PPH21

Summary

Gene Symbol: PPH21
Description: Pph21p
Alias: PPH1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. pmc Intranuclear function for protein phosphatase 2A: Pph21 and Pph22 are required for rapamycin-induced GATA factor binding to the DAL5 promoter in yeast
    Isabelle Georis
    Institut de Recherches Microbiologiques J M Wiame, Laboratoire de Microbiologie, Universite Libre de Bruxelles, B1070 Brussels, Belgium
    Mol Cell Biol 31:92-104. 2011
  2. pmc Regulation of Mih1/Cdc25 by protein phosphatase 2A and casein kinase 1
    Gayatri Pal
    Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    J Cell Biol 180:931-45. 2008
  3. pmc Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B-type subunits Cdc55p and Rts1p
    H Wei
    Department of Biochemistry and Winship Cancer Center, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 276:1570-7. 2001
  4. pmc Generation of active protein phosphatase 2A is coupled to holoenzyme assembly
    Hans Hombauer
    Department of Medical Biochemistry, Max F Perutz Laboratories, Medical University of Vienna, Vienna, Austria
    PLoS Biol 5:e155. 2007
  5. pmc The Zds proteins control entry into mitosis and target protein phosphatase 2A to the Cdc25 phosphatase
    Sidonie Wicky
    Department of Molecular, Cell, and Developmental Biology, Univ of California, Santa Cruz, CA 95064, USA
    Mol Biol Cell 22:20-32. 2011
  6. pmc Zds2p regulates Swe1p-dependent polarized cell growth in Saccharomyces cerevisiae via a novel Cdc55p interaction domain
    Kimberly Yasutis
    Departments of Biology and Cell Biology, University of Virginia, Charlottesville, VA 22904, USA
    Mol Biol Cell 21:4373-86. 2010
  7. pmc Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2
    Christine Van Hoof
    Afdeling Biochemie, Faculteit Geneeskunde, Campus Gasthuisberg O and N, Katholieke Universiteit Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Biochem J 386:93-102. 2005
  8. ncbi The Saccharomyces cerevisiae phosphotyrosyl phosphatase activator proteins are required for a subset of the functions disrupted by protein phosphatase 2A mutations
    C Van Hoof
    Afdeling Biochemie, Departement Geneeskunde, Katholieke Universiteit Leuven, Herestraat 49, Leuven, B 3000, Belgium
    Exp Cell Res 264:372-87. 2001
  9. pmc Saccharomyces cerevisiae protein phosphatase 2A performs an essential cellular function and is encoded by two genes
    A A Sneddon
    Department of Biochemistry, The University, Dundee, UK
    EMBO J 9:4339-46. 1990
  10. pmc The yeast phosphotyrosyl phosphatase activator is part of the Tap42-phosphatase complexes
    Yin Zheng
    Department of Pharmacology, University of Pittsburgh School of Medicine, PA 15213, USA
    Mol Biol Cell 16:2119-27. 2005

Scientific Experts

  • Yanchang Wang
  • Jennifer J Tate
  • Isabelle Georis
  • Yu Jiang
  • Evelyne Dubois
  • Terrance G Cooper
  • Huamin Wang
  • Paula McCourt
  • Tammy Pracheil
  • Dries Castermans
  • Andrew J Oler
  • Yidi Sun
  • Valentina Rossio
  • Sidonie Wicky
  • Kimberly Yasutis
  • André Feller
  • Douglas R Kellogg
  • Leon Y Chan
  • Veerle Janssens
  • Gayatri Pal
  • Akio Nakashima
  • Hans Hombauer
  • Lars Kuepfer
  • Egon Ogris
  • Ingrid Mudrak
  • Gonghong Yan
  • Christian G Riedel
  • Christine Van Hoof
  • Yin Zheng
  • H Yang
  • Ivan Munoz
  • Naoko Sakumoto
  • Christina Gallo-Ebert
  • Joseph T Nickels
  • Yan Gonghong
  • Hiromu Takematsu
  • Yansong Miao
  • Yukari Yamane
  • Zhengchang Liu
  • Yasunori Kozutsumi
  • Kevan M Shokat
  • Janet Thornton
  • C Van Hoof
  • Wendy Louwet
  • D A Mitchell
  • Matthias Versele
