Gene Symbol: PHO84
Description: phosphate transporter PHO84
Alias: phosphate transporter PHO84
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Jensen L, Ajua Alemanji M, Culotta V. The Saccharomyces cerevisiae high affinity phosphate transporter encoded by PHO84 also functions in manganese homeostasis. J Biol Chem. 2003;278:42036-40 pubmed
    ..In a search for other genes involved in manganese homeostasis, PHO84 was identified...
  2. Holbein S, Freimoser F, Werner T, Wengi A, Dichtl B. Cordycepin-hypersensitive growth links elevated polyphosphate levels to inhibition of poly(A) polymerase in Saccharomyces cerevisiae. Nucleic Acids Res. 2008;36:353-63 pubmed
    ..Based on these observations, we suggest that binding of poly P to poly(A) polymerase negatively regulates its activity. ..
  3. Rosenfeld L, Reddi A, Leung E, Aranda K, Jensen L, Culotta V. The effect of phosphate accumulation on metal ion homeostasis in Saccharomyces cerevisiae. J Biol Inorg Chem. 2010;15:1051-62 pubmed publisher
    ..Overall, these studies underscore the importance of maintaining a charge balance in the cell; a disruption in phosphate metabolism can dramatically impact on metal homeostasis. ..
  4. Arino J. Integrative responses to high pH stress in S. cerevisiae. OMICS. 2010;14:517-23 pubmed publisher
    ..In this review we will examine these responses, their possible interactions, and the role that they play in tolerance to high pH stress. ..
  5. Bun Ya M, Nishimura M, Harashima S, Oshima Y. The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter. Mol Cell Biol. 1991;11:3229-38 pubmed
    The PHO84 gene specifies Pi-transport in Saccharomyces cerevisiae. A DNA fragment bearing the PHO84 gene was cloned by its ability to complement constitutive synthesis of repressible acid phosphatase of pho84 mutant cells...
  6. Ghillebert R, Swinnen E, De Snijder P, Smets B, Winderickx J. Differential roles for the low-affinity phosphate transporters Pho87 and Pho90 in Saccharomyces cerevisiae. Biochem J. 2011;434:243-51 pubmed publisher
    ..pathway, wherein the Pho4 transcription factor mediates expression of genes involved in P(i) acquisition, such as PHO84, encoding the high-affinity H(+)/P(i) symporter...
  7. Lazard M, Blanquet S, Fisicaro P, Labarraque G, Plateau P. Uptake of selenite by Saccharomyces cerevisiae involves the high and low affinity orthophosphate transporters. J Biol Chem. 2010;285:32029-37 pubmed publisher
    ..The properties of phosphate and selenite transport enable us to propose an explanation to the paradoxical increase of selenite toxicity when phosphate concentration in the growth medium is raised above 1 mm. ..
  8. Wykoff D, O Shea E. Phosphate transport and sensing in Saccharomyces cerevisiae. Genetics. 2001;159:1491-9 pubmed
    ..These data suggest that PHO84 is not required for sensing phosphate...
  9. O Connell K, Baker R. Possible cross-regulation of phosphate and sulfate metabolism in Saccharomyces cerevisiae. Genetics. 1992;132:63-73 pubmed
    ..Subcloning experiments confirmed that suppression correlated with increased dosage of PHO4. PHO4c, pho80 and pho84 mutations, all of which lead to constitutive activation of the PHO4 transcription factor, also suppressed cep1 ..

More Information


  1. Serra Cardona A, Petrezsélyová S, Canadell D, Ramos J, Ariño J. Coregulated expression of the Na+/phosphate Pho89 transporter and Ena1 Na+-ATPase allows their functional coupling under high-pH stress. Mol Cell Biol. 2014;34:4420-35 pubmed publisher
    The yeast Saccharomyces cerevisiae has two main high-affinity inorganic phosphate (Pi) transporters, Pho84 and Pho89, that are functionally relevant at acidic/neutral pH and alkaline pH, respectively...
  2. Jiang J, Stumpferl S, Tiwari A, Qin Q, Rodríguez Quiñones J, Jazwinski S. Identification of the Target of the Retrograde Response that Mediates Replicative Lifespan Extension in Saccharomyces cerevisiae. Genetics. 2016;204:659-673 pubmed
    ..Of the four, only deletion of PHO84 suppressed lifespan extension. Retrograde-response activation of PHO84 displayed some preference for SAGA...
  3. Springer M, Wykoff D, Miller N, O Shea E. Partially phosphorylated Pho4 activates transcription of a subset of phosphate-responsive genes. PLoS Biol. 2003;1:E28 pubmed
    ..Differential phosphorylation of Pho4 by Pho80-Pho85 produces phosphorylated forms of Pho4 that differ in their ability to activate transcription, contributing to multiple outputs. ..
  4. Yompakdee C, Bun Ya M, Shikata K, Ogawa N, Harashima S, Oshima Y. A putative new membrane protein, Pho86p, in the inorganic phosphate uptake system of Saccharomyces cerevisiae. Gene. 1996;171:41-7 pubmed
    The PHO84 gene in Saccharomyces cerevisiae encodes the P(i) transporter Pho84p. The other three genes, GTR1, PHO86 and PHO87, are also suggested to be involved in the P(i) uptake system...
