Gene Symbol: PMA1
Description: H(+)-exporting P2-type ATPase PMA1
Alias: KTI10, H(+)-exporting P2-type ATPase PMA1
Species: Saccharomyces cerevisiae S288c
Products:     PMA1

Top Publications

  1. Carmelo V, Bogaerts P, Sá Correia I. Activity of plasma membrane H+-ATPase and expression of PMA1 and PMA2 genes in Saccharomyces cerevisiae cells grown at optimal and low pH. Arch Microbiol. 1996;166:315-20 pubmed
    ..The level of mRNA from the essential plasma-membrane-ATPase-encoding gene PMA1 was reduced by 50% in cells grown at pH 2.5 as compared with cells grown at the optimal pH 5...
  2. Bagnat M, Chang A, Simons K. Plasma membrane proton ATPase Pma1p requires raft association for surface delivery in yeast. Mol Biol Cell. 2001;12:4129-38 pubmed
    ..Disruption of rafts led to mistargeting of Pma1p to the vacuole. Conversely, Pma1-7, an ATPase mutant that is mistargeted to the vacuole, was shown to exhibit impaired raft association...
  3. Eraso P, Mazón M, Posas F, Portillo F. Gene expression profiling of yeasts overexpressing wild type or misfolded Pma1 variants reveals activation of the Hog1 MAPK pathway. Mol Microbiol. 2011;79:1339-52 pubmed publisher
    Dominant negative PMA1 mutants render misfolded proteins that are retained in the endoplasmic reticulum (ER) and slowly degraded by ER-associated degradation...
  4. Wang Q, Chang A. Sphingoid base synthesis is required for oligomerization and cell surface stability of the yeast plasma membrane ATPase, Pma1. Proc Natl Acad Sci U S A. 2002;99:12853-8 pubmed
    The plasma membrane H(+)-ATPase, Pma1, is an essential and long-lived integral membrane protein...
  5. Chang A, Fink G. Targeting of the yeast plasma membrane [H+]ATPase: a novel gene AST1 prevents mislocalization of mutant ATPase to the vacuole. J Cell Biol. 1995;128:39-49 pubmed
    We have characterized a class of mutations in PMA1, (encoding plasma membrane ATPase) that is ideal for the analysis of membrane targeting in Saccharomyces cerevisiae...
  6. Young B, Shin J, Orij R, Chao J, Li S, Guan X, et al. Phosphatidic acid is a pH biosensor that links membrane biogenesis to metabolism. Science. 2010;329:1085-8 pubmed publisher
    ..This enabled coupling of membrane biogenesis to nutrient availability. ..
  7. Wang Q, Chang A. Eps1, a novel PDI-related protein involved in ER quality control in yeast. EMBO J. 1999;18:5972-82 pubmed
    b>PMA1 is an essential gene encoding the yeast plasma membrane [H(+)]ATPase...
  8. Bagnat M, Keranen S, Shevchenko A, Simons K. Lipid rafts function in biosynthetic delivery of proteins to the cell surface in yeast. Proc Natl Acad Sci U S A. 2000;97:3254-9 pubmed
    ..Using the sec18-1 mutant we found that DIGs are present already in the ER. Taken together, our results suggest that lipid rafts are involved in the biosynthetic delivery of proteins to the yeast plasma membrane. ..
  9. Eraso P, Mazón M, Portillo F. A dominant negative mutant of PMA1 interferes with the folding of the wild type enzyme. Traffic. 2010;11:37-47 pubmed publisher
    ..Several mutant alleles of PMA1, the gene coding for the plasma membrane H (+)-ATPase, render misfolded proteins that are subjected to ERAD...

More Information


  1. Chan G, Hardej D, Santoro M, Lau Cam C, Billack B. Evaluation of the antimicrobial activity of ebselen: role of the yeast plasma membrane H+-ATPase. J Biochem Mol Toxicol. 2007;21:252-64 pubmed
    ..In addition, ebselen was found to inhibit medium acidification by PMA1-proficient haploid yeast in a concentration-dependent manner...
