Gene Symbol: Pex22p
Description: ubiquitin-protein transferase activating protein PEX22
Alias: YAF5, ubiquitin-protein transferase activating protein PEX22
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Williams C, van den Berg M, Panjikar S, Stanley W, Distel B, Wilmanns M. Insights into ubiquitin-conjugating enzyme/ co-activator interactions from the structure of the Pex4p:Pex22p complex. EMBO J. 2012;31:391-402 pubmed publisher
    ..Here, we have determined the crystal structure and function of an E2/co-activator assembly, the Pex4p:Pex22p complex...
  2. Platta H, El Magraoui F, Schlee D, Grunau S, Girzalsky W, Erdmann R. Ubiquitination of the peroxisomal import receptor Pex5p is required for its recycling. J Cell Biol. 2007;177:197-204 pubmed
    ..Therefore, the energy requirement of the peroxisomal import pathway has to be extended by a second ATP-dependent step, namely receptor monoubiquitination. ..
  3. Williams C, van den Berg M, Stanley W, Wilmanns M, Distel B. A disulphide bond in the E2 enzyme Pex4p modulates ubiquitin-conjugating activity. Sci Rep. 2013;3:2212 pubmed publisher
    The ubiquitin-conjugating enzyme Pex4p together with its binding partner, the peroxisomal membrane protein Pex22p, co-ordinates cysteine-dependent ubiquitination of the cycling receptor protein Pex5p...
  4. Halbach A, Rucktäschel R, Rottensteiner H, Erdmann R. The N-domain of Pex22p can functionally replace the Pex3p N-domain in targeting and peroxisome formation. J Biol Chem. 2009;284:3906-16 pubmed publisher
    ..domain of Pex3p can be functionally replaced by the N-terminal peroxisomal membrane targeting signal (mPTS) of Pex22p, a supposedly unrelated component of the import machinery for peroxisomal matrix proteins...
  5. Koller A, Snyder W, Faber K, Wenzel T, Rangell L, Keller G, et al. Pex22p of Pichia pastoris, essential for peroxisomal matrix protein import, anchors the ubiquitin-conjugating enzyme, Pex4p, on the peroxisomal membrane. J Cell Biol. 1999;146:99-112 pubmed
    ..Cloning the gene by complementation revealed that the encoded protein, Pex22p, is a new peroxin...
  6. El Magraoui F, Schrötter A, Brinkmeier R, Kunst L, Mastalski T, Müller T, et al. The cytosolic domain of Pex22p stimulates the Pex4p-dependent ubiquitination of the PTS1-receptor. PLoS ONE. 2014;9:e105894 pubmed publisher
    ..Recently, it has been reported that the soluble Pex4p requires the interaction to its peroxisomal membrane-anchor Pex22p to display full activity...
  7. Wróblewska J, Cruz Zaragoza L, Yuan W, Schummer A, Chuartzman S, de Boer R, et al. Saccharomyces cerevisiae cells lacking Pex3 contain membrane vesicles that harbor a subset of peroxisomal membrane proteins. Biochim Biophys Acta Mol Cell Res. 2017;1864:1656-1667 pubmed publisher
    ..Our data suggest that peroxisomal membrane vesicles can form in the absence of Pex3 and that several PMPs can insert in these vesicles in a Pex3 independent manner. ..
  8. Eckert J, Johnsson N. Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome. J Cell Sci. 2003;116:3623-34 pubmed
    ..A yeast strain harboring a deletion of PEX3 enabled us to estimate the influence of the peroxisomal membrane on the formation of a subset of the investigated protein-protein interactions. ..