Gene Symbol: PKC1
Description: protein kinase C
Alias: CLY15, CLY5, CLY7, HPO2, STT1, protein kinase C
Species: Saccharomyces cerevisiae S288c
Products:     PKC1

Top Publications

  1. Lee K, Levin D. Dominant mutations in a gene encoding a putative protein kinase (BCK1) bypass the requirement for a Saccharomyces cerevisiae protein kinase C homolog. Mol Cell Biol. 1992;12:172-82 pubmed
    The PKC1 gene of Saccharomyces cerevisiae encodes a homolog of mammalian protein kinase C that is required for yeast cell growth and division...
  2. Mascaraque V, Hernaez M, Jimenez Sanchez M, Hansen R, Gil C, Martín H, et al. Phosphoproteomic analysis of protein kinase C signaling in Saccharomyces cerevisiae reveals Slt2 mitogen-activated protein kinase (MAPK)-dependent phosphorylation of eisosome core components. Mol Cell Proteomics. 2013;12:557-74 pubmed publisher activated under a variety of stimuli related to cell wall homeostasis by Pkc1, the only member of the protein kinase C family in budding yeast...
  3. Vilella F, Herrero E, Torres J, De La Torre Ruiz M. Pkc1 and the upstream elements of the cell integrity pathway in Saccharomyces cerevisiae, Rom2 and Mtl1, are required for cellular responses to oxidative stress. J Biol Chem. 2005;280:9149-59 pubmed
    In this study we analyze the participation of the PKC1-MAPK cell integrity pathway in cellular responses to oxidative stress in Saccharomyces cerevisiae...
  4. Ketela T, Green R, Bussey H. Saccharomyces cerevisiae mid2p is a potential cell wall stress sensor and upstream activator of the PKC1-MPK1 cell integrity pathway. J Bacteriol. 1999;181:3330-40 pubmed
    ..mid2Delta mutants can be suppressed by overexpression of upstream elements of the cell integrity pathway, including PKC1, RHO1, WSC1, and WSC2...
  5. Yasutis K, Vignali M, Ryder M, Tameire F, Dighe S, Fields S, et al. Zds2p regulates Swe1p-dependent polarized cell growth in Saccharomyces cerevisiae via a novel Cdc55p interaction domain. Mol Biol Cell. 2010;21:4373-86 pubmed publisher
    ..This negative regulation requires the CDC55 gene. Together these data indicate that the Cdc55p/Zds2p module has a function in the regulation of the Swe1p-dependent G2/M checkpoint. ..
  6. Irie K, Takase M, Lee K, Levin D, Araki H, Matsumoto K, et al. MKK1 and MKK2, which encode Saccharomyces cerevisiae mitogen-activated protein kinase-kinase homologs, function in the pathway mediated by protein kinase C. Mol Cell Biol. 1993;13:3076-83 pubmed
    The PKC1 gene of Saccharomyces cerevisiae encodes a homolog of mammalian protein kinase C that is required for normal growth and division of yeast cells...
  7. Kono K, Nogami S, Abe M, Nishizawa M, Morishita S, Pellman D, et al. G1/S cyclin-dependent kinase regulates small GTPase Rho1p through phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiae. Mol Biol Cell. 2008;19:1763-71 pubmed publisher
    ..We propose that Cln2p/Cdc28p-dependent phosphorylation of Tus1p is required for appropriate temporal and spatial activation of Rho1p at the G1/S transition. ..
  8. Queralt E, Igual J. Functional connection between the Clb5 cyclin, the protein kinase C pathway and the Swi4 transcription factor in Saccharomyces cerevisiae. Genetics. 2005;171:1485-98 pubmed
    ..Interestingly, genes of the PKC pathway rescue the growth defect of clb5 swi4, and pkc1 and slt2 mutations are synthetic lethal with clb5, pointing to a connection between Clb5, the PKC pathway, and Swi4...
  9. Yang W, Bruno M, Carman G. Regulation of yeast CTP synthetase activity by protein kinase C. J Biol Chem. 1996;271:11113-9 pubmed
    ..In this work we examined the regulation of CTP synthetase activity by S. cerevisiae protein kinase C (Pkc1p) phosphorylation. The results of labeling experiments with S...

