Gene Symbol: PTA1
Description: RNA-processing protein PTA1
Alias: FUN39, RNA-processing protein PTA1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Nedea E, He X, Kim M, Pootoolal J, Zhong G, Canadien V, et al. Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem. 2003;278:33000-10 pubmed
    ..phosphatase (Glc7), Ssu72 (a TFIIB and RNA polymerase II-associated factor), Ref2, and Syc1 are associated with the Pta1 subunit of CPF...
  2. Nedea E, Nalbant D, Xia D, Theoharis N, Suter B, Richardson C, et al. The Glc7 phosphatase subunit of the cleavage and polyadenylation factor is essential for transcription termination on snoRNA genes. Mol Cell. 2008;29:577-87 pubmed publisher
    ..Swd2 is also a subunit of the Set1c histone H3K4 methyltransferase complex and is required for its stability and optimal methyltransferase activity. ..
  3. Zhao J, Kessler M, Helmling S, O Connor J, Moore C. Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA precursor. Mol Cell Biol. 1999;19:7733-40 pubmed
    ..In this report, we show by protein sequencing and immunoreactivity that the fourth subunit of CF II is Pta1, an essential 90-kDa protein originally implicated in tRNA splicing...
  4. Kim M, Krogan N, Vasiljeva L, Rando O, Nedea E, Greenblatt J, et al. The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II. Nature. 2004;432:517-22 pubmed
    ..These findings support a model in which poly(A) site cleavage and subsequent degradation of the 3'-downstream RNA by Rat1 trigger transcription termination. ..
  5. Ghazy M, He X, Singh B, Hampsey M, Moore C. The essential N terminus of the Pta1 scaffold protein is required for snoRNA transcription termination and Ssu72 function but is dispensable for pre-mRNA 3'-end processing. Mol Cell Biol. 2009;29:2296-307 pubmed publisher
    Saccharomyces cerevisiae Pta1 is a component of the cleavage/polyadenylation factor (CPF) 3'-end processing complex and functions in pre-mRNA cleavage, poly(A) addition, and transcription termination...
  6. Zhelkovsky A, Tacahashi Y, Nasser T, He X, Sterzer U, Jensen T, et al. The role of the Brr5/Ysh1 C-terminal domain and its homolog Syc1 in mRNA 3'-end processing in Saccharomyces cerevisiae. RNA. 2006;12:435-45 pubmed
    ..Our findings suggest that Syc1, by mimicking the essential Brr5 C-terminus, serves as a negative regulator of mRNA 3'-end formation. ..
  7. He X, Khan A, Cheng H, Pappas D, Hampsey M, Moore C. Functional interactions between the transcription and mRNA 3' end processing machineries mediated by Ssu72 and Sub1. Genes Dev. 2003;17:1030-42 pubmed
    ..Ssu72 interacts physically and genetically with the Pta1 subunit of CPF. Overexpression of PTA1 causes synthetic lethality in an ssu72-3 mutant...
  8. Kyburz A, Sadowski M, Dichtl B, Keller W. The role of the yeast cleavage and polyadenylation factor subunit Ydh1p/Cft2p in pre-mRNA 3'-end formation. Nucleic Acids Res. 2003;31:3936-45 pubmed
  9. Ohnacker M, Barabino S, Preker P, Keller W. The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex. EMBO J. 2000;19:37-47 pubmed
    ..These results show that Pfs2p plays an essential role in 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex. ..

More Information


  1. He X, Moore C. Regulation of yeast mRNA 3' end processing by phosphorylation. Mol Cell. 2005;19:619-29 pubmed
    ..Here, we report that depletion of Glc7 causes shortened poly(A) tails in vivo and accumulation of phosphorylated Pta1, a CPF subunit...
  2. Dheur S, Nykamp K, Viphakone N, Swanson M, Minvielle Sebastia L. Yeast mRNA Poly(A) tail length control can be reconstituted in vitro in the absence of Pab1p-dependent Poly(A) nuclease activity. J Biol Chem. 2005;280:24532-8 pubmed
    ..Based on these findings, we propose that Nab2p is necessary and sufficient to regulate poly(A) tail length during de novo synthesis in yeast. ..
  3. Ghazy M, Gordon J, Lee S, Singh B, Bohm A, Hampsey M, et al. The interaction of Pcf11 and Clp1 is needed for mRNA 3'-end formation and is modulated by amino acids in the ATP-binding site. Nucleic Acids Res. 2012;40:1214-25 pubmed publisher
    ..These results define Clp1 as a bridge between CF IA and CPF and indicate that the Clp1-Pcf11 interaction is modulated by amino acids in the conserved ATP-binding site of Clp1. ..
  4. Seoane S, Lamas Maceiras M, Rodríguez Torres A, Freire Picos M. Involvement of Pta1, Pcf11 and a KlCYC1 AU-rich element in alternative RNA 3'-end processing selection in yeast. FEBS Lett. 2009;583:2843-8 pubmed publisher
    This work reports the involvement of yeast RNA processing factors Pta1 and Pcf11 in alternative 3'-end RNA processing. The pta1-1 and pcf11-2 mutations changed the predominance of KlCYC1 1.14 and 1.5 kb transcript isoforms...
  5. Schreieck A, Easter A, Etzold S, Wiederhold K, Lidschreiber M, Cramer P, et al. RNA polymerase II termination involves C-terminal-domain tyrosine dephosphorylation by CPF subunit Glc7. Nat Struct Mol Biol. 2014;21:175-179 pubmed publisher
    ..These results show that transcription termination involves Tyr1 dephosphorylation of the CTD and indicate that pre-mRNA processing by CPF and transcription termination are coupled via Glc7-dependent Pol II-Tyr1 dephosphorylation. ..
