POR1

Summary

Gene Symbol: POR1
Description: porin POR1
Alias: OMP2, porin POR1
Species: Saccharomyces cerevisiae S288c
Products:     POR1

Top Publications

  1. Lee A, Xu X, Blachly Dyson E, Forte M, Colombini M. The role of yeast VDAC genes on the permeability of the mitochondrial outer membrane. J Membr Biol. 1998;161:173-81 pubmed
    In addition to the POR1 gene, which encodes the well-characterized voltage dependent anion-selective channel (YVDAC1) of the mitochondrial outer membrane, the yeast Saccharomyces cerevisiae contains a second gene (POR2) encoding a protein ..
  2. Tatsuta T, Model K, Langer T. Formation of membrane-bound ring complexes by prohibitins in mitochondria. Mol Biol Cell. 2005;16:248-59 pubmed
    ..Implications of these findings for proposed cellular activities of prohibitins are discussed. ..
  3. Chan N, Lithgow T. The peripheral membrane subunits of the SAM complex function codependently in mitochondrial outer membrane biogenesis. Mol Biol Cell. 2008;19:126-36 pubmed
    ..Very different environments surround bacteria and mitochondria, and we discuss the role of Sam35 and Sam37 in terms of the problems peculiar to mitochondrial protein substrates. ..
  4. Dietmeier K, Hönlinger A, Bömer U, Dekker P, Eckerskorn C, Lottspeich F, et al. Tom5 functionally links mitochondrial preprotein receptors to the general import pore. Nature. 1997;388:195-200 pubmed
    ..We conclude that Tom5 represents a functional link between surface receptors and GIP, and is part of an 'acid chain' that guides the stepwise transport of positively charged mitochondrial targeting sequences. ..
  5. Blachly Dyson E, Song J, Wolfgang W, Colombini M, Forte M. Multicopy suppressors of phenotypes resulting from the absence of yeast VDAC encode a VDAC-like protein. Mol Cell Biol. 1997;17:5727-38 pubmed
    ..organisms, the yeast Saccharomyces cerevisiae has been thought to contain a single VDAC gene, designated POR1. However, cells missing the POR1 gene (delta por1) were able to grow on yeast media containing a nonfermentable ..
  6. Krimmer T, Rapaport D, Ryan M, Meisinger C, Kassenbrock C, Blachly Dyson E, et al. Biogenesis of porin of the outer mitochondrial membrane involves an import pathway via receptors and the general import pore of the TOM complex. J Cell Biol. 2001;152:289-300 pubmed
    ..We conclude that import of porin does not proceed through the action of Tom20 alone, but requires an intact outer membrane and involves at least four more subunits of the TOM machinery, including the general import pore. ..
  7. Kanprasoet W, Jensen L, Sriprach S, Thitiananpakorn K, Rattanapornsompong K, Jensen A. Deletion of Mitochondrial Porin Alleviates Stress Sensitivity in the Yeast Model of Shwachman-Diamond Syndrome. J Genet Genomics. 2015;42:671-84 pubmed publisher
    ..Prior disruption of POR1, encoding the mitochondrial voltage dependent anion channel (VDAC), abrogated the effects of SDO1 deletion and ..
  8. Lauffer S, Mäbert K, Czupalla C, Pursche T, Hoflack B, Rödel G, et al. Saccharomyces cerevisiae porin pore forms complexes with mitochondrial outer membrane proteins Om14p and Om45p. J Biol Chem. 2012;287:17447-58 pubmed publisher
    ..We propose a model for the role of the two OM proteins in addressing the porin pore to bind to specific channels in the mt IM to facilitate transport of metabolites. ..
  9. Burri L, Vascotto K, Gentle I, Chan N, Beilharz T, Stapleton D, et al. Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae. FEBS J. 2006;273:1507-15 pubmed
    ..The alpha-helical outer membrane proteins provide functions derived after the endosymbiotic event, and require the translocase in the outer mitochondrial membrane complex for insertion into the outer membrane. ..

