PEX5

Summary

Gene Symbol: PEX5
Description: Pex5p
Alias: PAS10, Pex5p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Platta H, El Magraoui F, Schlee D, Grunau S, Girzalsky W, Erdmann R. Ubiquitination of the peroxisomal import receptor Pex5p is required for its recycling. J Cell Biol. 2007;177:197-204 pubmed
    b>Pex5p, which is the import receptor for peroxisomal matrix proteins harboring a type I signal sequence (PTS1), is mono- and polyubiquitinated in Saccharomyces cerevisiae...
  2. Kiel J, Emmrich K, Meyer H, Kunau W. Ubiquitination of the peroxisomal targeting signal type 1 receptor, Pex5p, suggests the presence of a quality control mechanism during peroxisomal matrix protein import. J Biol Chem. 2005;280:1921-30 pubmed
    PEX genes encode proteins (peroxins) that are required for the biogenesis of peroxisomes. One of these peroxins, Pex5p, is the receptor for matrix proteins with a type 1 peroxisomal targeting signal (PTS1), which shuttles newly ..
  3. Rehling P, Marzioch M, Niesen F, Wittke E, Veenhuis M, Kunau W. The import receptor for the peroxisomal targeting signal 2 (PTS2) in Saccharomyces cerevisiae is encoded by the PAS7 gene. EMBO J. 1996;15:2901-13 pubmed
  4. Erdmann R, Blobel G. Identification of Pex13p a peroxisomal membrane receptor for the PTS1 recognition factor. J Cell Biol. 1996;135:111-21 pubmed
    ..Pex13p's most striking feature is an src homology 3 (SH3) domain that interacts directly with yeast Pex5p (former Pas10p), the recognition factor for the COOH-terminal tripeptide signal sequence (PTS1), but not with ..
  5. Williams C, van den Berg M, Sprenger R, Distel B. A conserved cysteine is essential for Pex4p-dependent ubiquitination of the peroxisomal import receptor Pex5p. J Biol Chem. 2007;282:22534-43 pubmed
    The peroxisomal protein import receptor Pex5p is modified by ubiquitin, both in an Ubc4p-dependent and -independent manner...
  6. Girzalsky W, Hoffmann L, Schemenewitz A, Nolte A, Kunau W, Erdmann R. Pex19p-dependent targeting of Pex17p, a peripheral component of the peroxisomal protein import machinery. J Biol Chem. 2006;281:19417-25 pubmed
  7. Williams C, van den Berg M, Panjikar S, Stanley W, Distel B, Wilmanns M. Insights into ubiquitin-conjugating enzyme/ co-activator interactions from the structure of the Pex4p:Pex22p complex. EMBO J. 2012;31:391-402 pubmed publisher
    ..in vitro, and Pex22p binding-deficient forms of Pex4p are unable to ubiquitinate the peroxisomal import receptor Pex5p in vivo...
  8. Meinecke M, Cizmowski C, Schliebs W, Krüger V, Beck S, Wagner R, et al. The peroxisomal importomer constitutes a large and highly dynamic pore. Nat Cell Biol. 2010;12:273-7 pubmed publisher
    ..Remarkably, the cycling import receptor Pex5p changes its topology from a soluble cytosolic form to an integral membrane-bound form...
  9. Albertini M, Rehling P, Erdmann R, Girzalsky W, Kiel J, Veenhuis M, et al. Pex14p, a peroxisomal membrane protein binding both receptors of the two PTS-dependent import pathways. Cell. 1997;89:83-92 pubmed
    ..The data presented here are consistent with the idea of a common translocation machinery for both PTS-dependent protein import pathways in the peroxisomal membrane...

More Information

Publications50

  1. Huhse B, Rehling P, Albertini M, Blank L, Meller K, Kunau W. Pex17p of Saccharomyces cerevisiae is a novel peroxin and component of the peroxisomal protein translocation machinery. J Cell Biol. 1998;140:49-60 pubmed
    ..point of convergence for the two peroxisomal targeting signal (PTS)-dependent import pathways, and indirectly to Pex5p, the PTS1 receptor...
  2. Girzalsky W, Rehling P, Stein K, Kipper J, Blank L, Kunau W, et al. Involvement of Pex13p in Pex14p localization and peroxisomal targeting signal 2-dependent protein import into peroxisomes. J Cell Biol. 1999;144:1151-62 pubmed
    ..Like Pex14p, Pex13p not only interacts with the PTS1-receptor Pex5p, but also with the PTS2-receptor Pex7p; however, this association may be direct or indirect...
