Gene Symbol: PEX4
Description: E2 ubiquitin-protein ligase peroxin 4
Alias: PAS2, UBC10, E2 ubiquitin-protein ligase peroxin 4
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. El Magraoui F, Brinkmeier R, Schrötter A, Girzalsky W, Müller T, Marcus K, et al. Distinct ubiquitination cascades act on the peroxisomal targeting signal type 2 co-receptor Pex18p. Traffic. 2013;14:1290-301 pubmed publisher
    ..Our findings for the PTS2-pathway complement the data on PTS1-receptor ubiquitination and add up to a unified concept of the ubiquitin-based regulation of peroxisomal import. ..
  2. El Magraoui F, Bäumer B, Platta H, Baumann J, Girzalsky W, Erdmann R. The RING-type ubiquitin ligases Pex2p, Pex10p and Pex12p form a heteromeric complex that displays enhanced activity in an ubiquitin conjugating enzyme-selective manner. FEBS J. 2012;279:2060-70 pubmed publisher
    ..The RING domains proved to function as heteromeric pairs that display an Pex10p-dependent enhanced ligase activity in an ubiquitin conjugating enzyme-selective manner. ..
  3. Platta H, El Magraoui F, Schlee D, Grunau S, Girzalsky W, Erdmann R. Ubiquitination of the peroxisomal import receptor Pex5p is required for its recycling. J Cell Biol. 2007;177:197-204 pubmed
    ..Therefore, the energy requirement of the peroxisomal import pathway has to be extended by a second ATP-dependent step, namely receptor monoubiquitination. ..
  4. Williams C, van den Berg M, Sprenger R, Distel B. A conserved cysteine is essential for Pex4p-dependent ubiquitination of the peroxisomal import receptor Pex5p. J Biol Chem. 2007;282:22534-43 pubmed
    ..Together, our results strongly suggest that Pex4p-dependent ubiquitination of Pex5p occurs on a cysteine residue. ..
  5. Wiebel F, Kunau W. The Pas2 protein essential for peroxisome biogenesis is related to ubiquitin-conjugating enzymes. Nature. 1992;359:73-6 pubmed
    ..The proposed function of the Pas2 protein as a UBC enzyme (UBC10) is supported by the fact that site-directed mutagenesis of a strictly conserved and functionally essential ..
  6. Platta H, El Magraoui F, Bäumer B, Schlee D, Girzalsky W, Erdmann R. Pex2 and pex12 function as protein-ubiquitin ligases in peroxisomal protein import. Mol Cell Biol. 2009;29:5505-16 pubmed publisher
    ..Recently, the ubiquitin-conjugating enzymes involved in Pex5 ubiquitination were identified as Ubc4 and Pex4 (Ubc10), whereas the identity of the corresponding protein-ubiquitin ligases remained unknown...
  7. Erdmann R, Veenhuis M, Mertens D, Kunau W. Isolation of peroxisome-deficient mutants of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1989;86:5419-23 pubmed
    ..These lesions (pas1-1 and pas2) are shown to be nonallelic and recessive...
  8. Williams C, van den Berg M, Stanley W, Wilmanns M, Distel B. A disulphide bond in the E2 enzyme Pex4p modulates ubiquitin-conjugating activity. Sci Rep. 2013;3:2212 pubmed publisher
    ..Finally, based on sequence and structural alignments, we have identified other ubiquitin-conjugating enzymes that may contain disulphide bonds. ..
  9. Williams C, van den Berg M, Panjikar S, Stanley W, Distel B, Wilmanns M. Insights into ubiquitin-conjugating enzyme/ co-activator interactions from the structure of the Pex4p:Pex22p complex. EMBO J. 2012;31:391-402 pubmed publisher
    ..Our data demonstrate that the Pex4p:Pex22p assembly, and not Pex4p alone, functions as the E2 enzyme required for Pex5p ubiquitination, establishing a novel mechanism of E2 enzyme regulation. ..

More Information


  1. Halbach A, Rucktäschel R, Rottensteiner H, Erdmann R. The N-domain of Pex22p can functionally replace the Pex3p N-domain in targeting and peroxisome formation. J Biol Chem. 2009;284:3906-16 pubmed publisher
    ..The C-terminal domain of Pex3p thus contains the relevant information required for de novo peroxisome formation, thereby challenging the concept of the N terminus of Pex3p being key in that process. ..
  2. Williams C, van den Berg M, Geers E, Distel B. Pex10p functions as an E3 ligase for the Ubc4p-dependent ubiquitination of Pex5p. Biochem Biophys Res Commun. 2008;374:620-4 pubmed publisher
    ..These data imply that the two distinct Pex5p modifications require different E(3) ligases, as well as different E(2) enzymes. ..
  3. Eckert J, Johnsson N. Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome. J Cell Sci. 2003;116:3623-34 pubmed
    ..A yeast strain harboring a deletion of PEX3 enabled us to estimate the influence of the peroxisomal membrane on the formation of a subset of the investigated protein-protein interactions. ..
  4. Johnson E, Blobel G. Ubc9p is the conjugating enzyme for the ubiquitin-like protein Smt3p. J Biol Chem. 1997;272:26799-802 pubmed
    ..cerevisiae. These results suggest that, like ubiquitination, Smt3p conjugation may be a critical modification in cell cycle regulation. ..
  5. Hochstrasser M. Ubiquitin-dependent protein degradation. Annu Rev Genet. 1996;30:405-39 pubmed
    ..This diversity underlies both the high substrate specificity of the ubiquitin system and the variety of regulatory mechanisms that it serves. ..
  6. El Magraoui F, Schrötter A, Brinkmeier R, Kunst L, Mastalski T, Müller T, et al. The cytosolic domain of Pex22p stimulates the Pex4p-dependent ubiquitination of the PTS1-receptor. PLoS ONE. 2014;9:e105894 pubmed publisher
    ..However, this Pex3(N)-Pex4p chimera can only complement the double-deletion strain pex4Δ/pex22Δ and ensure optimal Pex5p-ubiquitination when the C-terminal part of Pex22p is additionally ..
  7. Hiraishi H, Mochizuki M, Takagi H. Enhancement of stress tolerance in Saccharomyces cerevisiae by overexpression of ubiquitin ligase Rsp5 and ubiquitin-conjugating enzymes. Biosci Biotechnol Biochem. 2006;70:2762-5 pubmed
    ..Co-overexpression of Rsp5 and Ubc1, Ubc2, Ubc3, Ubc5, Ubc6, Ubc9, Ubc10, Ubc11, Ubc12, or Ubc13 further enhanced stress tolerance...
  8. Wróblewska J, Cruz Zaragoza L, Yuan W, Schummer A, Chuartzman S, de Boer R, et al. Saccharomyces cerevisiae cells lacking Pex3 contain membrane vesicles that harbor a subset of peroxisomal membrane proteins. Biochim Biophys Acta Mol Cell Res. 2017;1864:1656-1667 pubmed publisher
    ..revealed that also the PTS2 receptor Pex7, the importomer organizing peroxin Pex8, the ubiquitin conjugating enzyme Pex4 with its recruiting PMP Pex22, as well as Pex15 and Pex25 co-localize with Pex14...