Gene Symbol: PIF1
Description: DNA helicase PIF1
Alias: TST1, DNA helicase PIF1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Smith S, Gupta A, Kolodner R, Myung K. Suppression of gross chromosomal rearrangements by the multiple functions of the Mre11-Rad50-Xrs2 complex in Saccharomyces cerevisiae. DNA Repair (Amst). 2005;4:606-17 pubmed
    ..However, the non-homologous end joining function of MRX complex does not appear to participate in the suppression of GCRs. ..
  2. Rossi M, Pike J, Wang W, Burgers P, Campbell J, Bambara R. Pif1 helicase directs eukaryotic Okazaki fragments toward the two-nuclease cleavage pathway for primer removal. J Biol Chem. 2008;283:27483-93 pubmed publisher
    ..Therefore, we asked whether other relevant proteins promote long flap cleavage via the Dna2 pathway. The Pif1 helicase, implicated in Okazaki maturation from genetic studies, improved flap displacement and increased RPA ..
  3. Ribeyre C, Lopes J, Boulé J, Piazza A, Guédin A, Zakian V, et al. The yeast Pif1 helicase prevents genomic instability caused by G-quadruplex-forming CEB1 sequences in vivo. PLoS Genet. 2009;5:e1000475 pubmed publisher
    In budding yeast, the Pif1 DNA helicase is involved in the maintenance of both nuclear and mitochondrial genomes, but its role in these processes is still poorly understood...
  4. Downey M, Houlsworth R, Maringele L, Rollie A, Brehme M, Galicia S, et al. A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator. Cell. 2006;124:1155-68 pubmed
    ..Our results therefore indicate that the KEOPS complex promotes both telomere uncapping and telomere elongation. ..
  5. Schulz V, Zakian V. The saccharomyces PIF1 DNA helicase inhibits telomere elongation and de novo telomere formation. Cell. 1994;76:145-55 pubmed
    A screen to detect yeast mutants that frequently lost expression of subtelomeric genes identified two mutations in PIF1, a gene known to encode a 5' to 3' DNA helicase...
  6. Galletto R, Tomko E. Translocation of Saccharomyces cerevisiae Pif1 helicase monomers on single-stranded DNA. Nucleic Acids Res. 2013;41:4613-27 pubmed publisher
    In Saccharomyces cerevisiae Pif1 participates in a wide variety of DNA metabolic pathways both in the nucleus and in mitochondria...
  7. Dewar J, Lydall D. Pif1- and Exo1-dependent nucleases coordinate checkpoint activation following telomere uncapping. EMBO J. 2010;29:4020-34 pubmed publisher
    ..budding yeast, we show that the telomere capping protein Cdc13 protects telomeres from the activity of the helicase Pif1 and the exonuclease Exo1...
  8. Hwang J, Smith S, Myung K. The Rad1-Rad10 complex promotes the production of gross chromosomal rearrangements from spontaneous DNA damage in Saccharomyces cerevisiae. Genetics. 2005;169:1927-37 pubmed
    ..Results presented here suggest that Rad1-Rad10 functions at different stages of GCR formation and that there is an alternative pathway for the GCR formation that is independent of Rad1-Rad10. ..
  9. Myung K, Chen C, Kolodner R. Multiple pathways cooperate in the suppression of genome instability in Saccharomyces cerevisiae. Nature. 2001;411:1073-6 pubmed
    ..Mutations that inactivate these pathways cause high rates of GCRs and show synergistic interactions, indicating that the pathways that suppress GCRs all compete for the same DNA substrates. ..

More Information


  1. Putnam C, Hayes T, Kolodner R. Post-replication repair suppresses duplication-mediated genome instability. PLoS Genet. 2010;6:e1000933 pubmed publisher
    ..Our analysis is consistent with models in which PRR prevents replication damage from becoming double strand breaks (DSBs) and/or regulates the activity of HR on DSBs. ..
  2. Cheng X, Dunaway S, Ivessa A. The role of Pif1p, a DNA helicase in Saccharomyces cerevisiae, in maintaining mitochondrial DNA. Mitochondrion. 2007;7:211-22 pubmed
    ..In Saccharomyces cerevisiae, the DNA helicase Pif1p is localized to the nucleus and mitochondria. We show that pif1 mutant cells are sensitive to ethidium bromide-induced damage and this mtDNA is prone to fragmentation...
