Gene Symbol: PRP22
Description: DEAH-box ATP-dependent RNA helicase PRP22
Alias: DEAH-box ATP-dependent RNA helicase PRP22
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Ohtani M, Demura T, Sugiyama M. Arabidopsis root initiation defective1, a DEAH-box RNA helicase involved in pre-mRNA splicing, is essential for plant development. Plant Cell. 2013;25:2056-69 pubmed publisher
    ..Our results collectively suggest that robust levels of pre-mRNA splicing are critical for several specific aspects of plant development...
  2. Kawashima T, Pellegrini M, Chanfreau G. Nonsense-mediated mRNA decay mutes the splicing defects of spliceosome component mutations. RNA. 2009;15:2236-47 pubmed publisher
    ..Inactivation of Upf1p in the second step/recycling factor prp22-1 mutant and in the nam8Delta and mud1Delta U1 snRNP component mutants also increase unspliced precursor ..
  3. Daoud R, Forget L, Lang B. Yeast mitochondrial RNase P, RNase Z and the RNA degradosome are part of a stable supercomplex. Nucleic Acids Res. 2012;40:1728-36 pubmed publisher
    ..Finally, the question of mt-RNase P localization within mitochondria was investigated, by GFP-tracing of a known protein subunit (Rpm2p). We find that about equal fractions of RNase P are soluble versus membrane-attached. ..
  4. Query C, Konarska M. CEF1/CDC5 alleles modulate transitions between catalytic conformations of the spliceosome. RNA. 2012;18:1001-13 pubmed publisher
  5. Wagner J, Jankowsky E, Company M, Pyle A, Abelson J. The DEAH-box protein PRP22 is an ATPase that mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes. EMBO J. 1998;17:2926-37 pubmed
    ..However, the mechanism for the proposed alterations is presently unknown. We present evidence that PRP22, a DEAH-box protein required for mRNA release from the spliceosome, unwinds RNA duplexes in a concentration- and ..
  6. Schwer B, Gross C. Prp22, a DExH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicing. EMBO J. 1998;17:2086-94 pubmed
    In order to assess the role of Prp22 in yeast pre-mRNA splicing, we have purified the 130 kDa Prp22 protein and developed an in vitro depletion/reconstitution assay...
  7. James S, Turner W, Schwer B. How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing. RNA. 2002;8:1068-77 pubmed
    ..suggest that second step factors are recruited to the spliceosome in the following order: Slu7 --> Prp18 --> Prp22. All three proteins are released from the spliceosome after step 2 concomitant with release of mature mRNA.
  8. Vincent K, Wang Q, Jay S, Hobbs K, Rymond B. Genetic interactions with CLF1 identify additional pre-mRNA splicing factors and a link between activators of yeast vesicular transport and splicing. Genetics. 2003;164:895-907 pubmed
    ..e., clf1Delta2). The identified genes encode the Mud2, Ntc20, Prp16, Prp17, Prp19, Prp22, and Syf2 splicing factors and four proteins without established contribution to splicing (Bud13, Cet1, Cwc2, and ..
  9. Chen H, Tseng C, Tsai R, Chung C, Cheng S. Link of NTR-mediated spliceosome disassembly with DEAH-box ATPases Prp2, Prp16, and Prp22. Mol Cell Biol. 2013;33:514-25 pubmed publisher
    ..spliceosomes arrested specifically after the ATP-dependent action of DEAH-box ATPase Prp2, Prp16, or Prp22 but not at steps before the action of these ATPases or upon their binding to the spliceosome...

More Information


  1. Tanaka N, Schwer B. Characterization of the NTPase, RNA-binding, and RNA helicase activities of the DEAH-box splicing factor Prp22. Biochemistry. 2005;44:9795-803 pubmed
    The DEAH protein Prp22 is important for the second transesterification step of pre-mRNA splicing, and it is essential for releasing mature mRNA from the spliceosome...
