POL3

Summary

Gene Symbol: POL3
Description: DNA-directed DNA polymerase delta POL3
Alias: CDC2, HPR6, TEX1, DNA-directed DNA polymerase delta POL3
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Maloisel L, Fabre F, Gangloff S. DNA polymerase delta is preferentially recruited during homologous recombination to promote heteroduplex DNA extension. Mol Cell Biol. 2008;28:1373-82 pubmed
    ..The pol3-ct allele of the gene encoding the catalytic subunit of DNA polymerase delta (Poldelta) has highlighted a role for ..
  2. Pavlov Y, Shcherbakova P, Kunkel T. In vivo consequences of putative active site mutations in yeast DNA polymerases alpha, epsilon, delta, and zeta. Genetics. 2001;159:47-64 pubmed
    ..The Pol delta mutant (pol3-Y708A) grows slowly, is sensitive to hydroxyurea and methyl methanesulfonate, and is a strong base substitution and ..
  3. Sokolsky T, Alani E. EXO1 and MSH6 are high-copy suppressors of conditional mutations in the MSH2 mismatch repair gene of Saccharomyces cerevisiae. Genetics. 2000;155:589-99 pubmed
    ..In haploids, a msh2Delta mutation is synthetically lethal with pol3-01, a mutation in the Poldelta proofreading exonuclease...
  4. Brocas C, Charbonnier J, Dherin C, Gangloff S, Maloisel L. Stable interactions between DNA polymerase ? catalytic and structural subunits are essential for efficient DNA repair. DNA Repair (Amst). 2010;9:1098-111 pubmed publisher
    ..In Saccharomyces cerevisiae, Pol ? is a heterotrimeric complex composed of the catalytic subunit Pol3, the structural B subunit Pol31, and Pol32, an additional auxiliary subunit...
  5. Herr A, Kennedy S, Knowels G, Schultz E, Preston B. DNA replication error-induced extinction of diploid yeast. Genetics. 2014;196:677-91 pubmed publisher
    ..Double-mutant pol3 alleles that carry mutations for defective DNA polymerase-? proofreading (pol3-01) and accuracy (pol3-L612M or pol3-..
  6. Burgers P, Gerik K. Structure and processivity of two forms of Saccharomyces cerevisiae DNA polymerase delta. J Biol Chem. 1998;273:19756-62 pubmed
    Yeast DNA polymerase delta (Poldelta) consists of three subunits encoded by the POL3, POL31, and POL32 genes...
  7. Acharya N, Klassen R, Johnson R, Prakash L, Prakash S. PCNA binding domains in all three subunits of yeast DNA polymerase ? modulate its function in DNA replication. Proc Natl Acad Sci U S A. 2011;108:17927-32 pubmed publisher
    ..Pol? in Saccharomyces cerevisiae is comprised of three subunits, the catalytic subunit Pol3 and the accessory subunits Pol31 and Pol32...
  8. Hanna J, Kroll E, Lundblad V, Spencer F. Saccharomyces cerevisiae CTF18 and CTF4 are required for sister chromatid cohesion. Mol Cell Biol. 2001;21:3144-58 pubmed
    ..The requirement for CTF4 and CTF18 in robust cohesion identifies novel roles for replication accessory proteins in this process. ..
  9. Iida T, Araki H. Noncompetitive counteractions of DNA polymerase epsilon and ISW2/yCHRAC for epigenetic inheritance of telomere position effect in Saccharomyces cerevisiae. Mol Cell Biol. 2004;24:217-27 pubmed
    ..We therefore propose that inheritance of specific epigenetic states of a telomere requires at least two counteracting regulators. ..

More Information

Publications75

  1. Burgers P. Eukaryotic DNA polymerases in DNA replication and DNA repair. Chromosoma. 1998;107:218-27 pubmed
    ..The role of DNA polymerase beta in base-excision repair is well established for mammalian systems, but in yeast, DNA polymerase delta appears to fulfill that function. ..
  2. Li X, Stith C, Burgers P, Heyer W. PCNA is required for initiation of recombination-associated DNA synthesis by DNA polymerase delta. Mol Cell. 2009;36:704-13 pubmed publisher
    ..We conclude that PCNA has a specific role in the initiation of recombination-associated DNA synthesis and that DNA polymerase delta promotes recombination-associated DNA synthesis. ..