  3. Wagner M, Price G, Rothstein R. The absence of Top3 reveals an interaction between the Sgs1 and Pif1 DNA helicases in Saccharomyces cerevisiae. Genetics. 2006;174:555-73 pubmed
    ..We searched for gene dosage suppressors of top3 and identified Pif1, a DNA helicase that acts with polarity opposite to that of Sgs1...
  4. Lahaye A, Leterme S, Foury F. PIF1 DNA helicase from Saccharomyces cerevisiae. Biochemical characterization of the enzyme. J Biol Chem. 1993;268:26155-61 pubmed
    Overexpressed PIF1 DNA helicase was purified from mitochondria to near homogeneity. Its ATPase and unwinding properties were characterized. The enzyme specifically utilizes ATP (or dATP) and MgCl2 (and to a lesser extent MnCl2)...
  5. Wilson M, Kwon Y, Xu Y, Chung W, Chi P, Niu H, et al. Pif1 helicase and Pol? promote recombination-coupled DNA synthesis via bubble migration. Nature. 2013;502:393-6 pubmed publisher
    ..A good candidate DNA helicase is Pif1, an evolutionarily conserved helicase in Saccharomyces cerevisiae important for break-induced replication (BIR) as ..
  6. Cheng X, Qin Y, Ivessa A. Loss of mitochondrial DNA under genotoxic stress conditions in the absence of the yeast DNA helicase Pif1p occurs independently of the DNA helicase Rrm3p. Mol Genet Genomics. 2009;281:635-45 pubmed publisher
    ..We demonstrate that the inefficient mtDNA replication process of mutant yeast cells lacking the PIF1 DNA helicase is partly rescued in the absence of the DNA helicase RRM3...
  7. Budd M, Reis C, Smith S, Myung K, Campbell J. Evidence suggesting that Pif1 helicase functions in DNA replication with the Dna2 helicase/nuclease and DNA polymerase delta. Mol Cell Biol. 2006;26:2490-500 pubmed
    ..In this work, we present evidence that Saccharomyces cerevisiae Pif1 helicase plays a wider role in DNA replication than previously appreciated and that it likely functions in ..
  8. Taylor S, Zhang H, Eaton J, Rodeheffer M, Lebedeva M, O Rourke T, et al. The conserved Mec1/Rad53 nuclear checkpoint pathway regulates mitochondrial DNA copy number in Saccharomyces cerevisiae. Mol Biol Cell. 2005;16:3010-8 pubmed
    ..In addition, deletion of RRM3 or SML1 fully rescued the approximately 50% depletion of mtDNA observed in a pif1 null strain...
  9. Stith C, Sterling J, Resnick M, Gordenin D, Burgers P. Flexibility of eukaryotic Okazaki fragment maturation through regulated strand displacement synthesis. J Biol Chem. 2008;283:34129-40 pubmed publisher
    ..Our genetic studies show that deletion of the POL32 (third subunit of Pol delta) or PIF1 helicase genes can suppress lethality or growth defects of rad27Delta pol3-D520V mutants (defective for FEN1(RAD27) ..
  10. Pike J, Burgers P, Campbell J, Bambara R. Pif1 helicase lengthens some Okazaki fragment flaps necessitating Dna2 nuclease/helicase action in the two-nuclease processing pathway. J Biol Chem. 2009;284:25170-80 pubmed publisher
    ..Recent reconstitution results indicated that Pif1 helicase, a known component of fragment processing, accelerated flap displacement, allowing the inhibitory action ..
  11. Vega L, Phillips J, Thornton B, Benanti J, Onigbanjo M, Toczyski D, et al. Sensitivity of yeast strains with long G-tails to levels of telomere-bound telomerase. PLoS Genet. 2007;3:e105 pubmed
    ..mutants lacking this interaction, telomeres were longer in the absence of Pif1p than in the presence of wild-type PIF1, indicating that endogenous Pif1p inhibits the active S/G2 form of telomerase...
  12. Banerjee S, Smith S, Myung K. Suppression of gross chromosomal rearrangements by yKu70-yKu80 heterodimer through DNA damage checkpoints. Proc Natl Acad Sci U S A. 2006;103:1816-21 pubmed
    ..We also demonstrate that the inactivation of telomerase inhibitor, Pif1 along with Ku overexpression or the overexpression of Pif1 in either yku70 or yku80 strains arrested the cell cycle ..
