Gene Symbol: PMS1
Description: ATP-binding mismatch repair protein
Alias: ATP-binding mismatch repair protein
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Chambers S, Hunter N, Louis E, Borts R. The mismatch repair system reduces meiotic homeologous recombination and stimulates recombination-dependent chromosome loss. Mol Cell Biol. 1996;16:6110-20 pubmed
    ..This phenomenon is dependent on the activities of the mismatch repair genes PMS1 and MSH2. A model of mismatch-stimulated chromosome loss is proposed to account for this observation...
  2. Pang Q, Prolla T, Liskay R. Functional domains of the Saccharomyces cerevisiae Mlh1p and Pms1p DNA mismatch repair proteins and their relevance to human hereditary nonpolyposis colorectal cancer-associated mutations. Mol Cell Biol. 1997;17:4465-73 pubmed
    ..In addition, certain MLH1 and PMS1 mutant alleles caused a dominant negative mutator effect when overexpressed...
  3. Umar A, Buermeyer A, Simon J, Thomas D, Clark A, Liskay R, et al. Requirement for PCNA in DNA mismatch repair at a step preceding DNA resynthesis. Cell. 1996;87:65-73 pubmed
    ..The ability of PCNA to bind to MLH1 and MSH2 may reflect linkage between mismatch repair and replication and may be relevant to the roles of mismatch repair proteins in other DNA transactions. ..
  4. Prolla T, Pang Q, Alani E, Kolodner R, Liskay R. MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast. Science. 1994;265:1091-3 pubmed
    ..In the yeast Saccharomyces cerevisiae, DNA mismatch repair requires the MSH2, MLH1, and PMS1 proteins...
  5. Amin N, Nguyen M, Oh S, Kolodner R. exo1-Dependent mutator mutations: model system for studying functional interactions in mismatch repair. Mol Cell Biol. 2001;21:5142-55 pubmed identify mutations that increase the mutation rates caused by weak mutator mutations such as exo1Delta and pms1-A130V mutations...
  6. Wang T, Kleckner N, Hunter N. Functional specificity of MutL homologs in yeast: evidence for three Mlh1-based heterocomplexes with distinct roles during meiosis in recombination and mismatch correction. Proc Natl Acad Sci U S A. 1999;96:13914-9 pubmed
    The yeast genome encodes four proteins (Pms1 and Mlh1-3) homologous to the bacterial mismatch repair component, MutL...
  7. Mendillo M, Mazur D, Kolodner R. Analysis of the interaction between the Saccharomyces cerevisiae MSH2-MSH6 and MLH1-PMS1 complexes with DNA using a reversible DNA end-blocking system. J Biol Chem. 2005;280:22245-57 pubmed
    ..The MLH1-PMS1 complex formed both mispair-dependent and mispair-independent ternary complexes with the MSH2-MSH6 complex on DNA...
  8. Srivatsan A, Bowen N, Kolodner R. Mispair-specific recruitment of the Mlh1-Pms1 complex identifies repair substrates of the Saccharomyces cerevisiae Msh2-Msh3 complex. J Biol Chem. 2014;289:9352-64 pubmed publisher
    ..and performed a comparative study of Msh2-Msh3 and Msh2-Msh6 for mispair binding, sliding clamp formation, and Mlh1-Pms1 recruitment...
  9. Gueneau E, Dherin C, Legrand P, Tellier Lebegue C, Gilquin B, Bonnesoeur P, et al. Structure of the MutL? C-terminal domain reveals how Mlh1 contributes to Pms1 endonuclease site. Nat Struct Mol Biol. 2013;20:461-8 pubmed publisher
    ..Here we report crystal structures of the MutL? (Mlh1-Pms1 heterodimer) C-terminal domain (CTD) from Saccharomyces cerevisiae, alone and in complex with fragments derived ..

More Information


  1. Romanova N, Crouse G. Different roles of eukaryotic MutS and MutL complexes in repair of small insertion and deletion loops in yeast. PLoS Genet. 2013;9:e1003920 pubmed publisher
    ..Two mutants of MutL?, pms1-G882E and pms1-H888R, repair deletion mispairs but not insertion mispairs...
  2. Erdeniz N, Dudley S, Gealy R, Jinks Robertson S, Liskay R. Novel PMS1 alleles preferentially affect the repair of primer strand loops during DNA replication. Mol Cell Biol. 2005;25:9221-31 pubmed
    ..We identified two novel yeast pms1 alleles, pms1-G882E and pms1-H888R, which confer a strong defect in the repair of "primer strand" loops, ..
