Gene Symbol: PRP19
Description: E3 ubiquitin-protein ligase PRP19
Alias: PSO4, E3 ubiquitin-protein ligase PRP19
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Tarn W, Hsu C, Huang K, Chen H, Kao H, Lee K, et al. Functional association of essential splicing factor(s) with PRP19 in a protein complex. EMBO J. 1994;13:2421-31 pubmed
    We have previously shown that the yeast PRP19 protein is a spliceosomal component, but is not tightly associated with small nuclear RNAs...
  2. Ohi M, Gould K. Characterization of interactions among the Cef1p-Prp19p-associated splicing complex. RNA. 2002;8:798-815 pubmed
    ..Further, our data indicate that a mutation in the U-box of Prp19p disrupts these numerous protein interactions causing Cef1p degradation and Ntc instability. ..
  3. Liu Y, Chen H, Wu N, Cheng S. A novel splicing factor, Yju2, is associated with NTC and acts after Prp2 in promoting the first catalytic reaction of pre-mRNA splicing. Mol Cell Biol. 2007;27:5403-13 pubmed
    The Prp19-associated complex (NTC) is essential for pre-mRNA splicing and is associated with the spliceosome during spliceosome activation...
  4. Tsai W, Chow Y, Chen H, Huang K, Hong R, Jan S, et al. Cef1p is a component of the Prp19p-associated complex and essential for pre-mRNA splicing. J Biol Chem. 1999;274:9455-62 pubmed
  5. Chen C, Yu W, Tsao T, Wang L, Chen H, Lin J, et al. Functional and physical interactions between components of the Prp19p-associated complex. Nucleic Acids Res. 2002;30:1029-37 pubmed
    ..Genetic analysis suggests that Ntc31p, Ntc30p and Ntc20p may play roles in modulating the function of Ntc90p. ..
  6. Ohi M, Vander Kooi C, Rosenberg J, Ren L, Hirsch J, Chazin W, et al. Structural and functional analysis of essential pre-mRNA splicing factor Prp19p. Mol Cell Biol. 2005;25:451-60 pubmed
    ..These structural and functional data provide a basis for understanding the role of Prp19p as a key architectural component of the NTC. ..
  7. Chiu Y, Liu Y, Chiang T, Yeh T, Tseng C, Wu N, et al. Cwc25 is a novel splicing factor required after Prp2 and Yju2 to facilitate the first catalytic reaction. Mol Cell Biol. 2009;29:5671-8 pubmed publisher
    Cwc25 has previously been identified to associate with pre-mRNA splicing factor Cef1/Ntc85, a component of the Prp19-associated complex (nineteen complex, or NTC) involved in spliceosome activation...
  8. Yeh T, Liu H, Chung C, Wu N, Liu Y, Cheng S. Splicing factor Cwc22 is required for the function of Prp2 and for the spliceosome to escape from a futile pathway. Mol Cell Biol. 2011;31:43-53 pubmed publisher
    Cwc22 was previously identified to associate with the pre-mRNA splicing factor Cef1/Ntc85, a component of the Prp19-associated complex (nineteen complex [NTC]) involved in spliceosome activation...
  9. Chang K, Chen H, Cheng S. Ntc90 is required for recruiting first step factor Yju2 but not for spliceosome activation. RNA. 2009;15:1729-39 pubmed publisher
    The Prp19-associated complex (NineTeen Complex [NTC]) is required for spliceosome activation by specifying interactions of U5 and U6 with pre-mRNA on the spliceosome after the release of U4...

More Information


  1. Chen C, Tsai W, Chen H, Wang C, Cheng S. Identification and characterization of two novel components of the Prp19p-associated complex, Ntc30p and Ntc20p. J Biol Chem. 2001;276:488-94 pubmed
    ..These results indicate that Ntc30p and Ntc20p are auxiliary splicing factors the functions of which may be modulating the function of the Prp19p-associated complex. ..
  2. Chan S, Kao D, Tsai W, Cheng S. The Prp19p-associated complex in spliceosome activation. Science. 2003;302:279-82 pubmed
  3. Vincent K, Wang Q, Jay S, Hobbs K, Rymond B. Genetic interactions with CLF1 identify additional pre-mRNA splicing factors and a link between activators of yeast vesicular transport and splicing. Genetics. 2003;164:895-907 pubmed
    ..e., clf1Delta2). The identified genes encode the Mud2, Ntc20, Prp16, Prp17, Prp19, Prp22, and Syf2 splicing factors and four proteins without established contribution to splicing (Bud13, Cet1, ..