  13. Chen H, Lisby M, Symington L. RPA coordinates DNA end resection and prevents formation of DNA hairpins. Mol Cell. 2013;50:589-600 pubmed publisher
    ..Thus, RPA is required to generate ssDNA, and also to protect ssDNA from degradation and inappropriate annealing that could lead to genome rearrangements. ..
  14. Zhang W, Durocher D. De novo telomere formation is suppressed by the Mec1-dependent inhibition of Cdc13 accumulation at DNA breaks. Genes Dev. 2010;24:502-15 pubmed publisher
    ..These studies therefore identify a mechanism by which the ATR family of kinases enforces genome integrity, and a process that underscores the contribution of Cdc13 to the fate of DNA ends. ..
  15. Lahaye A, Stahl H, Thines Sempoux D, Foury F. PIF1: a DNA helicase in yeast mitochondria. EMBO J. 1991;10:997-1007 pubmed
    The PIF1 gene is involved in repair and recombination of mitochondrial DNA (mtDNA)...
  16. Barranco Medina S, Galletto R. DNA binding induces dimerization of Saccharomyces cerevisiae Pif1. Biochemistry. 2010;49:8445-54 pubmed publisher
    In Saccharomyces cerevisiae, Pif1 is involved in a wide range of DNA transactions. It operates both in mitochondria and in the nucleus, where it has telomeric and non-telomeric functions...
  17. Paeschke K, Capra J, Zakian V. DNA replication through G-quadruplex motifs is promoted by the Saccharomyces cerevisiae Pif1 DNA helicase. Cell. 2011;145:678-91 pubmed publisher
    ..was used to determine the in vivo binding sites of the multifunctional Saccharomyces cerevisiae Pif1 DNA helicase, a potent unwinder of G4 structures in vitro...
  18. O Rourke T, Doudican N, Zhang H, Eaton J, Doetsch P, Shadel G. Differential involvement of the related DNA helicases Pif1p and Rrm3p in mtDNA point mutagenesis and stability. Gene. 2005;354:86-92 pubmed
    ..Altogether, our results define a novel role for Rrm3p in mitochondrial function and indicate that Pif1p and Rrm3p influence a common process (or processes) involved in mtDNA replication, repair, or stability. ..
  19. Boulé J, Vega L, Zakian V. The yeast Pif1p helicase removes telomerase from telomeric DNA. Nature. 2005;438:57-61 pubmed
    ..We propose that Pif1p helicase activity limits telomerase action both in vivo and in vitro by displacing active telomerase from DNA ends. ..
  20. Eugster A, Lanzuolo C, Bonneton M, Luciano P, Pollice A, Pulitzer J, et al. The finger subdomain of yeast telomerase cooperates with Pif1p to limit telomere elongation. Nat Struct Mol Biol. 2006;13:734-9 pubmed
    ..We propose that, in addition to its catalytic role, the finger subdomain of Est2p facilitates the action of Pif1p at telomeres. ..
  21. Cheng X, Ivessa A. Association of the yeast DNA helicase Pif1p with mitochondrial membranes and mitochondrial DNA. Eur J Cell Biol. 2010;89:742-7 pubmed publisher
    ..We also found that in the absence of mtDNA the total amount of cellular Pif1p is highly reduced. ..
  22. Ramanagoudr Bhojappa R, Chib S, Byrd A, Aarattuthodiyil S, Pandey M, Patel S, et al. Yeast Pif1 helicase exhibits a one-base-pair stepping mechanism for unwinding duplex DNA. J Biol Chem. 2013;288:16185-95 pubmed publisher
    ..Saccharomyces cerevisiae Pif1 helicase was characterized using presteady state kinetics to determine rates of DNA unwinding, displacement of ..
  23. Paeschke K, Bochman M, Garcia P, Cejka P, Friedman K, Kowalczykowski S, et al. Pif1 family helicases suppress genome instability at G-quadruplex motifs. Nature. 2013;497:458-62 pubmed publisher
    The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans...