  3. Xie Y, Counter C, Alani E. Characterization of the repeat-tract instability and mutator phenotypes conferred by a Tn3 insertion in RFC1, the large subunit of the yeast clamp loader. Genetics. 1999;151:499-509 pubmed
    ..From these data we propose that defects in clamp loader function result in DNA replication errors, a subset of which are acted upon by the mismatch-repair system. ..
  4. Li L, Murphy K, Kanevets U, Reha Krantz L. Sensitivity to phosphonoacetic acid: a new phenotype to probe DNA polymerase delta in Saccharomyces cerevisiae. Genetics. 2005;170:569-80 pubmed
    ..Reduced viability is observed in the absence of any of the core MMR proteins-Msh2, Mlh1, or Pms1-and severe sensitivity to PAA is observed in the absence of the core proteins Msh6 or Exo1, but not Msh3...
  5. Morrison A, Johnson A, Johnston L, Sugino A. Pathway correcting DNA replication errors in Saccharomyces cerevisiae. EMBO J. 1993;12:1467-73 pubmed
    ..exonuclease active site residues of Saccharomyces cerevisiae POL3 DNA polymerase (delta) or deletion of the PMS1 mismatch repair gene lead to relative (to wild type) spontaneous mutation rates of approximately 130 and 41, ..
  6. Stone J, Petes T. Analysis of the proteins involved in the in vivo repair of base-base mismatches and four-base loops formed during meiotic recombination in the yeast Saccharomyces cerevisiae. Genetics. 2006;173:1223-39 pubmed
    ..loop; repair of the four-base loop was affected by loss of the Mlh3p, and the repair defect of the mlh1 and pms1 strains was significantly smaller than that observed in the msh2 strain...
  7. Alekseev S, Kovaltsova S, Fedorova I, Gracheva L, Evstukhina T, Peshekhonov V, et al. HSM2 (HMO1) gene participates in mutagenesis control in yeast Saccharomyces cerevisiae. DNA Repair (Amst). 2002;1:287-97 pubmed
    ..and a mutation in a gene of principal damaged DNA repair pathways (rad2 and rev3) or in a mismatch repair gene (pms1 and recently characterized in our laboratory hsm3)...
  8. Gendrel C, Dutreix M. (CA/TG) microsatellite sequences escape the inhibition of recombination by mismatch repair in Saccharomyces cerevisiae. Genetics. 2001;159:1539-45 pubmed
    ..demonstrate that: (1) the inhibition of conversion by large inserts depends upon a complex containing both Msh2 and Pms1 proteins; (2) conversion is not inhibited if the single-stranded DNA loop in the heteroduplex is the microsatellite ..
  9. Zhang L, Huang Y, Zhu X, Wang Y, Shi H, Chen M, et al. Role of Translesion Synthesis DNA Polymerases in DNA Replication in the Presence of a Weak DNA Polymerase ? in Saccharomyces cerevisiae. G3 (Bethesda). 2018;: pubmed publisher
    ..Zhang's requested removal of the authors.Both Dr. Zhang and G3 apologize to our readers for any inconvenience caused by this retraction. ..
  10. Hall M, Kunkel T. Purification of eukaryotic MutL homologs from Saccharomyces cerevisiae using self-affinity technology. Protein Expr Purif. 2001;21:333-42 pubmed
    ..Mlh1 and Pms1 are homologs of the E...
  11. Tran H, Gordenin D, Resnick M. The prevention of repeat-associated deletions in Saccharomyces cerevisiae by mismatch repair depends on size and origin of deletions. Genetics. 1996;143:1579-87 pubmed
    ..In a rad52 null mutant only the 1-bp deletions were increased (12-fold). The mismatch repair mutations pms1, msh2 and msh3 did not affect 31- and 61-bp deletions in the pol3-t but increased the rates of 7- and 1-bp ..
  12. Habraken Y, Sung P, Prakash L, Prakash S. ATP-dependent assembly of a ternary complex consisting of a DNA mismatch and the yeast MSH2-MSH6 and MLH1-PMS1 protein complexes. J Biol Chem. 1998;273:9837-41 pubmed
    MSH2 and MSH6 proteins exist as a stable complex, as do the MLH1 and PMS1 proteins...
