Gene Symbol: PRP4
Description: U4/U6-U5 snRNP complex subunit PRP4
Alias: RNA4, U4/U6-U5 snRNP complex subunit PRP4
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Hu J, Xu Y, Schappert K, Harrington T, Wang A, Braga R, et al. Mutational analysis of the PRP4 protein of Saccharomyces cerevisiae suggests domain structure and snRNP interactions. Nucleic Acids Res. 1994;22:1724-34 pubmed
    The PRP4 protein of Saccharomyces cerevisiae is an essential part of the U4/U6 snRNP, a component of the mRNA splicing apparatus...
  2. Bouveret E, Rigaut G, Shevchenko A, Wilm M, Seraphin B. A Sm-like protein complex that participates in mRNA degradation. EMBO J. 2000;19:1661-71 pubmed
    ..These results indicate the involvement of a new conserved Sm-like protein complex and a new factor, Pat1p, in mRNA degradation and suggest a physical connection between decapping and exonuclease trimming. ..
  3. Häcker I, Sander B, Golas M, Wolf E, Karagöz E, Kastner B, et al. Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy. Nat Struct Mol Biol. 2008;15:1206-12 pubmed publisher
    ..The head and arm adopt variable relative positions. This molecular organization and dynamics suggest possible scenarios for structural events during catalytic activation. ..
  4. Last R, Maddock J, Woolford J. Evidence for related functions of the RNA genes of Saccharomyces cerevisiae. Genetics. 1987;117:619-31 pubmed
    ..The presence of extra plasmid-borne copies of the RNA3 gene relieves the lethality of temperature-sensitive rna4 mutations...
  5. Maddock J, Weidenhammer E, Adams C, Lunz R, Woolford J. Extragenic suppressors of Saccharomyces cerevisiae prp4 mutations identify a negative regulator of PRP genes. Genetics. 1994;136:833-47 pubmed
    The PRP4 gene encodes a protein that is a component of the U4/U6 small nuclear ribonucleoprotein particle and is necessary for both spliceosome assembly and pre-mRNA splicing...
  6. Boon K, Norman C, Grainger R, Newman A, Beggs J. Prp8p dissection reveals domain structure and protein interaction sites. RNA. 2006;12:198-205 pubmed
    ..Thus, the U5 snRNP protein Snu114p associates with Prp8p in the region 437-770, whereas fragmenting Prp8p at residue 2173 destabilizes its association with Aar2p. ..
  7. Düring L, Thorsen M, Petersen D, Køster B, Jensen T, Holmberg S. MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation. PLoS ONE. 2012;7:e44373 pubmed publisher
    ..Genetic interactions are observed between 2 µm-MRN1 and the splicing deficient mutants snt309?, prp3, prp4, and prp22, and additional genetic analyses link MRN1, SNT309, NHP6A/B, SWI/SNF, and RSC supporting the notion of a ..
  8. Dreumont N, Seraphin B. Rapid screening of yeast mutants with reporters identifies new splicing phenotypes. FEBS J. 2013;280:2712-26 pubmed publisher
    ..Thus, using appropriate reporters, yeast can be used to quickly delineate the effect of various factors on splicing and identify those with the propensity to regulate alternative splicing events. ..
  9. Huang Y, Chung C, Kao D, Kao T, Cheng S. Sad1 counteracts Brr2-mediated dissociation of U4/U6.U5 in tri-snRNP homeostasis. Mol Cell Biol. 2014;34:210-20 pubmed publisher
    ..Our results demonstrate a role of Sad1 in maintaining the integrity of the tri-snRNP by counteracting Brr2-mediated dissociation of tri-snRNP and provide insights into homeostasis of the tri-snRNP. ..

More Information


  1. Hoskins A, Rodgers M, Friedman L, Gelles J, Moore M. Single molecule analysis reveals reversible and irreversible steps during spliceosome activation. elife. 2016;5: pubmed publisher
    ..The data reveal the activation mechanism and show that overall splicing efficiency may be maintained through repeated rounds of disassembly and tri-snRNP reassociation. ..
  2. Fabrizio P, Esser S, Kastner B, Luhrmann R. Isolation of S. cerevisiae snRNPs: comparison of U1 and U4/U6.U5 to their human counterparts. Science. 1994;264:261-5 pubmed
    ..U5 snRNPs are significantly similar. The preparative isolation of yeast snRNPs will allow the cloning as well as genetic and phylogenetic analysis of snRNP proteins which will accelerate our understanding of their function. ..
  3. Ayadi L, Callebaut I, Saguez C, Villa T, Mornon J, Banroques J. Functional and structural characterization of the prp3 binding domain of the yeast prp4 splicing factor. J Mol Biol. 1998;284:673-87 pubmed
    ..The yeast Prp4 (yPrp4) protein is a specific component of the U4/U6 and U4/U6-U5 snRNPs, which associates transiently with the ..
  4. Bracken A, Bond U. Reassembly and protection of small nuclear ribonucleoprotein particles by heat shock proteins in yeast cells. RNA. 1999;5:1586-96 pubmed
    ..In addition, our results show that Hsp70 plays a role in snRNP assembly under normal physiological conditions. ..
  5. Chen C, Yu W, Tsao T, Wang L, Chen H, Lin J, et al. Functional and physical interactions between components of the Prp19p-associated complex. Nucleic Acids Res. 2002;30:1029-37 pubmed
    ..Genetic analysis suggests that Ntc31p, Ntc30p and Ntc20p may play roles in modulating the function of Ntc90p. ..
  6. Taoka M, Yamauchi Y, Nobe Y, Masaki S, Nakayama H, Ishikawa H, et al. An analytical platform for mass spectrometry-based identification and chemical analysis of RNA in ribonucleoprotein complexes. Nucleic Acids Res. 2009;37:e140 pubmed publisher
  7. Munding E, Shiue L, Katzman S, Donohue J, Ares M. Competition between pre-mRNAs for the splicing machinery drives global regulation of splicing. Mol Cell. 2013;51:338-48 pubmed publisher
    ..of the RPG-dedicated transcription factor gene IFH1 genetically suppresses two spliceosome mutations, prp11-1 and prp4-1, and globally restores splicing efficiency in prp4-1 cells...