  24. Motegi A, Kuntz K, Majeed A, Smith S, Myung K. Regulation of gross chromosomal rearrangements by ubiquitin and SUMO ligases in Saccharomyces cerevisiae. Mol Cell Biol. 2006;26:1424-33 pubmed
    ..We propose a mechanism for how defects in these proteins could lead to diverse outcomes (proper repair or GCR formation) through different regulation of DNA repair machinery. ..
  25. Zhou J, Monson E, Teng S, Schulz V, Zakian V. Pif1p helicase, a catalytic inhibitor of telomerase in yeast. Science. 2000;289:771-4 pubmed
    Mutations in the yeast Saccharomyces cerevisiae PIF1 gene, which encodes a 5'-to-3' DNA helicase, cause telomere lengthening and a large increase in the formation rate of new telomeres...
  26. Pike J, Henry R, Burgers P, Campbell J, Bambara R. An alternative pathway for Okazaki fragment processing: resolution of fold-back flaps by Pif1 helicase. J Biol Chem. 2010;285:41712-23 pubmed publisher
    ..A small fraction escapes immediate FEN1 cleavage and is further lengthened by Pif1 helicase. Long flaps are bound by replication protein A (RPA), which inhibits FEN1...
  27. Boulé J, Zakian V. The yeast Pif1p DNA helicase preferentially unwinds RNA DNA substrates. Nucleic Acids Res. 2007;35:5809-18 pubmed
    Pif1p is the prototypical member of the PIF1 family of DNA helicases, a subfamily of SFI helicases conserved from yeast to humans...
  28. Saini N, Ramakrishnan S, Elango R, Ayyar S, Zhang Y, Deem A, et al. Migrating bubble during break-induced replication drives conservative DNA synthesis. Nature. 2013;502:389-92 pubmed publisher
    ..We provide evidence that this atypical mode of DNA replication, dependent on Pif1 helicase, is responsible for the marked increase in BIR-associated mutations...
  29. Smith S, Banerjee S, Rilo R, Myung K. Dynamic regulation of single-stranded telomeres in Saccharomyces cerevisiae. Genetics. 2008;178:693-701 pubmed publisher
    ..Mutating the helicase domain of the telomerase inhibitor Pif1 resulted in the inactivation of cell cycle checkpoints and the subsequent rescue of temperature sensitivity of the ..
  30. Bonetti D, Villa M, Gobbini E, Cassani C, Tedeschi G, Longhese M. Escape of Sgs1 from Rad9 inhibition reduces the requirement for Sae2 and functional MRX in DNA end resection. EMBO Rep. 2015;16:351-61 pubmed publisher
    ..These results provide new insights into how early and long-range resection is coordinated. ..
  31. Hang L, Liu X, Cheung I, Yang Y, Zhao X. SUMOylation regulates telomere length homeostasis by targeting Cdc13. Nat Struct Mol Biol. 2011;18:920-6 pubmed publisher
  32. Guirola M, Barreto L, Pagani A, Romagosa M, Casamayor A, Atrian S, et al. Lack of DNA helicase Pif1 disrupts zinc and iron homoeostasis in yeast. Biochem J. 2010;432:595-605 pubmed publisher
    The Saccharomyces cerevisiae gene PIF1 encodes a conserved eukaryotic DNA helicase required for both mitochondrial and nuclear DNA integrity. Our previous work revealed that a pif1? strain is tolerant to zinc overload...
  33. Ji H, Adkins C, Cartwright B, Friedman K. Yeast Est2p affects telomere length by influencing association of Rap1p with telomeric chromatin. Mol Cell Biol. 2008;28:2380-90 pubmed publisher
    ..Taken together, these results suggest that Est2p can directly or indirectly influence the binding of Rap1p to telomeric DNA, implicating telomerase in roles both upstream and downstream of Rap1p in telomere length homeostasis...
  34. Putnam C, Pallis K, Hayes T, Kolodner R. DNA repair pathway selection caused by defects in TEL1, SAE2, and de novo telomere addition generates specific chromosomal rearrangement signatures. PLoS Genet. 2014;10:e1004277 pubmed publisher
    ..Interestingly, the inverted duplications observed here resemble common GCRs in metastatic pancreatic cancer. ..