  2. Huang C, Chang A. pH-dependent cargo sorting from the Golgi. J Biol Chem. 2011;286:10058-65 pubmed publisher subunit deletion or glucose deprivation results in missorting of newly synthesized plasma membrane proteins Pma1 and Can1 directly from the Golgi to the vacuole...
  3. Withee J, Sen R, Cyert M. Ion tolerance of Saccharomyces cerevisiae lacking the Ca2+/CaM-dependent phosphatase (calcineurin) is improved by mutations in URE2 or PMA1. Genetics. 1998;149:865-78 pubmed
    ..Mutations in PMA1, which encodes the yeast plasma membrane H+-ATPase, also suppress many growth defects of calcineurin mutants...
  4. Malinska K, Malinsky J, Opekarova M, Tanner W. Distribution of Can1p into stable domains reflects lateral protein segregation within the plasma membrane of living S. cerevisiae cells. J Cell Sci. 2004;117:6031-41 pubmed
    ..Finally, two other proteins were localized within the frame of the Can1p/Pma1p plasma-membrane partition. We show that Fur4p (another H+ symporter) and Sur7p (a protein of unknown function) occupy the Can1p subdomain...
  5. Mason A, Allen K, Slayman C. Effects of C-terminal truncations on trafficking of the yeast plasma membrane H+-ATPase. J Biol Chem. 2006;281:23887-98 pubmed
    ..In this study, yeast Pma1 H+-ATPase has served as a model to examine the role of the C-terminal membrane domain in ATPase stability and ..
  6. Mazón M, Eraso P, Portillo F. Efficient degradation of misfolded mutant Pma1 by endoplasmic reticulum-associated degradation requires Atg19 and the Cvt/autophagy pathway. Mol Microbiol. 2007;63:1069-77 pubmed
    ..Several mutant alleles of PMA1, the gene coding for the plasma membrane H(+)-ATPase, render misfolded proteins that are retained in the ER and ..
  7. Pizzirusso M, Chang A. Ubiquitin-mediated targeting of a mutant plasma membrane ATPase, Pma1-7, to the endosomal/vacuolar system in yeast. Mol Biol Cell. 2004;15:2401-9 pubmed
    b>Pma1-7 is a mutant plasma membrane ATPase that is impaired in targeting to the cell surface at 37 degrees C and is delivered instead to the endosomal/vacuolar pathway for degradation...
  8. Goossens A, de La Fuente N, Forment J, Serrano R, Portillo F. Regulation of yeast H(+)-ATPase by protein kinases belonging to a family dedicated to activation of plasma membrane transporters. Mol Cell Biol. 2000;20:7654-61 pubmed
    ..and HRK1 (YOR267c), that encode protein kinases implicated in activation of the yeast plasma membrane H(+)-ATPase (Pma1) in response to glucose metabolism...
  9. Liu Y, Sitaraman S, Chang A. Multiple degradation pathways for misfolded mutants of the yeast plasma membrane ATPase, Pma1. J Biol Chem. 2006;281:31457-66 pubmed
    ..control in the secretory pathway, we have analyzed intracellular trafficking of the yeast plasma membrane ATPase, Pma1. Pma1 is ideal for such studies because it is a very abundant polytopic membrane protein, and its localization and ..
  10. Nedea E, He X, Kim M, Pootoolal J, Zhong G, Canadien V, et al. Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem. 2003;278:33000-10 pubmed
    ..RNA (snoRNA) genes and primarily near the polyadenylation signals of the constitutively expressed PYK1 and PMA1 genes...
  11. Wang Q, Chang A. Substrate recognition in ER-associated degradation mediated by Eps1, a member of the protein disulfide isomerase family. EMBO J. 2003;22:3792-802 pubmed
    b>Pma1-D378N is a misfolded plasma membrane protein in yeast that is prevented from delivery to the cell surface and targeted instead for ER-associated degradation (ERAD)...
