Gene Symbol: Pex22p
Description: ubiquitin-protein transferase activating protein PEX22
Alias: YAF5, ubiquitin-protein transferase activating protein PEX22
Species: Saccharomyces cerevisiae S288c
Williams C, van den Berg M, Panjikar S, Stanley W, Distel B, Wilmanns M. Insights into ubiquitin-conjugating enzyme/ co-activator interactions from the structure of the Pex4p:Pex22p complex. EMBO J. 2012;31:391-402 pubmed publisher
..Here, we have determined the crystal structure and function of an E2/co-activator assembly, the Pex4p:Pex22p complex...
Platta H, El Magraoui F, Schlee D, Grunau S, Girzalsky W, Erdmann R. Ubiquitination of the peroxisomal import receptor Pex5p is required for its recycling. J Cell Biol. 2007;177:197-204 pubmed
..Therefore, the energy requirement of the peroxisomal import pathway has to be extended by a second ATP-dependent step, namely receptor monoubiquitination. ..
Williams C, van den Berg M, Stanley W, Wilmanns M, Distel B. A disulphide bond in the E2 enzyme Pex4p modulates ubiquitin-conjugating activity. Sci Rep. 2013;3:2212 pubmed publisher
The ubiquitin-conjugating enzyme Pex4p together with its binding partner, the peroxisomal membrane protein Pex22p, co-ordinates cysteine-dependent ubiquitination of the cycling receptor protein Pex5p...
Halbach A, Rucktäschel R, Rottensteiner H, Erdmann R. The N-domain of Pex22p can functionally replace the Pex3p N-domain in targeting and peroxisome formation. J Biol Chem. 2009;284:3906-16 pubmed publisher
..domain of Pex3p can be functionally replaced by the N-terminal peroxisomal membrane targeting signal (mPTS) of Pex22p, a supposedly unrelated component of the import machinery for peroxisomal matrix proteins...
Koller A, Snyder W, Faber K, Wenzel T, Rangell L, Keller G, et al
. Pex22p of Pichia pastoris, essential for peroxisomal matrix protein import, anchors the ubiquitin-conjugating enzyme, Pex4p, on the peroxisomal membrane. J Cell Biol. 1999;146:99-112 pubmed
..Cloning the gene by complementation revealed that the encoded protein, Pex22p, is a new peroxin...
El Magraoui F, SchrÃ¶tter A, Brinkmeier R, Kunst L, Mastalski T, MÃ¼ller T, et al
. The cytosolic domain of Pex22p stimulates the Pex4p-dependent ubiquitination of the PTS1-receptor. PLoS ONE. 2014;9:e105894 pubmed publisher
..Recently, it has been reported that the soluble Pex4p requires the interaction to its peroxisomal membrane-anchor Pex22p to display full activity...
Wróblewska J, Cruz Zaragoza L, Yuan W, Schummer A, Chuartzman S, de Boer R, et al
. Saccharomyces cerevisiae cells lacking Pex3 contain membrane vesicles that harbor a subset of peroxisomal membrane proteins. Biochim Biophys Acta Mol Cell Res. 2017;1864:1656-1667 pubmed publisher
..Our data suggest that peroxisomal membrane vesicles can form in the absence of Pex3 and that several PMPs can insert in these vesicles in a Pex3 independent manner. ..
Eckert J, Johnsson N. Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome. J Cell Sci. 2003;116:3623-34 pubmed
..A yeast strain harboring a deletion of PEX3 enabled us to estimate the influence of the peroxisomal membrane on the formation of a subset of the investigated protein-protein interactions. ..