PRP8

Summary

Gene Symbol: PRP8
Description: U4/U6-U5 snRNP complex subunit PRP8
Alias: DBF3, DNA39, RNA8, SLT21, USA2, U4/U6-U5 snRNP complex subunit PRP8
Species: Saccharomyces cerevisiae S288c
Products:     PRP8

Top Publications

  1. Bellare P, Kutach A, Rines A, Guthrie C, Sontheimer E. Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p. RNA. 2006;12:292-302 pubmed
    ..Our results define a new UBD and suggest functional links between ubiquitin and the pre-mRNA splicing machinery. ..
  2. Kaplan Y, Kupiec M. A role for the yeast cell cycle/splicing factor Cdc40 in the G1/S transition. Curr Genet. 2007;51:123-40 pubmed
    ..Finally, we discuss possible mechanisms of suppression by the cDNAs that imply cell cycle regulation by apparently unrelated processes, such as splicing, translation initiation and glycolysis. ..
  3. Liu L, Query C, Konarska M. Opposing classes of prp8 alleles modulate the transition between the catalytic steps of pre-mRNA splicing. Nat Struct Mol Biol. 2007;14:519-26 pubmed
    ..Here, we identify and characterize a class of prp8 mutants that suppress first-step splicing defects and oppose the action of the previously described prp8 ..
  4. Maeder C, Kutach A, Guthrie C. ATP-dependent unwinding of U4/U6 snRNAs by the Brr2 helicase requires the C terminus of Prp8. Nat Struct Mol Biol. 2009;16:42-8 pubmed publisher
    ..dissociation of U4/U6 snRNAs in vitro is activated by a fragment from the C terminus of the U5 snRNP protein Prp8. In contrast to its helicase-stimulating activity, this fragment inhibits Brr2 U4/U6-dependent ATPase activity...
  5. Boon K, Grainger R, Ehsani P, Barrass J, Auchynnikava T, Inglehearn C, et al. prp8 mutations that cause human retinitis pigmentosa lead to a U5 snRNP maturation defect in yeast. Nat Struct Mol Biol. 2007;14:1077-83 pubmed
    b>Prp8 protein (Prp8p) is a highly conserved pre-mRNA splicing factor and a component of spliceosomal U5 small nuclear ribonucleoproteins (snRNPs)...
  6. Bouveret E, Rigaut G, Shevchenko A, Wilm M, Seraphin B. A Sm-like protein complex that participates in mRNA degradation. EMBO J. 2000;19:1661-71 pubmed
    ..These results indicate the involvement of a new conserved Sm-like protein complex and a new factor, Pat1p, in mRNA degradation and suggest a physical connection between decapping and exonuclease trimming. ..
  7. Galej W, Oubridge C, Newman A, Nagai K. Crystal structure of Prp8 reveals active site cavity of the spliceosome. Nature. 2013;493:638-43 pubmed publisher
    ..b>Prp8, a component of the U5 small nuclear ribonucleoprotein particle, crosslinks extensively with this RNA catalytic ..
  8. Boon K, Norman C, Grainger R, Newman A, Beggs J. Prp8p dissection reveals domain structure and protein interaction sites. RNA. 2006;12:198-205 pubmed
    ..When used as a genetic screen to dissect the budding yeast PRP8 gene, this showed that Prp8 protein could be dissected into three distinct pairs of functional polypeptides...
  9. Xu D, Field D, Tang S, Moris A, Bobechko B, Friesen J. Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing. Mol Cell Biol. 1998;18:2055-66 pubmed
    ..splicing genes: slt15, previously identified as prp17 (slt15/prp17-100), slt16/smd3-1, slt17/slu7-100, and slt21/prp8-21...

More Information

Publications68

  1. Zhang L, Xu T, Maeder C, Bud L, Shanks J, Nix J, et al. Structural evidence for consecutive Hel308-like modules in the spliceosomal ATPase Brr2. Nat Struct Mol Biol. 2009;16:731-9 pubmed publisher
    ..We demonstrate that Hel308-II interacts with Prp8 and Snu114 in vitro and in vivo...
  2. Brenner T, Guthrie C. Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain. RNA. 2006;12:862-71 pubmed
    ..identified allele-specific genetic interactions between SNU114 and genes encoding three other U5 snRNP components, Prp8 and two RNA-dependent ATPases, Prp28 and Brr2, required for destabilization of U1 and U4 snRNPs prior to catalysis...
