Gene Symbol: PRP40
Description: Prp40p
Alias: Prp40p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Gottschalk A, Tang J, Puig O, Salgado J, Neubauer G, Colot H, et al. A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins. RNA. 1998;4:374-93 pubmed
    ..Finally, we show that Nam8p/Mud15p contributes to the stability of U1 snRNP. ..
  2. Abovich N, Rosbash M. Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals. Cell. 1997;89:403-12 pubmed
    ..and biochemical criteria indicate a direct interaction between Msl5p and both Mud2p and the U1 snRNP protein Prp40p. This defines a bridge between the two ends of the intron...
  3. Gasch A, Wiesner S, Martin Malpartida P, Ramirez Espain X, Ruiz L, Macias M. The structure of Prp40 FF1 domain and its interaction with the crn-TPR1 motif of Clf1 gives a new insight into the binding mode of FF domains. J Biol Chem. 2006;281:356-64 pubmed
    The yeast splicing factor Prp40 (pre-mRNA processing protein 40) consists of a pair of WW domains followed by several FF domains...
  4. Chung S, McLean M, Rymond B. Yeast ortholog of the Drosophila crooked neck protein promotes spliceosome assembly through stable U4/U6.U5 snRNP addition. RNA. 1999;5:1042-54 pubmed
    ..analyses and in vitro binding studies show that Clf1p interacts specifically and differentially with the U1 snRNP-Prp40p protein and the yeast U2AF65 homolog, Mud2p...
  5. Fortes P, Bilbao Cortes D, Fornerod M, Rigaut G, Raymond W, Seraphin B, et al. Luc7p, a novel yeast U1 snRNP protein with a role in 5' splice site recognition. Genes Dev. 1999;13:2425-38 pubmed
    ..These data suggest that the loss of Luc7p disrupts U1 snRNP-CBC interaction, and that this interaction contributes to normal 5' splice site recognition. ..
  6. Morris D, Greenleaf A. The splicing factor, Prp40, binds the phosphorylated carboxyl-terminal domain of RNA polymerase II. J Biol Chem. 2000;275:39935-43 pubmed
    ..binding by four other WW domain-containing Saccharomyces cerevisiae proteins indicates the splicing factor, Prp40, and the RNA polymerase II ubiquitin ligase, Rsp5, can also bind the phospho-CTD...
  7. Murphy M, Olson B, Siliciano P. The yeast splicing factor Prp40p contains functional leucine-rich nuclear export signals that are essential for splicing. Genetics. 2004;166:53-65 pubmed
    To investigate the function of the essential U1 snRNP protein Prp40p, we performed a synthetic lethal screen in Saccharomyces cerevisiae...
  8. Becerra S, Andrés León E, Prieto Sánchez S, Hernández Munain C, Suñé C. Prp40 and early events in splice site definition. Wiley Interdiscip Rev RNA. 2016;7:17-32 pubmed publisher
    ..We also discuss the role of the essential yeast Prp40 protein and its mammalian homologs in the specificity of this pre-mRNA processing event...
  9. Kao H, Siliciano P. Identification of Prp40, a novel essential yeast splicing factor associated with the U1 small nuclear ribonucleoprotein particle. Mol Cell Biol. 1996;16:960-7 pubmed
    ..In this way, we isolated PRP40-1, a suppressor that restores growth at 18 degrees C to a strain bearing a cold-sensitive mutation in U1 RNA...

More Information


  1. Ester C, Uetz P. The FF domains of yeast U1 snRNP protein Prp40 mediate interactions with Luc7 and Snu71. BMC Biochem. 2008;9:29 pubmed publisher
    ..Saccharomyces cerevisiae encodes two FF domain proteins, Prp40, a component of the U1 snRNP, and Ypr152c, a protein of unknown function...
  2. Hoskins A, Friedman L, Gallagher S, Crawford D, Anderson E, Wombacher R, et al. Ordered and dynamic assembly of single spliceosomes. Science. 2011;331:1289-95 pubmed publisher
    ..This experimental strategy should prove widely useful for mechanistic analysis of other macromolecular machines in environments approaching the complexity of living cells. ..
  3. Agarwal R, Schwer B, Shuman S. Structure-function analysis and genetic interactions of the Luc7 subunit of the Saccharomyces cerevisiae U1 snRNP. RNA. 2016;22:1302-10 pubmed publisher
  4. Price A, Görnemann J, Guthrie C, Brow D. An unanticipated early function of DEAD-box ATPase Prp28 during commitment to splicing is modulated by U5 snRNP protein Prp8. RNA. 2014;20:46-60 pubmed publisher
    ..We conclude that the U5 snRNP has a role in the earliest events of assembly, prior to its stable incorporation into the spliceosome. ..
  5. Hoskins A, Rodgers M, Friedman L, Gelles J, Moore M. Single molecule analysis reveals reversible and irreversible steps during spliceosome activation. elife. 2016;5: pubmed publisher
    ..The data reveal the activation mechanism and show that overall splicing efficiency may be maintained through repeated rounds of disassembly and tri-snRNP reassociation. ..
  6. Rigaut G, Shevchenko A, Rutz B, Wilm M, Mann M, Seraphin B. A generic protein purification method for protein complex characterization and proteome exploration. Nat Biotechnol. 1999;17:1030-2 pubmed
    ..Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms. ..
  7. Phatnani H, Jones J, Greenleaf A. Expanding the functional repertoire of CTD kinase I and RNA polymerase II: novel phosphoCTD-associating proteins in the yeast proteome. Biochemistry. 2004;43:15702-19 pubmed
  8. Görnemann J, Barrandon C, Hujer K, Rutz B, Rigaut G, Kotovic K, et al. Cotranscriptional spliceosome assembly and splicing are independent of the Prp40p WW domain. RNA. 2011;17:2119-29 pubmed publisher
    ..In yeast, the U1 snRNP subunit Prp40 was proposed to mediate cotranscriptional recruitment of early splicing factors through binding of its WW domains ..
  9. Moore M, Schwartzfarb E, Silver P, Yu M. Differential recruitment of the splicing machinery during transcription predicts genome-wide patterns of mRNA splicing. Mol Cell. 2006;24:903-15 pubmed
    ..Broadly, our results provide mechanistic insights into the coordinated regulation of transcription, mRNA processing, and nuclear export in executing complex gene expression programs. ..
  10. Rodgers M, Paulson J, Hoskins A. Rapid isolation and single-molecule analysis of ribonucleoproteins from cell lysate by SNAP-SiMPull. RNA. 2015;21:1031-41 pubmed publisher
    ..SNAP-SiMPull will likely find broad use for rapidly isolating complex cellular machines for single-molecule fluorescence colocalization experiments. ..
  11. Bonet R, Ruiz L, Morales B, Macias M. Solution structure of the fourth FF domain of yeast Prp40 splicing factor. Proteins. 2009;77:1000-3 pubmed publisher
  12. Yeh C, Chang S, Chen J, Wang H, Chou Y, Wang C, et al. The conserved AU dinucleotide at the 5' end of nascent U1 snRNA is optimized for the interaction with nuclear cap-binding-complex. Nucleic Acids Res. 2017;45:9679-9693 pubmed publisher
    ..Our data also provide a structural interpretation as to why the AU dinucleotide is conserved during evolution. ..