Gene Symbol: PKH2
Description: serine/threonine protein kinase PKH2
Alias: serine/threonine protein kinase PKH2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Casamayor A, Torrance P, Kobayashi T, Thorner J, Alessi D. Functional counterparts of mammalian protein kinases PDK1 and SGK in budding yeast. Curr Biol. 1999;9:186-97 pubmed
    ..We found that two previously uncharacterised genes of Saccharomyces cerevisiae, which we term PKH1 and PKH2, encode protein kinases with catalytic domains closely resembling those of human and Drosophila PDK1...
  2. deHart A, Schnell J, Allen D, Hicke L. The conserved Pkh-Ypk kinase cascade is required for endocytosis in yeast. J Cell Biol. 2002;156:241-8 pubmed
    ..These observations demonstrate that Ypk1 acts downstream of the Pkh kinases to control endocytosis by phosphorylating components of the endocytic machinery. ..
  3. Daquinag A, Fadri M, Jung S, Qin J, Kunz J. The yeast PH domain proteins Slm1 and Slm2 are targets of sphingolipid signaling during the response to heat stress. Mol Cell Biol. 2007;27:633-50 pubmed a phosphorylation/dephosphorylation cycle involving the sphingolipid-activated protein kinases Pkh1 and Pkh2 and the calcium/calmodulin-dependent protein phosphatase calcineurin...
  4. Walther T, Aguilar P, Fröhlich F, Chu F, Moreira K, Burlingame A, et al. Pkh-kinases control eisosome assembly and organization. EMBO J. 2007;26:4946-55 pubmed
    ..more Pil1 assembles into eisosomes if Pil1 is hypophosphorylated (i) upon reducing Pkh-kinase activity in pkh1 pkh2-mutant cells, (ii) upon activating Pkh-kinases by addition of LCBs, and (iii) upon mimicking hypophosphorylation in ..
  5. Huang X, Liu J, Dickson R. Down-regulating sphingolipid synthesis increases yeast lifespan. PLoS Genet. 2012;8:e1002493 pubmed publisher
    ..Sphingolipids are found in all eukaryotes and our results suggest that pharmacological down-regulation of one or more sphingolipids may provide a means to reduce age-related diseases and increase lifespan in other eukaryotes. ..
  6. Roelants F, Torrance P, Bezman N, Thorner J. Pkh1 and Pkh2 differentially phosphorylate and activate Ypk1 and Ykr2 and define protein kinase modules required for maintenance of cell wall integrity. Mol Biol Cell. 2002;13:3005-28 pubmed
    Saccharomyces cerevisiae Pkh1 and Pkh2 are functionally redundant homologs of mammalian protein kinase, phosphoinositide-dependent protein kinase-1...
  7. Inagaki M, Schmelzle T, Yamaguchi K, Irie K, Hall M, Matsumoto K. PDK1 homologs activate the Pkc1-mitogen-activated protein kinase pathway in yeast. Mol Cell Biol. 1999;19:8344-52 pubmed
    ..Using a genetic selection based on a mutant form of the yeast MAP kinase kinase Ste7, we isolated a gene, PKH2, encoding a structurally and functionally conserved yeast homolog of PDK1...
  8. Zhang X, Lester R, Dickson R. Pil1p and Lsp1p negatively regulate the 3-phosphoinositide-dependent protein kinase-like kinase Pkh1p and downstream signaling pathways Pkc1p and Ypk1p. J Biol Chem. 2004;279:22030-8 pubmed
    ..Homologs of Pil1p and Lsp1p are widespread in nature, and our results suggest that they may be negative regulators of PDK-like protein kinases and their downstream cellular pathways that control cell growth and survival. ..
  9. Grosshans B, Grötsch H, Mukhopadhyay D, Fernández I, Pfannstiel J, Idrissi F, et al. TEDS site phosphorylation of the yeast myosins I is required for ligand-induced but not for constitutive endocytosis of the G protein-coupled receptor Ste2p. J Biol Chem. 2006;281:11104-14 pubmed

More Information


  1. Luo G, Costanzo M, Boone C, Dickson R. Nutrients and the Pkh1/2 and Pkc1 protein kinases control mRNA decay and P-body assembly in yeast. J Biol Chem. 2011;286:8759-70 pubmed publisher
    ..Here we show that a signal transduction pathway containing the Pkh1/Pkh2 protein kinases and one of their effector kinases, Pkc1, is required for and regulates global mRNA decay at the ..
  2. Sun Y, Miao Y, Yamane Y, Zhang C, Shokat K, Takematsu H, et al. Orm protein phosphoregulation mediates transient sphingolipid biosynthesis response to heat stress via the Pkh-Ypk and Cdc55-PP2A pathways. Mol Biol Cell. 2012;23:2388-98 pubmed publisher
    ..We propose that this mechanism serves as the basis for how Orm phosphoregulation controls sphingolipid biosynthesis in response to stress in a kinetically coupled manner. ..
  3. Omnus D, Manford A, Bader J, Emr S, Stefan C. Phosphoinositide kinase signaling controls ER-PM cross-talk. Mol Biol Cell. 2016;27:1170-80 pubmed publisher
    ..Thus PI kinase-mediated ER-PM cross-talk comprises a regulatory system that ensures cellular integrity under membrane stress conditions. ..
  4. González A, Shimobayashi M, Eisenberg T, Merle D, Pendl T, Hall M, et al. TORC1 promotes phosphorylation of ribosomal protein S6 via the AGC kinase Ypk3 in Saccharomyces cerevisiae. PLoS ONE. 2015;10:e0120250 pubmed publisher
    ..Our findings demonstrate that Ypk3 is a critical component of the TORC1 pathway and that the use of a phospho-S6 specific antibody offers a valuable tool to identify new nutrient-dependent and rapamycin-sensitive targets in vivo. ..
  5. Pohlers S, Martin R, Krüger T, Hellwig D, Hänel F, Kniemeyer O, et al. Lipid Signaling via Pkh1/2 Regulates Fungal CO2 Sensing through the Kinase Sch9. MBio. 2017;8: pubmed publisher
    ..Furthermore, we provide a direct link between adaptation to changing CO2 conditions and lipid/Pkh1/2 signaling in yeast, thus establishing a new signaling cascade central to metabolic adaptation. ..
  6. Mirisola M, Taormina G, Fabrizio P, Wei M, Hu J, Longo V. Serine- and threonine/valine-dependent activation of PDK and Tor orthologs converge on Sch9 to promote aging. PLoS Genet. 2014;10:e1004113 pubmed publisher
    ..Because of the conserved pro-aging role of nutrient and growth signaling pathways in higher eukaryotes, these results raise the possibility that similar mechanisms contribute to aging in mammals...
  7. Morvan J, Rinaldi B, Friant S. Pkh1/2-dependent phosphorylation of Vps27 regulates ESCRT-I recruitment to endosomes. Mol Biol Cell. 2012;23:4054-64 pubmed publisher
    ..We propose that Vps27 phosphorylation by Pkh1/2 kinases regulates the coordinated cascade of ESCRT complex recruitment at the endosomal membrane. ..
  8. Luo G, Gruhler A, Liu Y, Jensen O, Dickson R. The sphingolipid long-chain base-Pkh1/2-Ypk1/2 signaling pathway regulates eisosome assembly and turnover. J Biol Chem. 2008;283:10433-44 pubmed publisher
    ..studies showed that these proteins are phosphorylated by the sphingolipid long-chain base-activated Pkh1 and Pkh2 protein kinases in vitro...