  3. Kuhn A, Brow D. Suppressors of a cold-sensitive mutation in yeast U4 RNA define five domains in the splicing factor Prp8 that influence spliceosome activation. Genetics. 2000;155:1667-82 pubmed
    The highly conserved splicing factor Prp8 has been implicated in multiple stages of the splicing reaction...
  4. Brenner T, Guthrie C. Genetic analysis reveals a role for the C terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activation. Genetics. 2005;170:1063-80 pubmed
    ..The growth defects caused by most alleles were synthetically exacerbated by mutations in PRP8, a U5 snRNP protein that physically interacts with Snu114, as well as in genes involved in snRNP biogenesis, ..
  5. Kuhn A, Reichl E, Brow D. Distinct domains of splicing factor Prp8 mediate different aspects of spliceosome activation. Proc Natl Acad Sci U S A. 2002;99:9145-9 pubmed
    b>Prp8 is the largest and most highly conserved protein in the spliceosome yet its mechanism of function is poorly understood...
  6. Weber G, Cristão V, de L Alves F, Santos K, Holton N, Rappsilber J, et al. Mechanism for Aar2p function as a U5 snRNP assembly factor. Genes Dev. 2011;25:1601-12 pubmed publisher
    ..The purpose of this regulation may be to safeguard against nonspecific RNA binding to Prp8p and/or premature activation of Brr2p activity. ..
  7. Dix I, Russell C, O Keefe R, Newman A, Beggs J. Protein-RNA interactions in the U5 snRNP of Saccharomyces cerevisiae. RNA. 1998;4:1675-86 pubmed
    ..This and the close proximity of the spliceosomal translocase, Snu114p, to U5 loop 1 and Prp8p support and extend the proposal that Snu114p mimics U5 loop 1 during a translocation event in the spliceosome. ..
  8. Häcker I, Sander B, Golas M, Wolf E, Karagöz E, Kastner B, et al. Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy. Nat Struct Mol Biol. 2008;15:1206-12 pubmed publisher
    ..activation that requires several components of the U5 portion of the tri-snRNP, including the RNA helicase Brr2, Prp8 and the GTPase Snu114. Here we report the EM projection structure of the Saccharomyces cerevisiae tri-snRNP...
  9. Pena V, Liu S, Bujnicki J, Luhrmann R, Wahl M. Structure of a multipartite protein-protein interaction domain in splicing factor prp8 and its link to retinitis pigmentosa. Mol Cell. 2007;25:615-24 pubmed
    Protein Prp8 interacts with several other spliceosomal proteins, snRNAs, and the pre-mRNA and thereby organizes the active site(s) of the spliceosome...
  10. Query C, Konarska M. Suppression of multiple substrate mutations by spliceosomal prp8 alleles suggests functional correlations with ribosomal ambiguity mutants. Mol Cell. 2004;14:343-54 pubmed
    ..A genetic screen for suppressors of BS A-to-G mutants, which stall between the two steps, identified Prp8, the highly conserved spliceosomal factor...
  11. Pena V, Rozov A, Fabrizio P, Luhrmann R, Wahl M. Structure and function of an RNase H domain at the heart of the spliceosome. EMBO J. 2008;27:2929-40 pubmed publisher
    ..Protein Prp8 is an active site component but the molecular mechanisms, by which it might facilitate splicing catalysis, are ..
  12. Yang K, Zhang L, Xu T, Heroux A, Zhao R. Crystal structure of the beta-finger domain of Prp8 reveals analogy to ribosomal proteins. Proc Natl Acad Sci U S A. 2008;105:13817-22 pubmed publisher
    b>Prp8 stands out among hundreds of splicing factors as a key regulator of spliceosome activation and a potential cofactor of the splicing reaction...
  13. Ritchie D, Schellenberg M, Gesner E, Raithatha S, Stuart D, Macmillan A. Structural elucidation of a PRP8 core domain from the heart of the spliceosome. Nat Struct Mol Biol. 2008;15:1199-205 pubmed publisher
    ..One of these proteins, PRP8, has been shown to interact directly with the splice sites and branch region of precursor-mRNAs (pre-mRNAs) and ..