  12. Lecchi S, Nelson C, Allen K, Swaney D, Thompson K, Coon J, et al. Tandem phosphorylation of Ser-911 and Thr-912 at the C terminus of yeast plasma membrane H+-ATPase leads to glucose-dependent activation. J Biol Chem. 2007;282:35471-81 pubmed
    ..b>Pma1 H(+)-ATPase, which is responsible for H(+)-dependent nutrient uptake in yeast (Saccharomyces cerevisiae), is one ..
  13. Shia W, Osada S, Florens L, Swanson S, Washburn M, Workman J. Characterization of the yeast trimeric-SAS acetyltransferase complex. J Biol Chem. 2005;280:11987-94 pubmed
    ..This specific lysine preference corresponds to the role of SAS complex in antagonizing the spreading of Sir proteins at silent loci in S. cerevisiae. ..
  14. Vitaliano Prunier A, Babour A, Hérissant L, Apponi L, Margaritis T, Holstege F, et al. H2B ubiquitylation controls the formation of export-competent mRNP. Mol Cell. 2012;45:132-9 pubmed publisher
    ..Modification of H2B thus participates in the crosstalk between cotranscriptional events and assembly of mRNPs linking nuclear processing and mRNA export. ..
  15. Eraso P, Mazón M, Portillo F. Yeast protein kinase Ptk2 localizes at the plasma membrane and phosphorylates in vitro the C-terminal peptide of the H+-ATPase. Biochim Biophys Acta. 2006;1758:164-70 pubmed
    ..modifications that increase the activity of the Saccharomyces cerevisiae plasma membrane H+-ATPase (Pma1)...
  16. Curwin A, von Blume J, Malhotra V. Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast. Mol Biol Cell. 2012;23:2327-38 pubmed publisher
    ..was secreted at a reduced rate and retained in a late Golgi compartment, whereas the plasma membrane H(+) ATPase Pma1, which is transported in the same class of carriers, reached the cell surface...
  17. Bhat W, Boutin G, Rufiange A, Nourani A. Casein kinase 2 associates with the yeast chromatin reassembly factor Spt2/Sin1 to regulate its function in the repression of spurious transcription. Mol Cell Biol. 2013;33:4198-211 pubmed publisher
    ..Taken together, our data suggest that CK2 regulates the function of Spt2 by modulating its interaction with chromatin and the histone chaperone Spt6. ..
  18. Proft M, Struhl K. MAP kinase-mediated stress relief that precedes and regulates the timing of transcriptional induction. Cell. 2004;118:351-61 pubmed
  19. Kaminska J, Tobiasz A, Gniewosz M, Zoładek T. The growth of mdp1/rsp5 mutants of Saccharomyces cerevisiae is affected by mutations in the ATP-binding domain of the plasma membrane H+ -ATPase. Gene. 2000;242:133-40 pubmed
    Mutations in the PMA1 gene, encoding plasma membrane H+ -ATPase, were isolated that are able to suppress the temperature sensitivity (ts) phenotype of mdp1 mutations located in RSP5, the ubiquitin-protein ligase gene...
  20. Baron J, Chen J, Culotta V. Cu/Zn superoxide dismutase and the proton ATPase Pma1p of Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2015;462:251-6 pubmed publisher
    ..Surprisingly the effect of sod1Δ mutations on Pma1-T912D is not mediated through the Sod1p signaling pathway involving the CK1γ casein kinases...
  21. Usami Y, Uemura S, Mochizuki T, Morita A, Shishido F, Inokuchi J, et al. Functional mapping and implications of substrate specificity of the yeast high-affinity leucine permease Bap2. Biochim Biophys Acta. 2014;1838:1719-29 pubmed publisher
    ..This result suggests that the substrate partition efficiency to the buried Bap2 binding pocket is the primary determinant of substrate specificity rather than structural amino acid side chain recognition. ..