More Information


  1. Inagaki M, Schmelzle T, Yamaguchi K, Irie K, Hall M, Matsumoto K. PDK1 homologs activate the Pkc1-mitogen-activated protein kinase pathway in yeast. Mol Cell Biol. 1999;19:8344-52 pubmed
    ..A temperature-sensitive mutant, pkh1(D398G) pkh2, was phenotypically similar to mutants defective in the Pkc1-mitogen-activated protein kinase (MAPK) pathway...
  2. Igual J, Johnson A, Johnston L. Coordinated regulation of gene expression by the cell cycle transcription factor Swi4 and the protein kinase C MAP kinase pathway for yeast cell integrity. EMBO J. 1996;15:5001-13 pubmed
    ..rsf8 is allelic to PKC1, encoding a protein kinase C homologue which controls cell integrity...
  3. Huang K, Symington L. Suppressors of a Saccharomyces cerevisiae pkc1 mutation identify alleles of the phosphatase gene PTC1 and of a novel gene encoding a putative basic leucine zipper protein. Genetics. 1995;141:1275-85 pubmed
    The PKC1 gene product, protein kinase C, regulates a mitogen-activated protein kinase (MAPK) cascade, which is implicated in cell wall metabolism...
  4. Watanabe M, Chen C, Levin D. Saccharomyces cerevisiae PKC1 encodes a protein kinase C (PKC) homolog with a substrate specificity similar to that of mammalian PKC. J Biol Chem. 1994;269:16829-36 pubmed
    ..budding yeast Saccharomyces cerevisiae encodes a homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) that is essential for cell growth...
  5. Roelants F, Torrance P, Thorner J. Differential roles of PDK1- and PDK2-phosphorylation sites in the yeast AGC kinases Ypk1, Pkc1 and Sch9. Microbiology. 2004;150:3289-304 pubmed
    ..A fourth protein kinase involved in growth control and stress response, Sch9 (orthologue of mammalian protein kinase c-Akt/PKB), also carries the conserved activation loop motif...
  6. Nonaka H, Tanaka K, Hirano H, Fujiwara T, Kohno H, Umikawa M, et al. A downstream target of RHO1 small GTP-binding protein is PKC1, a homolog of protein kinase C, which leads to activation of the MAP kinase cascade in Saccharomyces cerevisiae. EMBO J. 1995;14:5931-8 pubmed
    ..gene revealed that the mutation occurred at the pseuodosubstrate site of PKC1, a yeast homolog of mammalian protein kinase C. Two-hybrid analysis demonstrated that GTP-Rho1p, but not GDP-Rho1p, interacted with the region of Pkc1p ..
  7. Gray J, Ogas J, Kamada Y, Stone M, Levin D, Herskowitz I. A role for the Pkc1 MAP kinase pathway of Saccharomyces cerevisiae in bud emergence and identification of a putative upstream regulator. EMBO J. 1997;16:4924-37 pubmed
    The protein kinase C of Saccharomyces cerevisiae, Pkc1, regulates a MAP kinase, Mpk1, whose activity is stimulated at the G1-S transition of the cell cycle and by perturbations to the cell surface, e.g. induced by heat shock...
  8. Helliwell S, Howald I, Barbet N, Hall M. TOR2 is part of two related signaling pathways coordinating cell growth in Saccharomyces cerevisiae. Genetics. 1998;148:99-112 pubmed
    ..Overexpression of MSS4, PKC1, PLC1, RHO2, ROM2, or SUR1 suppressed the growth defect of a class A mutant...
  9. Andrews P, Stark M. Dynamic, Rho1p-dependent localization of Pkc1p to sites of polarized growth. J Cell Sci. 2000;113 ( Pt 15):2685-93 pubmed
    ..Budding yeast Pkc1p, a protein kinase C-like enzyme involved in cell wall biosynthesis and cytoskeletal polarity, is structurally and functionally ..
  10. Leduc A, He C, Ramotar D. Disruption of the Saccharomyces cerevisiae cell-wall pathway gene SLG1 causes hypersensitivity to the antitumor drug bleomycin. Mol Genet Genomics. 2003;269:78-89 pubmed
    ..membrane protein that senses cell wall stress, and functions to maintain cell wall function by activating the protein kinase C signaling pathway...
  11. Denis V, Cyert M. Molecular analysis reveals localization of Saccharomyces cerevisiae protein kinase C to sites of polarized growth and Pkc1p targeting to the nucleus and mitotic spindle. Eukaryot Cell. 2005;4:36-45 pubmed
    The catalytic activity and intracellular localization of protein kinase C (PKC) are both highly regulated in vivo. This family of kinases contains conserved regulatory motifs, i.e...