  3. Aksenova A, Volkov K, Maceluch J, Pursell Z, Rogozin I, Kunkel T, et al. Mismatch repair-independent increase in spontaneous mutagenesis in yeast lacking non-essential subunits of DNA polymerase ?. PLoS Genet. 2010;6:e1001209 pubmed publisher
  4. Nick McElhinny S, Gordenin D, Stith C, Burgers P, Kunkel T. Division of labor at the eukaryotic replication fork. Mol Cell. 2008;30:137-44 pubmed publisher
    ..of the leading and lagging strand templates in Saccharomyces cerevisiae using a mutant Pol delta allele (pol3-L612M) whose error rate is higher for one mismatch (e.g., T x dGTP) than for its complement (A x dCTP)...
  5. Swan M, Johnson R, Prakash L, Prakash S, Aggarwal A. Structural basis of high-fidelity DNA synthesis by yeast DNA polymerase delta. Nat Struct Mol Biol. 2009;16:979-86 pubmed publisher
    ..Taken together, the structure provides a chemical basis for the bulk of DNA synthesis in eukaryotic cells and a framework for understanding the effects of cancer-causing mutations in Pol delta. ..
  6. Pavlov Y, Frahm C, Nick McElhinny S, Niimi A, Suzuki M, Kunkel T. Evidence that errors made by DNA polymerase alpha are corrected by DNA polymerase delta. Curr Biol. 2006;16:202-7 pubmed
    ..Given that eukaryotes encode specialized, proofreading-deficient polymerases with even lower fidelity than pol alpha, such intermolecular proofreading could be relevant to several DNA transactions that control genome stability. ..
  7. Tran H, Gordenin D, Resnick M. The prevention of repeat-associated deletions in Saccharomyces cerevisiae by mismatch repair depends on size and origin of deletions. Genetics. 1996;143:1579-87 pubmed
    ..The mutator effects of mutations in the DNA polymerase delta (POL3) gene and the recombinational repair RAD52 gene were studied in combination with mismatch repair defects...
  8. Chanet R, Heude M. Characterization of mutations that are synthetic lethal with pol3-13, a mutated allele of DNA polymerase delta in Saccharomyces cerevisiae. Curr Genet. 2003;43:337-50 pubmed
    The pol3-13 mutation is located in the C-terminal end of POL3, the gene encoding the catalytic subunit of polymerase delta, and confers thermosensitivity onto the Saccharomyces cerevisiae mutant strain...
  9. Morrison A, Sugino A. The 3'-->5' exonucleases of both DNA polymerases delta and epsilon participate in correcting errors of DNA replication in Saccharomyces cerevisiae. Mol Gen Genet. 1994;242:289-96 pubmed
    ..the spontaneous mutator phenotypes of DNA polymerase delta and epsilon 3'-->5' exonuclease-deficient mutants, pol3-01 and pol2-4, respectively...
  10. Jin Y, Obert R, Burgers P, Kunkel T, Resnick M, Gordenin D. The 3'-->5' exonuclease of DNA polymerase delta can substitute for the 5' flap endonuclease Rad27/Fen1 in processing Okazaki fragments and preventing genome instability. Proc Natl Acad Sci U S A. 2001;98:5122-7 pubmed
  11. Johansson E, Majka J, Burgers P. Structure of DNA polymerase delta from Saccharomyces cerevisiae. J Biol Chem. 2001;276:43824-8 pubmed
    DNA polymerase delta (Pol delta) from Saccharomyces cerevisiae consists of three subunits, Pol3 (125 kDa), Pol31 (55 kDa), and Pol32 (40 kDa), present at a 1:1:1 stoichiometry in purified preparations...
  12. Chan C, Galli A, Schiestl R. Pol3 is involved in nonhomologous end-joining in Saccharomyces cerevisiae. DNA Repair (Amst). 2008;7:1531-41 pubmed publisher
    ..The Saccharomyces cerevisiae POL3/CDC2 gene encodes polymerase delta that is involved in DNA replication and other DNA repair processes...
  13. Paek A, Jones H, Kaochar S, Weinert T. The role of replication bypass pathways in dicentric chromosome formation in budding yeast. Genetics. 2010;186:1161-73 pubmed publisher
    ..Fourth, by studying genes implicated in suppression of GCRs in other studies, we found that inverted repeat fusion has a profile of genetic regulation distinct from these other major forms of GCR formation. ..