  35. Moriel Carretero M, Aguilera A. A postincision-deficient TFIIH causes replication fork breakage and uncovers alternative Rad51- or Pol32-mediated restart mechanisms. Mol Cell. 2010;37:690-701 pubmed publisher
    ..Our results define the genetic and molecular hallmarks of replication fork breakage and restart and bring insights to understand specific NER-related human syndromes. ..
  36. Hwang J, Smith S, Ceschia A, Torres Rosell J, Aragon L, Myung K. Smc5-Smc6 complex suppresses gross chromosomal rearrangements mediated by break-induced replications. DNA Repair (Amst). 2008;7:1426-36 pubmed publisher
  37. Chen Y, Yang C, Li R, Zeng R, Zhou J. Def1p is involved in telomere maintenance in budding yeast. J Biol Chem. 2005;280:24784-91 pubmed
    Saccharomyces Rrm3p, a member of Pif1 5'-3' DNA helicase subfamily, helps replication forks traverse protein-DNA complexes, including the telomere. Here we have identified an Rrm3p interaction protein known to be Def1p...
  38. Abraham K, Chan J, Salvi J, Ho B, Hall A, Vidya E, et al. Intersection of calorie restriction and magnesium in the suppression of genome-destabilizing RNA-DNA hybrids. Nucleic Acids Res. 2016;44:8870-8884 pubmed
    ..cell membrane Mg2+ transporters Alr1/2 act via Mg2+-sensitive R-loop suppressors Rnh1/201 and Pif1 to restore R-loop suppression, ribosomal DNA stability and cellular lifespan...
  39. Li J, Yu T, Chien I, Lu C, Lin J, Li H. Pif1 regulates telomere length by preferentially removing telomerase from long telomere ends. Nucleic Acids Res. 2014;42:8527-36 pubmed publisher
    ..b>Pif1, a 5'-to-3' helicase, has been suggested to regulate telomerase activity...
  40. Miller A, Daley J, Pham N, Niu H, Xue X, Ira G, et al. A novel role of the Dna2 translocase function in DNA break resection. Genes Dev. 2017;31:503-510 pubmed publisher
    ..Our study thus reveals a previously unrecognized role of the Dna2 translocase activity in DNA break end resection and in the imposition of the 5' strand specificity of end resection. ..
  41. Myung K, Kolodner R. Suppression of genome instability by redundant S-phase checkpoint pathways in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2002;99:4500-7 pubmed
    ..These data support the view that spontaneous genome rearrangements result from DNA replication errors and indicate that there is a high degree of redundancy among the checkpoints that act in S phase to suppress such genome instability. ..
  42. Salvi J, Chan J, Szafranski K, Liu T, Wu J, Olsen J, et al. Roles for Pbp1 and caloric restriction in genome and lifespan maintenance via suppression of RNA-DNA hybrids. Dev Cell. 2014;30:177-91 pubmed publisher
    ..through RNaseH overexpression, deletion of the G4DNA-stabilizing Stm1, or caloric restriction operating via RNaseH/Pif1 restores rDNA stability. Pbp1 also limits hybrids at non-rDNA G4DNA loci including telomeres...
  43. Van Dyck E, Jank B, Ragnini A, Schweyen R, Duyckaerts C, Sluse F, et al. Overexpression of a novel member of the mitochondrial carrier family rescues defects in both DNA and RNA metabolism in yeast mitochondria. Mol Gen Genet. 1995;246:426-36 pubmed
    The PIF1 and MRS2 gene products have previously been shown to be essential for mitochondrial DNA maintenance at elevated temperatures and mitochondrial group II intron splicing, respectively, in the yeast Saccharomyces cerevisiae...
  44. Meng F, Hu Y, Shen N, Tong X, Wang J, Ding J, et al. Sua5p a single-stranded telomeric DNA-binding protein facilitates telomere replication. EMBO J. 2009;28:1466-78 pubmed publisher
    ..Thus, Sua5p represents a novel ssTG DNA-binding protein and positively regulates the telomere length in vivo. ..
  45. Lian H, Robertson E, Hiraga S, Alvino G, Collingwood D, McCune H, et al. The effect of Ku on telomere replication time is mediated by telomere length but is independent of histone tail acetylation. Mol Biol Cell. 2011;22:1753-65 pubmed publisher
    ..regulates replication timing through its effect on telomere length, because deletion of the telomerase regulator Pif1 largely reverses the short telomere defect of a yku70 mutant and simultaneously rescues its replication timing ..
