PHO2

Summary

Gene Symbol: PHO2
Description: Pho2p
Alias: BAS2, GRF10, Pho2p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Komeili A, O Shea E. Roles of phosphorylation sites in regulating activity of the transcription factor Pho4. Science. 1999;284:977-80 pubmed
    ..Phosphorylation of a fourth site blocks the interaction of Pho4 with the transcription factor Pho2. Multiple phosphorylation sites provide overlapping and partially redundant layers of regulation that function to ..
  2. Hirst K, Fisher F, McAndrew P, Goding C. The transcription factor, the Cdk, its cyclin and their regulator: directing the transcriptional response to a nutritional signal. EMBO J. 1994;13:5410-20 pubmed
    ..We show here that Pho4 and the homeobox protein Pho2 interact in vivo and act cooperatively to activate the PHO5 UAS, with interaction being regulated by the phosphate ..
  3. Sung M, Huh W. In vivo quantification of protein-protein interactions in Saccharomyces cerevisiae using bimolecular fluorescence complementation assay. J Microbiol Methods. 2010;83:194-201 pubmed publisher
  4. McBride H, Brazas R, Yu Y, Nasmyth K, Stillman D. Long-range interactions at the HO promoter. Mol Cell Biol. 1997;17:2669-78 pubmed
    ..been investigated in some detail, and we have shown that Swi5p binds site B in a mutually cooperative fashion with Pho2p, a homeodomain protein...
  5. Nourani A, Utley R, Allard S, Cote J. Recruitment of the NuA4 complex poises the PHO5 promoter for chromatin remodeling and activation. EMBO J. 2004;23:2597-607 pubmed
    ..Two DNA-binding proteins Pho2 and Pho4 are critical for this step...
  6. Shao D, Creasy C, Bergman L. Interaction of Saccharomyces cerevisiae Pho2 with Pho4 increases the accessibility of the activation domain of Pho4. Mol Gen Genet. 1996;251:358-64 pubmed
    ..encoded by the PHO5 gene, requires two positive regulatory factors, Pho4 and Pho2 (also called Bas2 or Grf10)...
  7. Rossmann M, Luo W, Tsaponina O, Chabes A, Stillman B. A common telomeric gene silencing assay is affected by nucleotide metabolism. Mol Cell. 2011;42:127-36 pubmed publisher
    ..We conclude that metabolic changes caused by the drug employed and certain mutants being studied are incompatible with the use of certain prototrophic markers for TPEV. ..
  8. Zhou X, O Shea E. Integrated approaches reveal determinants of genome-wide binding and function of the transcription factor Pho4. Mol Cell. 2011;42:826-36 pubmed publisher
    ..Pho4 binding is not sufficient for transcriptional activation-a cooperative interaction between Pho2 and Pho4 specifies genes that are activated...
  9. Vogel K, Hörz W, Hinnen A. The two positively acting regulatory proteins PHO2 and PHO4 physically interact with PHO5 upstream activation regions. Mol Cell Biol. 1989;9:2050-7 pubmed
    ..acid phosphatase gene PHO5 of Saccharomyces cerevisiae requires the two positively acting regulatory proteins PHO2 and PHO4 for expression. pho2 or pho4 mutants are not able to derepress the PHO5 gene under low-Pi conditions...