  14. Jin Y, Ayyagari R, Resnick M, Gordenin D, Burgers P. Okazaki fragment maturation in yeast. II. Cooperation between the polymerase and 3'-5'-exonuclease activities of Pol delta in the creation of a ligatable nick. J Biol Chem. 2003;278:1626-33 pubmed
    ..fragment maturation, exonuclease-deficient polymerase Pol delta-01 and Pol delta-5DV (corresponding to alleles pol3-01-(D321A, E323A) and pol3-5DV-(D520V), respectively) were purified and characterized in this process...
  15. Shah K, Shishkin A, Voineagu I, Pavlov Y, Shcherbakova P, Mirkin S. Role of DNA polymerases in repeat-mediated genome instability. Cell Rep. 2012;2:1088-95 pubmed publisher
    ..The increases in repeat-mediated instability observed in DNA polymerase ? mutants depended on translesion DNA polymerases. We conclude that repeat expansions and RIM are two sides of the same replicative mechanism. ..
  16. Herr A, Ogawa M, Lawrence N, Williams L, Eggington J, Singh M, et al. Mutator suppression and escape from replication error-induced extinction in yeast. PLoS Genet. 2011;7:e1002282 pubmed publisher
    ..Our studies reveal the transient nature of eukaryotic mutators and show that mutator phenotypes are readily suppressed by genetic adaptation. This has implications for the role of mutator phenotypes in cancer. ..
  17. Hishida T, Ohno T, Iwasaki H, Shinagawa H. Saccharomyces cerevisiae MGS1 is essential in strains deficient in the RAD6-dependent DNA damage tolerance pathway. EMBO J. 2002;21:2019-29 pubmed
    ..More over, mgs1 mutation suppresses the temperature sensitivity of mutants in POL3, encoding DNA polymerase delta...
  18. Kokoska R, Stefanovic L, DeMai J, Petes T. Increased rates of genomic deletions generated by mutations in the yeast gene encoding DNA polymerase delta or by decreases in the cellular levels of DNA polymerase delta. Mol Cell Biol. 2000;20:7490-504 pubmed
    In Saccharomyces cerevisiae, POL3 encodes the catalytic subunit of DNA polymerase delta...
  19. Galli A, Cervelli T, Schiestl R. Characterization of the hyperrecombination phenotype of the pol3-t mutation of Saccharomyces cerevisiae. Genetics. 2003;164:65-79 pubmed
    The DNA polymerase delta (Pol3p/Cdc2p) allele pol3-t of Saccharomyces cerevisiae has previously been shown to increase the frequency of deletions between short repeats (several base pairs), between homologous DNA sequences separated by ..
  20. Smith C, Lam A, Symington L. Aberrant double-strand break repair resulting in half crossovers in mutants defective for Rad51 or the DNA polymerase delta complex. Mol Cell Biol. 2009;29:1432-41 pubmed publisher
    ..This outcome is also observed in pol32Delta and pol3-ct mutants, defective for components of the DNA polymerase delta (Pol delta) complex...
  21. Kokoska R, Stefanovic L, Tran H, Resnick M, Gordenin D, Petes T. Destabilization of yeast micro- and minisatellite DNA sequences by mutations affecting a nuclease involved in Okazaki fragment processing (rad27) and DNA polymerase delta (pol3-t). Mol Cell Biol. 1998;18:2779-88 pubmed
    ..in the Saccharomyces cerevisiae RAD27 (encoding a nuclease involved in the processing of Okazaki fragments) and POL3 (encoding DNA polymerase delta) genes on the stability of a minisatellite sequence (20-bp repeats) and ..
  22. Jin Y, Garg P, Stith C, Al Refai H, Sterling J, Murray L, et al. The multiple biological roles of the 3'-->5' exonuclease of Saccharomyces cerevisiae DNA polymerase delta require switching between the polymerase and exonuclease domains. Mol Cell Biol. 2005;25:461-71 pubmed
    ..Mutation rates and spectra and synergistic interactions of the pol3-L523X mutations with msh2, exo1, and rad27/fen1 defects were indistinguishable from those observed with previously ..
  23. Giot L, Chanet R, Simon M, Facca C, Faye G. Involvement of the yeast DNA polymerase delta in DNA repair in vivo. Genetics. 1997;146:1239-51 pubmed
    The POL3 encoded catalytic subunit of DNA polymerase delta possesses a highly conserved C-terminal cysteine-rich domain in Saccharomyces cerevisiae...
