Gene Symbol: POT1
Description: acetyl-CoA C-acyltransferase
Alias: FOX3, POX3, acetyl-CoA C-acyltransferase
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Erdmann R, Kunau W. Purification and immunolocalization of the peroxisomal 3-oxoacyl-CoA thiolase from Saccharomyces cerevisiae. Yeast. 1994;10:1173-82 pubmed
    ..We also show that these antibodies could be suitable for an immunofluorescence microscopy screening of yeast mutants affected in peroxisome assembly. ..
  2. Rehling P, Marzioch M, Niesen F, Wittke E, Veenhuis M, Kunau W. The import receptor for the peroxisomal targeting signal 2 (PTS2) in Saccharomyces cerevisiae is encoded by the PAS7 gene. EMBO J. 1996;15:2901-13 pubmed
  3. Albertini M, Rehling P, Erdmann R, Girzalsky W, Kiel J, Veenhuis M, et al. Pex14p, a peroxisomal membrane protein binding both receptors of the two PTS-dependent import pathways. Cell. 1997;89:83-92 pubmed
    ..The data presented here are consistent with the idea of a common translocation machinery for both PTS-dependent protein import pathways in the peroxisomal membrane...
  4. Glover J, Andrews D, Rachubinski R. Saccharomyces cerevisiae peroxisomal thiolase is imported as a dimer. Proc Natl Acad Sci U S A. 1994;91:10541-5 pubmed
    ..This observation suggests that interactions between thiolase subunits are not disrupted during translocation. ..
  5. Scherrer T, Mittal N, Janga S, Gerber A. A screen for RNA-binding proteins in yeast indicates dual functions for many enzymes. PLoS ONE. 2010;5:e15499 pubmed publisher
    ..Our results suggest that many proteins may associate with mRNAs and possibly control their fates, providing dense connections between different layers of cellular regulation. ..
  6. Mathieu M, Zeelen J, Pauptit R, Erdmann R, Kunau W, Wierenga R. The 2.8 A crystal structure of peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: a five-layered alpha beta alpha beta alpha structure constructed from two core domains of identical topology. Structure. 1994;2:797-808 pubmed
    ..The two active sites are on the same face of the dimer, far from the amino and carboxyl termini of both subunits and the disordered amino-terminal import signal sequence. ..
  7. Montilla Martinez M, Beck S, Klümper J, Meinecke M, Schliebs W, Wagner R, et al. Distinct Pores for Peroxisomal Import of PTS1 and PTS2 Proteins. Cell Rep. 2015;13:2126-34 pubmed publisher
    ..Our results demonstrate that import of PTS1 and PTS2 proteins does not converge at the peroxisomal membrane as previously anticipated but is performed by distinct pores. ..
  8. Pan D, Nakatsu T, Kato H. Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Nat Struct Mol Biol. 2013;20:987-93 pubmed publisher
    ..Together with in vivo and in vitro functional assays of Pex7p and Pex21p variants, our findings reveal the molecular mechanism of PTS2 recognition...
  9. Lefevre S, Van Roermund C, Wanders R, Veenhuis M, van der Klei I. The significance of peroxisome function in chronological aging of Saccharomyces cerevisiae. Aging Cell. 2013;12:784-93 pubmed publisher

More Information


  1. Vögtle F, Burkhart J, Rao S, Gerbeth C, Hinrichs J, Martinou J, et al. Intermembrane space proteome of yeast mitochondria. Mol Cell Proteomics. 2012;11:1840-52 pubmed publisher
    ..We present here the first and comprehensive proteome of IMS proteins of yeast mitochondria with 51 proteins in total. The IMS proteome will serve as a valuable source for further studies on the role of the IMS in cell life and death. ..
  2. Lee J, Kim S, Chae H, Jung J, Lee S. Antifungal activity of Saccharomyces cerevisiae peroxisomal 3-ketoacyl-CoA thiolase. BMB Rep. 2009;42:281-5 pubmed
    ..Yeast ScFox3 is a natural antifungal agent found in peroxisomes. ..
  3. Grunau S, Schliebs W, Linnepe R, Neufeld C, Cizmowski C, Reinartz B, et al. Peroxisomal targeting of PTS2 pre-import complexes in the yeast Saccharomyces cerevisiae. Traffic. 2009;10:451-60 pubmed publisher
  4. Marchesini S, Poirier Y. Futile cycling of intermediates of fatty acid biosynthesis toward peroxisomal beta-oxidation in Saccharomyces cerevisiae. J Biol Chem. 2003;278:32596-601 pubmed
    ..and 3-hydroxydodecanoate was synthesized in a mutant deficient in the peroxisomal 3-ketothiolase (fox3 Delta 0) growing either on oleic acid or heptadecenoic acid as well as in wild type and fox3 Delta 0 mutants grown ..
  5. Stein K, Schell Steven A, Erdmann R, Rottensteiner H. Interactions of Pex7p and Pex18p/Pex21p with the peroxisomal docking machinery: implications for the first steps in PTS2 protein import. Mol Cell Biol. 2002;22:6056-69 pubmed
    ..This complex accumulated in docking mutants but was absent in cells lacking Pex18p/Pex21p, indicating that Pex18p/Pex21p are required already before the docking event. ..
  6. Glover J, Andrews D, Subramani S, Rachubinski R. Mutagenesis of the amino targeting signal of Saccharomyces cerevisiae 3-ketoacyl-CoA thiolase reveals conserved amino acids required for import into peroxisomes in vivo. J Biol Chem. 1994;269:7558-63 pubmed
    ..cerevisiae thiolase to peroxisomes. ..
  7. Girzalsky W, Rehling P, Stein K, Kipper J, Blank L, Kunau W, et al. Involvement of Pex13p in Pex14p localization and peroxisomal targeting signal 2-dependent protein import into peroxisomes. J Cell Biol. 1999;144:1151-62 pubmed
    ..We also found Pex13p to be required for peroxisomal association of Pex14p, yet the SH3 domain of Pex13p may not provide the only binding site for Pex14p at the peroxisomal membrane...
  8. Mathieu M, Modis Y, Zeelen J, Engel C, Abagyan R, Ahlberg A, et al. The 1.8 A crystal structure of the dimeric peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: implications for substrate binding and reaction mechanism. J Mol Biol. 1997;273:714-28 pubmed
    ..The three phosphate groups of the CoA moiety are predicted to interact with side-chains of lysine and arginine residues, which are conserved in the dimeric thiolases. ..
  9. Chan A, Schummer A, Fischer S, Schröter T, Cruz Zaragoza L, Bender J, et al. Pex17p-dependent assembly of Pex14p/Dyn2p-subcomplexes of the peroxisomal protein import machinery. Eur J Cell Biol. 2016;95:585-597 pubmed publisher
    ..As dyn2? cells also display reduced peroxisomal function, our data indicate that Dyn2p-dependent formation of the high molecular weight Pex14p-complex is required to maintain peroxisomal function on wild-type level. ..