Gene Symbol: PTC1
Description: type 2C protein phosphatase PTC1
Alias: CWH47, KCS2, TPD1, type 2C protein phosphatase PTC1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. González A, Ruiz A, Casamayor A, Arino J. Normal function of the yeast TOR pathway requires the type 2C protein phosphatase Ptc1. Mol Cell Biol. 2009;29:2876-88 pubmed publisher
    Yeast ptc1 mutants are rapamycin and caffeine sensitive, suggesting a functional connection between Ptc1 and the TOR pathway that is not shared by most members of the type 2C phosphatase family...
  2. Jin Y, Taylor Eves P, Tang F, Weisman L. PTC1 is required for vacuole inheritance and promotes the association of the myosin-V vacuole-specific receptor complex. Mol Biol Cell. 2009;20:1312-23 pubmed publisher
    ..Here we show that PTC1/VAC10 controls the distribution of additional cargoes moved by a myosin-V motor...
  3. Du Y, Walker L, Novick P, Ferro Novick S. Ptc1p regulates cortical ER inheritance via Slt2p. EMBO J. 2006;25:4413-22 pubmed
    ..Our findings indicate that Ptc1p and Nbp2p regulate ER inheritance through the CWI MAPK pathway by modulating the MAPK, Slt2p. ..
  4. González A, Ruiz A, Serrano R, Arino J, Casamayor A. Transcriptional profiling of the protein phosphatase 2C family in yeast provides insights into the unique functional roles of Ptc1. J Biol Chem. 2006;281:35057-69 pubmed
    Type 2C protein phosphatases are encoded in Saccharomyces cerevisiae by several related genes (PTC1-5 and PTC7)...
  5. Li X, Du Y, Siegel S, Ferro Novick S, Novick P. Activation of the mitogen-activated protein kinase, Slt2p, at bud tips blocks a late stage of endoplasmic reticulum inheritance in Saccharomyces cerevisiae. Mol Biol Cell. 2010;21:1772-82 pubmed publisher
    ..The PI4P5-kinase, Mss4p, is an upstream activator of this pool of Slt2p. Ptc1p-dependant inactivation of Slt2p is also needed for mitochondrial inheritance; however, in this case, the relevant pool of Slt2p is not at the bud tip. ..
  6. Huang K, Symington L. Suppressors of a Saccharomyces cerevisiae pkc1 mutation identify alleles of the phosphatase gene PTC1 and of a novel gene encoding a putative basic leucine zipper protein. Genetics. 1995;141:1275-85 pubmed
    ..KCS2 is allelic with PTC1, a previously identified type 2C serine/threonine protein phosphatase...
  7. Roeder A, Hermann G, Keegan B, Thatcher S, Shaw J. Mitochondrial inheritance is delayed in Saccharomyces cerevisiae cells lacking the serine/threonine phosphatase PTC1. Mol Biol Cell. 1998;9:917-30 pubmed
    ..Cells lacking the PTC1 gene initially produce buds without a mitochondrial compartment; however, these buds later receive part of the ..
  8. Cheng A, Ross K, Kaldis P, Solomon M. Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases. Genes Dev. 1999;13:2946-57 pubmed
    ..Furthermore, PP2C-like enzymes are the predominant phosphatases toward human Cdk2 in HeLa cell extracts, indicating that the substrate specificity of PP2Cs toward CDKs is evolutionarily conserved. ..
  9. Loewen C, Young B, Tavassoli S, Levine T. Inheritance of cortical ER in yeast is required for normal septin organization. J Cell Biol. 2007;179:467-83 pubmed
    ..This perturbation leads to a delay in the transition through G2, activating the Saccharomyces wee1 kinase (Swe1) and the morphogenesis checkpoint. Thus, we identify a mechanism involved in sensing the distribution of ER. ..

More Information


  1. Ruiz A, González A, García Salcedo R, Ramos J, Arino J. Role of protein phosphatases 2C on tolerance to lithium toxicity in the yeast Saccharomyces cerevisiae. Mol Microbiol. 2006;62:263-77 pubmed
    ..of PTC3 (similarly to that of PTC2, PTC4 or PTC5) does not confer a lithium-sensitive phenotype, but mutation of PTC1 does...
  2. Rosonina E, Willis I, Manley J. Sub1 functions in osmoregulation and in transcription by both RNA polymerases II and III. Mol Cell Biol. 2009;29:2308-21 pubmed publisher
  3. Kaeberlein M, Guarente L. Saccharomyces cerevisiae MPT5 and SSD1 function in parallel pathways to promote cell wall integrity. Genetics. 2002;160:83-95 pubmed
    ..This work also provides evidence that post-transcriptional regulation is likely to be important both for maintaining cell integrity and for promoting longevity. ..
  4. Robinson M, Van Zyl W, Phizicky E, Broach J. TPD1 of Saccharomyces cerevisiae encodes a protein phosphatase 2C-like activity implicated in tRNA splicing and cell separation. Mol Cell Biol. 1994;14:3634-45 pubmed
    The Saccharomyces cerevisiae TPD1 gene has been implicated in tRNA splicing because a tpd1-1 mutant strain accumulates unspliced precursor tRNAs at high temperatures (W. H. van Zyl, N. Wills, and J. R. Broach, Genetics 123:55-68, 1989)...