More Information

Publications35

  1. Lemire J, Willcocks T, Halvorson H, Bostian K. Regulation of repressible acid phosphatase gene transcription in Saccharomyces cerevisiae. Mol Cell Biol. 1985;5:2131-41 pubmed
    ..These mutants identify numerous positive- (PHO4, PHO2, PHO81) and negative-acting (PHO80, PHO85) regulatory loci dispersed throughout the yeast genome...
  2. Subramanian M, Qiao W, Khanam N, Wilkins O, Der S, Lalich J, et al. Transcriptional regulation of the one-carbon metabolism regulon in Saccharomyces cerevisiae by Bas1p. Mol Microbiol. 2005;57:53-69 pubmed
    ..of these genes in response to glycine removal but this phenotype was much less apparent in the absence of BAS2 or GCN4...
  3. Pinson B, Gabrielsen O, Daignan Fornier B. Redox regulation of AMP synthesis in yeast: a role of the Bas1p and Bas2p transcription factors. Mol Microbiol. 2000;36:1460-9 pubmed
    ..This hypothesis was further substantiated in a two hybrid experiment showing that Bas1p/Bas2p interaction is affected by oxidative stress. ..
  4. Pinson B, Kongsrud T, Ording E, Johansen L, Daignan Fornier B, Gabrielsen O. Signaling through regulated transcription factor interaction: mapping of a regulatory interaction domain in the Myb-related Bas1p. Nucleic Acids Res. 2000;28:4665-73 pubmed
    ..Bas1p is a Myb-related transcription factor that acts together with the homeodomain-related Bas2p (Pho2p) to regulate purine and histidine biosynthesis genes in response to extracellular purine limitation...
  5. Berben G, Legrain M, Hilger F. Studies on the structure, expression and function of the yeast regulatory gene PHO2. Gene. 1988;66:307-12 pubmed
    The yeast regulatory gene PHO2 encodes a protein assumed to activate, in concert with the PHO4 protein, the transcription of the repressible acid phosphatase-coding gene PHO5. The PHO2 gene was cloned and sequenced...
  6. Xia Z, Ao S. Analysis of Interaction between PHO4 and PHO2 Protein by Real Time BIA. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1999;31:145-149 pubmed
    Applying real time BIA(biomolecular interaction analysis) the interaction between yeast PHO4 and PHO2 protein was analyzed. Recombinant PHO4 protein was coupled at the sensor chip via amino group...
  7. Springer M, Wykoff D, Miller N, O Shea E. Partially phosphorylated Pho4 activates transcription of a subset of phosphate-responsive genes. PLoS Biol. 2003;1:E28 pubmed
    ..Differential phosphorylation of Pho4 by Pho80-Pho85 produces phosphorylated forms of Pho4 that differ in their ability to activate transcription, contributing to multiple outputs. ..
  8. Bhoite L, Stillman D. Residues in the Swi5 zinc finger protein that mediate cooperative DNA binding with the Pho2 homeodomain protein. Mol Cell Biol. 1998;18:6436-46 pubmed
    ..Pho2 (also known as Bas2 or Grf10) activates transcription of diverse genes, acting with multiple distinct DNA-binding proteins...
  9. Fascher K, Schmitz J, Hörz W. Role of trans-activating proteins in the generation of active chromatin at the PHO5 promoter in S. cerevisiae. EMBO J. 1990;9:2523-8 pubmed
    ..We have now investigated the role of two trans-activating proteins, encoded by PHO2 and PHO4, which bind to the PHO5 promoter...
  10. Yang J, Ao S. Interaction of the Yeast PH02 Protein or Its Mutants with the PHO5 UAS in vitro. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1996;28:316-320 pubmed
    The GST gene fusion system was used to express PHO2 gene and its mutants in E. coli Gel retardation assays showed that PHO2 fusion protein can bind to the upstream activation sequence (UAS) of the acid phosphatase gene PHO5...
  11. Rebora K, Desmoucelles C, Borne F, Pinson B, Daignan Fornier B. Yeast AMP pathway genes respond to adenine through regulated synthesis of a metabolic intermediate. Mol Cell Biol. 2001;21:7901-12 pubmed
    ..genes (ADE genes) are transcriptionally activated in the absence of extracellular purines by the Bas1p and Bas2p (Pho2p) transcription factors...
  12. Barbaric S, Munsterkotter M, Goding C, Hörz W. Cooperative Pho2-Pho4 interactions at the PHO5 promoter are critical for binding of Pho4 to UASp1 and for efficient transactivation by Pho4 at UASp2. Mol Cell Biol. 1998;18:2629-39 pubmed
    ..depends on two transcriptional activators, the basic helix-loop-helix protein Pho4 and the homeodomain protein Pho2. Pho4 acts through two essential binding sites corresponding to the regulatory elements UASp1 and UASp2...
  13. Magbanua J, Ogawa N, Harashima S, Oshima Y. The transcriptional activators of the PHO regulon, Pho4p and Pho2p, interact directly with each other and with components of the basal transcription machinery in Saccharomyces cerevisiae. J Biochem. 1997;121:1182-9 pubmed