  • Johan M Thevelein
  • H Wei
  • Bradley R Cairns
  • Johan Kriel
  • Stefaan Wera
  • Ils Somers
  • David G Drubin
  • Chao Zhang
  • David Schieltz
  • Hendri Tjandra
  • John Yates
  • Satoshi Yoshida
  • Feven Tameire
  • Matthew Ryder
  • Stanley Fields
  • Keith G Kozminski
  • Shubha A Dighe
  • Marissa Vignali
  • Angelika Amon
  • Yoshiaki Kamada
  • Ushio Kikkawa
  • Ippei Kawanishi
  • Kazuyoshi Yonezawa
  • Hideki Takata
  • Akira Matsuura
  • Yoshiko Maruki
  • Maria T Z Paraz
  • Yoshinori Ohsumi
  • Tomoko Kawamata
  • Kyung S Lee
  • Y Jiang
  • Chika Kondo
  • Yuko Imamura
  • Matthias Peter
  • Thomas Fellner
  • Uwe Sauer
  • Daniel H Lackner
  • David Weismann
  • Claudia Stanzel
  • Jörg Stelling
  • Katsuhiko Shirahige
  • Yuki Katou
  • Marta Galova
  • Frank Buchholz

Detail Information

Publications37

  1. pmc Intranuclear function for protein phosphatase 2A: Pph21 and Pph22 are required for rapamycin-induced GATA factor binding to the DAL5 promoter in yeast
    Isabelle Georis
    Institut de Recherches Microbiologiques J M Wiame, Laboratoire de Microbiologie, Universite Libre de Bruxelles, B1070 Brussels, Belgium
    Mol Cell Biol 31:92-104. 2011
    Protein phosphatase 2A (PP2A), a central Tor pathway phosphatase consisting of a catalytic subunit (Pph21 or Pph22), a scaffold subunit (Tpd3), and one of two regulatory subunits (Cdc55 or Rts1), has been repeatedly shown to play ..
  2. pmc Regulation of Mih1/Cdc25 by protein phosphatase 2A and casein kinase 1
    Gayatri Pal
    Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    J Cell Biol 180:931-45. 2008
    ..Because casein kinase 1 is associated with sites of polar growth, it may regulate Mih1 as part of a signaling mechanism that links successful completion of growth-related events to cell cycle progression...
  3. pmc Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B-type subunits Cdc55p and Rts1p
    H Wei
    Department of Biochemistry and Winship Cancer Center, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 276:1570-7. 2001
    ..Taken together, our results indicate that methylation and phosphorylation may be mechanisms by which the cell dynamically regulates PP2A complex formation and function...
  4. pmc Generation of active protein phosphatase 2A is coupled to holoenzyme assembly
    Hans Hombauer
    Department of Medical Biochemistry, Max F Perutz Laboratories, Medical University of Vienna, Vienna, Austria
    PLoS Biol 5:e155. 2007
    ..We propose a novel model of PP2A biogenesis in which a tightly controlled activation cascade protects cells from untargeted activity of the free catalytic PP2A subunit...
  5. pmc The Zds proteins control entry into mitosis and target protein phosphatase 2A to the Cdc25 phosphatase
    Sidonie Wicky
    Department of Molecular, Cell, and Developmental Biology, Univ of California, Santa Cruz, CA 95064, USA
    Mol Biol Cell 22:20-32. 2011
    ..Together, these observations define a role for the Zds proteins in controlling specific functions of PP2A(Cdc55) and suggest that upstream signals that regulate PP2A(Cdc55) may play an important role in controlling entry into mitosis...
  6. pmc Zds2p regulates Swe1p-dependent polarized cell growth in Saccharomyces cerevisiae via a novel Cdc55p interaction domain
    Kimberly Yasutis
    Departments of Biology and Cell Biology, University of Virginia, Charlottesville, VA 22904, USA
    Mol Biol Cell 21:4373-86. 2010
    ..This negative regulation requires the CDC55 gene. Together these data indicate that the Cdc55p/Zds2p module has a function in the regulation of the Swe1p-dependent G2/M checkpoint...
