Gene Symbol: PRP3
Description: U4/U6-U5 snRNP complex subunit PRP3
Alias: RNA3, U4/U6-U5 snRNP complex subunit PRP3
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Maddock J, Weidenhammer E, Adams C, Lunz R, Woolford J. Extragenic suppressors of Saccharomyces cerevisiae prp4 mutations identify a negative regulator of PRP genes. Genetics. 1994;136:833-47 pubmed
    ..Each of these spp mutations also suppresses prp3; spp41 and spp42 suppress prp11 as well...
  2. Fujimura H. Molecular cloning of Saccharomyces cerevisiae MGC1/YDR473c gene which is essential for cell growth. Antonie Van Leeuwenhoek. 2000;77:65-9 pubmed
    ..Protein-protein interaction of Mgc1 protein was shown by using yeast two-hybrid system. Mgc1 protein was also proposed to be localized in the nucleus in yeast cells. ..
  3. Bouveret E, Rigaut G, Shevchenko A, Wilm M, Seraphin B. A Sm-like protein complex that participates in mRNA degradation. EMBO J. 2000;19:1661-71 pubmed
    ..These results indicate the involvement of a new conserved Sm-like protein complex and a new factor, Pat1p, in mRNA degradation and suggest a physical connection between decapping and exonuclease trimming. ..
  4. Häcker I, Sander B, Golas M, Wolf E, Karagöz E, Kastner B, et al. Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy. Nat Struct Mol Biol. 2008;15:1206-12 pubmed publisher
    ..The head and arm adopt variable relative positions. This molecular organization and dynamics suggest possible scenarios for structural events during catalytic activation. ..
  5. Wang R, Solomon M. Identification of She3 as an SCF(Grr1) substrate in budding yeast. PLoS ONE. 2012;7:e48020 pubmed publisher
    ..However, She3 stabilization leads to slower growth compared to wild-type cells in a co-culture assay, demonstrating that the degradation of She3 by Grr1 is required for optimal cell growth. ..
  6. Dreumont N, Seraphin B. Rapid screening of yeast mutants with reporters identifies new splicing phenotypes. FEBS J. 2013;280:2712-26 pubmed publisher
    ..Thus, using appropriate reporters, yeast can be used to quickly delineate the effect of various factors on splicing and identify those with the propensity to regulate alternative splicing events. ..
  7. Last R, Maddock J, Woolford J. Evidence for related functions of the RNA genes of Saccharomyces cerevisiae. Genetics. 1987;117:619-31 pubmed
    ..The presence of extra plasmid-borne copies of the RNA3 gene relieves the lethality of temperature-sensitive rna4 mutations...
  8. Ayadi L, Callebaut I, Saguez C, Villa T, Mornon J, Banroques J. Functional and structural characterization of the prp3 binding domain of the yeast prp4 splicing factor. J Mol Biol. 1998;284:673-87 pubmed
    ..This surface could be used as a scaffold for generating an RNA-protein complex. ..
  9. Boon K, Norman C, Grainger R, Newman A, Beggs J. Prp8p dissection reveals domain structure and protein interaction sites. RNA. 2006;12:198-205 pubmed
    ..Thus, the U5 snRNP protein Snu114p associates with Prp8p in the region 437-770, whereas fragmenting Prp8p at residue 2173 destabilizes its association with Aar2p. ..

More Information


  1. Düring L, Thorsen M, Petersen D, Køster B, Jensen T, Holmberg S. MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation. PLoS ONE. 2012;7:e44373 pubmed publisher
    ..Genetic interactions are observed between 2 µm-MRN1 and the splicing deficient mutants snt309?, prp3, prp4, and prp22, and additional genetic analyses link MRN1, SNT309, NHP6A/B, SWI/SNF, and RSC supporting the ..
  2. Liu S, Mozaffari Jovin S, Wollenhaupt J, Santos K, Theuser M, Dunin Horkawicz S, et al. A composite double-/single-stranded RNA-binding region in protein Prp3 supports tri-snRNP stability and splicing. elife. 2015;4:e07320 pubmed publisher
    b>Prp3 is an essential U4/U6 di-snRNP-associated protein whose functions and molecular mechanisms in pre-mRNA splicing are presently poorly understood...
  3. Hoskins A, Rodgers M, Friedman L, Gelles J, Moore M. Single molecule analysis reveals reversible and irreversible steps during spliceosome activation. elife. 2016;5: pubmed publisher
    ..The data reveal the activation mechanism and show that overall splicing efficiency may be maintained through repeated rounds of disassembly and tri-snRNP reassociation. ..