  22. Liu Y, Chang A. A mutant plasma membrane protein is stabilized upon loss of Yvh1, a novel ribosome assembly factor. Genetics. 2009;181:907-15 pubmed publisher
    b>Pma1-10 is a mutant plasma membrane ATPase defective at the restrictive temperature in stability at the cell surface. At 37 degrees, Pma1-10 is ubiquitinated and internalized from the plasma membrane for degradation in the vacuole...
  23. Wolf W, Kilic A, Schrul B, Lorenz H, Schwappach B, Seedorf M. Yeast Ist2 recruits the endoplasmic reticulum to the plasma membrane and creates a ribosome-free membrane microcompartment. PLoS ONE. 2012;7:e39703 pubmed publisher
    ..Ist2-dependent effects on cytosolic pH and genetic interactions link Ist2 to the activity of the H(+) pump Pma1 in the plasma membrane during cellular adaptation to the growth phase of the culture...
  24. Liu Y, Chang A. Quality control of a mutant plasma membrane ATPase: ubiquitylation prevents cell-surface stability. J Cell Sci. 2006;119:360-9 pubmed
    The plasma membrane ATPase, Pma1, has remarkable longevity at the cell surface...
  25. Petrov V. Role of loop L5-6 connecting transmembrane segments M5 and M6 in biogenesis and functioning of yeast Pma1 H+-ATPase. Biochemistry (Mosc). 2015;80:31-44 pubmed publisher
    ..To study structure-function relationships within this loop of the yeast plasma membrane Pma1 H+-ATPase, alanine- and cysteine-scanning mutagenesis has been employed...
  26. Souza A, Miranda M, da Silva S, Bozaquel Morais B, Masuda C, Ghislain M, et al. Expression of the glucose transporter HXT1 involves the Ser-Thr protein phosphatase Sit4 in Saccharomyces cerevisiae. FEMS Yeast Res. 2012;12:907-17 pubmed publisher
    ..It was concluded that Sit4p ensures that HXT1 transcript is efficiently transcribed and translated thus increasing protein levels of Hxt1p when high glucose levels are present. ..
  27. Oblak E, Piecuch A, Maciaszczyk Dziubinska E, Wawrzycka D. Quaternary ammonium salt N-(dodecyloxycarboxymethyl)-N,N,N-trimethyl ammonium chloride induced alterations in Saccharomyces cerevisiae physiology. J Biosci. 2016;41:601-614 pubmed
    ..These results indicate changes in the cell lipid composition. Western blot analysis showed the induction of Pma1 level by IM. RT-PCR revealed an increased PMA1 expression after IM treatment.
  28. Peters L, Hazan R, Breker M, Schuldiner M, Ben Aroya S. Formation and dissociation of proteasome storage granules are regulated by cytosolic pH. J Cell Biol. 2013;201:663-71 pubmed publisher
    ..We thus identify cytosolic pH as a specific cellular signal involved both in the glucose sensing that mediates PSG formation and in a more general mechanism for signaling carbon source exhaustion. ..
  29. Supply P, Wach A, Goffeau A. Enzymatic properties of the PMA2 plasma membrane-bound H(+)-ATPase of Saccharomyces cerevisiae. J Biol Chem. 1993;268:19753-9 pubmed
    The PMA1 H(+)-ATPase can be functionally replaced by its isoform PMA2 in the plasma membrane from Saccharomyces cerevisiae (Supply, P., Wach, A., Thinès-Sempoux, D., and Goffeau, A. (1993) J. Biol. Chem. 268, 19744-19752)...
  30. Navarre C, Degand H, Bennett K, Crawford J, Mørtz E, Boutry M. Subproteomics: identification of plasma membrane proteins from the yeast Saccharomyces cerevisiae. Proteomics. 2002;2:1706-14 pubmed
    ..Thus, combining subcellular fractionation with adapted two-dimensional electrophoresis resulted in the identification of intrinsic plasma membrane proteins. ..
  31. Fraysse A, Møller A, Poulsen L, Wollenweber B, Buch Pedersen M, Palmgren M. A systematic mutagenesis study of Ile-282 in transmembrane segment M4 of the plasma membrane H+-ATPase. J Biol Chem. 2005;280:21785-90 pubmed
    ..The ability of mutant enzymes to substitute for the yeast proton pump PMA1 was found to correlate with the size of the side chain rather than its chemical nature...