  7. pmc Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2
    Christine Van Hoof
    Afdeling Biochemie, Faculteit Geneeskunde, Campus Gasthuisberg O and N, Katholieke Universiteit Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Biochem J 386:93-102. 2005
    ..Ypa1 interacts specifically with Pph3, Sit4 and Ppg1, whereas Ypa2 binds to Pph21 and Pph22...
  8. ncbi The Saccharomyces cerevisiae phosphotyrosyl phosphatase activator proteins are required for a subset of the functions disrupted by protein phosphatase 2A mutations
    C Van Hoof
    Afdeling Biochemie, Departement Geneeskunde, Katholieke Universiteit Leuven, Herestraat 49, Leuven, B 3000, Belgium
    Exp Cell Res 264:372-87. 2001
    ..Therefore, Ypa does not seem to play a role in the regulation of PP2A at this stage of the cell cycle. These results imply that Ypa regulates a specific subset of PP2A functions, possibly by controlling the subunit composition of PP2A...
  9. pmc Saccharomyces cerevisiae protein phosphatase 2A performs an essential cellular function and is encoded by two genes
    A A Sneddon
    Department of Biochemistry, The University, Dundee, UK
    EMBO J 9:4339-46. 1990
    Two genes (PPH21 and PPH22) encoding the yeast homologues of protein serine-threonine phosphatase 2A have been cloned from a Saccharomyces cerevisiae genomic library using a rabbit protein phosphatase 2A cDNA as a hybridization probe...
  10. pmc The yeast phosphotyrosyl phosphatase activator is part of the Tap42-phosphatase complexes
    Yin Zheng
    Department of Pharmacology, University of Pittsburgh School of Medicine, PA 15213, USA
    Mol Biol Cell 16:2119-27. 2005
    ....
  11. pmc Cdc55p, the B-type regulatory subunit of protein phosphatase 2A, has multiple functions in mitosis and is required for the kinetochore/spindle checkpoint in Saccharomyces cerevisiae
    Y Wang
    Department of Biology, University of Virginia, Charlottesville 22903, USA
    Mol Cell Biol 17:620-6. 1997
    ..Finally, cdc55::LEU2 suppresses the temperature sensitivity of cdc20-1, suggesting additional roles for CDC55 in mitosis...
  12. pmc Loss of a protein phosphatase 2A regulatory subunit (Cdc55p) elicits improper regulation of Swe1p degradation
    H Yang
    Department of Biology, Syracuse University, Syracuse, New York 13244, USA
    Mol Cell Biol 20:8143-56. 2000
    ..We also present evidence indicating that, in cdc55-null cells, misregulated PP2A phosphatase activity is the cause of both the ectopic stabilization of Swe1p and the production of the morphologically abnormal phenotype...
  13. pmc Tor proteins and protein phosphatase 2A reciprocally regulate Tap42 in controlling cell growth in yeast
    Y Jiang
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    EMBO J 18:2782-92. 1999
    ..of a protein, Tap42, with two different protein phosphatase catalytic subunits; one encoded redundantly by PPH21 and PPH22, and one encoded by SIT4...
  14. pmc Molecular genetic analysis of Rts1p, a B' regulatory subunit of Saccharomyces cerevisiae protein phosphatase 2A
    Y Shu
    Department of Biology, Syracuse University, New York 13244, USA
    Mol Cell Biol 17:3242-53. 1997
    ..Genetic analyses show that the role played by Rts1p in PP2A regulation is distinctly different from that played by the other known variable B regulatory subunit, Cdc55p, a protein recently implicated in checkpoint control regulation...
  15. pmc Phosphatase 2A negatively regulates mitotic exit in Saccharomyces cerevisiae
    Yanchang Wang
    Department of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, FL 32306, USA
    Mol Biol Cell 17:80-9. 2006
    ..Genes that encode the B-regulatory subunit (Cdc55) and the three catalytic subunits (Pph21, Pph22, and Pph3) of phosphatase 2A (PP2A) were isolated...
  16. pmc Protein phosphatase 2A in Saccharomyces cerevisiae: effects on cell growth and bud morphogenesis
    H Ronne
    Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden
    Mol Cell Biol 11:4876-84. 1991
    We have cloned three genes for protein phosphatases in the yeast Saccharomyces cerevisiae. Two of the genes, PPH21 and PPH22, encode highly similar proteins that are homologs of the mammalian protein phosphatase 2A (PP2A), while the ..