  13. Eissenberg J, Ayyagari R, Gomes X, Burgers P. Mutations in yeast proliferating cell nuclear antigen define distinct sites for interaction with DNA polymerase delta and DNA polymerase epsilon. Mol Cell Biol. 1997;17:6367-78 pubmed
  14. Habraken Y, Sung P, Prakash L, Prakash S. Enhancement of MSH2-MSH3-mediated mismatch recognition by the yeast MLH1-PMS1 complex. Curr Biol. 1997;7:790-3 pubmed
    ..These complexes have, to some extent, overlapping mismatch binding specificities. MLH1 and PMS1 are the other essential components of mismatch repair, but how they function in this process is not known...
  15. Tran P, Liskay R. Functional studies on the candidate ATPase domains of Saccharomyces cerevisiae MutLalpha. Mol Cell Biol. 2000;20:6390-8 pubmed
    ..Taken together, these results suggest that MutLalpha undergoes ATP-dependent conformational changes that may serve to coordinate downstream events during yeast DNA mismatch repair. ..
  16. Merchan S, Pedelini L, Hueso G, Calzada A, Serrano R, Yenush L. Genetic alterations leading to increases in internal potassium concentrations are detrimental for DNA integrity in Saccharomyces cerevisiae. Genes Cells. 2011;16:152-65 pubmed publisher
  17. Vedel M, Nicolas A. CYS3, a hotspot of meiotic recombination in Saccharomyces cerevisiae. Effects of heterozygosity and mismatch repair functions on gene conversion and recombination intermediates. Genetics. 1999;151:1245-59 pubmed
    ..This gradient is relieved in the presence of msh2 and pms1 mutations, indicating an involvement of mismatch repair functions in meiotic recombination...
  18. Chernenkov A, Gracheva L, Evstiukhina T, Koval tsova S, Peshekhonov V, Fedorova I, et al. [Interaction of gene HSM3 with genes of the epistatic RAD6 group in yeast Saccharomyces cerevisiae]. Genetika. 2012;48:160-7 pubmed
    ..Our results demonstrated that the Hsm3 protein contains at least two domains; the N-terminal part of the domain is responsible for the proteasome assembly, whereas the C-terminal portion of the protein is responsible for mutagenesis. ..
  19. Hall M, Shcherbakova P, Kunkel T. Differential ATP binding and intrinsic ATP hydrolysis by amino-terminal domains of the yeast Mlh1 and Pms1 proteins. J Biol Chem. 2002;277:3673-9 pubmed
    ..We demonstrate that amino-terminal domains of the yeast MutL homologs Mlh1 and Pms1 required for DNA mismatch repair both possess independent, intrinsic ATPase activities...
  20. Shcherbakova P, Hall M, Lewis M, Bennett S, Martin K, Bushel P, et al. Inactivation of DNA mismatch repair by increased expression of yeast MLH1. Mol Cell Biol. 2001;21:940-51 pubmed
    ..DNA polymerases delta and epsilon but did not enhance the mutation rate in strains with deletions of MSH2, MLH1, or PMS1. This suggests that overexpression of MLH1 inactivates mismatch repair of replication errors...
  21. Welz Voegele C, Stone J, Tran P, Kearney H, Liskay R, Petes T, et al. Alleles of the yeast Pms1 mismatch-repair gene that differentially affect recombination- and replication-related processes. Genetics. 2002;162:1131-45 pubmed
    ..the processing steps in the spellchecker and antirecombination pathways might differ, we mutagenized the yeast PMS1 gene and screened for mitotic separation-of-function alleles...
  22. Fedorova I, Kovaltzova S, Gracheva L, Evstuhina T, Korolev V. Requirement of HSM3 gene for spontaneous mutagenesis in Saccharomyces cerevisiae. Mutat Res. 2004;554:67-75 pubmed
    ..of accumulation of spontaneous mutants was observed in slowly dividing cells in the rad1, rad2, rad14, rad54, and pms1, but it was absent in the rev3, pol2 and pol3 mutants...
  23. Kelberg E, Kovaltsova S, Alekseev S, Fedorova I, Gracheva L, Evstukhina T, et al. HIM1, a new yeast Saccharomyces cerevisiae gene playing a role in control of spontaneous and induced mutagenesis. Mutat Res. 2005;578:64-78 pubmed
    ..genes: apn1, blocking base excision repair; rad2, rev3, and rad54, blocking three principal DNA repair pathways; pms1, blocking mismatch repair; hsm2 and hsm3 mutations, which lead to a mutator effect...
  24. Arana M, Holmes S, Fortune J, Moon A, Pedersen L, Kunkel T. Functional residues on the surface of the N-terminal domain of yeast Pms1. DNA Repair (Amst). 2010;9:448-57 pubmed publisher
    Saccharomyces cerevisiae MutLalpha is a heterodimer of Mlh1 and Pms1 that participates in DNA mismatch repair (MMR)...