  14. Gottschalk A, Kastner B, Luhrmann R, Fabrizio P. The yeast U5 snRNP coisolated with the U1 snRNP has an unexpected protein composition and includes the splicing factor Aar2p. RNA. 2001;7:1554-65 pubmed
    ..Thus, the basic structural scaffold of the Aar2-U5 snRNP seems to be essentially determined by Prp8p, Snu114p, and the Sm proteins. ..
  15. Garrey S, Katolik A, Prekeris M, Li X, York K, Bernards S, et al. A homolog of lariat-debranching enzyme modulates turnover of branched RNA. RNA. 2014;20:1337-48 pubmed publisher
    ..Drn1 enhances Dbr1-mediated turnover of lariat-intermediates and lariat-intron products, indicating that branched RNA turnover is regulated at multiple steps during splicing...
  16. Ledoux S, Guthrie C. Retinitis Pigmentosa Mutations in Bad Response to Refrigeration 2 (Brr2) Impair ATPase and Helicase Activity. J Biol Chem. 2016;291:11954-65 pubmed publisher
    ..The globular C-terminal Jab1/Mpn1-like domain of Prp8 increases the ability of ?247-Brr2 to bind the U4/U6 snRNA duplex at high pH and increases ?247-Brr2's RNA-..
  17. Li X, Zhang W, Xu T, Ramsey J, Zhang L, Hill R, et al. Comprehensive in vivo RNA-binding site analyses reveal a role of Prp8 in spliceosomal assembly. Nucleic Acids Res. 2013;41:3805-18 pubmed publisher
    b>Prp8 stands out among hundreds of splicing factors as a protein that is intimately involved in spliceosomal activation and the catalytic reaction...
  18. Mayerle M, Raghavan M, Ledoux S, Price A, Stepankiw N, Hadjivassiliou H, et al. Structural toggle in the RNaseH domain of Prp8 helps balance splicing fidelity and catalytic efficiency. Proc Natl Acad Sci U S A. 2017;114:4739-4744 pubmed publisher
    ..b>Prp8 has long been considered the "master regulator" of the spliceosome, the molecular machine that executes pre-mRNA ..
  19. Masciadri B, Areces L, Carpinelli P, Foiani M, Draetta G, Fiore F. Characterization of the BUD31 gene of Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2004;320:1342-50 pubmed
    ..We propose that the observed phenotypes for bud31-null strain could be the result of defective splicing and indicate a first functional role for Bud3lp and its homologs. ..
  20. Jamieson D, Rahe B, Pringle J, Beggs J. A suppressor of a yeast splicing mutation (prp8-1) encodes a putative ATP-dependent RNA helicase. Nature. 1991;349:715-7 pubmed
    ..Several PRP genes have now been cloned and their protein products characterized. The PRP8 protein is a component of the U5 snRNP and associates with the U4/U6 snRNAs/snRNP to form a multi-snRNP particle ..
  21. Chanarat S, Seizl M, Strasser K. The Prp19 complex is a novel transcription elongation factor required for TREX occupancy at transcribed genes. Genes Dev. 2011;25:1147-58 pubmed publisher
  22. Pandit S, Lynn B, Rymond B. Inhibition of a spliceosome turnover pathway suppresses splicing defects. Proc Natl Acad Sci U S A. 2006;103:13700-5 pubmed
    ..These and related genetic data support the existence of a Spp382p-dependent turnover pathway acting on defective spliceosomes. ..
  23. Schellenberg M, Wu T, Ritchie D, Fica S, Staley J, Atta K, et al. A conformational switch in PRP8 mediates metal ion coordination that promotes pre-mRNA exon ligation. Nat Struct Mol Biol. 2013;20:728-34 pubmed publisher
    ..A wealth of evidence places the protein PRP8 at the heart of the spliceosome through assembly and catalysis...
  24. Bialkowska A, Kurlandzka A. Proteins interacting with Lin 1p, a putative link between chromosome segregation, mRNA splicing and DNA replication in Saccharomyces cerevisiae. Yeast. 2002;19:1323-33 pubmed
    ..The second round of two-hybrid assay with Lin1p as the bait resulted in the identification of six proteins: Prp8, Slx5, Siz2, Wss1, Rfc1 and YIL149w...
  25. Query C, Konarska M. CEF1/CDC5 alleles modulate transitions between catalytic conformations of the spliceosome. RNA. 2012;18:1001-13 pubmed publisher
    ..A prior genetic screen for suppressors of an intron mutant that stalls between the two steps yielded both prp8 and non-prp8 alleles that suppressed second-step splicing defects...
