PEX3

Summary

Gene Symbol: PEX3
Description: Pex3p
Alias: PAS3, Pex3p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Girzalsky W, Rehling P, Stein K, Kipper J, Blank L, Kunau W, et al. Involvement of Pex13p in Pex14p localization and peroxisomal targeting signal 2-dependent protein import into peroxisomes. J Cell Biol. 1999;144:1151-62 pubmed
    ..We also found Pex13p to be required for peroxisomal association of Pex14p, yet the SH3 domain of Pex13p may not provide the only binding site for Pex14p at the peroxisomal membrane...
  2. Hoepfner D, Schildknegt D, Braakman I, Philippsen P, Tabak H. Contribution of the endoplasmic reticulum to peroxisome formation. Cell. 2005;122:85-95 pubmed
    ..Both human and yeast cells lacking peroxisomes due to mutations in PEX3 or PEX19 genes regenerate the organelles upon reintroduction of the corresponding wild-type version...
  3. Tam Y, Fagarasanu A, Fagarasanu M, Rachubinski R. Pex3p initiates the formation of a preperoxisomal compartment from a subdomain of the endoplasmic reticulum in Saccharomyces cerevisiae. J Biol Chem. 2005;280:34933-9 pubmed
    ..for de novo peroxisome synthesis comes from studies of cells lacking the peroxisomal integral membrane peroxin Pex3p. These cells lack peroxisomes, but peroxisomes can assemble upon reintroduction of Pex3p...
  4. Motley A, Hettema E. Yeast peroxisomes multiply by growth and division. J Cell Biol. 2007;178:399-410 pubmed
    ..A marker for the maturation pathway, Pex3-GFP, is delivered from the ER to existing peroxisomes...
  5. Chang J, Mast F, Fagarasanu A, Rachubinski D, Eitzen G, Dacks J, et al. Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors. J Cell Biol. 2009;187:233-46 pubmed publisher
    ..Here, we demonstrate an unexpected role for Pex3 proteins in peroxisome inheritance. Both Pex3p and Pex3Bp are peroxisomal integral membrane proteins that function as peroxisomal receptors for class V myosin ..
  6. Rucktäschel R, Halbach A, Girzalsky W, Rottensteiner H, Erdmann R. De novo synthesis of peroxisomes upon mitochondrial targeting of Pex3p. Eur J Cell Biol. 2010;89:947-54 pubmed publisher
    ..b>Pex3p is the key component for both pathways and its targeting to the ER is thought to initiate the de novo formation of ..
  7. Hohfeld J, Veenhuis M, Kunau W. PAS3, a Saccharomyces cerevisiae gene encoding a peroxisomal integral membrane protein essential for peroxisome biogenesis. J Cell Biol. 1991;114:1167-78 pubmed
    Saccharomyces cerevisiae pas3-mutants are described which conform the pas-phenotype recently reported for the peroxisomal assembly mutants pas1-1 and pas2 (Erdmann, R., M. Veenhuis, D. Mertens, and W.-H Kunau, 1989, Proc. Natl. Acad. Sci...
  8. Hettema E, Girzalsky W, Van den Berg M, Erdmann R, Distel B. Saccharomyces cerevisiae pex3p and pex19p are required for proper localization and stability of peroxisomal membrane proteins. EMBO J. 2000;19:223-33 pubmed
    ..Even Pex15p requires Pex3p and Pex19p for localization to peroxisomal membranes...
  9. Munck J, Motley A, Nuttall J, Hettema E. A dual function for Pex3p in peroxisome formation and inheritance. J Cell Biol. 2009;187:463-71 pubmed publisher
    Saccharomyces cerevisiae Pex3p has been shown to act at the ER during de novo peroxisome formation. However, its steady state is at the peroxisomal membrane, where its role is debated...