  3. Kerssen D, Hambruch E, Klaas W, Platta H, de Kruijff B, Erdmann R, et al. Membrane association of the cycling peroxisome import receptor Pex5p. J Biol Chem. 2006;281:27003-15 pubmed
    ..carrying a peroxisome targeting signal type 1 (PTS1) are recognized in the cytosol by the cycling import receptor Pex5p. The receptor-cargo complex docks at the peroxisomal membrane where it associates with multimeric protein ..
  4. Platta H, El Magraoui F, Bäumer B, Schlee D, Girzalsky W, Erdmann R. Pex2 and pex12 function as protein-ubiquitin ligases in peroxisomal protein import. Mol Cell Biol. 2009;29:5505-16 pubmed publisher
    The PTS1-dependent peroxisomal matrix protein import is facilitated by the receptor protein Pex5 and can be divided into cargo recognition in the cytosol, membrane docking of the cargo-receptor complex, cargo release, and recycling of the ..
  5. Schafer A, Kerssen D, Veenhuis M, Kunau W, Schliebs W. Functional similarity between the peroxisomal PTS2 receptor binding protein Pex18p and the N-terminal half of the PTS1 receptor Pex5p. Mol Cell Biol. 2004;24:8895-906 pubmed
    Within the extended receptor cycle of peroxisomal matrix import, the function of the import receptor Pex5p comprises cargo recognition and transport...
  6. Hensel A, Beck S, El Magraoui F, Platta H, Girzalsky W, Erdmann R. Cysteine-dependent ubiquitination of Pex18p is linked to cargo translocation across the peroxisomal membrane. J Biol Chem. 2011;286:43495-505 pubmed publisher
    ..This finding indicates that Pex18p export is linked to cargo translocation, which supports the idea of an export-driven import of proteins into peroxisomes. ..
  7. Brocard C, Lametschwandtner G, Koudelka R, Hartig A. Pex14p is a member of the protein linkage map of Pex5p. EMBO J. 1997;16:5491-500 pubmed
    ..for peroxisomal proteins, we made use of the two-hybrid system to establish a protein linkage map centered around Pex5p from Saccharomyces cerevisiae, the receptor for the C-terminal peroxisomal targeting signal (PTS1)...
  8. Platta H, Grunau S, Rosenkranz K, Girzalsky W, Erdmann R. Functional role of the AAA peroxins in dislocation of the cycling PTS1 receptor back to the cytosol. Nat Cell Biol. 2005;7:817-22 pubmed
    ..2). One possible target for Pex4p is the PTS1 receptor Pex5p, which has recently been shown to be ubiquitinated...
  9. Williams C, van den Berg M, Geers E, Distel B. Pex10p functions as an E3 ligase for the Ubc4p-dependent ubiquitination of Pex5p. Biochem Biophys Res Commun. 2008;374:620-4 pubmed publisher
    The Saccharomyces cerevisiae (Sc) PTS1 import receptor Pex5p is modified by ubiquitin, both in an Ubc4p-dependent and a Pex4p (Ubc10p)-dependent manner...
  10. Elgersma Y, Kwast L, Klein A, Voorn Brouwer T, Van den Berg M, Metzig B, et al. The SH3 domain of the Saccharomyces cerevisiae peroxisomal membrane protein Pex13p functions as a docking site for Pex5p, a mobile receptor for the import PTS1-containing proteins. J Cell Biol. 1996;135:97-109 pubmed
    ..A two-hybrid screen with the SH3 domain of Pex13p identified Pex5p, a receptor for proteins with a type I peroxisomal targeting signal (PTS1), as its ligand...
  11. Debelyy M, Platta H, Saffian D, Hensel A, Thoms S, Meyer H, et al. Ubp15p, a ubiquitin hydrolase associated with the peroxisomal export machinery. J Biol Chem. 2011;286:28223-34 pubmed publisher
    ..and is capable of cleaving off ubiquitin moieties from the type I peroxisomal targeting sequence (PTS1) receptor Pex5p. Furthermore, Ubp15p-deficient cells are characterized by a stress-related PTS1 import defect...