  5. Pimentel C, Caetano S, Menezes R, Figueira I, Santos C, Ferreira R, et al. Yap1 mediates tolerance to cobalt toxicity in the yeast Saccharomyces cerevisiae. Biochim Biophys Acta. 2014;1840:1977-86 pubmed publisher
    ..Cobalt has a rare occurrence in nature, but may accumulate in cells to toxic levels. In the present study, we have investigated how the transcription factor Yap1 mediates tolerance to cobalt toxicity...
  6. Castelnuovo M, Rahman S, Guffanti E, Infantino V, Stutz F, Zenklusen D. Bimodal expression of PHO84 is modulated by early termination of antisense transcription. Nat Struct Mol Biol. 2013;20:851-8 pubmed publisher
    ..Loss of Rrp6 results in the accumulation of long PHO84 antisense (AS) RNAs and repression of sense transcription through PHO84 promoter deacetylation...
  7. Popova Y, Thayumanavan P, Lonati E, Agrochão M, Thevelein J. Transport and signaling through the phosphate-binding site of the yeast Pho84 phosphate transceptor. Proc Natl Acad Sci U S A. 2010;107:2890-5 pubmed publisher
    ..The action mechanism of these "transceptors" is unclear. The Pho84 phosphate transceptor in yeast transports phosphate and mediates rapid phosphate activation of the protein kinase ..
  8. Bun Ya M, Shikata K, Nakade S, Yompakdee C, Harashima S, Oshima Y. Two new genes, PHO86 and PHO87, involved in inorganic phosphate uptake in Saccharomyces cerevisiae. Curr Genet. 1996;29:344-51 pubmed
    The PHO84 gene in Saccharomyces cerevisiae encodes a Pi transporter, mutation of which confers constitutive synthesis of repressible acid phosphatase (rAPase), in medium containing repressible amounts of Pi, and an arsenate-resistant ..
  9. Ceasar S, Baker A, Muench S, Ignacimuthu S, Baldwin S. The conservation of phosphate-binding residues among PHT1 transporters suggests that distinct transport affinities are unlikely to result from differences in the phosphate-binding site. Biochem Soc Trans. 2016;44:1541-1548 pubmed residues were shown to be well conserved between all the plant PHT1 proteins, Saccharomyces cerevisiae PHO84 and PiPT...
  10. Wongwisansri S, Laybourn P. Disruption of histone deacetylase gene RPD3 accelerates PHO5 activation kinetics through inappropriate Pho84p recycling. Eukaryot Cell. 2005;4:1387-95 pubmed
    ..We propose that Rpd3p has a negative role in the regulation of Pho84p endocytosis. ..
  11. Samyn D, Ruiz Pavón L, Andersson M, Popova Y, Thevelein J, Persson B. Mutational analysis of putative phosphate- and proton-binding sites in the Saccharomyces cerevisiae Pho84 phosphate:H(+) transceptor and its effect on signalling to the PKA and PHO pathways. Biochem J. 2012;445:413-22 pubmed publisher
    In Saccharomyces cerevisiae, the Pho84 phosphate transporter acts as the main provider of phosphate to the cell using a proton symport mechanism, but also mediates rapid activation of the PKA (protein kinase A) pathway...
  12. Perlstein E, Ruderfer D, Roberts D, Schreiber S, Kruglyak L. Genetic basis of individual differences in the response to small-molecule drugs in yeast. Nat Genet. 2007;39:496-502 pubmed therapeutic calcium channel blockers, and that a natural coding-region polymorphism in the inorganic phosphate transporter PHO84 underlies sensitivity to two polychlorinated phenols that uncouple oxidative phosphorylation...
  13. Yompakdee C, Ogawa N, Harashima S, Oshima Y. A putative membrane protein, Pho88p, involved in inorganic phosphate transport in Saccharomyces cerevisiae. Mol Gen Genet. 1996;251:580-90 pubmed
    ..Both disruption and high dosage of PHO88 or PHO86 resulted in reduced Pi uptake. These findings suggest that Pho88p is also involved in Pi transport and modulates Pho81p function together with Pho86p. ..
  14. Pérez Sampietro M, Casas C, Herrero E. The AMPK family member Snf1 protects Saccharomyces cerevisiae cells upon glutathione oxidation. PLoS ONE. 2013;8:e58283 pubmed publisher
  15. Teunissen J, Crooijmans M, Teunisse P, Van Heusden G. Lack of 14-3-3 proteins in Saccharomyces cerevisiae results in cell-to-cell heterogeneity in the expression of Pho4-regulated genes SPL2 and PHO84. BMC Genomics. 2017;18:701 pubmed publisher
    ..analysis of the effect of disruption of either BMH1 or BMH2 revealed that the mRNA levels of the PHO genes PHO84 and SPL2 are greatly reduced in the mutant strains compared to the levels in wild type strains...
