2C phosphatases Ptc2 and Ptc3 are required for DNA checkpoint inactivation after a double-strand break. Mol Cell. 2003;11:827-35 pubmed
    ..Cells lacking the PP2C-like phosphatases Ptc2 and Ptc3 are unable to adapt to a HO-induced DSB and are also defective in recovering from a repairable DSB...