  12. Kragt A, Voorn Brouwer T, van den Berg M, Distel B. The Saccharomyces cerevisiae peroxisomal import receptor Pex5p is monoubiquitinated in wild type cells. J Biol Chem. 2005;280:7867-74 pubmed
    b>Pex5p is a mobile receptor for peroxisomal targeting signal type I-containing proteins that cycles between the cytoplasm and the peroxisome. Here we show that Pex5p is a stable protein that is monoubiquitinated in wild type cells...
  13. Schell Steven A, Stein K, Amoros M, Landgraf C, Volkmer Engert R, Rottensteiner H, et al. Identification of a novel, intraperoxisomal pex14-binding site in pex13: association of pex13 with the docking complex is essential for peroxisomal matrix protein import. Mol Cell Biol. 2005;25:3007-18 pubmed
    ..The core protein of this complex, Pex14, is thought to represent the initial docking site for the import receptors Pex5 and Pex7. Associated with this complex is a fraction of Pex13, another essential component of the import machinery...
  14. Elgersma Y, van Roermund C, Wanders R, Tabak H. Peroxisomal and mitochondrial carnitine acetyltransferases of Saccharomyces cerevisiae are encoded by a single gene. EMBO J. 1995;14:3472-9 pubmed
    ..Import of CAT via this internal signal was shown to be dependent on PAS10, a protein which is required for the import of PTS-1 containing proteins...
  15. Effelsberg D, Cruz Zaragoza L, Schliebs W, Erdmann R. Pex9p is a new yeast peroxisomal import receptor for PTS1-containing proteins. J Cell Sci. 2016;129:4057-4066 pubmed
    ..carrying a type 1 peroxisomal targeting signal (PTS1) are recognized by the well-conserved cycling import receptor Pex5p. The yeast YMR018W gene encodes a Pex5p paralog and newly identified peroxin that is involved in peroxisomal ..
  16. Agne B, Meindl N, Niederhoff K, Einwächter H, Rehling P, Sickmann A, et al. Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. Mol Cell. 2003;11:635-46 pubmed
    Peroxisomes transport folded and oligomeric proteins across their membrane. Two cytosolic import receptors, Pex5p and Pex7p, along with approximately 12 membrane-bound peroxins participate in this process...
  17. Deckers M, Emmrich K, Girzalsky W, Awa W, Kunau W, Erdmann R. Targeting of Pex8p to the peroxisomal importomer. Eur J Cell Biol. 2010;89:924-31 pubmed publisher
  18. Klein A, Barnett P, Bottger G, Konings D, Tabak H, Distel B. Recognition of peroxisomal targeting signal type 1 by the import receptor Pex5p. J Biol Chem. 2001;276:15034-41 pubmed
    We have studied how Pex5p recognizes peroxisomal targeting signal type 1 (PTS1)-containing proteins...
  19. Grou C, Carvalho A, Pinto M, Wiese S, Piechura H, Meyer H, et al. Members of the E2D (UbcH5) family mediate the ubiquitination of the conserved cysteine of Pex5p, the peroxisomal import receptor. J Biol Chem. 2008;283:14190-7 pubmed publisher
    ..of peroxisomal biogenesis, newly synthesized peroxisomal matrix proteins are transported into the organelle by Pex5p. Pex5p recognizes these proteins in the cytosol, mediates their membrane translocation, and is exported back into ..
  20. Pires J, Hong X, Brockmann C, Volkmer Engert R, Schneider Mergener J, Oschkinat H, et al. The ScPex13p SH3 domain exposes two distinct binding sites for Pex5p and Pex14p. J Mol Biol. 2003;326:1427-35 pubmed
    ..machinery and interacts via its C-terminal SH3 domain with the type II SH3-ligand Pex14p and the non-PXXP protein Pex5p. We report the solution structure of the SH3 domain of Pex13p from Saccharomyces cerevisiae and the identification ..
  21. El Magraoui F, Schrötter A, Brinkmeier R, Kunst L, Mastalski T, Müller T, et al. The cytosolic domain of Pex22p stimulates the Pex4p-dependent ubiquitination of the PTS1-receptor. PLoS ONE. 2014;9:e105894 pubmed publisher
    ..A key step is the monoubiquitination of the import receptor Pex5p by the ubiquitin-conjugating enzyme (E2) Pex4p...