  26. Absmeier E, Wollenhaupt J, Mozaffari Jovin S, Becke C, Lee C, Preussner M, et al. The large N-terminal region of the Brr2 RNA helicase guides productive spliceosome activation. Genes Dev. 2015;29:2576-87 pubmed publisher
    ..Here, we show by crystal structure analysis of full-length Brr2 in complex with a regulatory Jab1/MPN domain of the Prp8 protein and by cross-linking/mass spectrometry of isolated Brr2 that the Brr2 N-terminal region encompasses two ..
  27. Hackmann A, Wu H, Schneider U, Meyer K, Jung K, Krebber H. Quality control of spliced mRNAs requires the shuttling SR proteins Gbp2 and Hrb1. Nat Commun. 2014;5:3123 pubmed publisher
    ..Altogether, these data identify a role for shuttling SR proteins in mRNA surveillance and nuclear mRNA quality control. ..
  28. Pandit S, Paul S, Zhang L, Chen M, Durbin N, Harrison S, et al. Spp382p interacts with multiple yeast splicing factors, including possible regulators of Prp43 DExD/H-Box protein function. Genetics. 2009;183:195-206 pubmed publisher
    ..These and related data establish the value of this dosage interference assay for finding genes that alter cellular splicing and define Sqs1p and Cwc23p as prospective modulators of Spp382p-stimuated Prp43p function. ..
  29. Dagher S, Fu X. Evidence for a role of Sky1p-mediated phosphorylation in 3' splice site recognition involving both Prp8 and Prp17/Slu4. RNA. 2001;7:1284-97 pubmed
    ..Here we report that SKY1, a SRPK family member in Saccharomyces cerevisiae, genetically interacts with PRP8 and PRP17/SLU4, both of which are involved in splice site selection during pre-mRNA splicing...
  30. Hertveldt K, Dechassa M, Robben J, Volckaert G. Identification of Gal80p-interacting proteins by Saccharomyces cerevisiae whole genome phage display. Gene. 2003;307:141-9 pubmed
    ..Interactions were confirmed by ELISA. These results demonstrate the accessibility of the S. cerevisiae genome to display technology for protein-protein interaction screening. ..
  31. Görnemann J, Kotovic K, Hujer K, Neugebauer K. Cotranscriptional spliceosome assembly occurs in a stepwise fashion and requires the cap binding complex. Mol Cell. 2005;19:53-63 pubmed
    ..Thus, the demonstration of an essential link between CBC and spliceosome assembly in vivo indicates that 5' end capping couples pre-mRNA splicing to transcription. ..
  32. Schneider S, Campodonico E, Schwer B. Motifs IV and V in the DEAH box splicing factor Prp22 are important for RNA unwinding, and helicase-defective Prp22 mutants are suppressed by Prp8. J Biol Chem. 2004;279:8617-26 pubmed
    ..We identified one of the suppressors as a missense mutation of PRP8 (R1753K), a protein component of the U5 small nuclear ribonucleoprotein...
  33. Mayerle M, Guthrie C. Prp8 retinitis pigmentosa mutants cause defects in the transition between the catalytic steps of splicing. RNA. 2016;22:793-809 pubmed publisher
    ..b>Prp8 and Snu114, an EF2-like GTPase, regulate the activity of the Brr2 helicase, promoting RNA unwinding by Brr2 at ..
  34. Liang W, Cheng S. A novel mechanism for Prp5 function in prespliceosome formation and proofreading the branch site sequence. Genes Dev. 2015;29:81-93 pubmed publisher
    ..Mutations impairing U2-branch site base-pairing retard Prp5 release and impede tri-snRNP association. Prp5 mutations that destabilize the Prp5-U2 interaction suppress branch site mutations by allowing progression of the pathway. ..
  35. Weber G, Cristão V, Santos K, Jovin S, Heroven A, Holton N, et al. Structural basis for dual roles of Aar2p in U5 snRNP assembly. Genes Dev. 2013;27:525-40 pubmed publisher
    ..nuclear ribonucleoprotein particle (snRNP) is assembled via a cytoplasmic precursor that contains the U5-specific Prp8 protein but lacks the U5-specific Brr2 helicase...
  36. Gottschalk A, Tang J, Puig O, Salgado J, Neubauer G, Colot H, et al. A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins. RNA. 1998;4:374-93 pubmed
    ..Finally, we show that Nam8p/Mud15p contributes to the stability of U1 snRNP. ..
