PRP43

Summary

Gene Symbol: PRP43
Description: DEAH-box ATP-dependent RNA helicase PRP43
Alias: DEAH-box ATP-dependent RNA helicase PRP43
Species: Saccharomyces cerevisiae S288c
Products:     PRP43

Top Publications

  1. Lebaron S, Papin C, Capeyrou R, Chen Y, Froment C, Monsarrat B, et al. The ATPase and helicase activities of Prp43p are stimulated by the G-patch protein Pfa1p during yeast ribosome biogenesis. EMBO J. 2009;28:3808-19 pubmed publisher
    ..Our results strongly suggest that stimulation of ATPase/helicase activities of Prp43p by Pfa1p is required for efficient 20S pre-rRNA-to-18S rRNA conversion. ..
  2. Tanaka N, Aronova A, Schwer B. Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome. Genes Dev. 2007;21:2312-25 pubmed
    ..The RNA-dependent NTPase Prp43 catalyzes dissociation of excised lariat-intron from the spliceosome, but it is unclear how Prp43 couples the ..
  3. Tsai R, Fu R, Yeh F, Tseng C, Lin Y, Huang Y, et al. Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2. Genes Dev. 2005;19:2991-3003 pubmed
    ..Immunoprecipitation analysis revealed that Ntr1 and Ntr2 formed a stable complex with DExD/H-box RNA helicase Prp43 in the splicing extract...
  4. Boon K, Auchynnikava T, Edwalds Gilbert G, Barrass J, Droop A, Dez C, et al. Yeast ntr1/spp382 mediates prp43 function in postspliceosomes. Mol Cell Biol. 2006;26:6016-23 pubmed
    ..Ntr1 interacts directly or indirectly with the intron release factor Prp43 and is required for its association with the excised intron...
  5. Tanaka N, Schwer B. Mutations in PRP43 that uncouple RNA-dependent NTPase activity and pre-mRNA splicing function. Biochemistry. 2006;45:6510-21 pubmed
    Saccharomyces cerevisiae Prp43 is a DEAH-box RNA-dependent ATPase that catalyzes the release of excised lariat intron from the mRNA spliceosome...
  6. Christian H, Hofele R, Urlaub H, Ficner R. Insights into the activation of the helicase Prp43 by biochemical studies and structural mass spectrometry. Nucleic Acids Res. 2014;42:1162-79 pubmed publisher
    ..The DEAH-box protein Prp43 is responsible for the disassembly of the intron-lariat spliceosome and its helicase activity is activated by the ..
  7. Walbott H, Mouffok S, Capeyrou R, Lebaron S, Humbert O, van Tilbeurgh H, et al. Prp43p contains a processive helicase structural architecture with a specific regulatory domain. EMBO J. 2010;29:2194-204 pubmed publisher
    ..We propose that the C-terminal domain, specific to DEAH/RHA helicases, is a central player in the regulation of helicase activity by binding both RNA and G-patch domain proteins. ..
  8. Bohnsack M, Martin R, Granneman S, Ruprecht M, Schleiff E, Tollervey D. Prp43 bound at different sites on the pre-rRNA performs distinct functions in ribosome synthesis. Mol Cell. 2009;36:583-92 pubmed publisher
    ..Here we identify multiple binding sites for the helicase Prp43 in the 18S and 25S rRNA regions of pre-rRNAs, using UV crosslinking...
  9. Combs D, Nagel R, Ares M, Stevens S. Prp43p is a DEAH-box spliceosome disassembly factor essential for ribosome biogenesis. Mol Cell Biol. 2006;26:523-34 pubmed
    ..Conditional prp43 mutant alleles confer a 35S pre-rRNA processing defect, with subsequent depletion of 27S and 20S precursors...