  46. Lu J, Liu Y. Deletion of Ogg1 DNA glycosylase results in telomere base damage and length alteration in yeast. EMBO J. 2010;29:398-409 pubmed publisher
  47. Singh S, Koc K, Stodola J, Galletto R. A Monomer of Pif1 Unwinds Double-Stranded DNA and It Is Regulated by the Nature of the Non-Translocating Strand at the 3'-End. J Mol Biol. 2016;428:1053-1067 pubmed publisher
    ..resonance energy transfer)-based helicase assays, in this work, we show that a monomer of Saccharomyces cerevisiae Pif1 can unwind dsDNA (double-stranded DNA)...
  48. Lopez C, Singh S, Hambarde S, Griffin W, Gao J, Chib S, et al. Yeast Sub1 and human PC4 are G-quadruplex binding proteins that suppress genome instability at co-transcriptionally formed G4 DNA. Nucleic Acids Res. 2017;45:5850-5862 pubmed publisher
    ..Finally, we demonstrate the physical and genetic interaction of Sub1 with the G4-resolving helicase Pif1, suggesting a possible mechanism by which Sub1 suppresses instability at G4 DNA.
  49. Myung K, Smith S, Kolodner R. Mitotic checkpoint function in the formation of gross chromosomal rearrangements in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2004;101:15980-5 pubmed
    ..These data suggest that functional mitotic checkpoints can play a role in the formation of genome rearrangements. ..
  50. Phillips J, Chan A, Paeschke K, Zakian V. The pif1 helicase, a negative regulator of telomerase, acts preferentially at long telomeres. PLoS Genet. 2015;11:e1005186 pubmed publisher
    Telomerase, the enzyme that maintains telomeres, preferentially lengthens short telomeres. The S. cerevisiae Pif1 DNA helicase inhibits both telomerase-mediated telomere lengthening and de novo telomere addition at double strand breaks (..
  51. Sakofsky C, Ayyar S, Deem A, Chung W, Ira G, Malkova A. Translesion Polymerases Drive Microhomology-Mediated Break-Induced Replication Leading to Complex Chromosomal Rearrangements. Mol Cell. 2015;60:860-72 pubmed publisher
    ..of homology-driven break-induced replication (BIR) caused by defective repair DNA synthesis in the absence of Pif1 helicase leads to template switches involving 0-6 nt of homology, followed by resolution of recombination ..
  52. Strecker J, Stinus S, Caballero M, Szilard R, Chang M, Durocher D. A sharp Pif1-dependent threshold separates DNA double-strand breaks from critically short telomeres. elife. 2017;6: pubmed publisher
    ..In Saccharomyces cerevisiae, the Pif1 helicase, a telomerase inhibitor, lies at the interface of these end-fate decisions...
  53. Tran P, Pohl T, Chen C, Chan A, Pott S, Zakian V. PIF1 family DNA helicases suppress R-loop mediated genome instability at tRNA genes. Nat Commun. 2017;8:15025 pubmed publisher
    Saccharomyces cerevisiae encodes two Pif1 family DNA helicases, Pif1 and Rrm3. Rrm3 promotes DNA replication past stable protein complexes at tRNA genes (tDNAs)...
  54. Geronimo C, Zakian V. Getting it done at the ends: Pif1 family DNA helicases and telomeres. DNA Repair (Amst). 2016;44:151-158 pubmed publisher
    ..This review focuses on one such class of DNA helicases, the Pif1 family of 5'-3' DNA helicases...
  55. Rossi S, Carotenuto W, Giannattasio M. Genome-wide localization of Rrm3 and Pif1 DNA helicases at stalled active and inactive DNA replication forks of Saccharomyces cerevisiae. Genom Data. 2016;7:162-5 pubmed publisher
    ..We present datasets deposited in the gene expression omnibus (GEO) database under accession number GSE68214, which show how the DNA helicases Rrm3 and Pif1 (8) associate to active and inactive DNA replication forks.
  56. Chib S, Byrd A, Raney K. Yeast Helicase Pif1 Unwinds RNA:DNA Hybrids with Higher Processivity than DNA:DNA Duplexes. J Biol Chem. 2016;291:5889-901 pubmed publisher
    Saccharomyces cerevisiae Pif1, an SF1B helicase, has been implicated in both mitochondrial and nuclear functions...