  32. Petrov V. Functioning of Yeast Pma1 H+-ATPase under Changing Charge: Role of Asp739 and Arg811 Residues. Biochemistry (Mosc). 2017;82:46-59 pubmed publisher
    The plasma membrane Pma1 H+-ATPase of the yeast Saccharomyces cerevisiae contains conserved residue Asp739 located at the interface of transmembrane segment M6 and the cytosol. Its replacement by Asn or Val (Petrov et al. (2000) J. Biol...
  33. Safiarian M, Pertl Obermeyer H, Lughofer P, Hude R, Bertl A, Obermeyer G. Lost in traffic? The K(+) channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves, and lily pollen. Front Plant Sci. 2015;6:47 pubmed publisher
  34. Soares L, Buratowski S. Yeast Swd2 is essential because of antagonism between Set1 histone methyltransferase complex and APT (associated with Pta1) termination factor. J Biol Chem. 2012;287:15219-31 pubmed publisher
    ..Although Swd2 is normally required for bringing APT to genes, deletion of SET1 restores both viability and APT recruitment to a strain lacking Swd2. We propose a model in which Swd2 is required for APT to overcome antagonism by COMPASS. ..
  35. Luna R, Jimeno S, Marin M, Huertas P, Garcia Rubio M, Aguilera A. Interdependence between transcription and mRNP processing and export, and its impact on genetic stability. Mol Cell. 2005;18:711-22 pubmed
    ..The results suggest a tight interdependence among mRNP biogenesis steps and transcription and an unexpected effect of the nuclear exosome and the cleavage/polyadenylation factors on transcription elongation and genetic integrity. ..
  36. Surma M, Klose C, Klemm R, Ejsing C, Simons K. Generic sorting of raft lipids into secretory vesicles in yeast. Traffic. 2011;12:1139-47 pubmed publisher
    ..This finding indicates that lipid raft sorting is a generic feature of vesicles carrying PM cargo and suggests a common lipid-based mechanism for their formation...
  37. Mazón M, Eraso P, Portillo F. Specific phosphoantibodies reveal two phosphorylation sites in yeast Pma1 in response to glucose. FEMS Yeast Res. 2015;15:fov030 pubmed publisher
    Glucose triggers post-translational modifications of the Saccharomyces cerevisiae plasma membrane H(+)-ATPase (Pma1) that lead to an increase in enzyme activity...
  38. Smardon A, Kane P. Loss of vacuolar H+-ATPase activity in organelles signals ubiquitination and endocytosis of the yeast plasma membrane proton pump Pma1p. J Biol Chem. 2014;289:32316-26 pubmed publisher
    ..We hypothesize that loss of V-ATPase-mediated organelle acidification signals ubiquitination, internalization, and degradation of a portion of Pma1p as a means of balancing overall pH homeostasis. ..
  39. Zhang L, Schroeder S, Fong N, Bentley D. Altered nucleosome occupancy and histone H3K4 methylation in response to 'transcriptional stress'. EMBO J. 2005;24:2379-90 pubmed
    ..This response identifies a new role for H3K4 trimethylation at the 3' end of the gene, as a chromatin mark associated with impaired pol II transcription. ..
  40. Calvo O, Manley J. The transcriptional coactivator PC4/Sub1 has multiple functions in RNA polymerase II transcription. EMBO J. 2005;24:1009-20 pubmed
    ..Our data provide evidence that Rna15 and Sub1 are present along the length of several genes and that Sub1 facilitates elongation by influencing enzymes that modify RNAP II. ..
  41. Petrov V. Point mutations in the extracytosolic loop between transmembrane segments M5 and M6 of the yeast Pma1 H+-ATPase: alanine-scanning mutagenesis. J Biomol Struct Dyn. 2015;33:70-84 pubmed publisher
    ..mutagenesis to explore the structural and functional relationships within this loop of the yeast plasma membrane Pma1 H(+)-ATPase...