  12. Nanduri J, Tartakoff A. The arrest of secretion response in yeast: signaling from the secretory path to the nucleus via Wsc proteins and Pkc1p. Mol Cell. 2001;8:281-9 pubmed
    ..The activity of the downstream kinase, Pkc1p, is also required; however, the Pkc1p MAP kinase cascade is not. sec mutants initiate compensatory transcriptional changes distinct from those of the unfolded protein response. ..
  13. Zanelli C, Valentini S. Pkc1 acts through Zds1 and Gic1 to suppress growth and cell polarity defects of a yeast eIF5A mutant. Genetics. 2005;171:1571-81 pubmed
    ..We have previously shown that high-copy PKC1 suppresses the phenotype of tif51A-1, a temperature-sensitive mutant of eIF5A in S. cerevisiae...
  14. Schmelzle T, Helliwell S, Hall M. Yeast protein kinases and the RHO1 exchange factor TUS1 are novel components of the cell integrity pathway in yeast. Mol Cell Biol. 2002;22:1329-39 pubmed
    The PKC1-associated mitogen-activated protein (MAP) kinase pathway of Saccharomyces cerevisiae regulates cell integrity by controlling the actin cytoskeleton and cell wall synthesis...
  15. Yoshida S, Bartolini S, Pellman D. Mechanisms for concentrating Rho1 during cytokinesis. Genes Dev. 2009;23:810-23 pubmed publisher
    ..These findings comprehensively define the targeting mechanisms of Rho1 essential for cytokinesis in yeast, and are likely to be relevant to cytokinesis in other organisms. ..
  16. Helliwell S, Schmidt A, Ohya Y, Hall M. The Rho1 effector Pkc1, but not Bni1, mediates signalling from Tor2 to the actin cytoskeleton. Curr Biol. 1998;8:1211-4 pubmed
    ..Four Rho1 effectors are known, protein kinase C 1 (Pkc1), the formin-family protein Bni1, the glucan synthase Fks and the signalling protein Skn7 [2,3]...
  17. Kono K, Saeki Y, Yoshida S, Tanaka K, Pellman D. Proteasomal degradation resolves competition between cell polarization and cellular wound healing. Cell. 2012;150:151-64 pubmed publisher
    ..Mechanisms to overcome competition from a pre-existing polarized cytoskeleton may be a general feature of effective wound healing in polarized cells. ..
  18. Fujiwara T, Tanaka K, Mino A, Kikyo M, Takahashi K, Shimizu K, et al. Rho1p-Bni1p-Spa2p interactions: implication in localization of Bni1p at the bud site and regulation of the actin cytoskeleton in Saccharomyces cerevisiae. Mol Biol Cell. 1998;9:1221-33 pubmed
    ..These results indicate that both Rho1p and Spa2p are involved in the localization of Bni1p at the growth sites where Rho1p regulates reorganization of the actin cytoskeleton through Bni1p. ..
  19. Kamada Y, Qadota H, Python C, Anraku Y, Ohya Y, Levin D. Activation of yeast protein kinase C by Rho1 GTPase. J Biol Chem. 1996;271:9193-6 pubmed
    ..a cell lysis defect that is similar to that of mutants in the cell integrity signaling pathway mediated by protein kinase C (Pkc1), which is suppressed by overexpression of Pkc1...
  20. Levin D, Bowers B, Chen C, Kamada Y, Watanabe M. Dissecting the protein kinase C/MAP kinase signalling pathway of Saccharomyces cerevisiae. Cell Mol Biol Res. 1994;40:229-39 pubmed
    The PKC1 gene of the budding yeast Saccharomyces cerevisiae encodes a homolog of the alpha, beta, and gamma isoforms of mammalian PKC that is essential for cell growth...
  21. Verna J, Lodder A, Lee K, Vagts A, Ballester R. A family of genes required for maintenance of cell wall integrity and for the stress response in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1997;94:13804-9 pubmed
    The PKC1-MPK1 pathway in yeast functions in the maintenance of cell wall integrity and in the stress response. We have identified a family of genes that are putative regulators of this pathway...
  22. Madden K, Sheu Y, Baetz K, Andrews B, Snyder M. SBF cell cycle regulator as a target of the yeast PKC-MAP kinase pathway. Science. 1997;275:1781-4 pubmed
    b>Protein kinase C (PKC) signaling is highly conserved among eukaryotes and has been implicated in the regulation of cellular processes such as cell proliferation and growth...