  24. Northam M, Garg P, Baitin D, Burgers P, Shcherbakova P. A novel function of DNA polymerase zeta regulated by PCNA. EMBO J. 2006;25:4316-25 pubmed
  25. Gordenin D, Malkova A, Peterzen A, Kulikov V, Pavlov Y, Perkins E, et al. Transposon Tn5 excision in yeast: influence of DNA polymerases alpha, delta, and epsilon and repair genes. Proc Natl Acad Sci U S A. 1992;89:3785-9 pubmed
    ..Mutations of either POL3 or POL1, the proposed structural genes for polymerases delta and alpha, respectively, yield high levels of excision ..
  26. Zhuang Z, Johnson R, Haracska L, Prakash L, Prakash S, Benkovic S. Regulation of polymerase exchange between Poleta and Poldelta by monoubiquitination of PCNA and the movement of DNA polymerase holoenzyme. Proc Natl Acad Sci U S A. 2008;105:5361-6 pubmed publisher
    ..Thus the removal of the ubiquitin moiety from PCNA is likely required for the reverse exchange step after the lesion bypass synthesis by Poleta. ..
  27. Xie Y, Counter C, Alani E. Characterization of the repeat-tract instability and mutator phenotypes conferred by a Tn3 insertion in RFC1, the large subunit of the yeast clamp loader. Genetics. 1999;151:499-509 pubmed
    ..combination with mismatch repair (msh2Delta, pms1Delta), double-strand break repair (rad52), and DNA replication (pol3-01, pol30-52, rth1Delta/rad27Delta) mutations in both forward mutation and repeat-tract instability assays...
  28. Kolodner R, Marsischky G. Eukaryotic DNA mismatch repair. Curr Opin Genet Dev. 1999;9:89-96 pubmed
    ..MMR proteins function in these processes in conjunction with components of nucleotide excision repair (NER) and, possibly, recombination. ..
  29. Pavlov Y, Maki S, Maki H, Kunkel T. Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations. BMC Biol. 2004;2:11 pubmed
    ..This suggests that other polymerases can substitute for certain functions of polymerase epsilon. ..
  30. Simon M, Giot L, Faye G. The 3' to 5' exonuclease activity located in the DNA polymerase delta subunit of Saccharomyces cerevisiae is required for accurate replication. EMBO J. 1991;10:2165-70 pubmed
    In Saccharomyces cerevisiae, DNA polymerase delta (POLIII), the product of the CDC2 (POL3) gene, possesses, in its N-terminal half, the well conserved 3-domain 3' to 5' exonuclease site...
  31. Morrison A, Johnson A, Johnston L, Sugino A. Pathway correcting DNA replication errors in Saccharomyces cerevisiae. EMBO J. 1993;12:1467-73 pubmed
    Mutation of predicted 3'-->5' exonuclease active site residues of Saccharomyces cerevisiae POL3 DNA polymerase (delta) or deletion of the PMS1 mismatch repair gene lead to relative (to wild type) spontaneous mutation rates of ..
  32. Gerik K, Li X, Pautz A, Burgers P. Characterization of the two small subunits of Saccharomyces cerevisiae DNA polymerase delta. J Biol Chem. 1998;273:19747-55 pubmed
    ..The gene for the 125-kDa catalytic subunit (POL3) has been known for several years...
  33. Williams L, Herr A, Preston B. Emergence of DNA polymerase ? antimutators that escape error-induced extinction in yeast. Genetics. 2013;193:751-70 pubmed publisher
    ..to 100-fold increase in spontaneous mutation rate (mutator phenotype), and inactivation of both Pol ? proofreading (pol3-01) and MMR is lethal due to replication error-induced extinction (EEX)...
  34. Li L, Murphy K, Kanevets U, Reha Krantz L. Sensitivity to phosphonoacetic acid: a new phenotype to probe DNA polymerase delta in Saccharomyces cerevisiae. Genetics. 2005;170:569-80 pubmed
    A mutant allele (pol3-L612M) of the DNA polymerase delta gene in Saccharomyces cerevisiae that confers sensitivity to the antiviral drug phosphonoacetic acid (PAA) was constructed...