More Information

Publications23

  1. Weir N, Kamber R, Martenson J, Denic V. The AAA protein Msp1 mediates clearance of excess tail-anchored proteins from the peroxisomal membrane. elife. 2017;6: pubmed publisher
    ..Lastly, we show that Pex15 interacts with the peroxisomal membrane protein Pex3, which shields Pex15 from Msp1-dependent turnover...
  2. Knoblach B, Sun X, Coquelle N, Fagarasanu A, Poirier R, Rachubinski R. An ER-peroxisome tether exerts peroxisome population control in yeast. EMBO J. 2013;32:2439-53 pubmed publisher
    ..The tether consists of the peroxisome biogenic protein, Pex3p, and the peroxisome inheritance factor, Inp1p...
  3. Lefevre S, Van Roermund C, Wanders R, Veenhuis M, van der Klei I. The significance of peroxisome function in chronological aging of Saccharomyces cerevisiae. Aging Cell. 2013;12:784-93 pubmed publisher
    ..We analyzed four different peroxisome-deficient (pex) phenotypes. These included ?pex3 cells that lack peroxisomal membranes and in which all peroxisomal proteins are mislocalized together with ?pex6 in ..
  4. Motley A, Nuttall J, Hettema E. Pex3-anchored Atg36 tags peroxisomes for degradation in Saccharomyces cerevisiae. EMBO J. 2012;31:2852-68 pubmed publisher
    ..b>Pex3 is central to the formation of peroxisomes and their segregation because it recruits factors specific for these ..
  5. Lam S, Yoda N, Schekman R. A vesicle carrier that mediates peroxisome protein traffic from the endoplasmic reticulum. Proc Natl Acad Sci U S A. 2010;107:21523-8 pubmed publisher
    Pex19p, a soluble cytoplasmic transport protein, is required for the traffic of the peroxisomal membrane proteins Pex3p and Pex15p from the endoplasmic reticulum (ER) to the peroxisome...
  6. Rucktäschel R, Thoms S, Sidorovitch V, Halbach A, Pechlivanis M, Volkmer R, et al. Farnesylation of pex19p is required for its structural integrity and function in peroxisome biogenesis. J Biol Chem. 2009;284:20885-96 pubmed publisher
    ..in vivo and that this modification is independent of peroxisome induction or the Pex19p membrane anchor Pex3p. Furthermore, genomic mutations of PEX19 prove that farnesylation is essential for proper matrix protein import ..
  7. Halbach A, Rucktäschel R, Rottensteiner H, Erdmann R. The N-domain of Pex22p can functionally replace the Pex3p N-domain in targeting and peroxisome formation. J Biol Chem. 2009;284:3906-16 pubmed publisher
    b>Pex3p is a central component of the import machinery for peroxisomal membrane proteins (PMPs) that can reach peroxisomes via the endoplasmic reticulum (ER) and even trigger de novo peroxisome formation from the ER...
  8. Kerssen D, Hambruch E, Klaas W, Platta H, de Kruijff B, Erdmann R, et al. Membrane association of the cycling peroxisome import receptor Pex5p. J Biol Chem. 2006;281:27003-15 pubmed
    ..Altogether, these data strongly suggest that a translocation-competent state of the PTS1 receptor enters the membrane via protein-lipid interactions before it tightly associates with other peroxins. ..
  9. van der Zand A, Braakman I, Tabak H. Peroxisomal membrane proteins insert into the endoplasmic reticulum. Mol Biol Cell. 2010;21:2057-65 pubmed publisher
    ..PMPs leave the ER in a Pex3p-Pex19p-dependent manner to end up in metabolically active peroxisomes...
  10. Eckert J, Johnsson N. Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome. J Cell Sci. 2003;116:3623-34 pubmed
    ..A yeast strain harboring a deletion of PEX3 enabled us to estimate the influence of the peroxisomal membrane on the formation of a subset of the investigated ..
  11. Wang S, Horn P, Liou L, Muggeridge M, Zhang Z, Chapman K, et al. A peroxisome biogenesis deficiency prevents the binding of alpha-synuclein to lipid droplets in lipid-loaded yeast. Biochem Biophys Res Commun. 2013;438:452-6 pubmed publisher
    ..in lipid-loaded, wild-type yeast cells but not to lipid droplets in lipid-loaded, peroxisome-deficient cells (pex3?)...
  12. Tanaka C, Tan L, Mochida K, Kirisako H, Koizumi M, Asai E, et al. Hrr25 triggers selective autophagy-related pathways by phosphorylating receptor proteins. J Cell Biol. 2014;207:91-105 pubmed publisher
  13. Wróblewska J, Cruz Zaragoza L, Yuan W, Schummer A, Chuartzman S, de Boer R, et al. Saccharomyces cerevisiae cells lacking Pex3 contain membrane vesicles that harbor a subset of peroxisomal membrane proteins. Biochim Biophys Acta Mol Cell Res. 2017;1864:1656-1667 pubmed publisher
    b>Pex3 has been proposed to be important for the exit of peroxisomal membrane proteins (PMPs) from the ER, based on the observation that PMPs accumulate at the ER in Saccharomyces cerevisiae pex3 mutant cells...
  14. Gotte K, Girzalsky W, Linkert M, Baumgart E, Kammerer S, Kunau W, et al. Pex19p, a farnesylated protein essential for peroxisome biogenesis. Mol Cell Biol. 1998;18:616-28 pubmed
    ..Farnesylation of Pex19p was shown to be essential for the proper function of the protein in peroxisome biogenesis. Pex19p was shown to interact with Pex3p in vivo, and this interaction required farnesylation of Pex19p.