  25. Gammie A, Erdeniz N, Beaver J, Devlin B, Nanji A, Rose M. Functional characterization of pathogenic human MSH2 missense mutations in Saccharomyces cerevisiae. Genetics. 2007;177:707-21 pubmed
    ..This analysis underscores the importance of functional characterization of missense alleles to ensure that they are the causative factor for disease. ..
  26. Kramer B, Kramer W, Williamson M, Fogel S. Heteroduplex DNA correction in Saccharomyces cerevisiae is mismatch specific and requires functional PMS genes. Mol Cell Biol. 1989;9:4432-40 pubmed
    ..Repair of the listed mismatches was severely impaired in the putative S. cerevisiae DNA mismatch repair mutants pms1 and pms2...
  27. Tran P, Simon J, Liskay R. Interactions of Exo1p with components of MutLalpha in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2001;98:9760-5 pubmed
    ..Finally, our results show that much of the increase in spontaneous mutation observed in an exo1Delta strain is REV3-dependent, in turn suggesting that Exo1p is also involved in one or more MMR-independent mutation avoidance pathways. ..
  28. Nicholson A, Hendrix M, Jinks Robertson S, Crouse G. Regulation of mitotic homeologous recombination in yeast. Functions of mismatch repair and nucleotide excision repair genes. Genetics. 2000;154:133-46 pubmed
    ..In addition to the yeast MutS and MutL homologs, the exonuclease Exo1p and the nucleotide excision repair proteins Rad1p and Rad10p were found to have roles in inhibiting recombination between mismatched substrates. ..
  29. Argueso J, Smith D, Yi J, Waase M, Sarin S, Alani E. Analysis of conditional mutations in the Saccharomyces cerevisiae MLH1 gene in mismatch repair and in meiotic crossing over. Genetics. 2002;160:909-21 pubmed
    ..These genetic studies suggest that the conditional mlh1 mutations can be used to separate the MMR and meiotic crossing-over functions of MLH1 and to identify interactions between MLH1 and downstream repair components. ..
  30. Sacho E, Kadyrov F, Modrich P, Kunkel T, Erie D. Direct visualization of asymmetric adenine-nucleotide-induced conformational changes in MutL alpha. Mol Cell. 2008;29:112-21 pubmed publisher
    ..These data reveal an ATPase cycle in which sequential nucleotide binding, hydrolysis, and release modulate the conformational states of MutL alpha. ..
  31. Heck J, Argueso J, Gemici Z, Reeves R, Bernard A, Aquadro C, et al. Negative epistasis between natural variants of the Saccharomyces cerevisiae MLH1 and PMS1 genes results in a defect in mismatch repair. Proc Natl Acad Sci U S A. 2006;103:3256-61 pubmed
    In budding yeast, the MLH1-PMS1 heterodimer is the major MutL homolog complex that acts to repair mismatches arising during DNA replication...
  32. Hall M, Wang H, Erie D, Kunkel T. High affinity cooperative DNA binding by the yeast Mlh1-Pms1 heterodimer. J Mol Biol. 2001;312:637-47 pubmed
    We demonstrate here that the Saccharomyces cerevisiae Mlh1-Pms1 heterodimer required for DNA mismatch repair and other cellular processes is a DNA binding protein...
  33. Tran P, Fey J, Erdeniz N, Gellon L, Boiteux S, Liskay R. A mutation in EXO1 defines separable roles in DNA mismatch repair and post-replication repair. DNA Repair (Amst). 2007;6:1572-83 pubmed
    ..Lastly, by using a compound exo1 mutant that was defective for interaction with Mlh1p and deficient for nuclease activity, we provide further evidence that Exo1p plays both structural and catalytic roles during MMR. ..
  34. Williams L, Herr A, Preston B. Emergence of DNA polymerase ? antimutators that escape error-induced extinction in yeast. Genetics. 2013;193:751-70 pubmed publisher
    ..we observed synthetic lethality of pol2-4 with alleles that completely abrogate MMR (msh2?, mlh1?, msh3? msh6?, or pms1? mlh3?) but not with partial MMR loss (msh3?, msh6?, pms1?, or mlh3?), indicating that high levels of unrepaired ..
  35. Kadyrova L, Dahal B, Kadyrov F. Evidence that the DNA mismatch repair system removes 1-nucleotide Okazaki fragment flaps. J Biol Chem. 2015;290:24051-65 pubmed publisher
    ..ATPase and endonuclease mutants of MutLα are defective in the flap removal. These results suggest that the MMR system contributes to the removal of 1-nucleotide Okazaki fragment flaps. ..