  17. pmc Interaction with Tap42 is required for the essential function of Sit4 and type 2A phosphatases
    Huamin Wang
    Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Mol Biol Cell 14:4342-51. 2003
    In Saccharomyces cerevisiae, Pph21 and Pph22 are the two catalytic subunits of type 2A phosphatase (PP2Ac), and Sit4 is a major form of 2A-like phosphatase...
  18. pmc The Tap42-protein phosphatase type 2A catalytic subunit complex is required for cell cycle-dependent distribution of actin in yeast
    Huamin Wang
    Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Mol Cell Biol 23:3116-25. 2003
    ..In addition, we provide evidence that PP2A activity plays a negative role in controlling the actin cytoskeleton and, possibly, in regulation of the G(2)/M transition of the cell cycle...
  19. pmc The role of Saccharomyces cerevisiae type 2A phosphatase in the actin cytoskeleton and in entry into mitosis
    F C Lin
    Cold Spring Harbor Laboratory, NY 11724, USA
    EMBO J 14:2745-59. 1995
    We have prepared a temperature-sensitive Saccharomyces cerevisiae type 2A phosphatase (PP2A) mutant, pph21-102...
  20. pmc Orm protein phosphoregulation mediates transient sphingolipid biosynthesis response to heat stress via the Pkh-Ypk and Cdc55-PP2A pathways
    Yidi Sun
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Biol Cell 23:2388-98. 2012
    ..We propose that this mechanism serves as the basis for how Orm phosphoregulation controls sphingolipid biosynthesis in response to stress in a kinetically coupled manner...
  21. pmc PP4 dephosphorylates Maf1 to couple multiple stress conditions to RNA polymerase III repression
    Andrew J Oler
    HHMI, Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT, USA
    EMBO J 31:1440-52. 2012
    ....
  22. pmc TORC2 signaling is antagonized by protein phosphatase 2A and the Far complex in Saccharomyces cerevisiae
    Tammy Pracheil
    Department of Biological Sciences, University of New Orleans, New Orleans, Louisiana 70148, USA
    Genetics 190:1325-39. 2012
    ..a member of the Far3-7-8-9-10-11 complex involved in pheromone-induced cell cycle arrest, interacts with Tpd3 and Pph21, conserved components of PP2A, and deletions of components of the Far3-7-8-9-10-11 complex and PP2A rescue growth ..
  23. pmc Glucose-induced posttranslational activation of protein phosphatases PP2A and PP1 in yeast
    Dries Castermans
    Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KULeuven, Belgium
    Cell Res 22:1058-77. 2012
    ..Rts1 interacted with both Pph21 and Glc7 under different conditions and these interactions were Reg1 dependent...
  24. pmc PP2A(Cdc55) regulates G1 cyclin stability
    Paula McCourt
    Venenum Biodesign, Genesis Biotechnology Group, Hamilton, NJ, USA
    Cell Cycle 12:1201-10. 2013
    ..In sum, our results indicate that PP2A is absolutely required to maintain G1-S cyclin levels through modulating their phosphorylation status, an event necessary to properly transit through the cell cycle...
  25. pmc Rapamycin activates Tap42-associated phosphatases by abrogating their association with Tor complex 1
    Gonghong Yan
    Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    EMBO J 25:3546-55. 2006
    ..In addition, our data support a model in which rapamycin acts not by inhibiting the kinase activity of Tor but by disrupting its interaction with downstream targets...
  26. ncbi Overexpression of yeast PAM1 gene permits survival without protein phosphatase 2A and induces a filamentous phenotype
    G Z Hu
    Ludwig Institute for Cancer Research, Uppsala Biomedical Center, Sweden
    J Biol Chem 269:3429-35. 1994
    ..In the yeast Saccharomyces cerevisiae, the catalytic subunit of PP2A is encoded by two duplicated genes, PPH21 and PPH22. A third related gene, PPH3, also contributes some PP2A activity...