  2. Kudlinzki D, Schmitt A, Christian H, Ficner R. Structural analysis of the C-terminal domain of the spliceosomal helicase Prp22. Biol Chem. 2012;393:1131-40 pubmed publisher
    ..The spliceosomal DEAH-box proteins Prp2, Prp16, Prp22 and Prp43 share homologous C-terminal domains (CTD)...
  3. Ben Yehuda S, Dix I, Russell C, McGarvey M, Beggs J, Kupiec M. Genetic and physical interactions between factors involved in both cell cycle progression and pre-mRNA splicing in Saccharomyces cerevisiae. Genetics. 2000;156:1503-17 pubmed
    ..We discuss the role played by these proteins in splicing and cell cycle progression. ..
  4. Brenner T, Guthrie C. Genetic analysis reveals a role for the C terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activation. Genetics. 2005;170:1063-80 pubmed
    ..We propose that GTP hydrolysis results in a rearrangement between Prp8 and the C terminus of Snu114 that leads to release of U1 and U4, thus activating the spliceosome for catalysis. ..
  5. Dosil M. Ribosome synthesis-unrelated functions of the preribosomal factor Rrp12 in cell cycle progression and the DNA damage response. Mol Cell Biol. 2011;31:2422-38 pubmed publisher
    ..I propose that the functional duality of Rrp12 may couple the control of ribosome production to the regulation of other cellular processes during cell cycle progression. ..
  6. Düring L, Thorsen M, Petersen D, Køster B, Jensen T, Holmberg S. MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation. PLoS ONE. 2012;7:e44373 pubmed publisher
    ..Genetic interactions are observed between 2 µm-MRN1 and the splicing deficient mutants snt309?, prp3, prp4, and prp22, and additional genetic analyses link MRN1, SNT309, NHP6A/B, SWI/SNF, and RSC supporting the notion of a role of ..
  7. Semlow D, Blanco M, Walter N, Staley J. Spliceosomal DEAH-Box ATPases Remodel Pre-mRNA to Activate Alternative Splice Sites. Cell. 2016;164:985-98 pubmed publisher
    ..At the catalytic stage of splicing, suboptimal splice sites are repressed by the DEAH-box ATPases Prp16 and Prp22. Here, using budding yeast, we show that these ATPases function further by enabling the spliceosome to search for ..
  8. Gahura O, Abrhámová K, Skruzny M, Valentová A, Munzarová V, Folk P, et al. Prp45 affects Prp22 partition in spliceosomal complexes and splicing efficiency of non-consensus substrates. J Cell Biochem. 2009;106:139-51 pubmed publisher
    ..alleles of NTC members SYF1, CLF1/SYF3, NTC20, and CEF1, and 2nd step splicing factors SLU7, PRP17, PRP18, and PRP22. Cwc2-associated spliceosomal complexes purified from prp45(1-169) cells showed decreased stoichiometry of Prp22, ..
  9. Masciadri B, Areces L, Carpinelli P, Foiani M, Draetta G, Fiore F. Characterization of the BUD31 gene of Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2004;320:1342-50 pubmed
    ..We propose that the observed phenotypes for bud31-null strain could be the result of defective splicing and indicate a first functional role for Bud3lp and its homologs. ..
  10. Schneider S, Campodonico E, Schwer B. Motifs IV and V in the DEAH box splicing factor Prp22 are important for RNA unwinding, and helicase-defective Prp22 mutants are suppressed by Prp8. J Biol Chem. 2004;279:8617-26 pubmed
    The yeast pre-mRNA splicing factor Prp22 is a member of the DEAH box family of nucleic acid-stimulated ATPases and RNA helicases...
  11. Burns C, Ohi R, Mehta S, O TOOLE E, Winey M, Clark T, et al. Removal of a single alpha-tubulin gene intron suppresses cell cycle arrest phenotypes of splicing factor mutations in Saccharomyces cerevisiae. Mol Cell Biol. 2002;22:801-15 pubmed
    ..Removing the TUB1 intron from two other splicing mutants that arrest at G(2)/M, prp17Delta and prp22-1 strains, permits nuclear division, but suppression of the cell cycle block is less efficient...