  23. Hsieh L, Su W, Han G, Carman G. Phosphorylation of Yeast Pah1 Phosphatidate Phosphatase by Casein Kinase II Regulates Its Function in Lipid Metabolism. J Biol Chem. 2016;291:9974-90 pubmed publisher
    ..The prephosphorylation of Pah1 by protein kinase A or protein kinase C reduced its subsequent phosphorylation by CKII...
  24. Salgado A, Schuller D, Casal M, Leao C, Leiper F, Carling D, et al. Relationship between protein kinase C and derepression of different enzymes. FEBS Lett. 2002;532:324-32 pubmed
    The PKC1 gene in the yeast Saccharomyces cerevisiae encodes for protein kinase C which is known to control a MAP kinase cascade consisting of different kinases: Bck1, Mkk1 and Mkk2, and Mpk1...
  25. Thai V, Dephoure N, Weiss A, Ferguson J, Leitao R, Gygi S, et al. Protein Kinase C Controls Binding of Igo/ENSA Proteins to Protein Phosphatase 2A in Budding Yeast. J Biol Chem. 2017;292:4925-4941 pubmed publisher
    ..b>Protein kinase C (Pkc1) relays signals in the pathway by activating PP2ACdc55 We discovered that constitutively ..
  26. Nita Lazar M, Lennarz W. Pkc1p modifies CPY* degradation in the ERAD pathway. Biochem Biophys Res Commun. 2005;332:357-61 pubmed
    ..Pkc1p has been identified as a component of the ERAD pathway, because deletion of the pkc1 gene impairs ERAD and causes accumulation of CPY* in the lumen of the ER, most probably because of the ..
  27. Chapa y Lazo B, Ayscough K. Apm4, the mu subunit of yeast AP-2 interacts with Pkc1, and mutation of the Pkc1 consensus phosphorylation site Thr176 inhibits AP-2 recruitment to endocytic sites. Commun Integr Biol. 2014;7:e28522 pubmed publisher
    ..Here we demonstrate an interaction between Apm4 and the yeast cell wall integrity pathway component Pkc1 and show that mutation of the predicted Pkc1 site in the Apm4 hinge region affects recruitment of the AP-2 complex ..
  28. Jacoby J, Nilius S, Heinisch J. A screen for upstream components of the yeast protein kinase C signal transduction pathway identifies the product of the SLG1 gene. Mol Gen Genet. 1998;258:148-55 pubmed caffeine can be partially suppressed by overexpression of genes for other components of the Pkc pathway, such as PKC1, SLT2, ROM2, and STE20...
  29. Onishi M, Ko N, Nishihama R, Pringle J. Distinct roles of Rho1, Cdc42, and Cyk3 in septum formation and abscission during yeast cytokinesis. J Cell Biol. 2013;202:311-29 pubmed publisher
    ..This work suggests a general role for the catalytically inactive transglutaminases of fungi and animals, some of which have previously been implicated in cytokinesis. ..
  30. Hsieh M, Chen R. Cdc48 and cofactors Npl4-Ufd1 are important for G1 progression during heat stress by maintaining cell wall integrity in Saccharomyces cerevisiae. PLoS ONE. 2011;6:e18988 pubmed publisher
    ..Thus, Cdc48-Npl4-Ufd1 is important for the maintenance of cell wall integrity in order for normal cell growth and division. ..
  31. Mouchel N, Jenkins J. The identification of a functional interaction between PKC and topoisomerase II. FEBS Lett. 2006;580:51-7 pubmed
    ..We identified an interaction between the catalytic domain of the yeast protein kinase 1 enzyme (Pkc1) and the N-terminal domain of the S. cerevisiae topoisomerase II. The S...
  32. Darieva Z, Webber A, Warwood S, Sharrocks A. Protein kinase C coordinates histone H3 phosphorylation and acetylation. elife. 2015;4:e09886 pubmed publisher
    ..Here we have identified the protein kinase Pkc1 as a key regulator that coordinates the deposition of these modifications in S...