  5. Tahirovic S, Schorr M, Mayinger P. Regulation of intracellular phosphatidylinositol-4-phosphate by the Sac1 lipid phosphatase. Traffic. 2005;6:116-30 pubmed
    ..We conclude that Sac1p functions in confining PtdIns(4)P-dependent processes to specific intracellular membranes. ..
  6. Shitamukai A, Hirata D, Sonobe S, Miyakawa T. Evidence for antagonistic regulation of cell growth by the calcineurin and high osmolarity glycerol pathways in Saccharomyces cerevisiae. J Biol Chem. 2004;279:3651-61 pubmed
  7. Ohkuni K, Okuda A, Kikuchi A. Yeast Nap1-binding protein Nbp2p is required for mitotic growth at high temperatures and for cell wall integrity. Genetics. 2003;165:517-29 pubmed
    ..Genetic analyses suggested that Ptc1, which interacts with Nbp2 by the two-hybrid system, acts downstream of Nbp2 and that cells lacking the function of ..
  8. Warmka J, Hanneman J, Lee J, Amin D, Ota I. Ptc1, a type 2C Ser/Thr phosphatase, inactivates the HOG pathway by dephosphorylating the mitogen-activated protein kinase Hog1. Mol Cell Biol. 2001;21:51-60 pubmed
    ..Three type 2C Ser/Thr phosphatases (PTCs), Ptc1, Ptc2, and Ptc3, have been isolated as negative regulators of this pathway...
  9. Guo J, Polymenis M. Dcr2 targets Ire1 and downregulates the unfolded protein response in Saccharomyces cerevisiae. EMBO Rep. 2006;7:1124-7 pubmed
    ..Our results are consistent with de-phosphorylation of Ire1 being a mechanism for antagonizing UPR signalling. ..
  10. González A, Casado C, Arino J, Casamayor A. Ptc6 is required for proper rapamycin-induced down-regulation of the genes coding for ribosomal and rRNA processing proteins in S. cerevisiae. PLoS ONE. 2013;8:e64470 pubmed publisher
    Ptc6 is one of the seven components (Ptc1-Ptc7) of the protein phosphatase 2C family in the yeast Saccharomyces cerevisiae. In contrast to other type 2C phosphatases, the cellular role of this isoform is poorly understood...
  11. Malleshaiah M, Shahrezaei V, Swain P, Michnick S. The scaffold protein Ste5 directly controls a switch-like mating decision in yeast. Nature. 2010;465:101-5 pubmed publisher
    ..Here we show that the switching mechanism arises from competition between the MAPK Fus3 and a phosphatase Ptc1 for control of the phosphorylation state of four sites on the scaffold protein Ste5...
  12. Swayne T, Zhou C, Boldogh I, Charalel J, MCFALINE FIGUEROA J, Thoms S, et al. Role for cER and Mmr1p in anchorage of mitochondria at sites of polarized surface growth in budding yeast. Curr Biol. 2011;21:1994-9 pubmed publisher
    ..Deletion of the phosphatase PTC1 results in increased Mmr1p phosphorylation, mislocalization of Mmr1p, defects in association of Mmr1p with ..
  13. Sharmin D, Sasano Y, Sugiyama M, Harashima S. Type 2C protein phosphatase Ptc6 participates in activation of the Slt2-mediated cell wall integrity pathway in Saccharomyces cerevisiae. J Biosci Bioeng. 2015;119:392-8 pubmed publisher
    ..Saccharomyces cerevisiae genome encodes 40 PPases, including seven members of the protein phosphatase 2C subfamily (PTC1 to PTC7). In contrast to other PPases, the cellular roles of PTCs have not been investigated in detail...
  14. He X, van Waardenburg R, Babaoglu K, Price A, Nitiss K, Nitiss J, et al. Mutation of a conserved active site residue converts tyrosyl-DNA phosphodiesterase I into a DNA topoisomerase I-dependent poison. J Mol Biol. 2007;372:1070-81 pubmed
    ..However, the distinct pattern of mutant Tdp1 activity evident in yeast cells, suggests a more severe defect in Tdp1H(432)N-catalyzed resolution of 3' phospho-adducts. ..
  15. Sharmin D, Sasano Y, Sugiyama M, Harashima S. Effects of deletion of different PP2C protein phosphatase genes on stress responses in Saccharomyces cerevisiae. Yeast. 2014;31:393-409 pubmed publisher
    ..Saccharomyces cerevisiae has 40 PPases, including seven protein phosphatase 2C (PP2C PPase) genes (PTC1-PTC7). However, their precise functions remain poorly understood...
  16. Ruiz A, Xu X, Carlson M. Ptc1 protein phosphatase 2C contributes to glucose regulation of SNF1/AMP-activated protein kinase (AMPK) in Saccharomyces cerevisiae. J Biol Chem. 2013;288:31052-8 pubmed publisher
    ..We here present evidence that Ptc1 protein phosphatase 2C also has a role in dephosphorylation of Snf1 Thr-210 in vivo...