  35. Drotschmann K, Clark A, Tran H, Resnick M, Gordenin D, Kunkel T. Mutator phenotypes of yeast strains heterozygous for mutations in the MSH2 gene. Proc Natl Acad Sci U S A. 1999;96:2970-5 pubmed
    ..The synergistic effects of heterozygosity for mutations in two different genes that act in series to correct replication errors may be relevant to cancer predisposition. ..
  36. Lis E, O Neill B, Gil Lamaignere C, Chin J, Romesberg F. Identification of pathways controlling DNA damage induced mutation in Saccharomyces cerevisiae. DNA Repair (Amst). 2008;7:801-10 pubmed publisher
  37. Noskov V, Araki H, Sugino A. The RFC2 gene, encoding the third-largest subunit of the replication factor C complex, is required for an S-phase checkpoint in Saccharomyces cerevisiae. Mol Cell Biol. 1998;18:4914-23 pubmed
    ..mutation and that the restrictive temperature of rfc2-1 mutant cells can be lowered by combining either with the cdc2-2 or pol2-11 mutation...
  38. Langston L, O Donnell M. DNA polymerase delta is highly processive with proliferating cell nuclear antigen and undergoes collision release upon completing DNA. J Biol Chem. 2008;283:29522-31 pubmed publisher
    ..The released pol delta transfers to a new primed site, provided the new site contains a PCNA clamp. Additional results indicate that the collision release mechanism is intrinsic to the pol3/pol31 subunits of the pol delta heterotrimer.
  39. Netz D, Stith C, Stümpfig M, Köpf G, Vogel D, Genau H, et al. Eukaryotic DNA polymerases require an iron-sulfur cluster for the formation of active complexes. Nat Chem Biol. 2011;8:125-32 pubmed publisher
    ..Loss of the [4Fe-4S] cofactor by cysteine ligand mutagenesis in Pol3 destabilized the CTD and abrogated interaction with the Pol31 and Pol32 subunits...
  40. Liberti S, LARREA A, Kunkel T. Exonuclease 1 preferentially repairs mismatches generated by DNA polymerase α. DNA Repair (Amst). 2013;12:92-6 pubmed publisher
    ..EXO1 was previously shown to increase mutation rates to a greater extent when combined with a mutator variant (pol3-L612M) of the lagging strand replicase, DNA polymerase δ (Pol δ), than when combined with a mutator variant (..
  41. Formosa T, Nittis T. Suppressors of the temperature sensitivity of DNA polymerase alpha mutations in Saccharomyces cerevisiae. Mol Gen Genet. 1998;257:461-8 pubmed
    ..Both genes, PSP1 and PSP2, also partially suppressed a mutation in POL3 which encodes DNA polymerase delta, and both also affected a mutation in CDC6, which acts in initiation of DNA ..
  42. Kokoska R, Stefanovic L, Buermeyer A, Liskay R, Petes T. A mutation of the yeast gene encoding PCNA destabilizes both microsatellite and minisatellite DNA sequences. Genetics. 1999;151:511-9 pubmed
    ..of repetitive DNA with large repeat unit lengths was similar, but not identical, to that observed previously for pol3-t, a temperature-sensitive mutation affecting DNA polymerase delta...
  43. Storici F, Bebenek K, Kunkel T, Gordenin D, Resnick M. RNA-templated DNA repair. Nature. 2007;447:338-41 pubmed
  44. Daraba A, Gali V, Halmai M, Haracska L, Unk I. Def1 promotes the degradation of Pol3 for polymerase exchange to occur during DNA-damage--induced mutagenesis in Saccharomyces cerevisiae. PLoS Biol. 2014;12:e1001771 pubmed publisher
    ..we demonstrate that upon DNA damage, Def1 promotes the ubiquitylation and subsequent proteasomal degradation of Pol3, the catalytic subunit of the replicative polymerase ?, whereas Pol31 and Pol32, the other two subunits of ..
  45. Branzei D, Seki M, Enomoto T. Rad18/Rad5/Mms2-mediated polyubiquitination of PCNA is implicated in replication completion during replication stress. Genes Cells. 2004;9:1031-42 pubmed
    ..that the rad18delta mutation suppresses the temperature sensitivity of the polymerase delta mutants hys2-1 and cdc2-1 as well as the synthetic lethality of cdc2-1 pol32delta mutants, suggesting a role for Rad18 in modulating DNA ..