  36. Plys A, Rogacheva M, Greene E, Alani E. The unstructured linker arms of Mlh1-Pms1 are important for interactions with DNA during mismatch repair. J Mol Biol. 2012;422:192-203 pubmed publisher
    ..MLH (MutL homolog) proteins (primarily Mlh1-Pms1 in baker's yeast) then survey the genome for lesion-bound MSH proteins...
  37. Martinez S, Kolodner R. Functional analysis of human mismatch repair gene mutations identifies weak alleles and polymorphisms capable of polygenic interactions. Proc Natl Acad Sci U S A. 2010;107:5070-5 pubmed publisher
    ..These results indicate that weak MMR gene alleles capable of polygenic interactions with other MMR gene alleles may be relatively common. ..
  38. Smith C, Mendillo M, Bowen N, Hombauer H, Campbell C, Desai A, et al. Dominant mutations in S. cerevisiae PMS1 identify the Mlh1-Pms1 endonuclease active site and an exonuclease 1-independent mismatch repair pathway. PLoS Genet. 2013;9:e1003869 pubmed publisher
    ..To better understand the function of Mlh1-Pms1 in MMR, we used Saccharomyces cerevisiae to identify six pms1 mutations (pms1-G683E, pms1-C817R, pms1-C848S, pms1-..
  39. Saparbaev M, Prakash L, Prakash S. Requirement of mismatch repair genes MSH2 and MSH3 in the RAD1-RAD10 pathway of mitotic recombination in Saccharomyces cerevisiae. Genetics. 1996;142:727-36 pubmed
    ..Coupling of mismatch binding proteins with the recombinational machinery could be important for ensuring genetic fidelity in the recombination process. ..
  40. Lehner K, Jinks Robertson S. The mismatch repair system promotes DNA polymerase zeta-dependent translesion synthesis in yeast. Proc Natl Acad Sci U S A. 2009;106:5749-54 pubmed publisher
    ..Finally, in contrast to its ability to remove mistakes made by replicative DNA polymerases, we show that MMR fails to efficiently correct errors introduced by Polzeta. ..
  41. Campbell C, Hombauer H, Srivatsan A, Bowen N, Gries K, Desai A, et al. Mlh2 is an accessory factor for DNA mismatch repair in Saccharomyces cerevisiae. PLoS Genet. 2014;10:e1004327 pubmed publisher
    In Saccharomyces cerevisiae, the essential mismatch repair (MMR) endonuclease Mlh1-Pms1 forms foci promoted by Msh2-Msh6 or Msh2-Msh3 in response to mispaired bases...
  42. Goellner E, Smith C, Campbell C, Hombauer H, Desai A, Putnam C, et al. PCNA and Msh2-Msh6 activate an Mlh1-Pms1 endonuclease pathway required for Exo1-independent mismatch repair. Mol Cell. 2014;55:291-304 pubmed publisher
    ..has implicated multiple pathways in eukaryotic DNA mismatch repair (MMR) downstream of mispair recognition and Mlh1-Pms1 recruitment, including Exonuclease 1 (Exo1)-dependent and -independent pathways...
  43. Dherin C, Gueneau E, Francin M, Nunez M, Miron S, Liberti S, et al. Characterization of a highly conserved binding site of Mlh1 required for exonuclease I-dependent mismatch repair. Mol Cell Biol. 2009;29:907-18 pubmed publisher
    ..critical for the interaction with Exo1, Ntg2, and Sgs1 proteins, designated as site S2 by reference to the Mlh1/Pms1 heterodimerization site S1...
  44. Duroc Y, Kumar R, Ranjha L, Adam C, Guérois R, Md Muntaz K, et al. Concerted action of the MutLβ heterodimer and Mer3 helicase regulates the global extent of meiotic gene conversion. elife. 2017;6: pubmed publisher
    ..These findings show that MutLβ is an integral part of a new regulatory step of meiotic recombination, which has implications to prevent rapid allele fixation and hotspot erosion in populations. ..
  45. Pavlov Y, Nguyen D, Kunkel T. Mutator effects of overproducing DNA polymerase eta (Rad30) and its catalytically inactive variant in yeast. Mutat Res. 2001;478:129-39 pubmed
    ..Moreover, while excess wild-type Pol eta primarily induced base substitutions in the msh6 and pms1 strains, excess inactive Rad30p induced both base substitutions and frameshifts...