  37. Taoka M, Yamauchi Y, Nobe Y, Masaki S, Nakayama H, Ishikawa H, et al. An analytical platform for mass spectrometry-based identification and chemical analysis of RNA in ribonucleoprotein complexes. Nucleic Acids Res. 2009;37:e140 pubmed publisher
  38. Shea J, Toyn J, Johnston L. The budding yeast U5 snRNP Prp8 is a highly conserved protein which links RNA splicing with cell cycle progression. Nucleic Acids Res. 1994;22:5555-64 pubmed
    ..The nucleotide sequence of DBF3 turned out to be identical to the yeast gene PRP8, which encodes a U5 snRNP required for pre-mRNA splicing...
  39. Maddock J, Weidenhammer E, Adams C, Lunz R, Woolford J. Extragenic suppressors of Saccharomyces cerevisiae prp4 mutations identify a negative regulator of PRP genes. Genetics. 1994;136:833-47 pubmed
    ..SPP41 was cloned and sequenced and found to be essential. spp43 is allelic to the previously identified suppressor srn1, which encodes a negative regulator of gene expression. ..
  40. Price A, Görnemann J, Guthrie C, Brow D. An unanticipated early function of DEAD-box ATPase Prp28 during commitment to splicing is modulated by U5 snRNP protein Prp8. RNA. 2014;20:46-60 pubmed publisher
    ..Because Prp8, an integral U5 snRNP protein, is thought to be a central regulator of DEAD-box proteins, we conducted a targeted ..
  41. Lacadie S, Rosbash M. Cotranscriptional spliceosome assembly dynamics and the role of U1 snRNA:5'ss base pairing in yeast. Mol Cell. 2005;19:65-75 pubmed
    ..A 5'ss-U1 snRNA complementation experiment suggests that pairing between U1 and the 5'ss occurs after U1 recruitment and contributes to a specific U1:substrate conformation required for efficient U2 and tri-snRNP recruitment. ..
  42. Schneider C, Agafonov D, Schmitzová J, Hartmuth K, Fabrizio P, Lührmann R. Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes. PLoS Genet. 2015;11:e1005539 pubmed publisher
    ..Upon step 1 catalysis, Cwc25 contacts with the branch-site region, and enhanced crosslinks of Prp8 and Prp45 with nucleotides surrounding the branch-site were observed...
  43. Millet C, Ausiannikava D, Le Bihan T, Granneman S, Makovets S. Cell populations can use aneuploidy to survive telomerase insufficiency. Nat Commun. 2015;6:8664 pubmed publisher
    ..The aneuploidy-induced re-balance of the proteome via modulation of ribosome biogenesis may be a general adaptive response to overcome functional insufficiencies. ..
  44. Gahura O, Abrhámová K, Skruzny M, Valentová A, Munzarová V, Folk P, et al. Prp45 affects Prp22 partition in spliceosomal complexes and splicing efficiency of non-consensus substrates. J Cell Biochem. 2009;106:139-51 pubmed publisher
    ..Our data suggest that Prp45 contributes, in part through its interaction with the 2nd step-proofreading helicase Prp22, to splicing efficiency of substrates non-conforming to the consensus. ..
  45. Absmeier E, Becke C, Wollenhaupt J, Santos K, Wahl M. Interplay of cis- and trans-regulatory mechanisms in the spliceosomal RNA helicase Brr2. Cell Cycle. 2017;16:100-112 pubmed publisher
    ..Furthermore, it can be regulated in trans by the Jab1 domain of the Prp8 protein, which can inhibit Brr2 by intermittently inserting a C-terminal tail in the enzyme's RNA-binding tunnel or ..
  46. Umen J, Guthrie C. Prp16p, Slu7p, and Prp8p interact with the 3' splice site in two distinct stages during the second catalytic step of pre-mRNA splicing. RNA. 1995;1:584-97 pubmed
    ..b>prp8-101, an allele of PRP8 defective in 3' splice site recognition, exhibits specific genetic interactions with mutant ..
  47. Awasthi S, Palmer R, Castro M, Mobarak C, Ruby S. New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing. J Biol Chem. 2001;276:31004-15 pubmed
    ..The mutation also led to a defect in splicing and prespliceosome formation in vitro; an indication that Exo84p has a direct role in splicing. The results elucidate a surprising link between splicing and secretion. ..