  42. Uemura S, Shishido F, Tani M, Mochizuki T, Abe F, Inokuchi J. Loss of hydroxyl groups from the ceramide moiety can modify the lateral diffusion of membrane proteins in S. cerevisiae. J Lipid Res. 2014;55:1343-56 pubmed publisher
    ..These results suggest that C4-OH and C2-OH are most probably critical factors for maintaining membrane fluidity and proper turnover of membrane molecules in yeast containing complex sphingolipids with only one hydrophilic head group. ..
  43. Mason A, Allen K, Slayman C. C-terminal truncations of the Saccharomyces cerevisiae PMA1 H+-ATPase have major impacts on protein conformation, trafficking, quality control, and function. Eukaryot Cell. 2014;13:43-52 pubmed publisher
    ..Thus, the C terminus is much more than a simple appendage and profoundly influences the structure, biogenesis, and function of the yeast H(+)-ATPase. ..
  44. Luo W, Gong X, Chang A. An ER membrane protein, Sop4, facilitates ER export of the yeast plasma membrane [H+]ATPase, Pma1. Traffic. 2002;3:730-9 pubmed
    ..the mechanism by which Sop4, a novel ER membrane protein, regulates quality control and intracellular transport of Pma1-7, a mutant plasma membrane ATPase...
  45. Eraso P, Portillo F, Mazón M. Characterization of two second-site mutations preventing wild type protein aggregation caused by a dominant negative PMA1 mutant. PLoS ONE. 2013;8:e67080 pubmed publisher
    The correct biogenesis and localization of Pma1 at the plasma membrane is essential for yeast growth...
  46. Petrov V. Point mutations in Pma1 H+-ATPase of Saccharomyces cerevisiae: influence on its expression and activity. Biochemistry (Mosc). 2010;75:1055-63 pubmed
    Yeast Pma1 H+-ATPase is a key enzyme of cell metabolism generating electrochemical proton gradient across the plasma membrane, thus playing an important role in the maintenance of ion homeostasis in the cell...
  47. Ayciriex S, Le Guedard M, Camougrand N, Velours G, Schoene M, Leone S, et al. YPR139c/LOA1 encodes a novel lysophosphatidic acid acyltransferase associated with lipid droplets and involved in TAG homeostasis. Mol Biol Cell. 2012;23:233-46 pubmed publisher
    ..Our results from purification of subcellular compartments and proteomic studies show that Loa1p is associated with LD and active in this compartment. Loa1p is therefore a novel LPA AT and plays a role in LD formation. ..
  48. Parreiras L, Kohn L, Anderson J. Cellular effects and epistasis among three determinants of adaptation in experimental populations of Saccharomyces cerevisiae. Eukaryot Cell. 2011;10:1348-56 pubmed publisher
    ..These data show how positive and negative epistasis can contribute to adaptation and reproductive isolation. ..
  49. Petrov V, Ibragimov R. [Effect of point substitutions of Asp-714 and Asp-720 residues on the structure and function of the H+ -ATPase of the yeast plasma membrane]. Prikl Biokhim Mikrobiol. 2014;50:508-16 pubmed
    ..The results indicate the important role for the Asp-714 residue in biogenesis, structure stability, and enzyme function. ..
  50. Shimoni Y, Kurihara T, Ravazzola M, Amherdt M, Orci L, Schekman R. Lst1p and Sec24p cooperate in sorting of the plasma membrane ATPase into COPII vesicles in Saccharomyces cerevisiae. J Cell Biol. 2000;151:973-84 pubmed
    ..By changing the geometry of COPII coat polymerization, Lst1p may allow the transport of bulky cargo molecules, polymers, or particles...
