Gene Symbol: PRP11
Description: Prp11p
Alias: RNA11, Prp11p
Species: Saccharomyces cerevisiae S288c
Products:     PRP11

Top Publications

  1. Yan D, Ares M. Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits. Mol Cell Biol. 1996;16:818-28 pubmed
    ..interactions show that this region collaborates with the DEAD-box protein Prp5p and the yeast SF3a subunits Prp9p, Prp11p, and Prp21p...
  2. Wiest D, O Day C, Abelson J. In vitro studies of the Prp9.Prp11.Prp21 complex indicate a pathway for U2 small nuclear ribonucleoprotein activation. J Biol Chem. 1996;271:33268-76 pubmed
    ..In Saccharomyces cerevisiae the mRNA splicing factors, Prp9, Prp11, and Prp21, are necessary for addition of the U2 snRNP to the pre-mRNA in an early step of spliceosome assembly...
  3. Abovich N, Liao X, Rosbash M. The yeast MUD2 protein: an interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition. Genes Dev. 1994;8:843-54 pubmed
    ..Moreover, experiments in the two-hybrid system show that PRP11P, a recently identified component of U2 snRNP, can interact directly with MUD2P...
  4. Legrain P, Chapon C. Interaction between PRP11 and SPP91 yeast splicing factors and characterization of a PRP9-PRP11-SPP91 complex. Science. 1993;262:108-10 pubmed
    ..Here it is demonstrated that protein-protein interactions occur between SPP91 and PRP11. The combination of the prp9-1 mutant and a truncated prp11 mutant exhibits a synthetic lethal phenotype, ..
  5. Rain J, Tartakoff A, Kramer A, Legrain P. Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolution. RNA. 1996;2:535-50 pubmed
    The yeast Prp9p, Prp11p, Prp21p proteins form a multimolecular complex identified as the SF3a splicing factor in higher eukaryotes. This factor is required for the assembly of the prespliceosome...
  6. Dziembowski A, Ventura A, Rutz B, Caspary F, Faux C, Halgand F, et al. Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing. EMBO J. 2004;23:4847-56 pubmed
    ..While SF3a purification revealed only the expected subunits Prp9p, Prp11p and Prp21p, yeast SF3b was found to contain only six subunits, including previously known components (Rse1p, ..
  7. Munding E, Shiue L, Katzman S, Donohue J, Ares M. Competition between pre-mRNAs for the splicing machinery drives global regulation of splicing. Mol Cell. 2013;51:338-48 pubmed publisher
    ..of the RPG-dedicated transcription factor gene IFH1 genetically suppresses two spliceosome mutations, prp11-1 and prp4-1, and globally restores splicing efficiency in prp4-1 cells...
  8. Wells S, Ares M. Interactions between highly conserved U2 small nuclear RNA structures and Prp5p, Prp9p, Prp11p, and Prp21p proteins are required to ensure integrity of the U2 small nuclear ribonucleoprotein in Saccharomyces cerevisiae. Mol Cell Biol. 1994;14:6337-49 pubmed
    ..Three of the proteins (Prp9p, Prp11p, and Prp21p) are associated with each other and pre-mRNA in U2-dependent splicing complexes in vitro and bind ..
  9. Yan D, Perriman R, Igel H, Howe K, Neville M, Ares M. CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif. Mol Cell Biol. 1998;18:5000-9 pubmed
    ..CUS2 protein is associated with U2 RNA in splicing extracts and interacts with PRP11, a subunit of the conserved splicing factor SF3a...

More Information


  1. Kafasla P, Barrass J, Thompson E, Fromont Racine M, Jacquier A, Beggs J, et al. Interaction of yeast eIF4G with spliceosome components: implications in pre-mRNA processing events. RNA Biol. 2009;6:563-74 pubmed
    ..In addition, Tif4631p and Tif4632p associate with protein components of the splicing machinery, namely Snu71p and Prp11p. To further delineate these interactions, we map the regions of Tif4631p and Tif4632p that are important for the ..
  2. Maddock J, Weidenhammer E, Adams C, Lunz R, Woolford J. Extragenic suppressors of Saccharomyces cerevisiae prp4 mutations identify a negative regulator of PRP genes. Genetics. 1994;136:833-47 pubmed
    ..Each of these spp mutations also suppresses prp3; spp41 and spp42 suppress prp11 as well...
  3. Wells S, Neville M, Haynes M, Wang J, Igel H, Ares M. CUS1, a suppressor of cold-sensitive U2 snRNA mutations, is a novel yeast splicing factor homologous to human SAP 145. Genes Dev. 1996;10:220-32 pubmed
    ..Extra copies of wild-type CUS1, but not CUS1-54, suppress the temperature-sensitive prp11 and prp5 mutations, linking CUS1 protein to a subset of other factors required at the same step of spliceosome ..
  4. Marsolier M, Prioleau M, Sentenac A. A RNA polymerase III-based two-hybrid system to study RNA polymerase II transcriptional regulators. J Mol Biol. 1997;268:243-9 pubmed
    ..In a model system, the interacting proteins recruiting TFIIIC to DNA were PRP21 and PRP9 or PRP21 and PRP11. Mutations affecting the interaction between PRP21 and PRP9, or PRP21 and PRP11 decreased UAS(G)-SNR6 activation ..
  5. Spingola M, Armisen J, Ares M. Mer1p is a modular splicing factor whose function depends on the conserved U2 snRNP protein Snu17p. Nucleic Acids Res. 2004;32:1242-50 pubmed
    ..Thirdly, Mer1p interacts with the branch point binding proteins Mud2p and Bbp1p and the U2 snRNP protein Prp11p by two-hybrid assays...
  6. Balzer R, Henry M. Snu56p is required for Mer1p-activated meiotic splicing. Mol Cell Biol. 2008;28:2497-508 pubmed publisher
    ..Interestingly, these two proteins do not interact, suggesting that Snu56p links pre-mRNA-bound Mer1p to Nam8p in the U1 snRNP. This work demonstrates that the Snu56 protein is required for splicing only during meiosis. ..