  6. Ezeokonkwo C, Zhelkovsky A, Lee R, Bohm A, Moore C. A flexible linker region in Fip1 is needed for efficient mRNA polyadenylation. RNA. 2011;17:652-64 pubmed publisher
    ..Our results indicate that the Fip1 linker, through its flexibility and protein/protein interactions, allows Pap1 to reach the 3' end of the cleaved RNA and efficiently initiate poly(A) addition. ..
  7. Zhang D, Mosley A, Ramisetty S, Rodríguez Molina J, Washburn M, Ansari A. Ssu72 phosphatase-dependent erasure of phospho-Ser7 marks on the RNA polymerase II C-terminal domain is essential for viability and transcription termination. J Biol Chem. 2012;287:8541-51 pubmed publisher
    ..An inability to remove these marks prevents Pol II from terminating efficiently and will likely impede subsequent assembly into the pre-initiation complex. ..
  8. Preker P, Ohnacker M, Minvielle Sebastia L, Keller W. A multisubunit 3' end processing factor from yeast containing poly(A) polymerase and homologues of the subunits of mammalian cleavage and polyadenylation specificity factor. EMBO J. 1997;16:4727-37 pubmed
    ..As expected for a PF I subunit, pta1-1 mutant extracts are deficient for polyadenylation in vitro...
  9. Roguev A, Schaft D, Shevchenko A, Pijnappel W, Wilm M, Aasland R, et al. The Saccharomyces cerevisiae Set1 complex includes an Ash2 homologue and methylates histone 3 lysine 4. EMBO J. 2001;20:7137-48 pubmed
    ..We propose that eukaryotic Set1Cs are H3 lysine 4 methyltransferases and are related to trxG action through association with Ash2 homologues. ..
  10. Vo L, Minet M, Schmitter J, Lacroute F, Wyers F. Mpe1, a zinc knuckle protein, is an essential component of yeast cleavage and polyadenylation factor required for the cleavage and polyadenylation of mRNA. Mol Cell Biol. 2001;21:8346-56 pubmed
    ..These results show that Mpe1p plays a crucial role in 3' end formation probably by promoting the specific link between the CFI/CPF complex and pre-mRNA. ..
  11. Lee S, Moore C. Efficient mRNA polyadenylation requires a ubiquitin-like domain, a zinc knuckle, and a RING finger domain, all contained in the Mpe1 protein. Mol Cell Biol. 2014;34:3955-67 pubmed publisher
    ..Furthermore, an inhibitor of ubiquitin-mediated interactions blocks cleavage, demonstrating for the first time a direct role for ubiquitination in mRNA 3'-end processing. ..
  12. Skaar D, Greenleaf A. The RNA polymerase II CTD kinase CTDK-I affects pre-mRNA 3' cleavage/polyadenylation through the processing component Pti1p. Mol Cell. 2002;10:1429-39 pubmed
    ..Therefore, one important function of the CTDK-I-generated phospho-CTD appears to be the coupling of transcription to 3' processing of pre-mRNAs by a Pti1p-containing complex. ..
  13. Krishnamurthy S, Ghazy M, Moore C, Hampsey M. Functional interaction of the Ess1 prolyl isomerase with components of the RNA polymerase II initiation and termination machineries. Mol Cell Biol. 2009;29:2925-34 pubmed publisher
    ..functionally interacts with the transcription initiation factor TFIIB and with the Ssu72 CTD phosphatase and Pta1 components of the CPF 3'-end processing complex...
  14. Zhao J, Kessler M, Moore C. Cleavage factor II of Saccharomyces cerevisiae contains homologues to subunits of the mammalian Cleavage/ polyadenylation specificity factor and exhibits sequence-specific, ATP-dependent interaction with precursor RNA. J Biol Chem. 1997;272:10831-8 pubmed
    ..These results provide additional evidence that certain features of the molecular mechanism of mRNA 3'-end formation are conserved between yeast and mammals, but also highlight unexpected differences. ..
  15. Rodriguez C, Cho E, Keogh M, Moore C, Greenleaf A, Buratowski S. Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II. Mol Cell Biol. 2000;20:104-12 pubmed
    ..Interestingly, levels of the polyadenylation factor Pta1 are also reduced in kin28 mutants, while several other polyadenylation factors remain stable...
  16. O Connor J, Peebles C. PTA1, an essential gene of Saccharomyces cerevisiae affecting pre-tRNA processing. Mol Cell Biol. 1992;12:3843-56 pubmed
    We have identified an essential Saccharomyces cerevisiae gene, PTA1, that affects pre-tRNA processing...
  17. Dichtl B, Blank D, Ohnacker M, Friedlein A, Roeder D, Langen H, et al. A role for SSU72 in balancing RNA polymerase II transcription elongation and termination. Mol Cell. 2002;10:1139-50 pubmed
    ..The sum of our analyses suggests a negative influence of Ssu72p on RNAP II during transcription that affects the commitment to either elongation or termination. ..
  18. Ezeokonkwo C, Ghazy M, Zhelkovsky A, Yeh P, Moore C. Novel interactions at the essential N-terminus of poly(A) polymerase that could regulate poly(A) addition in Saccharomyces cerevisiae. FEBS Lett. 2012;586:1173-8 pubmed publisher
    ..We have now identified proteins that interact specifically with this region. Cft1 and Pta1 are subunits of the cleavage/polyadenylation factor, in which Pap1 resides, and Nab6 and Sub1 are nucleic-acid ..