  4. Chen H, Tsao T, Chen C, Tsai W, Her L, Hsu M, et al. Snt309p modulates interactions of Prp19p with its associated components to stabilize the Prp19p-associated complex essential for pre-mRNA splicing. Proc Natl Acad Sci U S A. 1999;96:5406-11 pubmed
    The SNT309 gene was identified via a mutation that causes lethality of cells in combination with a prp19 mutation...
  5. Chan S, Cheng S. The Prp19-associated complex is required for specifying interactions of U5 and U6 with pre-mRNA during spliceosome activation. J Biol Chem. 2005;280:31190-9 pubmed
    ..release of U1 and U4 small nuclear ribonucleoprotein particles and the addition of a large protein complex, the Prp19-associated complex...
  6. Chen H, Jan S, Tsao T, Sheu Y, Banroques J, Cheng S. Snt309p, a component of the Prp19p-associated complex that interacts with Prp19p and associates with the spliceosome simultaneously with or immediately after dissociation of U4 in the same manner as Prp19p. Mol Cell Biol. 1998;18:2196-204 pubmed
    ..complex, we have isolated trans-acting mutations that exacerbate the phenotypes of conditional alleles of prp19, using the ade2-ade3 sectoring system. A novel splicing factor, Snt309p, was identified through such a screen...
  7. Chanarat S, Seizl M, Strasser K. The Prp19 complex is a novel transcription elongation factor required for TREX occupancy at transcribed genes. Genes Dev. 2011;25:1147-58 pubmed publisher
    ..However, it is unknown how TREX is recruited to actively transcribed genes. Here, we show that the Prp19 splicing complex functions in transcription elongation...
  8. Tsai R, Fu R, Yeh F, Tseng C, Lin Y, Huang Y, et al. Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2. Genes Dev. 2005;19:2991-3003 pubmed
    ..and Ntr2 (NineTeen complex-Related proteins) were identified for their weak association with components of the Prp19-associated complex...
  9. Sato M, Sakota M, Nakayama K. Human PRP19 interacts with prolyl-hydroxylase PHD3 and inhibits cell death in hypoxia. Exp Cell Res. 2010;316:2871-82 pubmed publisher
    ..While attempting to characterize the complex by determining its components, we identified human PRP19. hPRP19 is a multi-functional protein that plays a role in splicing, ubiquitination, and cell growth...
  10. Görnemann J, Kotovic K, Hujer K, Neugebauer K. Cotranscriptional spliceosome assembly occurs in a stepwise fashion and requires the cap binding complex. Mol Cell. 2005;19:53-63 pubmed
    ..Thus, the demonstration of an essential link between CBC and spliceosome assembly in vivo indicates that 5' end capping couples pre-mRNA splicing to transcription. ..
  11. Warkocki Z, Schneider C, Mozaffari Jovin S, Schmitzová J, Höbartner C, Fabrizio P, et al. The G-patch protein Spp2 couples the spliceosome-stimulated ATPase activity of the DEAH-box protein Prp2 to catalytic activation of the spliceosome. Genes Dev. 2015;29:94-107 pubmed publisher
    ..Our data suggest that Spp2 plays a major role in coupling Prp2's ATPase activity to remodeling of the spliceosome into a catalytically active machine. ..
  12. Khmelinskii A, Blaszczak E, Pantazopoulou M, Fischer B, Omnus D, Le Dez G, et al. Protein quality control at the inner nuclear membrane. Nature. 2014;516:410-3 pubmed publisher
    ..We show that the Asi ubiquitin ligase is involved in degradation of mislocalized integral membrane proteins, thus acting to maintain and safeguard the identity of the INM. ..
  13. Chen C, Kao D, Chan S, Kao T, Lin J, Cheng S. Functional links between the Prp19-associated complex, U4/U6 biogenesis, and spliceosome recycling. RNA. 2006;12:765-74 pubmed
    The Prp19-associated complex, consisting of at least eight protein components, is involved in spliceosome activation by specifying the interaction of U5 and U6 with pre-mRNA for their stable association with the spliceosome after U4 ..
  14. de Morais M, Vicente E, Brozmanova J, Schenberg A, Henriques J. Further characterization of the yeast pso4-1 mutant: interaction with rad51 and rad52 mutants after photoinduced psoralen lesions. Curr Genet. 1996;29:211-8 pubmed
    The pso4-1 mutant was characterized as deficient in some types of recombination, including gene conversion, crossing over, and intrachromosomal recombination...