  33. Garrett Engele P, Moilanen B, Cyert M. Calcineurin, the Ca2+/calmodulin-dependent protein phosphatase, is essential in yeast mutants with cell integrity defects and in mutants that lack a functional vacuolar H(+)-ATPase. Mol Cell Biol. 1995;15:4103-14 pubmed
    ..Mutations in the genes for yeast protein kinase C (pkc1) and a MAP kinase (mpk1/slt2) disrupt a Ca(2+)-dependent signaling pathway required to maintain a ..
  34. Romeo M, Angus Hill M, Sobering A, Kamada Y, Cairns B, Levin D. HTL1 encodes a novel factor that interacts with the RSC chromatin remodeling complex in Saccharomyces cerevisiae. Mol Cell Biol. 2002;22:8165-74 pubmed
    ..a genetic screen for mutants that displayed additive growth defects with a conditional mutation in the protein kinase C gene (PKC1), which has been suggested through genetic connections to interact functionally with RSC...
  35. Vannier D, Damay P, Shore D. A role for Sds3p, a component of the Rpd3p/Sin3p deacetylase complex, in maintaining cellular integrity in Saccharomyces cerevisiae. Mol Genet Genomics. 2001;265:560-8 pubmed
    ..Significantly, growth in the presence of 1 M sorbitol or overexpression of PKC1 also partially suppresses the lethal phenotype of the sds3 swi6 strain...
  36. Martin Yken H, Dagkessamanskaia A, de Groot P, Ram A, Klis F, Francois J. Saccharomyces cerevisiae YCRO17c/CWH43 encodes a putative sensor/transporter protein upstream of the BCK2 branch of the PKC1-dependent cell wall integrity pathway. Yeast. 2001;18:827-40 pubmed
    ..isolated for its Calcofluor white hypersensitivity, displays several cell wall defects similar to mutants in the PKC1-MPK1 pathway, including a growth defect and increased release of beta-1,6-glucan and beta-glucosylated proteins ..
  37. Hosotani T, Koyama H, Uchino M, Miyakawa T, Tsuchiya E. PKC1, a protein kinase C homologue of Saccharomyces cerevisiae, participates in microtubule function through the yeast EB1 homologue, BIM1. Genes Cells. 2001;6:775-88 pubmed
    ..Amongst the suppressors we cloned PKC1/STT1 and BIM1 that encoded a homologue of mammalian protein kinase C and a conserved microtubule binding protein homologous to human EB1, respectively...
  38. García Rodriguez L, Duran A, Roncero C. Calcofluor antifungal action depends on chitin and a functional high-osmolarity glycerol response (HOG) pathway: evidence for a physiological role of the Saccharomyces cerevisiae HOG pathway under noninducing conditions. J Bacteriol. 2000;182:2428-37 pubmed
  39. Bouquin N, Johnson A, Morgan B, Johnston L. Association of the cell cycle transcription factor Mbp1 with the Skn7 response regulator in budding yeast. Mol Biol Cell. 1999;10:3389-400 pubmed
    ..Thus, Skn7 and Mbp1 seem to form a transcription factor independent of MBF. Genetic data suggest that this new transcription factor could be involved in the bud-emergence process. ..
  40. Kaeberlein M, Guarente L. Saccharomyces cerevisiae MPT5 and SSD1 function in parallel pathways to promote cell wall integrity. Genetics. 2002;160:83-95 pubmed
    ..This work also provides evidence that post-transcriptional regulation is likely to be important both for maintaining cell integrity and for promoting longevity. ..
  41. Mitjana F, Petkova M, Pujol Carrion N, De La Torre Ruiz M. Pkc1 and actin polymerisation activities play a role in ribosomal gene repression associated with secretion impairment caused by oxidative stress. FEMS Yeast Res. 2011;11:656-9 pubmed publisher
    ..In this study, we show that the Pkc1 protein mediates oxidative signalling by helping to downregulate ribosomal gene expression when cells are exposed ..
  42. Logan M, Jones L, Eitzen G. Cdc42p and Rho1p are sequentially activated and mechanistically linked to vacuole membrane fusion. Biochem Biophys Res Commun. 2010;394:64-9 pubmed publisher
    ..These results define unique activation mechanisms for Cdc42p and Rho1p, which may be linked to the vacuole membrane fusion mechanism. ..
  43. Harrison J, Zyla T, Bardes E, Lew D. Stress-specific activation mechanisms for the "cell integrity" MAPK pathway. J Biol Chem. 2004;279:2616-22 pubmed
    ..These findings suggest that stresses provide "lateral" inputs into this regulatory pathway, rather than operating in a linear "top-down" manner. ..