  17. Ketela T, Brown J, Stewart R, Bussey H. Yeast Skn7p activity is modulated by the Sln1p-Ypd1p osmosensor and contributes to regulation of the HOG pathway. Mol Gen Genet. 1998;259:372-8 pubmed
    ..We also observed that loss of PTC1 (a protein serine/threonine phosphatase implicated in negative control of the HOG pathway) in a skn7delta ..
  18. Li X, Ferro Novick S, Novick P. Different polarisome components play distinct roles in Slt2p-regulated cortical ER inheritance in Saccharomyces cerevisiae. Mol Biol Cell. 2013;24:3145-54 pubmed publisher
    ..In ptc1? cells, ER tubules migrate from the mother cell and contact the bud tip, yet fail to spread around the bud cortex...
  19. Tatjer L, Sacristán Reviriego A, Casado C, González A, Rodríguez Porrata B, Palacios L, et al. Wide-Ranging Effects of the Yeast Ptc1 Protein Phosphatase Acting Through the MAPK Kinase Mkk1. Genetics. 2016;202:141-56 pubmed publisher
    The Saccharomyces cerevisiae type 2C protein phosphatase Ptc1 is required for a wide variety of cellular functions, although only a few cellular targets have been identified...
  20. Kelly M, Brosnan L, Jauert P, Dunham M, Kirkpatrick D. Multiple pathways regulate minisatellite stability during stationary phase in yeast. G3 (Bethesda). 2012;2:1185-95 pubmed publisher
    ..This result indicates that the genetic control of stationary phase minisatellite stability is dependent on the sequence composition of the minisatellite itself. ..
  21. Stanger K, Gorelik M, Davidson A. Yeast adaptor protein, Nbp2p, is conserved regulator of fungal Ptc1p phosphatases and is involved in multiple signaling pathways. J Biol Chem. 2012;287:22133-41 pubmed publisher
    ..Our results also clearly imply a function for the Nbp2p-Ptc1p complex other cellular processes. ..
  22. Mapes J, Ota I. Nbp2 targets the Ptc1-type 2C Ser/Thr phosphatase to the HOG MAPK pathway. EMBO J. 2004;23:302-11 pubmed
    ..The Ptc1-type 2C Ser/Thr phosphatase (PP2C) inactivates Hog1 by dephosphorylating phospho-Thr, while the Ptp2 and Ptp3 ..
  23. Bensen E, Costaguta G, Payne G. Synthetic genetic interactions with temperature-sensitive clathrin in Saccharomyces cerevisiae. Roles for synaptojanin-like Inp53p and dynamin-related Vps1p in clathrin-dependent protein sorting at the trans-Golgi network. Genetics. 2000;154:83-97 pubmed
    ..One mutant with unaltered protein sorting at the TGN contains a mutation in PTC1, a type 2c serine/threonine phosphatase with widespread influences...
  24. Mattison C, Ota I. Two protein tyrosine phosphatases, Ptp2 and Ptp3, modulate the subcellular localization of the Hog1 MAP kinase in yeast. Genes Dev. 2000;14:1229-35 pubmed
    ..In support of this result PTP2 overexpression sequestered Hog1 in the nucleus while PTP3 overexpression restricted Hog1 to the cytoplasm. Thus, Ptp2 and Ptp3 regulate Hog1 localization by binding Hog1. ..
  25. Neller J, Dünkler A, Rösler R, Johnsson N. A protein complex containing Epo1p anchors the cortical endoplasmic reticulum to the yeast bud tip. J Cell Biol. 2015;208:71-87 pubmed publisher
    ..This analysis therefore identifies Epo1p as a novel and important component of the polarisome that promotes cER tethering at sites of polarized growth. ..
  26. Maeda T, Wurgler Murphy S, Saito H. A two-component system that regulates an osmosensing MAP kinase cascade in yeast. Nature. 1994;369:242-5 pubmed
    ..Here we describe a two-component system in Saccharomyces cerevisiae that regulates an osmosensing MAP kinase cascade. ..
  27. Maeda T, Tsai A, Saito H. Mutations in a protein tyrosine phosphatase gene (PTP2) and a protein serine/threonine phosphatase gene (PTC1) cause a synthetic growth defect in Saccharomyces cerevisiae. Mol Cell Biol. 1993;13:5408-17 pubmed
    ..Cloning and sequence determination of one of these gene, PTC1, indicated that it encodes a homolog of the mammalian protein serine/threonine phosphatase 2C (PP2C)...
  28. Protchenko O, Rodriguez Suarez R, Androphy R, Bussey H, Philpott C. A screen for genes of heme uptake identifies the FLC family required for import of FAD into the endoplasmic reticulum. J Biol Chem. 2006;281:21445-57 pubmed
    ..We propose that the FLC genes are required for import of FAD into the lumen of the endoplasmic reticulum, where it is required for disulfide bond formation. ..