  22. Barnett P, Bottger G, Klein A, Tabak H, Distel B. The peroxisomal membrane protein Pex13p shows a novel mode of SH3 interaction. EMBO J. 2000;19:6382-91 pubmed
    ..the SH3 domain of the integral peroxisomal membrane protein Pex13p is able to bind two proteins, one of which, Pex5p, represents a novel non-P-X-X-P ligand...
  23. Grimm I, Saffian D, Girzalsky W, Erdmann R. Nucleotide-dependent assembly of the peroxisomal receptor export complex. Sci Rep. 2016;6:19838 pubmed publisher
    ..At the peroxisomal membrane, the complex is responsible for the release of the import receptor Pex5p at the end of the matrix protein import cycle...
  24. Grunau S, Schliebs W, Linnepe R, Neufeld C, Cizmowski C, Reinartz B, et al. Peroxisomal targeting of PTS2 pre-import complexes in the yeast Saccharomyces cerevisiae. Traffic. 2009;10:451-60 pubmed publisher
    ..Unlike the PTS1 receptor Pex5p, the PTS2 receptor Pex7p is necessary but not sufficient to target cargo proteins into the peroxisomal matrix and ..
  25. Oeljeklaus S, Reinartz B, Wolf J, Wiese S, Tonillo J, Podwojski K, et al. Identification of core components and transient interactors of the peroxisomal importomer by dual-track stable isotope labeling with amino acids in cell culture analysis. J Proteome Res. 2012;11:2567-80 pubmed publisher
    ..virtually all known Pex14p interaction partners including the core constituents of the importomer as well as Pex5p, Pex11p, Pex15p, and Dyn2p...
  26. Hagen S, Drepper F, Fischer S, Fodor K, Passon D, Platta H, et al. Structural insights into cargo recognition by the yeast PTS1 receptor. J Biol Chem. 2015;290:26610-26 pubmed publisher
    ..The import receptor Pex5p mediates the import of proteins harboring a peroxisomal targeting signal of type I (PTS1)...
  27. Gardner B, Castanzo D, Chowdhury S, Stjepanovic G, Stefely M, Hurley J, et al. The peroxisomal AAA-ATPase Pex1/Pex6 unfolds substrates by processive threading. Nat Commun. 2018;9:135 pubmed publisher
    ..Furthermore, Pex15 directly binds the cargo receptor Pex5, linking Pex1/Pex6 to other components of the peroxisomal import machinery...
  28. Rosenkranz K, Birschmann I, Grunau S, Girzalsky W, Kunau W, Erdmann R. Functional association of the AAA complex and the peroxisomal importomer. FEBS J. 2006;273:3804-15 pubmed
    ..are components of the peroxisomal protein import machinery required for the relocation of the import receptor Pex5p from the peroxisomal membrane to the cytosol...
  29. Klein A, van den Berg M, Bottger G, Tabak H, Distel B. Saccharomyces cerevisiae acyl-CoA oxidase follows a novel, non-PTS1, import pathway into peroxisomes that is dependent on Pex5p. J Biol Chem. 2002;277:25011-9 pubmed
    ..Here we demonstrate that peroxisomal import of Pox1p is nevertheless dependent on binding to Pex5p, the PTS1 import receptor...
  30. Yang X, Purdue P, Lazarow P. Eci1p uses a PTS1 to enter peroxisomes: either its own or that of a partner, Dci1p. Eur J Cell Biol. 2001;80:126-38 pubmed
    ..protein (GFP) is not dependent on Pex7p (the PTS2 receptor), ruling out a PTS2 mechanism, but is dependent on Pex5p (the PTS1 receptor)...
  31. Bottger G, Barnett P, Klein A, Kragt A, Tabak H, Distel B. Saccharomyces cerevisiae PTS1 receptor Pex5p interacts with the SH3 domain of the peroxisomal membrane protein Pex13p in an unconventional, non-PXXP-related manner. Mol Biol Cell. 2000;11:3963-76 pubmed
    ..of proteins into peroxisomes, among which is the receptor for peroxisomal targeting signal 1 (PTS1) proteins Pex5p, the integral membrane protein Pex13p, which contains an Src homology 3 (SH3) domain, and the peripheral membrane ..
  32. Brocard C, Kragler F, Simon M, Schuster T, Hartig A. The tetratricopeptide repeat-domain of the PAS10 protein of Saccharomyces cerevisiae is essential for binding the peroxisomal targeting signal-SKL. Biochem Biophys Res Commun. 1994;204:1016-22 pubmed
    The PAS10 gene was found in a two-hybrid screen for the isolation of genes encoding proteins which interact with the C-terminal peroxisomal targeting signal -SKL...