More Information

Publications33

  1. Tsai R, Tseng C, Lee P, Chen H, Fu R, Chang K, et al. Dynamic interactions of Ntr1-Ntr2 with Prp43 and with U5 govern the recruitment of Prp43 to mediate spliceosome disassembly. Mol Cell Biol. 2007;27:8027-37 pubmed
    ..Ntr1 (also known as Spp382) and Ntr2 form a stable complex and can further associate with DExD/H-box RNA helicase Prp43 to form a functional complex, termed the NTR complex, which catalyzes spliceosome disassembly...
  2. Leeds N, Small E, Hiley S, Hughes T, Staley J. The splicing factor Prp43p, a DEAH box ATPase, functions in ribosome biogenesis. Mol Cell Biol. 2006;26:513-22 pubmed
    ..By steady-state analysis, a cold-sensitive prp43 mutant accumulates 35S pre-rRNA and depletes 20S, 27S, and 7S pre-rRNAs, precursors to the small- and large-..
  3. Pertschy B, Schneider C, Gnädig M, Schäfer T, Tollervey D, Hurt E. RNA helicase Prp43 and its co-factor Pfa1 promote 20 to 18 S rRNA processing catalyzed by the endonuclease Nob1. J Biol Chem. 2009;284:35079-91 pubmed publisher
    ..requires functional interactions between PIN (PilT N terminus) domain protein Nob1 and the DEAH box RNA helicase Prp43. Nob1 showed specific cleavage on a D-site substrate analogue in vitro, which was abolished by mutations in the ..
  4. Martin A, Schneider S, Schwer B. Prp43 is an essential RNA-dependent ATPase required for release of lariat-intron from the spliceosome. J Biol Chem. 2002;277:17743-50 pubmed
    The essential Saccharomyces cerevisiae PRP43 gene encodes a 767-amino acid protein of the DEXH-box family. Prp43 has been implicated in spliceosome disassembly (Arenas, J. E., and Abelson, J. N. (1997) Proc. Natl. Acad. Sci. U. S. A...
  5. Pandit S, Lynn B, Rymond B. Inhibition of a spliceosome turnover pathway suppresses splicing defects. Proc Natl Acad Sci U S A. 2006;103:13700-5 pubmed
    ..Consistent with a related function in the removal of defective complexes, three prp43 mutants are also shown to suppress splicing defects, with efficiencies inversely proportionate to the measured ..
  6. Koodathingal P, Novak T, Piccirilli J, Staley J. The DEAH box ATPases Prp16 and Prp43 cooperate to proofread 5' splice site cleavage during pre-mRNA splicing. Mol Cell. 2010;39:385-95 pubmed publisher
    ..rejection is reversible, necessitating a downstream discard pathway that we found requires the DEAH box ATPase Prp43, a spliceosome disassembly factor...
  7. Carrocci T, Zoerner D, Paulson J, Hoskins A. SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast. Nucleic Acids Res. 2017;45:4837-4852 pubmed publisher
    ..We propose that SF3b1 functions to stabilize weak U2/BS duplexes to drive spliceosome assembly and splicing. ..
  8. Hackmann A, Wu H, Schneider U, Meyer K, Jung K, Krebber H. Quality control of spliced mRNAs requires the shuttling SR proteins Gbp2 and Hrb1. Nat Commun. 2014;5:3123 pubmed publisher
    ..Altogether, these data identify a role for shuttling SR proteins in mRNA surveillance and nuclear mRNA quality control. ..
  9. Kong K, Tang H, Pan K, Huang Z, Lee T, Hinnebusch A, et al. Cotranscriptional recruitment of yeast TRAMP complex to intronic sequences promotes optimal pre-mRNA splicing. Nucleic Acids Res. 2014;42:643-60 pubmed publisher
  10. Fourmann J, Dybkov O, Agafonov D, Tauchert M, Urlaub H, Ficner R, et al. The target of the DEAH-box NTP triphosphatase Prp43 in Saccharomyces cerevisiae spliceosomes is the U2 snRNP-intron interaction. elife. 2016;5: pubmed publisher
    The DEAH-box NTPase Prp43 and its cofactors Ntr1 and Ntr2 form the NTR complex and are required for disassembling intron-lariat spliceosomes (ILS) and defective earlier spliceosomes...
  11. Luz J, Georg R, Gomes C, Machado Santelli G, Oliveira C. Sdo1p, the yeast orthologue of Shwachman-Bodian-Diamond syndrome protein, binds RNA and interacts with nuclear rRNA-processing factors. Yeast. 2009;26:287-98 pubmed publisher
    ..Sdo1p binds RNA directly, suggesting that it may associate with the ribosomal subunits also through RNA interaction. ..
  12. Pandit S, Paul S, Zhang L, Chen M, Durbin N, Harrison S, et al. Spp382p interacts with multiple yeast splicing factors, including possible regulators of Prp43 DExD/H-Box protein function. Genetics. 2009;183:195-206 pubmed publisher