  44. Fadri M, Daquinag A, Wang S, Xue T, Kunz J. The pleckstrin homology domain proteins Slm1 and Slm2 are required for actin cytoskeleton organization in yeast and bind phosphatidylinositol-4,5-bisphosphate and TORC2. Mol Biol Cell. 2005;16:1883-900 pubmed
  45. Merlini L, Bolognesi A, Juanes M, Vandermoere F, Courtellemont T, Pascolutti R, et al. Rho1- and Pkc1-dependent phosphorylation of the F-BAR protein Syp1 contributes to septin ring assembly. Mol Biol Cell. 2015;26:3245-62 pubmed publisher
    ..a genetic screen, we uncovered an unanticipated link between the conserved Rho1 GTPase and its effector protein kinase C (Pkc1) with septin ring stability in yeast...
  46. Philips J, Herskowitz I. Identification of Kel1p, a kelch domain-containing protein involved in cell fusion and morphology in Saccharomyces cerevisiae. J Cell Biol. 1998;143:375-89 pubmed
    We showed previously that protein kinase C, which is required to maintain cell integrity, negatively regulates cell fusion (Philips, J., and I. Herskowitz. 1997. J. Cell Biol. 138:961-974)...
  47. Pujol N, Bonet C, Vilella F, Petkova M, Mozo Villarías A, De La Torre Ruiz M. Two proteins from Saccharomyces cerevisiae: Pfy1 and Pkc1, play a dual role in activating actin polymerization and in increasing cell viability in the adaptive response to oxidative stress. FEMS Yeast Res. 2009;9:1196-207 pubmed publisher
    In this work, we show that the proteins Pkc1 and Pfy1 play a role in the repolarization of the actin cytoskeleton and in cell survival in response to oxidative stress...
  48. Lottersberger F, Panza A, Lucchini G, Piatti S, Longhese M. The Saccharomyces cerevisiae 14-3-3 proteins are required for the G1/S transition, actin cytoskeleton organization and cell wall integrity. Genetics. 2006;173:661-75 pubmed
    ..Both the protein kinase C1 (Pkc1) and its upstream regulators Wsc2 and Mid2 were found to act as high dosage suppressors of bmh mutants' temperature ..
  49. O Donnell A, Stevens J, Kepkay R, Barnes C, Johnston G, Singer R. New mutant versions of yeast FACT subunit Spt16 affect cell integrity. Mol Genet Genomics. 2009;282:487-502 pubmed publisher
    ..The overexpression of components of protein kinase C (Pkc1) signaling diminishes this spt16 ssd1-d temperature sensitivity, whereas gene deletions eliminating ..
  50. Roemer T, Paravicini G, Payton M, Bussey H. Characterization of the yeast (1-->6)-beta-glucan biosynthetic components, Kre6p and Skn1p, and genetic interactions between the PKC1 pathway and extracellular matrix assembly. J Cell Biol. 1994;127:567-79 pubmed
    ..Deletion of the yeast protein kinase C homolog, PKC1, leads to a lysis defect (Levin, D. E., and E. Bartlett-Heubusch. 1992. Mutants in the S...
  51. Stirling D, Stark M. Mutations in SPC110, encoding the yeast spindle pole body calmodulin-binding protein, cause defects in cell integrity as well as spindle formation. Biochim Biophys Acta. 2000;1499:85-100 pubmed
    ..Our findings demonstrate a role for calcium in the Spc110p-calmodulin interaction in vivo and have important general implications for the interpretation of genetic interactions involving cell integrity genes. ..
  52. Stewart M, Krause S, McGhie J, Gray J. Mpt5p, a stress tolerance- and lifespan-promoting PUF protein in Saccharomyces cerevisiae, acts upstream of the cell wall integrity pathway. Eukaryot Cell. 2007;6:262-70 pubmed
    ..We conclude that Mpt5p is required for normal replicative and chronological life spans and that the CWI pathway is a key and direct downstream target of this PUF protein. ..
  53. Mensonides F, Brul S, Klis F, Hellingwerf K, Teixeira De Mattos M. Activation of the protein kinase C1 pathway upon continuous heat stress in Saccharomyces cerevisiae is triggered by an intracellular increase in osmolarity due to trehalose accumulation. Appl Environ Microbiol. 2005;71:4531-8 pubmed
    ..A detailed analysis was carried out of the accumulation of trehalose and the activation of the protein kinase C1 (PKC1) (cell integrity) pathway in both glucose- and ethanol-grown cells upon temperature upshifts within this narrow ..