  46. Conover H, Lujan S, Chapman M, Cornelio D, Sharif R, Williams J, et al. Stimulation of Chromosomal Rearrangements by Ribonucleotides. Genetics. 2015;201:951-61 pubmed publisher
    ..The LOH rate was not elevated in strains carrying the pol1-L868M or pol3-L612M alleles that result in increased incorporation of ribonucleotides during DNA synthesis by polymerases α ..
  47. Meyer D, Fu B, Heyer W. DNA polymerases δ and λ cooperate in repairing double-strand breaks by microhomology-mediated end-joining in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2015;112:E6907-16 pubmed publisher
    ..Moreover, Pol δ recruitment was diminished in cells lacking Pol λ. These data suggest cooperative involvement of both polymerases in MMEJ. ..
  48. Gary R, Park M, Nolan J, Cornelius H, Kozyreva O, Tran H, et al. A novel role in DNA metabolism for the binding of Fen1/Rad27 to PCNA and implications for genetic risk. Mol Cell Biol. 1999;19:5373-82 pubmed
    ..Furthermore, the PCNA binding mutation resulted in lethality when combined with a homozygous or even a heterozygous pol3-01 mutation in the 3'-->5' exonuclease domain of DNA polymerase delta...
  49. Rossi M, Bambara R. Reconstituted Okazaki fragment processing indicates two pathways of primer removal. J Biol Chem. 2006;281:26051-61 pubmed
    ..However, some flaps become long and require the two-nuclease pathway. It appears that both pathways, operating in parallel, are required for processing of all flaps. ..
  50. Saini N, Zhang Y, Nishida Y, Sheng Z, Choudhury S, Mieczkowski P, et al. Fragile DNA motifs trigger mutagenesis at distant chromosomal loci in saccharomyces cerevisiae. PLoS Genet. 2013;9:e1003551 pubmed publisher
    ..These data demonstrate that repeat-mediated breaks pose a dual threat to eukaryotic genome integrity by inducing chromosomal aberrations as well as mutations in flanking genes...
  51. Johnson R, Klassen R, Prakash L, Prakash S. A Major Role of DNA Polymerase δ in Replication of Both the Leading and Lagging DNA Strands. Mol Cell. 2015;59:163-175 pubmed publisher
    Genetic studies with S. cerevisiae Polδ (pol3-L612M) and Polε (pol2-M644G) mutant alleles, each of which display a higher rate for the generation of a specific mismatch, have led to the conclusion that Polε is the primary ..
  52. Hombauer H, Campbell C, Smith C, Desai A, Kolodner R. Visualization of eukaryotic DNA mismatch repair reveals distinct recognition and repair intermediates. Cell. 2011;147:1040-53 pubmed publisher
    ..These findings suggest the presence of replication machinery-coupled and -independent pathways for mispair recognition by Msh2-Msh6, which direct formation of superstoichiometric Mlh1-Pms1 foci that represent sites of active MMR. ..
  53. Kim H, Livingston D. Suppression of a DNA polymerase delta mutation by the absence of the high mobility group protein Hmo1 in Saccharomyces cerevisiae. Curr Genet. 2009;55:127-38 pubmed publisher
    ..gene encoding the high mobility group protein Hmo1 suppresses the growth retardation of the DNA pol delta mutation, pol3-14, at the restrictive temperature...
  54. Hryciw T, Tang M, Fontanie T, Xiao W. MMS1 protects against replication-dependent DNA damage in Saccharomyces cerevisiae. Mol Genet Genomics. 2002;266:848-57 pubmed
    ..that induce replication-dependent double-strand breaks, such as treatment with camptothecin, and incubation of a cdc2-2 strain at the restrictive temperature...
  55. Burkovics P, Sebesta M, Sisakova A, Plault N, Szukacsov V, Robert T, et al. Srs2 mediates PCNA-SUMO-dependent inhibition of DNA repair synthesis. EMBO J. 2013;32:742-55 pubmed publisher
    ..Our results suggest that cycling cells use the Siz1-dependent SUMOylation of PCNA to limit the extension of repair synthesis during template switch or HR and attenuate reciprocal DNA strand exchanges to maintain genome stability. ..
  56. Biswas D, Takahata S, Xin H, Dutta Biswas R, Yu Y, Formosa T, et al. A role for Chd1 and Set2 in negatively regulating DNA replication in Saccharomyces cerevisiae. Genetics. 2008;178:649-59 pubmed publisher
    ..chd1 mutations also suppress the HU sensitivity of mutations in other genes involved in DNA replication, including CDC2, CTF4, ORC2, and MEC1...