    The transcriptional regulators Pho4p and Pho2p are involved in transcription of several genes in the PHO regulon of Saccharomyces cerevisiae...
  14. Pinson B, Vaur S, Sagot I, Coulpier F, Lemoine S, Daignan Fornier B. Metabolic intermediates selectively stimulate transcription factor interaction and modulate phosphate and purine pathways. Genes Dev. 2009;23:1399-407 pubmed publisher
    ..The transcription factor Pho2p is required for up-regulation of all AICAR-responsive genes...
  15. Som I, Mitsch R, Urbanowski J, Rolfes R. DNA-bound Bas1 recruits Pho2 to activate ADE genes in Saccharomyces cerevisiae. Eukaryot Cell. 2005;4:1725-35 pubmed
    ..Derepression requires the transcriptional activators Bas1 and Pho2, as well as the biosynthetic intermediates 5'-phosphoribosyl-4-succinocarboxamide-5-aminoimidazole (SAICAR) and 5'-..
  16. Yang J, Wu J, Ao S. Analysis of Activation Activity of Yeast PHO2, PHO4 Protein and Their Interaction. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1997;29:122-128 pubmed
    Both PHO2 and PHO4 are positive regulatory factors of yeast PHO5 gene...
  17. Hannum C, Kulaeva O, Sun H, Urbanowski J, Wendus A, Stillman D, et al. Functional mapping of Bas2. Identification of activation and Bas1-interaction domains. J Biol Chem. 2002;277:34003-9 pubmed
    The transcriptional activator protein Bas2 is required to express more than 20 genes in pathways for purine nucleotide and histidine biosynthesis, phosphate utilization, and the HO endonuclease by acting with co-regulator proteins Bas1, ..
  18. Mutvei A, Dihlmann S, Herth W, Hurt E. NSP1 depletion in yeast affects nuclear pore formation and nuclear accumulation. Eur J Cell Biol. 1992;59:280-95 pubmed
    ..During NSP1 depletion, nuclear accumulation of two reporter proteins Mat alpha 2-lacZ and PHO2-lacZ is inhibited, and the chimeric proteins appear in the cytoplasm of GAL::nsp1 cells...
  19. Brazas R, Stillman D. Identification and purification of a protein that binds DNA cooperatively with the yeast SWI5 protein. Mol Cell Biol. 1993;13:5524-37 pubmed
    ..A yeast factor, GRF10 (also known as PHO2 and BAS2), that promoted high-affinity binding of SWI5 in the presence of a large excess of ..
  20. Brazas R, Stillman D. The Swi5 zinc-finger and Grf10 homeodomain proteins bind DNA cooperatively at the yeast HO promoter. Proc Natl Acad Sci U S A. 1993;90:11237-41 pubmed
    ..N-terminal amino acid sequence analysis identified the Swi5 stimulatory factor as the product of the GRF10 gene, which encodes a yeast homeodomain protein...
  21. Martinez J, Bordel S, Hong K, Nielsen J. Gcn4p and the Crabtree effect of yeast: drawing the causal model of the Crabtree effect in Saccharomyces cerevisiae and explaining evolutionary trade-offs of adaptation to galactose through systems biology. FEMS Yeast Res. 2014;14:654-62 pubmed publisher
    ..cerevisiae strains growing on galactose and glucose, it was inferred that the transcription factors Bas1p, Pho2p, and Gcn4p play a central role in the regulatory events causing the Crabtree effect in S. cerevisiae...
  22. Liu C, Yang Z, Yang J, Xia Z, Ao S. Regulation of the yeast transcriptional factor PHO2 activity by phosphorylation. J Biol Chem. 2000;275:31972-8 pubmed
    The induction of yeast Saccharomyces cerevisiae gene PHO5 expression is mediated by transcriptional factors PHO2 and PHO4...
  23. Holbein S, Freimoser F, Werner T, Wengi A, Dichtl B. Cordycepin-hypersensitive growth links elevated polyphosphate levels to inhibition of poly(A) polymerase in Saccharomyces cerevisiae. Nucleic Acids Res. 2008;36:353-63 pubmed
    ..Based on these observations, we suggest that binding of poly P to poly(A) polymerase negatively regulates its activity. ..
  24. Brazas R, Bhoite L, Murphy M, Yu Y, Chen Y, Neklason D, et al. Determining the requirements for cooperative DNA binding by Swi5p and Pho2p (Grf10p/Bas2p) at the HO promoter. J Biol Chem. 1995;270:29151-61 pubmed
    ..In vitro biochemical studies using purified Swi5p and Pho2p proteins have demonstrated that Swi5p and Pho2p bind cooperatively to the HO promoter...
  25. Arndt K, Styles C, Fink G. Multiple global regulators control HIS4 transcription in yeast. Science. 1987;237:874-80 pubmed
    ..The basal level transcription of the HIS4 gene is under the control of two genes, BAS1 and BAS2, which are also required for the control of purine biosynthesis...
  26. Daignan Fornier B, Fink G. Coregulation of purine and histidine biosynthesis by the transcriptional activators BAS1 and BAS2. Proc Natl Acad Sci U S A. 1992;89:6746-50 pubmed
    ..The transcription factors BAS1 and BAS2/PHO2, which are also regulators of the histidine pathway, participate in the regulation of the purine biosynthetic ..