More Information

Publications33

  1. Smith M, Petrak M, Boucher P, Barton K, Carter L, Reddy G, et al. Lysine residues at positions 234 and 236 in yeast porin are involved in its assembly into the mitochondrial outer membrane. J Biol Chem. 1995;270:28331-6 pubmed
  2. Galganska H, Budzinska M, Wojtkowska M, Kmita H. Redox regulation of protein expression in Saccharomyces cerevisiae mitochondria: possible role of VDAC. Arch Biochem Biophys. 2008;479:39-45 pubmed publisher
    ..Thus, VDAC can be regarded as a participant of signaling pathways in S. cerevisiae cells. ..
  3. Wenz L, Ellenrieder L, Qiu J, Bohnert M, Zufall N, van der Laan M, et al. Sam37 is crucial for formation of the mitochondrial TOM-SAM supercomplex, thereby promoting β-barrel biogenesis. J Cell Biol. 2015;210:1047-54 pubmed publisher
    ..Sam37 thus promotes efficient transfer of β-barrel precursors to the SAM complex. We conclude that Sam37 functions as a coupling factor of the translocase supercomplex of the mitochondrial outer membrane. ..
  4. Merklinger E, Gofman Y, Kedrov A, Driessen A, Ben Tal N, Shai Y, et al. Membrane integration of a mitochondrial signal-anchored protein does not require additional proteinaceous factors. Biochem J. 2012;442:381-9 pubmed publisher
    ..Collectively, our results suggest that SA proteins are specifically inserted into the MOM by a process that is not dependent on additional proteins, but is rather facilitated by the distinct lipid composition of this membrane. ..
  5. Chang L, Lee F. The RNA helicase Dhh1p cooperates with Rbp1p to promote porin mRNA decay via its non-conserved C-terminal domain. Nucleic Acids Res. 2012;40:1331-44 pubmed publisher
    ..An RNA-binding protein, Rbp1p, is known to bind to the 3'-UTR of porin (POR1) mRNA, and induces mRNA decay by an uncharacterized mechanism...
  6. Weber Lotfi F, Ibrahim N, Boesch P, Cosset A, Konstantinov Y, Lightowlers R, et al. Developing a genetic approach to investigate the mechanism of mitochondrial competence for DNA import. Biochim Biophys Acta. 2009;1787:320-7 pubmed publisher
    ..cerevisiae strains deleted for the VDAC-1 or VDAC-2 gene are severely compromised in DNA import. The prospect is now open to screen further mutant yeast strains to identify the elusive inner membrane DNA transporter. ..
  7. Pereira C, Camougrand N, Manon S, Sousa M, Corte Real M. ADP/ATP carrier is required for mitochondrial outer membrane permeabilization and cytochrome c release in yeast apoptosis. Mol Microbiol. 2007;66:571-82 pubmed
    ..While deletion of POR1 (yeast voltage-dependent anion channel) enhances apoptosis triggered by acetic acid, H(2)O(2) and diamide, CPR3 (..
  8. Koppel D, Kinnally K, Masters P, Forte M, Blachly Dyson E, Mannella C. Bacterial expression and characterization of the mitochondrial outer membrane channel. Effects of n-terminal modifications. J Biol Chem. 1998;273:13794-800 pubmed
    ..coli. The truncated protein has a CD spectrum similar to that of mitochondrial scVDAC, but its channel activity is abnormal, exhibiting an unstable open state and rapid transitions between multiple subconductance levels. ..
  9. Brandina I, Graham J, Lemaitre Guillier C, Entelis N, Krasheninnikov I, Sweetlove L, et al. Enolase takes part in a macromolecular complex associated to mitochondria in yeast. Biochim Biophys Acta. 2006;1757:1217-28 pubmed
    ..This suggests an unsuspected novel function for this complex in tRNA mitochondrial import. ..
  10. Chacinska A, Rehling P, Guiard B, Frazier A, Schulze Specking A, Pfanner N, et al. Mitochondrial translocation contact sites: separation of dynamic and stabilizing elements in formation of a TOM-TIM-preprotein supercomplex. EMBO J. 2003;22:5370-81 pubmed
    ..Thus, Tim50 functions as a dynamic factor and the IMS domain of Tom22 represents a stabilizing element in formation of a productive translocation contact site supercomplex. ..
  11. Butcher R, Schreiber S. A small molecule suppressor of FK506 that targets the mitochondria and modulates ionic balance in Saccharomyces cerevisiae. Chem Biol. 2003;10:521-31 pubmed
    ..Our data link ionic balance to mitochondrial function and suggest a role for calcineurin in mediating this signaling network. ..
  12. Sánchez N, Pearce D, Cardillo T, Uribe S, Sherman F. Requirements of Cyc2p and the porin, Por1p, for ionic stability and mitochondrial integrity in Saccharomyces cerevisiae. Arch Biochem Biophys. 2001;392:326-32 pubmed
    ..We report that por1-Delta strains lacking the mitochondrial porin, Por1p, but not por2-Delta strains lacking the related porin, share ..