  27. ncbi Identification of multicopy suppressors of cell cycle arrest at the G1-S transition in Saccharomyces cerevisiae
    Ivan Munoz
    Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Bellaterra 08193, Barcelona
    Yeast 20:157-69. 2003
    ..The possible relationship between the known functions of these suppressor genes and the progress through the G(1)-S transition is discussed...
  28. ncbi A series of double disruptants for protein phosphatase genes in Saccharomyces cerevisiae and their phenotypic analysis
    Naoko Sakumoto
    Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Yeast 19:587-99. 2002
    ..Four double disruptants exhibited synthetic phenotypes in addition to eight single ones: the pph21 pph22 double disruptant showed slow growth on complete medium, as did the sit4 and yvh1 single ones...
  29. ncbi Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I
    Christian G Riedel
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Nature 441:53-61. 2006
    ..Our data are consistent with the notion that efficient cleavage of Rec8 requires phosphorylation of cohesin and that this is blocked by PP2A at meiosis I centromeres...
  30. pmc Spatial regulation of Cdc55-PP2A by Zds1/Zds2 controls mitotic entry and mitotic exit in budding yeast
    Valentina Rossio
    Department of Biology, Brandeis University, Waltham, MA 02454, USA
    J Cell Biol 193:445-54. 2011
    ..Our analysis defines the long-sought molecular function for the zillion different screens family proteins and reveals the importance of the regulation of PP2A localization for proper mitotic progression...
  31. ncbi Role of protein phosphatase 2A in the control of glycogen metabolism in yeast
    J Clotet
    Departament de Bioquimica i Biologia Molecular, Facultat de Veterinaria, Universitat Autonoma de Barcelona, Spain
    Eur J Biochem 229:207-14. 1995
    The yeast homologues of mammalian protein phosphatase 2A (PP2A) are encoded by two genes, PPH21 and PPH22...
  32. pmc The phosphotyrosyl phosphatase activator, Ncs1p (Rrd1p), functions with Cla4p to regulate the G(2)/M transition in Saccharomyces cerevisiae
    D A Mitchell
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Mol Cell Biol 21:488-500. 2001
    ..Strains lacking both NCS1 and NOH1 were inviable and arrested as unbudded cells, implying that PTPA function is required for proper G(1) progression...
  33. pmc Distinct phosphatase requirements and GATA factor responses to nitrogen catabolite repression and rapamycin treatment in Saccharomyces cerevisiae
    Jennifer J Tate
    Department of Molecular Sciences, University of Tennessee, Memphis, Tennessee 38163, USA
    J Biol Chem 285:17880-95. 2010
    ..We previously demonstrated that the Sit4 and Pph21/22-Tpd3-Cdc55/Rts1 requirements for nuclear Gln3 localization differ...
  34. ncbi Ensemble modeling for analysis of cell signaling dynamics
    Lars Kuepfer
    Institute of Molecular Systems Biology, ETH Zurich, 8092 Zurich, Switzerland
    Nat Biotechnol 25:1001-6. 2007
    ..Beyond revising our picture of TOR signaling, we expect ensemble modeling to help elucidate other insufficiently characterized cellular circuits...
  35. pmc Rapamycin-induced Gln3 dephosphorylation is insufficient for nuclear localization: Sit4 and PP2A phosphatases are regulated and function differently
    Jennifer J Tate
    Department of Molecular Sciences, University of Tennessee, Memphis, Tennessee 38163, USA
    J Biol Chem 284:2522-34. 2009
    ..In contrast, Rap-induced nuclear Gln3 localization absolutely requires all four PP2A components (Pph21/22, Tpd3, Cdc55, and Rts1)...
  36. pmc The yeast Tor signaling pathway is involved in G2/M transition via polo-kinase
    Akio Nakashima
    Biosignal Research Center, Kobe University, Kobe, Japan
    PLoS ONE 3:e2223. 2008
    ..The C-terminal polo-box domain of Cdc5 has an inhibitory role in nuclear translocation. Taken together, our results indicate a novel function of Tor in the regulation of cell cycle and proliferation...
  37. pmc The protein phosphatase 2A functions in the spindle position checkpoint by regulating the checkpoint kinase Kin4
    Leon Y Chan
    David H Koch Institute for Integrative Cancer Research and Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 23:1639-49. 2009
    ....