  51. Binda M, Péli Gulli M, Bonfils G, Panchaud N, Urban J, Sturgill T, et al. The Vam6 GEF controls TORC1 by activating the EGO complex. Mol Cell. 2009;35:563-73 pubmed publisher
    ..Thus, in addition to its regulatory role in homotypic vacuolar fusion and vacuole protein sorting within the HOPS complex, Vam6 also controls TORC1 function by activating the Gtr1 subunit of the EGO complex. ..
  52. Daniel J. Direct in vivo access to potential gene targets of the RPD3 histone deactylase using fitness-based interferential genetics. Yeast. 2007;24:575-87 pubmed
    ..Consistent with this, FIG selection also retrieved: the PMA1 gene, encoding the plasma H(+)-membrane ATPase; FOL2 and FOL3, involved in folic acid biosynthesis; and UBR2, which ..
  53. Alder Baerens N, Lisman Q, Luong L, Pomorski T, Holthuis J. Loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles. Mol Biol Cell. 2006;17:1632-42 pubmed
    ..Our results indicate that aminophospholipid asymmetry is created when membrane flows through the Golgi and that P4-ATPases are essential for this process. ..
  54. Williams Hart T, Wu X, Tatchell K. Protein phosphatase type 1 regulates ion homeostasis in Saccharomyces cerevisiae. Genetics. 2002;160:1423-37 pubmed
    ..The CsCl sensitivity of this mutant is suppressed by recessive mutations in PMA1, which encodes the essential plasma membrane H(+)ATPase...
  55. DERMODY J, Buratowski S. Leo1 subunit of the yeast paf1 complex binds RNA and contributes to complex recruitment. J Biol Chem. 2010;285:33671-9 pubmed publisher
    ..Together, these results suggest that association of Paf1C with RNA stabilizes its localization at actively transcribed regions where it influences chromatin structure. ..
  56. Dechant R, Saad S, Ibáñez A, Peter M. Cytosolic pH regulates cell growth through distinct GTPases, Arf1 and Gtr1, to promote Ras/PKA and TORC1 activity. Mol Cell. 2014;55:409-21 pubmed publisher
    ..Together, these data provide a molecular mechanism for how cytosolic pH links C-source availability to the activity of signaling networks promoting cell growth. ..
  57. Lauwers E, Jacob C, Andre B. K63-linked ubiquitin chains as a specific signal for protein sorting into the multivesicular body pathway. J Cell Biol. 2009;185:493-502 pubmed publisher
    ..Our data reveal that K63-linked Ub chains act as a specific signal for MVB sorting, providing further insight into the Ub code of membrane protein trafficking. ..
  58. Luo W, Chang A. Novel genes involved in endosomal traffic in yeast revealed by suppression of a targeting-defective plasma membrane ATPase mutant. J Cell Biol. 1997;138:731-46 pubmed
    ..We took advantage of a temperature-sensitive mutant in PMA1, encoding the plasma membrane ATPase, in which newly synthesized Pma1 is mislocalized to the vacuole via the ..
  59. Yoshimura H, Tada T, Iida H. Subcellular localization and oligomeric structure of the yeast putative stretch-activated Ca2+ channel component Mid1. Exp Cell Res. 2004;293:185-95 pubmed
    ..These findings provide new insight into the function of Mid1 in relation to localization, modification, and activation mechanisms. ..
  60. Lee Y, Nasution O, Lee Y, Kim E, Choi W, Kim W. Overexpression of PMA1 enhances tolerance to various types of stress and constitutively activates the SAPK pathways in Saccharomyces cerevisiae. Appl Microbiol Biotechnol. 2017;101:229-239 pubmed publisher
    b>PMA1 encodes a transmembrane polypeptide that functions to pump protons out of the cell...
  61. Deng Y, Guo Y, Watson H, Au W, Shakoury Elizeh M, Basrai M, et al. Gga2 mediates sequential ubiquitin-independent and ubiquitin-dependent steps in the trafficking of ARN1 from the trans-Golgi network to the vacuole. J Biol Chem. 2009;284:23830-41 pubmed publisher
    ..These studies suggest that Gga2 is involved in sorting at both the TGN and multivesicular body and that the first step can occur without ubiquitin binding. ..