  57. Carroll C, Enquist Newman M, Morgan D. The APC subunit Doc1 promotes recognition of the substrate destruction box. Curr Biol. 2005;15:11-8 pubmed
    ..Doc1 contributes to recognition of the substrate destruction box by the APC. This function of Doc1 is necessary for efficient substrate proteolysis in vivo. ..
  58. Zhang L, Huang Y, Zhu X, Wang Y, Shi H, Chen M, et al. Role of Translesion Synthesis DNA Polymerases in DNA Replication in the Presence of a Weak DNA Polymerase ? in Saccharomyces cerevisiae. G3 (Bethesda). 2018;: pubmed publisher
    ..Zhang's requested removal of the authors.Both Dr. Zhang and G3 apologize to our readers for any inconvenience caused by this retraction. ..
  59. Mito E, Mokhnatkin J, Steele M, Buettner V, Sommer S, Manthey G, et al. Mutagenic and recombinagenic responses to defective DNA polymerase delta are facilitated by the Rev1 protein in pol3-t mutants of Saccharomyces cerevisiae. Genetics. 2008;179:1795-806 pubmed publisher
    ..In the yeast Saccharomyces cerevisiae, the pol3-t allele confers a defect in the catalytic subunit of replicative DNA polymerase delta that results in increased ..
  60. Williams J, Clausen A, Lujan S, Marjavaara L, Clark A, Burgers P, et al. Evidence that processing of ribonucleotides in DNA by topoisomerase 1 is leading-strand specific. Nat Struct Mol Biol. 2015;22:291-7 pubmed publisher
  61. Argueso J, Smith D, Yi J, Waase M, Sarin S, Alani E. Analysis of conditional mutations in the Saccharomyces cerevisiae MLH1 gene in mismatch repair and in meiotic crossing over. Genetics. 2002;160:909-21 pubmed
    ..In this report we show that in Saccharomyces cerevisiae the mlh1Delta mutation confers inviability in pol3-01 strain backgrounds that are defective in the Poldelta proofreading exonuclease activity...
  62. Kochenova O, Bezalel Buch R, Tran P, Makarova A, Chabes A, Burgers P, et al. Yeast DNA polymerase ? maintains consistent activity and mutagenicity across a wide range of physiological dNTP concentrations. Nucleic Acids Res. 2017;45:1200-1218 pubmed publisher
    ..The results support a model wherein dNTP elevation is needed to facilitate non-mutagenic tolerance pathways, while Pol? synthesis represents a unique mechanism of rescuing stalled replication when dNTP supply is low. ..
  63. Kadyrova L, Mertz T, Zhang Y, Northam M, Sheng Z, Lobachev K, et al. A reversible histone H3 acetylation cooperates with mismatch repair and replicative polymerases in maintaining genome stability. PLoS Genet. 2013;9:e1003899 pubmed publisher
    ..Our results suggest that cyclic acetylation and deacetylation of chromatin contribute to replication fidelity and play important roles in the protection of nuclear DNA from diverse spontaneous mutations. ..
  64. Darmawan H, Harrison M, Reha Krantz L. DNA polymerase 3'→5' exonuclease activity: Different roles of the beta hairpin structure in family-B DNA polymerases. DNA Repair (Amst). 2015;29:36-46 pubmed publisher
    ..Thus, while family B DNA polymerases share conserved motifs and general structural features, the β hairpin has evolved to meet specific needs. ..
  65. Hashimoto K, Nakashima N, Ohara T, Maki S, Sugino A. The second subunit of DNA polymerase III (delta) is encoded by the HYS2 gene in Saccharomyces cerevisiae. Nucleic Acids Res. 1998;26:477-85 pubmed
    ..and find that they match parts of the amino acid sequences predicted from the nucleotide sequence of the CDC2 and HYS2 genes respectively...
  66. Stepchenkova E, Tarakhovskaya E, Siebler H, Pavlov Y. Defect of Fe-S cluster binding by DNA polymerase ? in yeast suppresses UV-induced mutagenesis, but enhances DNA polymerase ? - dependent spontaneous mutagenesis. DNA Repair (Amst). 2017;49:60-69 pubmed publisher
    ..Intriguingly, the pol3-13 mutation that affects the Fe-S cluster in the CTD of the catalytic subunit of pol ? also leads to defective ..