  15. Lu P, Lu G, Yan C, Wang L, Li W, Yin P. Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition. Biochem J. 2012;441:591-7 pubmed publisher
    The Prp19-associated complex [NTC (nineteen complex)] plays a crucial role in intron removal during premature mRNA splicing in eukaryotes. Only one component of the NTC, Cwc2, is capable of binding RNA...
  16. Löscher M, Fortschegger K, Ritter G, Wostry M, Voglauer R, Schmid J, et al. Interaction of U-box E3 ligase SNEV with PSMB4, the beta7 subunit of the 20 S proteasome. Biochem J. 2005;388:593-603 pubmed
    ..Since the yeast homologue of SNEV, Prp19, also interacts with the yeast beta7 subunit of the proteasome, this mechanism seems to be conserved during ..
  17. Masciadri B, Areces L, Carpinelli P, Foiani M, Draetta G, Fiore F. Characterization of the BUD31 gene of Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2004;320:1342-50 pubmed
    ..We propose that the observed phenotypes for bud31-null strain could be the result of defective splicing and indicate a first functional role for Bud3lp and its homologs. ..
  18. Gahura O, Abrhámová K, Skruzny M, Valentová A, Munzarová V, Folk P, et al. Prp45 affects Prp22 partition in spliceosomal complexes and splicing efficiency of non-consensus substrates. J Cell Biochem. 2009;106:139-51 pubmed publisher
    ..Our data suggest that Prp45 contributes, in part through its interaction with the 2nd step-proofreading helicase Prp22, to splicing efficiency of substrates non-conforming to the consensus. ..
  19. Tarn W, Lee K, Cheng S. Yeast precursor mRNA processing protein PRP19 associates with the spliceosome concomitant with or just after dissociation of U4 small nuclear RNA. Proc Natl Acad Sci U S A. 1993;90:10821-5 pubmed
    ..We also show that the yeast precursor mRNA processing protein PRP19 becomes associated with the spliceosome during this transition...
  20. Brenner T, Guthrie C. Genetic analysis reveals a role for the C terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activation. Genetics. 2005;170:1063-80 pubmed
    ..We propose that GTP hydrolysis results in a rearrangement between Prp8 and the C terminus of Snu114 that leads to release of U1 and U4, thus activating the spliceosome for catalysis. ..
  21. Chen H, Chang K, Su Y, Huang Y, Cheng S. Structural requirement of Ntc77 for spliceosome activation and first catalytic step. Nucleic Acids Res. 2014;42:12261-71 pubmed publisher
    The Prp19-associated complex is required for spliceosome activation by stabilizing the binding of U5 and U6 on the spliceosome after the release of U4...
  22. Ohi M, Vander Kooi C, Rosenberg J, Chazin W, Gould K. Structural insights into the U-box, a domain associated with multi-ubiquitination. Nat Struct Biol. 2003;10:250-5 pubmed
    ..These comparative structural and functional analyses imply that the U-box and its associated ubiquitin ligase activity are critical for Prp19p function in vivo. ..
  23. Revers L, Cardone J, Bonatto D, Saffi J, Grey M, Feldmann H, et al. Thermoconditional modulation of the pleiotropic sensitivity phenotype by the Saccharomyces cerevisiae PRP19 mutant allele pso4-1. Nucleic Acids Res. 2002;30:4993-5003 pubmed
    The conditionally-lethal pso4-1 mutant allele of the spliceosomal-associated PRP19 gene allowed us to study this gene's influence on pre-mRNA processing, DNA repair and sporulation...
  24. Coltri P, Oliveira C. Cwc24p is a general Saccharomyces cerevisiae splicing factor required for the stable U2 snRNP binding to primary transcripts. PLoS ONE. 2012;7:e45678 pubmed publisher
    ..We propose a model in which Cwc24p is required for stabilizing the U2 association with primary transcripts, and therefore, especially important for splicing of RNAs containing non-consensus branchpoint sequences. ..
  25. Vander Kooi C, Ren L, Xu P, Ohi M, Gould K, Chazin W. The Prp19 WD40 domain contains a conserved protein interaction region essential for its function. Structure. 2010;18:584-93 pubmed publisher
    b>Prp19 is a member of the WD40 repeat family of E3 ubiquitin ligases and a conserved eukaryotic RNA splicing factor essential for activation and stabilization of the spliceosome...