Gene Symbol: PIS1
Description: CDP-diacylglycerol--inositol 3-phosphatidyltransferase
Alias: CDP-diacylglycerol--inositol 3-phosphatidyltransferase
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Kuchler K, Daum G, Paltauf F. Subcellular and submitochondrial localization of phospholipid-synthesizing enzymes in Saccharomyces cerevisiae. J Bacteriol. 1986;165:901-10 pubmed
    ..Data concerning susceptibility to various inhibitors, heat sensitivity, and the pH optima indicate that there is a close similarity of the mitochondrial and microsomal enzymes that catalyze the same reaction. ..
  2. Nikawa J, Yamashita S. Molecular cloning of the gene encoding CDPdiacylglycerol-inositol 3-phosphatidyl transferase in Saccharomyces cerevisiae. Eur J Biochem. 1984;143:251-6 pubmed
    ..Thus, the availability of myo-inositol is a critical regulatory factor in yeast phosphatidylinositol synthesis. ..
  3. Carman G, Matas J. Solubilization of microsomal-associated phosphatidylserine synthase and phosphatidylinositol synthase from Saccharomyces cerevisiae. Can J Microbiol. 1981;27:1140-9 pubmed
    ..1 mM, respectively. Thioreactive agents inhibited both enzymatic activities. Both solubilized enzymatic activities were thermally inactivated at temperatures above 30 degrees C. ..
  4. Gardocki M, Bakewell M, Kamath D, Robinson K, Borovicka K, Lopes J. Genomic analysis of PIS1 gene expression. Eukaryot Cell. 2005;4:604-14 pubmed
    The Saccharomyces cerevisiae PIS1 gene is essential and required for the final step in the de novo synthesis of phosphatidylinositol...
  5. Han S, Han G, Iwanyshyn W, Carman G. Regulation of the PIS1-encoded phosphatidylinositol synthase in Saccharomyces cerevisiae by zinc. J Biol Chem. 2005;280:29017-24 pubmed
    ..M. (2004) J. Biol. Chem. 279, 21976-21983). We examined the effects of zinc depletion on the regulation of the PIS1-encoded phosphatidylinositol synthase, the enzyme that catalyzes the formation of phosphatidylinositol from CDP-..
  6. Iverson S, Sonnemann K, Reddick A, McDonough V. Expression of the Saccharomyces cerevisiae PIS1 gene is modulated by multiple ATGs in the promoter. Biochem Biophys Res Commun. 2006;350:91-6 pubmed
    The PIS1 gene encodes a key branchpoint phospholipid biosynthetic enzyme, phosphatidylinositol synthase...
  7. Jani N, Lopes J. Transcription regulation of the Saccharomyces cerevisiae PIS1 gene by inositol and the pleiotropic regulator, Ume6p. Mol Microbiol. 2008;70:1529-39 pubmed publisher
    ..This regulation requires the Ino2p and Ino4p activators and the Opi1p repressor. The PIS1 structural gene is required for the synthesis of the essential lipid phosphatidylinositol...
  8. Bochud A, Conzelmann A. The active site of yeast phosphatidylinositol synthase Pis1 is facing the cytosol. Biochim Biophys Acta. 2015;1851:629-40 pubmed publisher
    ..Bioinformatic tools predict the CAPT motif of phosphatidylinositol synthase Pis1 as either ER luminal or cytosolic...
  9. Yamagami K, Yamamoto T, Sakai S, Mioka T, Sano T, Igarashi Y, et al. Inositol depletion restores vesicle transport in yeast phospholipid flippase mutants. PLoS ONE. 2015;10:e0120108 pubmed publisher
    ..These results suggest that inositol depletion changes the lipid composition of the endosomal/TGN membranes, which results in vesicle formation from these membranes in the absence of flippases. ..

More Information


  1. Fischl A, Homann M, Poole M, Carman G. Phosphatidylinositol synthase from Saccharomyces cerevisiae. Reconstitution, characterization, and regulation of activity. J Biol Chem. 1986;261:3178-83 pubmed
    ..Phosphatidylserine stimulated reconstituted activity, suggesting that the local phospholipid environment may regulate phosphatidylinositol synthase activity. ..
  2. Nikawa J, Kodaki T, Yamashita S. Primary structure and disruption of the phosphatidylinositol synthase gene of Saccharomyces cerevisiae. J Biol Chem. 1987;262:4876-81 pubmed
    ..The nonviable spores were in an arrested state with a characteristic terminal phenotype, suggesting that the function of the PIS gene is essential for progression of the yeast cell cycle. ..
  3. Leber A, Hrastnik C, Daum G. Phospholipid-synthesizing enzymes in Golgi membranes of the yeast, Saccharomyces cerevisiae. FEBS Lett. 1995;377:271-4 pubmed
    ..Two phospholipid-synthesizing enzymes, namely phosphatidylinositol synthase and sn-1,2-diacylglycerol cholinephosphotransferase, are detected in the Golgi at a specific activity which exceeds that of the endoplasmic reticulum. ..
  4. Kaliszewski P, Ferreira T, Gajewska B, Szkopinska A, Berges T, ZoƂadek T. Enhanced levels of Pis1p (phosphatidylinositol synthase) improve the growth of Saccharomyces cerevisiae cells deficient in Rsp5 ubiquitin ligase. Biochem J. 2006;395:173-81 pubmed
    ..The PIS1 (phosphatidylinositol synthase gene) encoding the enzyme Pis1p which catalyses the synthesis of ..
  5. Tamura Y, Harada Y, Nishikawa S, Yamano K, Kamiya M, Shiota T, et al. Tam41 is a CDP-diacylglycerol synthase required for cardiolipin biosynthesis in mitochondria. Cell Metab. 2013;17:709-18 pubmed publisher
    ..The present findings unveil the missing step of the cardiolipin synthesis pathway in mitochondria as well as the flexibile regulation of phospholipid biosynthesis to respond to compromised CDP-DAG synthesis in mitochondria. ..