  13. Buttner S, Eisenberg T, Carmona Gutierrez D, Ruli D, Knauer H, Ruckenstuhl C, et al. Endonuclease G regulates budding yeast life and death. Mol Cell. 2007;25:233-46 pubmed
    ..Deletion of NUC1 diminishes apoptotic death when mitochondrial respiration is increased but enhances necrotic death when oxidative phosphorylation is repressed, pointing to dual--lethal and vital--roles for EndoG. ..
  14. McDonald B, Wydro M, Lightowlers R, Lakey J. Probing the orientation of yeast VDAC1 in vivo. FEBS Lett. 2009;583:739-42 pubmed publisher
    ..Two epitopes suggest that VDAC (scVDAC) has its C-terminus exposed to the cytoplasm whilst two others are more equivocal and, when combined with published data, suggest a dynamic behavior. ..
  15. Schmidt O, Harbauer A, Rao S, Eyrich B, Zahedi R, Stojanovski D, et al. Regulation of mitochondrial protein import by cytosolic kinases. Cell. 2011;144:227-39 pubmed publisher
    ..We conclude that cytosolic kinases exert stimulatory and inhibitory effects on biogenesis and function of the TOM complex and thus regulate protein import into mitochondria. ..
  16. Buttner S, Ruli D, Vögtle F, Galluzzi L, Moitzi B, Eisenberg T, et al. A yeast BH3-only protein mediates the mitochondrial pathway of apoptosis. EMBO J. 2011;30:2779-92 pubmed publisher
    ..Thus, the yeast genome encodes a functional BH3 domain that induces cell death through phylogenetically conserved mechanisms. ..
  17. Pereira H, Azevedo F, Rego A, Sousa M, Chaves S, Corte Real M. The protective role of yeast cathepsin D in acetic acid-induced apoptosis depends on ANT (Aac2p) but not on the voltage-dependent channel (Por1p). FEBS Lett. 2013;587:200-5 pubmed publisher
    ..Our results demonstrate a differential interplay between yeast vacuolar CatD and mitochondrial proteins involved in apoptosis regulation. ..
  18. Wenz L, Opaliński L, Schuler M, Ellenrieder L, Ieva R, Böttinger L, et al. The presequence pathway is involved in protein sorting to the mitochondrial outer membrane. EMBO Rep. 2014;15:678-85 pubmed publisher
    ..Assembly of Om45 in the outer membrane involves the MIM machinery. Om45 thus follows a new mitochondrial biogenesis pathway that uses elements of the presequence import pathway to direct a protein to the outer membrane. ..
  19. Murai M, Okuda A, Yamamoto T, Shinohara Y, Miyoshi H. Synthetic Ubiquinones Specifically Bind to Mitochondrial Voltage-Dependent Anion Channel 1 (VDAC1) in Saccharomyces cerevisiae Mitochondria. Biochemistry. 2017;56:570-581 pubmed publisher
    ..Thus, the results of the present study provided, for the first time to our knowledge, direct evidence indicating that the ubiquinone molecule specifically binds to VDAC1 through its quinone-head ring. ..
  20. Magrì A, Di Rosa M, Tomasello M, Guarino F, Reina S, Messina A, et al. Overexpression of human SOD1 in VDAC1-less yeast restores mitochondrial functionality modulating beta-barrel outer membrane protein genes. Biochim Biophys Acta. 2016;1857:789-98 pubmed publisher
    ..Saccharomyces cerevisiae lacking VDAC1 (Δpor1) is a very convenient model system, since it shows an impaired growth rate on non-fermentable carbon source...
  21. Strogolova V, Orlova M, Shevade A, Kuchin S. Mitochondrial porin Por1 and its homolog Por2 contribute to the positive control of Snf1 protein kinase in Saccharomyces cerevisiae. Eukaryot Cell. 2012;11:1568-72 pubmed publisher
    ..We previously recovered the yeast mitochondrial porin Por1 (yVDAC1) from a two-hybrid screen for Snf1-interacting proteins...
  22. Sanada M, Kuroda K, Ueda M. ROS production and apoptosis induction by formation of Gts1p-mediated protein aggregates. Biosci Biotechnol Biochem. 2011;75:1546-53 pubmed
  23. Stroud D, Becker T, Qiu J, Stojanovski D, Pfannschmidt S, Wirth C, et al. Biogenesis of mitochondrial ?-barrel proteins: the POTRA domain is involved in precursor release from the SAM complex. Mol Biol Cell. 2011;22:2823-33 pubmed publisher
    ..These results indicate the POTRA domain of Sam50 is not essential for recognition of ?-barrel precursors but functions in a subsequent step to promote the release of precursor proteins from the SAM complex. ..
  24. Gutiérrez Aguilar M, Pérez Vázquez V, Bunoust O, Manon S, Rigoulet M, Uribe S. In yeast, Ca2+ and octylguanidine interact with porin (VDAC) preventing the mitochondrial permeability transition. Biochim Biophys Acta. 2007;1767:1245-51 pubmed
    ..It is thus suggested that in yeast mitochondria, the voltage-dependent anion channel has a cation binding site where Ca(2+) and octylguanidine interact, conferring cation sensitivity to the yeast mitochondrial unspecific channel. ..