Gene Symbol: PRP21
Description: Prp21p
Alias: SPP91, Prp21p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Rain J, Tartakoff A, Kramer A, Legrain P. Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolution. RNA. 1996;2:535-50 pubmed
    The yeast Prp9p, Prp11p, Prp21p proteins form a multimolecular complex identified as the SF3a splicing factor in higher eukaryotes. This factor is required for the assembly of the prespliceosome...
  2. Marsolier M, Prioleau M, Sentenac A. A RNA polymerase III-based two-hybrid system to study RNA polymerase II transcriptional regulators. J Mol Biol. 1997;268:243-9 pubmed
    ..In a model system, the interacting proteins recruiting TFIIIC to DNA were PRP21 and PRP9 or PRP21 and PRP11...
  3. Chapon C, Legrain P. A novel gene, spp91-1, suppresses the splicing defect and the pre-mRNA nuclear export in the prp9-1 mutant. EMBO J. 1992;11:3279-88 pubmed
    ..One of the suppressors, spp91-1, corrects the prp9-1 growth defect through partial restoration of splicing and by a complete reversion of the ..
  4. Wells S, Ares M. Interactions between highly conserved U2 small nuclear RNA structures and Prp5p, Prp9p, Prp11p, and Prp21p proteins are required to ensure integrity of the U2 small nuclear ribonucleoprotein in Saccharomyces cerevisiae. Mol Cell Biol. 1994;14:6337-49 pubmed
    ..Three of the proteins (Prp9p, Prp11p, and Prp21p) are associated with each other and pre-mRNA in U2-dependent splicing complexes in vitro and bind specifically to ..
  5. Vaidya V, Seshadri V, Vijayraghavan U. An extragenic suppressor of prp24-1 defines genetic interaction between PRP24 and PRP21 gene products of Saccharomyces cerevisiae. Mol Gen Genet. 1996;250:267-76 pubmed
    ..temperature-sensitive extragenic suppressor, which was shown by genetic complementation analysis to be allelic to prp21-1...
  6. Wiest D, O Day C, Abelson J. In vitro studies of the Prp9.Prp11.Prp21 complex indicate a pathway for U2 small nuclear ribonucleoprotein activation. J Biol Chem. 1996;271:33268-76 pubmed
    ..In Saccharomyces cerevisiae the mRNA splicing factors, Prp9, Prp11, and Prp21, are necessary for addition of the U2 snRNP to the pre-mRNA in an early step of spliceosome assembly...
  7. Awasthi S, Palmer R, Castro M, Mobarak C, Ruby S. New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing. J Biol Chem. 2001;276:31004-15 pubmed
    ..The mutation also led to a defect in splicing and prespliceosome formation in vitro; an indication that Exo84p has a direct role in splicing. The results elucidate a surprising link between splicing and secretion. ..
  8. Yan D, Ares M. Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits. Mol Cell Biol. 1996;16:818-28 pubmed that this region collaborates with the DEAD-box protein Prp5p and the yeast SF3a subunits Prp9p, Prp11p, and Prp21p. Together, the data show that the highly conserved RNA element downstream of the branchpoint recognition sequence ..
  9. Legrain P, Chapon C. Interaction between PRP11 and SPP91 yeast splicing factors and characterization of a PRP9-PRP11-SPP91 complex. Science. 1993;262:108-10 pubmed
    ..interactions have been identified between two Saccharomyces cerevisiae yeast splicing factors, PRP9 and SPP91. Here it is demonstrated that protein-protein interactions occur between SPP91 and PRP11...

More Information


  1. Dziembowski A, Ventura A, Rutz B, Caspary F, Faux C, Halgand F, et al. Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing. EMBO J. 2004;23:4847-56 pubmed
    ..While SF3a purification revealed only the expected subunits Prp9p, Prp11p and Prp21p, yeast SF3b was found to contain only six subunits, including previously known components (Rse1p, Hsh155p, Cus1p, ..