  57. Zhou R, Zhang J, Bochman M, Zakian V, Ha T. Periodic DNA patrolling underlies diverse functions of Pif1 on R-loops and G-rich DNA. elife. 2014;3:e02190 pubmed
    b>Pif1 family helicases are conserved from bacteria to humans...
  58. Henry R, Balakrishnan L, Ying Lin S, Campbell J, Bambara R. Components of the secondary pathway stimulate the primary pathway of eukaryotic Okazaki fragment processing. J Biol Chem. 2010;285:28496-505 pubmed publisher
    ..The flaps must then be cleaved by Dna2 nuclease/helicase before FEN1 can act. Pif1 helicase aids creation of long flaps...
  59. Banerjee S, Smith S, Oum J, Liaw H, Hwang J, Sikdar N, et al. Mph1p promotes gross chromosomal rearrangement through partial inhibition of homologous recombination. J Cell Biol. 2008;181:1083-93 pubmed publisher
    ..Furthermore, spontaneous RPA foci at DSBs are destabilized by the mph1Delta mutation. Therefore, Mph1p promotes GCR formation by partially suppressing HR, likely through its interaction with RPA. ..
  60. Ouenzar F, Lalonde M, Laprade H, Morin G, Gallardo F, Tremblay Belzile S, et al. Cell cycle-dependent spatial segregation of telomerase from sites of DNA damage. J Cell Biol. 2017;216:2355-2371 pubmed publisher
    ..Although TLC1 RNA accumulates in the nucleoplasm in G1/S, Pif1 activity promotes TLC1 RNA localization in the nucleolus in G2/M...
  61. Ramanagoudr Bhojappa R, Blair L, Tackett A, Raney K. Physical and functional interaction between yeast Pif1 helicase and Rim1 single-stranded DNA binding protein. Nucleic Acids Res. 2013;41:1029-46 pubmed publisher
    b>Pif1 helicase plays various roles in the maintenance of nuclear and mitochondrial genome integrity in most eukaryotes...
  62. Stundon J, Zakian V. Identification of Saccharomyces cerevisiae Genes Whose Deletion Causes Synthetic Effects in Cells with Reduced Levels of the Nuclear Pif1 DNA Helicase. G3 (Bethesda). 2015;5:2913-8 pubmed publisher
    The multifunctional Saccharomyces cerevisiae Pif1 DNA helicase affects the maintenance of telomeric, ribosomal, and mitochondrial DNAs, suppresses DNA damage at G-quadruplex motifs, influences the processing of Okazaki fragments, and ..
  63. Rossi S, Ajazi A, Carotenuto W, Foiani M, Giannattasio M. Rad53-Mediated Regulation of Rrm3 and Pif1 DNA Helicases Contributes to Prevention of Aberrant Fork Transitions under Replication Stress. Cell Rep. 2015;13:80-92 pubmed publisher
    ..Here, we show that Rrm3 and Pif1, DNA helicases assisting fork progression across pausing sites, are detrimental in rad53 mutants experiencing ..
  64. Koc K, Singh S, Stodola J, Burgers P, Galletto R. Pif1 removes a Rap1-dependent barrier to the strand displacement activity of DNA polymerase ?. Nucleic Acids Res. 2016;44:3811-9 pubmed publisher
    ..To this end, we show that Pif1, a 5'-3' helicase, not only stimulates the strand displacement activity of Pol ? but it also allows efficient ..
  65. Banerjee S, Myung K. Increased genome instability and telomere length in the elg1-deficient Saccharomyces cerevisiae mutant are regulated by S-phase checkpoints. Eukaryot Cell. 2004;3:1557-66 pubmed
    ..size independently of other previously known telomere maintenance proteins such as the telomerase inhibitor Pif1 or the telomere size regulator Rif1...
  66. Ferreira H, Luke B, Schober H, Kalck V, Lingner J, Gasser S. The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast. Nat Cell Biol. 2011;13:867-74 pubmed publisher
    ..Siz2 also provokes telomere extension in a telomerase-dependent manner that is epistatic with loss of the helicase Pif1. Consistent with our previously documented role for telomerase in anchorage, normal telomere anchoring in siz2 ? is ..