  48. Aronova A, Bacikova D, Crotti L, Horowitz D, Schwer B. Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing. RNA. 2007;13:1437-44 pubmed
    ..the Prp22 helicase catalyzes release of spliced mRNA by disrupting contacts in the spliceosome that likely involve Prp8. Mutations at Arg1753 in Prp8, which suppress helicase-defective prp22 mutants, elicit temperature-sensitive growth ..
  49. Grainger R, Barrass J, Jacquier A, Rain J, Beggs J. Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome. RNA. 2009;15:2161-73 pubmed publisher
    ..Complementary chemical cross-linking experiments reveal reciprocal protein footprints between the interacting Prp8 and Cwc21 proteins, identifying the conserved cwf21 domain in Cwc21p as a Prp8p binding site...
  50. Nancollis V, Ruckshanthi J, Frazer L, O Keefe R. The U5 snRNA internal loop 1 is a platform for Brr2, Snu114 and Prp8 protein binding during U5 snRNP assembly. J Cell Biochem. 2013;114:2770-84 pubmed publisher
    ..The proteins Prp8, Snu114 and Brr2 all assemble with the U5 small nuclear RNA (snRNA) to produce the U5 snRNP...
  51. Dreumont N, Seraphin B. Rapid screening of yeast mutants with reporters identifies new splicing phenotypes. FEBS J. 2013;280:2712-26 pubmed publisher
    ..Interestingly, a mutant of PRP8 specifically lacking an N-terminal proline-rich region stimulated the splicing of a reporter containing competing ..
  52. Chiang T, Cheng S. A weak spliceosome-binding domain of Yju2 functions in the first step and bypasses Prp16 in the second step of splicing. Mol Cell Biol. 2013;33:1746-55 pubmed publisher
  53. Abovich N, Rosbash M. Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals. Cell. 1997;89:403-12 pubmed
    ..Our results show that SF1 interacts strongly with human U2AF65, and that SF1 is a bona fide E complex component. This implies that aspects of these novel cross-intron protein-protein interactions are conserved between yeast and mammals. ..
  54. Boon K, Auchynnikava T, Edwalds Gilbert G, Barrass J, Droop A, Dez C, et al. Yeast ntr1/spp382 mediates prp43 function in postspliceosomes. Mol Cell Biol. 2006;26:6016-23 pubmed
    ..extract, and increased levels of U2 and decreased levels of U4 are found associated with the U5 snRNP protein Prp8. These results suggest a requirement for Ntr1 for turnover of the excised intron complex and recycling of snRNPs...
  55. Strauss E, Guthrie C. A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family. Genes Dev. 1991;5:629-41 pubmed
    ..In addition, a suppressor of prp28-1 is a mutant allele of PRP8, which encodes a U5 protein, thus linking PRP28 with U5...
  56. Ben Yehuda S, Russell C, Dix I, Beggs J, Kupiec M. Extensive genetic interactions between PRP8 and PRP17/CDC40, two yeast genes involved in pre-mRNA splicing and cell cycle progression. Genetics. 2000;154:61-71 pubmed
    ..In this report we describe extensive genetic interactions between PRP17/CDC40 and the PRP8 gene...
  57. Nguyen T, Li J, Galej W, Oshikane H, Newman A, Nagai K. Structural basis of Brr2-Prp8 interactions and implications for U5 snRNP biogenesis and the spliceosome active site. Structure. 2013;21:910-19 pubmed
    ..Here, we present the structure of yeast Brr2 in complex with the Jab1/MPN domain of Prp8, which stimulates Brr2 activity...
  58. Gautam A, Grainger R, Vilardell J, Barrass J, Beggs J. Cwc21p promotes the second step conformation of the spliceosome and modulates 3' splice site selection. Nucleic Acids Res. 2015;43:3309-17 pubmed publisher
    ..Here, we show that mutations in PRP16, PRP8, SNU114 and the U5 snRNA that affect this process interact genetically with CWC21, that encodes the yeast ..
  59. Ben Yehuda S, Dix I, Russell C, McGarvey M, Beggs J, Kupiec M. Genetic and physical interactions between factors involved in both cell cycle progression and pre-mRNA splicing in Saccharomyces cerevisiae. Genetics. 2000;156:1503-17 pubmed
    ..We discuss the role played by these proteins in splicing and cell cycle progression. ..