    ..These and related data establish the value of this dosage interference assay for finding genes that alter cellular splicing and define Sqs1p and Cwc23p as prospective modulators of Spp382p-stimuated Prp43p function. ..
  13. Heininger A, Hackert P, Andreou A, Boon K, Memet I, Prior M, et al. Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways. RNA Biol. 2016;13:320-30 pubmed publisher
    ..Here, we show that the distribution of the multifunctional DEAH-box RNA helicase Prp43 between its diverse cellular functions can be regulated by the interplay of its G-patch protein cofactors...
  14. Mayas R, Maita H, Semlow D, Staley J. Spliceosome discards intermediates via the DEAH box ATPase Prp43p. Proc Natl Acad Sci U S A. 2010;107:10020-5 pubmed publisher
    ..Because Prp43p also promotes spliceosome disassembly after exon ligation, this work establishes a parallel between the discard of suboptimal intermediates and the dissociation of a genuine excised intron product. ..
  15. Fourmann J, Tauchert M, Ficner R, Fabrizio P, Luhrmann R. Regulation of Prp43-mediated disassembly of spliceosomes by its cofactors Ntr1 and Ntr2. Nucleic Acids Res. 2017;45:4068-4080 pubmed publisher
    The DEAH-box NTPase Prp43 disassembles spliceosomes in co-operation with the cofactors Ntr1/Spp382 and Ntr2, forming the NTR complex...
  16. Moriggi G, Nieto B, Dosil M. Rrp12 and the Exportin Crm1 participate in late assembly events in the nucleolus during 40S ribosomal subunit biogenesis. PLoS Genet. 2014;10:e1004836 pubmed publisher
  17. Chen Y, Capeyrou R, Humbert O, Mouffok S, Kadri Y, Lebaron S, et al. The telomerase inhibitor Gno1p/PINX1 activates the helicase Prp43p during ribosome biogenesis. Nucleic Acids Res. 2014;42:7330-45 pubmed publisher
    ..Moreover, PINX1 interacts with human PRP43 (DHX15) in HeLa cells...
  18. Robert Paganin J, Halladjian M, Blaud M, Lebaron S, Delbos L, Chardon F, et al. Functional link between DEAH/RHA helicase Prp43 activation and ATP base binding. Nucleic Acids Res. 2017;45:1539-1552 pubmed publisher
    The DEAH box helicase Prp43 is a bifunctional enzyme from the DEAH/RHA helicase family required both for the maturation of ribosomes and for lariat intron release during splicing...
  19. McCann K, Teramoto T, Zhang J, Tanaka Hall T, Baserga S. The molecular basis for ANE syndrome revealed by the large ribosomal subunit processome interactome. elife. 2016;5: pubmed publisher
  20. Blair L, Liu Z, Labitigan R, Wu L, Zheng D, Xia Z, et al. KDM5 lysine demethylases are involved in maintenance of 3'UTR length. Sci Adv. 2016;2:e1501662 pubmed publisher
    ..In contrast, both KDM5A and KDM5B are involved in the lengthening of DICER1. Our findings suggest both a novel role for this family of demethylases and a novel targetable mechanism for 3'UTR processing. ..
  21. Banerjee D, McDaniel P, Rymond B. Limited portability of G-patch domains in regulators of the Prp43 RNA helicase required for pre-mRNA splicing and ribosomal RNA maturation in Saccharomyces cerevisiae. Genetics. 2015;200:135-47 pubmed publisher
    The Prp43 DExD/H-box protein is required for progression of the biochemically distinct pre-messenger RNA and ribosomal RNA (rRNA) maturation pathways...
  22. Neves L, Douglass S, Spreafico R, Venkataramanan S, Kress T, Johnson T. The histone variant H2A.Z promotes efficient cotranscriptional splicing in S. cerevisiae. Genes Dev. 2017;31:702-717 pubmed publisher
    ..Z's role in elongation. Depletion of disassembly factor Prp43 suppresses H2A.Z-mediated splice defects, indicating that, in the absence of H2A...
  23. Sahi C, Lee T, Inada M, Pleiss J, Craig E. Cwc23, an essential J protein critical for pre-mRNA splicing with a dispensable J domain. Mol Cell Biol. 2010;30:33-42 pubmed publisher
    ..interactions were uncovered between point mutations encoding alterations in Cwc23's J domain and either Ntr1 or Prp43, a DExD/H-box helicase essential for spliceosome disassembly...
  24. He Y, Staley J, Andersen G, Nielsen K. Structure of the DEAH/RHA ATPase Prp43p bound to RNA implicates a pair of hairpins and motif Va in translocation along RNA. RNA. 2017;23:1110-1124 pubmed publisher
    ..Our crystal structure, genetic, and biochemical experiments, as well as comparisons with other DExH ATPases, support a generalized mechanism for the DExH class of helicases involving a pair of bookends that inchworm along RNA. ..