Gene Symbol: PFK2
Description: 6-phosphofructokinase subunit beta
Alias: 6-phosphofructokinase subunit beta
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Heinisch J. Construction and physiological characterization of mutants disrupted in the phosphofructokinase genes of Saccharomyces cerevisiae. Curr Genet. 1986;11:227-34 pubmed
    ..the alpha-subunits were not detectable in pfk1-disruptions whereas the beta-subunits were undetectable in pfk2-disruptions...
  2. Herrmann K, Diezel W, Kopperschlager G, Hofmann E. Immunological evidence for non-identical subunits in yeast phosphofructokinase. FEBS Lett. 1973;36:190-2 pubmed
  3. Kamber R, Shoemaker C, Denic V. Receptor-Bound Targets of Selective Autophagy Use a Scaffold Protein to Activate the Atg1 Kinase. Mol Cell. 2015;59:372-81 pubmed publisher
    ..This regulatory logic is a key similarity between selective autophagy and bulk autophagy, which is initiated by a distinct Atg1 activation mechanism during starvation. ..
  4. Aiello D, Fu L, Miseta A, Bedwell D. Intracellular glucose 1-phosphate and glucose 6-phosphate levels modulate Ca2+ homeostasis in Saccharomyces cerevisiae. J Biol Chem. 2002;277:45751-8 pubmed
    ..These results provide strong evidence that cellular Ca(2+) homeostasis is coupled to the relative levels of Glc-6-P and Glc-1-P in yeast. ..
  5. Bonfils G, Jaquenoud M, Bontron S, Ostrowicz C, Ungermann C, De Virgilio C. Leucyl-tRNA synthetase controls TORC1 via the EGO complex. Mol Cell. 2012;46:105-10 pubmed publisher
    ..Thus, the EGOC-TORC1 signaling module samples, via the LeuRS-intrinsic editing domain, the fidelity of tRNA(Leu) aminoacylation as a proxy for leucine availability. ..
  6. Heinisch J. Isolation and characterization of the two structural genes coding for phosphofructokinase in yeast. Mol Gen Genet. 1986;202:75-82 pubmed
    ..The genes coding for these subunits have been isolated by means of functional complementation in a pfk1 pfk2 double mutant...
  7. Chaffotte A, Laurent M, Tijane M, Tardieu A, Roucous C, Seydoux F, et al. Studies on the structure of yeast phosphofructokinase. Biochimie. 1984;66:49-58 pubmed
    ..In particular, our experiments show that the reliability of molecular weight determinations performed by gel filtration of native proteins must be considered with caution. ..
  8. Chan C, Parra K. Yeast phosphofructokinase-1 subunit Pfk2p is necessary for pH homeostasis and glucose-dependent vacuolar ATPase reassembly. J Biol Chem. 2014;289:19448-57 pubmed publisher
    ..The pfk1? and pfk2? mutants grew on glucose and assembled wild-type levels of V-ATPase pumps at the membrane...
  9. Brandina I, Graham J, Lemaitre Guillier C, Entelis N, Krasheninnikov I, Sweetlove L, et al. Enolase takes part in a macromolecular complex associated to mitochondria in yeast. Biochim Biophys Acta. 2006;1757:1217-28 pubmed
    ..This suggests an unsuspected novel function for this complex in tRNA mitochondrial import. ..

More Information


  1. Barnard E, McFerran N, Trudgett A, Nelson J, Timson D. Detection and localisation of protein-protein interactions in Saccharomyces cerevisiae using a split-GFP method. Fungal Genet Biol. 2008;45:597-604 pubmed publisher
  2. Su Y, Blake Palmer K, Sorrell S, Javid B, Bowers K, Zhou A, et al. Human H+ATPase a4 subunit mutations causing renal tubular acidosis reveal a role for interaction with phosphofructokinase-1. Am J Physiol Renal Physiol. 2008;295:F950-8 pubmed publisher
    ..Thus in the context of dRTA, stability and function of the metabolon composed of H+ATPase and glycolytic components can be compromised by either loss of required PFK-1 binding (G820R) or loss of pump protein (R807Q). ..
  3. Scherrer T, Mittal N, Janga S, Gerber A. A screen for RNA-binding proteins in yeast indicates dual functions for many enzymes. PLoS ONE. 2010;5:e15499 pubmed publisher
    ..Our results suggest that many proteins may associate with mRNAs and possibly control their fates, providing dense connections between different layers of cellular regulation. ..
  4. Chan C, Dominguez D, Parra K. Regulation of Vacuolar H+-ATPase (V-ATPase) Reassembly by Glycolysis Flow in 6-Phosphofructo-1-kinase (PFK-1)-deficient Yeast Cells. J Biol Chem. 2016;291:15820-9 pubmed publisher
    ..Chem. 289, 19448-19457). This study capitalized on the mechanisms suppressing vacuolar H(+)-ATPase (V-ATPase) in pfk2? to gain new knowledge of the mechanisms underlying glucose-dependent V-ATPase regulation...
  5. Banaszak K, Mechin I, Obmolova G, Oldham M, Chang S, Ruiz T, et al. The crystal structures of eukaryotic phosphofructokinases from baker's yeast and rabbit skeletal muscle. J Mol Biol. 2011;407:284-97 pubmed publisher
    ..Crystallographic results have been used as the basis for structure-based effector design. ..
  6. Huang D, Wilson W, Roach P. Glucose-6-P control of glycogen synthase phosphorylation in yeast. J Biol Chem. 1997;272:22495-501 pubmed
    ..From a genetic screen, we have found that mutation of the PFK2 gene, which encodes the beta-subunit of 6-phosphofructo-1-kinase, restores glycogen accumulation in snf1 cells...
  7. Boles E, Heinisch J, Zimmermann F. Different signals control the activation of glycolysis in the yeast Saccharomyces cerevisiae. Yeast. 1993;9:761-70 pubmed
    ..On the other hand, induction of pyruvate decarboxylase depends mainly on a signal in the lower part of glycolysis. ..
  8. Hofmann E, Eschrich K, Schellenberger W. Temporal organization of the phosphofructokinase/fructose-1,6-biphosphatase cycle. Adv Enzyme Regul. 1985;23:331-62 pubmed
    ..Generally, the glycolytic mode is favored by this effector because of its stimulatory action on the phosphofructokinase activity. ..