  54. Loewith R, Jacinto E, Wullschleger S, Lorberg A, Crespo J, Bonenfant D, et al. Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol Cell. 2002;10:457-68 pubmed
    ..Thus, the distinct TOR complexes account for the diversity, specificity, and selective rapamycin inhibition of TOR signaling. TORC1 and possibly TORC2 are conserved from yeast to man. ..
  55. Juanes M, Queralt E, Bañó M, Igual J. Rot1 plays an antagonistic role to Clb2 in actin cytoskeleton dynamics throughout the cell cycle. J Cell Sci. 2007;120:2390-401 pubmed
    ..Several genetic interactions suggest a link between Rot1 and the ubiquitin-proteasome system and we show that the Clb2 cyclin is not properly degraded in rot1 cells. ..
  56. Yashar B, Irie K, Printen J, Stevenson B, Sprague G, Matsumoto K, et al. Yeast MEK-dependent signal transduction: response thresholds and parameters affecting fidelity. Mol Cell Biol. 1995;15:6545-53 pubmed
    ..This behavior suggests that Ste5p, which has been proposed to be a tether for the kinases in the mating pathway, contributes to Ste7p specificity. ..
  57. Lin A, Patel S, Latterich M. Regulation of organelle membrane fusion by Pkc1p. Traffic. 2001;2:698-704 pubmed
    ..We provide first evidence that endoplasmic reticulum membrane fusion in yeast is regulated by the action of protein kinase C. Specifically, Pkc1p kinase activity is needed to protect the fusion machinery from ubiquitin-mediated ..
  58. Luesch H, Wu T, Ren P, Gray N, Schultz P, Supek F. A genome-wide overexpression screen in yeast for small-molecule target identification. Chem Biol. 2005;12:55-63 pubmed
    ..1), an inhibitor identified from a kinase-directed library, was associated with the overexpression of Pkc1 and a subset of downstream kinases...
  59. Darieva Z, Han N, Warwood S, Doris K, Morgan B, Sharrocks A. Protein kinase C regulates late cell cycle-dependent gene expression. Mol Cell Biol. 2012;32:4651-61 pubmed publisher
    ..This study therefore identifies an important role for Pkc1p in controlling the correct temporal expression of genes in the cell cycle. ..
  60. Chai B, Hsu J, Du J, Laurent B. Yeast RSC function is required for organization of the cellular cytoskeleton via an alternative PKC1 pathway. Genetics. 2002;161:575-84 pubmed
    ..Slg1p and Mid2p, two type I transmembrane stress sensors of cell wall integrity that function upstream of protein kinase C (Pkc1p), were identified as multicopy suppressors of sth1-3ts cells...
  61. Nomura W, Inoue Y. Methylglyoxal activates the target of rapamycin complex 2-protein kinase C signaling pathway in Saccharomyces cerevisiae. Mol Cell Biol. 2015;35:1269-80 pubmed publisher
    Methylglyoxal is a typical 2-oxoaldehyde derived from glycolysis. We show here that methylglyoxal activates the Pkc1-Mpk1 mitogen-activated protein (MAP) kinase cascade in a target of rapamycin complex 2 (TORC2)-dependent manner in the ..
  62. Nomura W, Maeta K, Inoue Y. Phosphatidylinositol 3,5-bisphosphate is involved in methylglyoxal-induced activation of the Mpk1 mitogen-activated protein kinase cascade in Saccharomyces cerevisiae. J Biol Chem. 2017;292:15039-15048 pubmed publisher
    ..MG activated the Pkc1-Mpk1 MAPK cascade in which a small GTPase Rho1 plays a crucial role, and the MG-induced phosphorylation of Mpk1 was ..
  63. Shen Z, Li Y, Gasparski A, Abeliovich H, Greenberg M. Cardiolipin Regulates Mitophagy through the Protein Kinase C Pathway. J Biol Chem. 2017;292:2916-2923 pubmed publisher
    ..MAPK Slt2p was defective in crd1Δ cells, and up-regulation of the PKC pathway by expression of the PKC1R398P gene, which encodes constitutively activated Pkc1p, rescued crd1Δ growth and ..