  33. Williams C, van den Berg M, Distel B. Saccharomyces cerevisiae Pex14p contains two independent Pex5p binding sites, which are both essential for PTS1 protein import. FEBS Lett. 2005;579:3416-20 pubmed
    Pex14p is a peroxisomal membrane-associated protein involved in docking of both Pex5p and Pex7p to the peroxisomal membrane...
  34. Albertini M, Girzalsky W, Veenhuis M, Kunau W. Pex12p of Saccharomyces cerevisiae is a component of a multi-protein complex essential for peroxisomal matrix protein import. Eur J Cell Biol. 2001;80:257-70 pubmed
    ..In immunoisolation experiments Pex12p was co-purified with the RING finger protein Pex10p, the PTS1 receptor Pex5p and the docking proteins for the PTS1 and the PTS2 receptor at the peroxisomal membrane, Pex13p and Pex14p...
  35. Rehling P, Skaletz Rorowski A, Girzalsky W, Voorn Brouwer T, Franse M, Distel B, et al. Pex8p, an intraperoxisomal peroxin of Saccharomyces cerevisiae required for protein transport into peroxisomes binds the PTS1 receptor pex5p. J Biol Chem. 2000;275:3593-602 pubmed
    ..localization, Pex8p contains a peroxisomal targeting signal 1, and it interacts with the PTS1 receptor Pex5p. However, the Pex5p/Pex8p association is also observed upon deletion of the PTS1 of Pex8p, suggesting that Pex8p ..
  36. Niederhoff K, Meindl Beinker N, Kerssen D, Perband U, Schafer A, Schliebs W, et al. Yeast Pex14p possesses two functionally distinct Pex5p and one Pex7p binding sites. J Biol Chem. 2005;280:35571-8 pubmed
    ..the docking subcomplex at the peroxisomal membrane and interact in this cycle with both soluble import receptors Pex5p and Pex7p...
  37. Eckert J, Johnsson N. Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome. J Cell Sci. 2003;116:3623-34 pubmed
    ..A yeast strain harboring a deletion of PEX3 enabled us to estimate the influence of the peroxisomal membrane on the formation of a subset of the investigated protein-protein interactions. ..
  38. Douangamath A, Filipp F, Klein A, Barnett P, Zou P, Voorn Brouwer T, et al. Topography for independent binding of alpha-helical and PPII-helical ligands to a peroxisomal SH3 domain. Mol Cell. 2002;10:1007-17 pubmed
    ..interactions, the peroxisomal Pex13p SH3 domain has the unique capacity of binding to two different ligands, Pex5p and Pex14p. We have used this domain as a model to decipher its structurally independent ligand binding sites...
  39. Schwartzkopff B, Platta H, Hasan S, Girzalsky W, Erdmann R. Cysteine-specific ubiquitination protects the peroxisomal import receptor Pex5p against proteasomal degradation. Biosci Rep. 2015;35: pubmed publisher
    ..Proteins with a type 1 peroxisomal targeting signal (PTS1) are bound by the import receptor Pex5p in the cytosol and guided to the peroxisomal membrane...
  40. Chan A, Schummer A, Fischer S, Schröter T, Cruz Zaragoza L, Bender J, et al. Pex17p-dependent assembly of Pex14p/Dyn2p-subcomplexes of the peroxisomal protein import machinery. Eur J Cell Biol. 2016;95:585-597 pubmed publisher
    ..As dyn2? cells also display reduced peroxisomal function, our data indicate that Dyn2p-dependent formation of the high molecular weight Pex14p-complex is required to maintain peroxisomal function on wild-type level. ..
  41. Wróblewska J, Cruz Zaragoza L, Yuan W, Schummer A, Chuartzman S, de Boer R, et al. Saccharomyces cerevisiae cells lacking Pex3 contain membrane vesicles that harbor a subset of peroxisomal membrane proteins. Biochim Biophys Acta Mol Cell Res. 2017;1864:1656-1667 pubmed publisher
    ..At the vesicles, Pex14 assembles with other peroxins (Pex13, Pex17, and Pex5) to form a complex with a composition similar to the PTS1 import pore in wild-type cells...