  16. Tomar P, Sinha H. Conservation of PHO pathway in ascomycetes and the role of Pho84. J Biosci. 2014;39:525-36 pubmed
    ..The role of high-affinity transporter, Pho84, in sensing and signalling of phosphate has also been discussed.
  17. Samyn D, Van der Veken J, Van Zeebroeck G, Persson B, Karlsson B. Key Residues and Phosphate Release Routes in the Saccharomyces cerevisiae Pho84 Transceptor: THE ROLE OF TYR179 IN FUNCTIONAL REGULATION. J Biol Chem. 2016;291:26388-26398 pubmed
    b>Pho84, a major facilitator superfamily (MFS) protein, is the main high-affinity Pi transceptor in Saccharomyces cerevisiae Although transport mechanisms have been suggested for other MFS members, the key residues and molecular ..
  18. Reddi A, Culotta V. Regulation of manganese antioxidants by nutrient sensing pathways in Saccharomyces cerevisiae. Genetics. 2011;189:1261-70 pubmed publisher
    ..Our observed roles for nutrient and stress response kinases and transcription factors in regulating the Mn antioxidant underscore its physiological importance in aerobic fitness. ..
  19. Shen M, Shah D, Chen W, Da Silva N. Enhanced arsenate uptake in Saccharomyces cerevisiae overexpressing the Pho84 phosphate transporter. Biotechnol Prog. 2012;28:654-61 pubmed publisher
    ..The phosphate transporter, Pho84p, known to import arsenate, was overexpressed using a 2?-based vector carrying PHO84 under the control of the late-phase ADH2 promoter. Arsenate uptake was then evaluated using a resting cell system...
  20. Lundh F, Mouillon J, Samyn D, Stadler K, Popova Y, Lagerstedt J, et al. Molecular mechanisms controlling phosphate-induced downregulation of the yeast Pho84 phosphate transporter. Biochemistry. 2009;48:4497-505 pubmed publisher
    In Saccharomyces cerevisiae, phosphate uptake is mainly dependent on the proton-coupled Pho84 permease under phosphate-limited growth conditions...
  21. Hürlimann H, Stadler Waibel M, Werner T, Freimoser F. Pho91 Is a vacuolar phosphate transporter that regulates phosphate and polyphosphate metabolism in Saccharomyces cerevisiae. Mol Biol Cell. 2007;18:4438-45 pubmed
    ..using poly P content as a read-out, it was possible to define novel functions of the five phosphate transporters: Pho84, Pho87, Pho89, Pho90, and Pho91, in budding yeast...
  22. Jonsson Z, Jha S, Wohlschlegel J, Dutta A. Rvb1p/Rvb2p recruit Arp5p and assemble a functional Ino80 chromatin remodeling complex. Mol Cell. 2004;16:465-77 pubmed Rvb2p associates with Arp5p in vitro in a reaction dependent on the presence of ATP and Ino80p. Therefore, Rvbp are required for the structural and functional integrity of the Ino80 chromatin remodeling complex. ..
  23. Rosenfeld L, Culotta V. Phosphate disruption and metal toxicity in Saccharomyces cerevisiae: effects of RAD23 and the histone chaperone HPC2. Biochem Biophys Res Commun. 2012;418:414-9 pubmed publisher
    ..chaperone gene HPC2 reversed the elevated manganese and phosphate of pho80 mutants by specifically repressing PHO84, encoding a metal-phosphate transporter...
  24. Puigpinós J, Casas C, Herrero E. Altered intracellular calcium homeostasis and endoplasmic reticulum redox state in Saccharomyces cerevisiae cells lacking Grx6 glutaredoxin. Mol Biol Cell. 2015;26:104-16 pubmed publisher
    ..The results presented in this study provide evidence for redox regulation of calcium homeostasis in yeast cells. ..
  25. Ofiteru A, Ruta L, Rotaru C, Dumitru I, Ene C, Neagoe A, et al. Overexpression of the PHO84 gene causes heavy metal accumulation and induces Ire1p-dependent unfolded protein response in Saccharomyces cerevisiae cells. Appl Microbiol Biotechnol. 2012;94:425-35 pubmed publisher
    ..In the present study, the effect of PHO84 overexpression upon the heavy metal accumulation by yeast cells was investigated...
  26. Wippo C, Krstulovic B, Ertel F, Musladin S, Blaschke D, Stürzl S, et al. Differential cofactor requirements for histone eviction from two nucleosomes at the yeast PHO84 promoter are determined by intrinsic nucleosome stability. Mol Cell Biol. 2009;29:2960-81 pubmed publisher
    ..In this study we asked if chromatin remodeling at the even stronger PHO84 promoter was correspondingly less cofactor dependent...
  27. Berhe A, Fristedt U, Persson B. Expression and purification of the high-affinity phosphate transporter of Saccharomyces cerevisiae. Eur J Biochem. 1995;227:566-72 pubmed
    ..Approximately 5 mg purified His10-permease was obtained from 3 g (wet mass) cells. The purified phosphate permease chimera catalyzes uncoupler-sensitive phosphate transport after reconstitution into proteoliposomes. ..