  64. Yoshida S, Ohya Y, Hirose R, Nakano A, Anraku Y. STT10, a novel class-D VPS yeast gene required for osmotic integrity related to the PKC1/STT1 protein kinase pathway. Gene. 1995;160:117-22 pubmed
    ..ST sensitivity of the stt10 mutant was suppressed by overexpression of PKC1/STT1, showing the genetic interactions of STT10 with the PKC1/STT1 pathway...
  65. Choi M, Park T, Carman G. Phosphorylation of Saccharomyces cerevisiae CTP synthetase at Ser424 by protein kinases A and C regulates phosphatidylcholine synthesis by the CDP-choline pathway. J Biol Chem. 2003;278:23610-6 pubmed
    ..Using a purified S424A mutant CTP synthetase enzyme, we examined the effect of Ser424 phosphorylation on protein kinase C phosphorylation...
  66. Paravicini G, Friedli L. Protein-protein interactions in the yeast PKC1 pathway: Pkc1p interacts with a component of the MAP kinase cascade. Mol Gen Genet. 1996;251:682-91 pubmed
    ..for proteins that interact in vivo with the Saccharomyces cerevisiae Pkc1 protein, a homolog of mammalian protein kinase C. Four positive clones were isolated that encoded portions of the protein kinase Mkk1, which acts downstream ..
  67. Soriano Carot M, Quilis I, Bañó M, Igual J. Protein kinase C controls activation of the DNA integrity checkpoint. Nucleic Acids Res. 2014;42:7084-95 pubmed publisher
    The protein kinase C (PKC) superfamily plays key regulatory roles in numerous cellular processes. Saccharomyces cerevisiae contains a single PKC, Pkc1, whose main function is cell wall integrity maintenance...
  68. Yano K, Uesono Y, Yoshida S, Kikuchi A, Kashiwazaki J, Mabuchi I, et al. Mih1/Cdc25 is negatively regulated by Pkc1 in Saccharomyces cerevisiae. Genes Cells. 2013;18:425-41 pubmed publisher
    ..The band pattern of Mih1 by SDS-PAGE was similar to that of Mih1(4A) only after inactivation of Pkc1 in a pkc1(ts) mutant...
  69. Tohe A, Oguchi T. Isolation and characterization of the yeast las21 mutants, which are sensitive to a local anestheticum, tetracaine. Genes Genet Syst. 1998;73:365-75 pubmed
    ..temperature sensitivity of the las21 mutants was partially suppressed by introduction of PKC1, encoding protein kinase C, on a high copy vector. We found some genetic interactions between LAS21 and Ras/cAMP cascade genes...
  70. Park H, Lennarz W. Evidence for interaction of yeast protein kinase C with several subunits of oligosaccharyl transferase. Glycobiology. 2000;10:737-44 pubmed
    ..Studies on potential protein-protein interactions in OT using a two-hybrid library screen revealed that protein kinase C (Pkc1p) interacted with the lumenal domains of several OT subunits...
  71. Barker C, Mouchel N, Jenkins J. The identification and characterisation of a functional interaction between arginyl-tRNA-protein transferase and topoisomerase II. Biochem Biophys Res Commun. 2006;342:596-604 pubmed
    ..Ate1 is a component of the N-end rule protein degradation pathway which targets proteins for degradation. We also propose a previously unidentified role for Ate1 in modulating the level of topoisomerase II through the cell cycle. ..
  72. Basmaji F, Martin Yken H, Durand F, Dagkessamanskaia A, Pichereaux C, Rossignol M, et al. The 'interactome' of the Knr4/Smi1, a protein implicated in coordinating cell wall synthesis with bud emergence in Saccharomyces cerevisiae. Mol Genet Genomics. 2006;275:217-30 pubmed
    The integrity of the Saccharomyces cerevisiae cell wall requires a functional Pkc1-Slt2 MAP kinase pathway that contributes to transient growth arrest, enabling coordination of cell division with cell wall remodelling...
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    ..In this work we examined the hypothesis that choline kinase is also phosphorylated by protein kinase C. Using choline kinase as a substrate, protein kinase C activity was dose- and time-dependent and dependent on ..
  74. Andrews P, Stark M. Type 1 protein phosphatase is required for maintenance of cell wall integrity, morphogenesis and cell cycle progression in Saccharomyces cerevisiae. J Cell Sci. 2000;113 ( Pt 3):507-20 pubmed
    ..Pkc1p, the yeast protein kinase C homolog which is thought to regulate the Mpk1p MAP kinase pathway involved in cell wall remodelling and ..