Pex15p

Summary

Gene Symbol: Pex15p
Description: Pex15p
Alias: PAS21, Pex15p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Elgersma Y, Kwast L, Van den Berg M, Snyder W, Distel B, Subramani S, et al. Overexpression of Pex15p, a phosphorylated peroxisomal integral membrane protein required for peroxisome assembly in S.cerevisiae, causes proliferation of the endoplasmic reticulum membrane. EMBO J. 1997;16:7326-41 pubmed
    ..PEX15 encodes a phosphorylated, integral peroxisomal membrane protein (Pex15p)...
  2. Birschmann I, Stroobants A, van den Berg M, Schafer A, Rosenkranz K, Kunau W, et al. Pex15p of Saccharomyces cerevisiae provides a molecular basis for recruitment of the AAA peroxin Pex6p to peroxisomal membranes. Mol Biol Cell. 2003;14:2226-36 pubmed
    ..Here we show that Pex15p, an integral peroxisomal membrane protein, in vivo and in vitro binds the AAA peroxin Pex6p...
  3. Platta H, El Magraoui F, Bäumer B, Schlee D, Girzalsky W, Erdmann R. Pex2 and pex12 function as protein-ubiquitin ligases in peroxisomal protein import. Mol Cell Biol. 2009;29:5505-16 pubmed publisher
    ..Our results show that Pex2 mediates the Ubc4-dependent polyubiquitination whereas Pex12 facilitates the Pex4-dependent monoubiquitination of Pex5. ..
  4. Lam S, Yoda N, Schekman R. A vesicle carrier that mediates peroxisome protein traffic from the endoplasmic reticulum. Proc Natl Acad Sci U S A. 2010;107:21523-8 pubmed publisher
    ..soluble cytoplasmic transport protein, is required for the traffic of the peroxisomal membrane proteins Pex3p and Pex15p from the endoplasmic reticulum (ER) to the peroxisome...
  5. Weir N, Kamber R, Martenson J, Denic V. The AAA protein Msp1 mediates clearance of excess tail-anchored proteins from the peroxisomal membrane. elife. 2017;6: pubmed publisher
    ..In sum, our work argues that Msp1 selects its substrates on the basis of their solitary membrane existence. ..
  6. Eckert J, Johnsson N. Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome. J Cell Sci. 2003;116:3623-34 pubmed
    ..A yeast strain harboring a deletion of PEX3 enabled us to estimate the influence of the peroxisomal membrane on the formation of a subset of the investigated protein-protein interactions. ..
  7. Platta H, Grunau S, Rosenkranz K, Girzalsky W, Erdmann R. Functional role of the AAA peroxins in dislocation of the cycling PTS1 receptor back to the cytosol. Nat Cell Biol. 2005;7:817-22 pubmed
    ..Here we demonstrate that the AAA peroxins mediate the ATP-dependent dislocation of the peroxisomal targeting signal-1 (PTS1) receptor from the peroxisomal membrane to the cytosol. ..
  8. Rosenkranz K, Birschmann I, Grunau S, Girzalsky W, Kunau W, Erdmann R. Functional association of the AAA complex and the peroxisomal importomer. FEBS J. 2006;273:3804-15 pubmed
    ..form a stable complex in the cytosol, which associates at the peroxisomal membrane with their membrane anchor Pex15p and the peroxisomal importomer...
  9. Oeljeklaus S, Reinartz B, Wolf J, Wiese S, Tonillo J, Podwojski K, et al. Identification of core components and transient interactors of the peroxisomal importomer by dual-track stable isotope labeling with amino acids in cell culture analysis. J Proteome Res. 2012;11:2567-80 pubmed publisher
    ..all known Pex14p interaction partners including the core constituents of the importomer as well as Pex5p, Pex11p, Pex15p, and Dyn2p...

More Information

Publications15

  1. Gardner B, Chowdhury S, Lander G, Martin A. The Pex1/Pex6 complex is a heterohexameric AAA+ motor with alternating and highly coordinated subunits. J Mol Biol. 2015;427:1375-88 pubmed publisher
    ..ATP hydrolysis by Pex1 is highly coordinated with that of Pex6. Furthermore, Pex15, the membrane anchor required for Pex1/Pex6 recruitment to peroxisomes, inhibits the ATP-hydrolysis activity of Pex1/Pex6. ..
  2. Buentzel J, Vilardi F, Lotz Havla A, Gärtner J, Thoms S. Conserved targeting information in mammalian and fungal peroxisomal tail-anchored proteins. Sci Rep. 2015;5:17420 pubmed publisher
    ..These data show that conserved targeting information is employed in yeast and higher eukaryotes during the biogenesis of peroxisomal tail-anchored proteins. ..
  3. Schuldiner M, Metz J, Schmid V, Denic V, Rakwalska M, Schmitt H, et al. The GET complex mediates insertion of tail-anchored proteins into the ER membrane. Cell. 2008;134:634-45 pubmed publisher
    ..Thus, the GET complex represents a critical mechanism for ensuring efficient and accurate targeting of TA proteins. ..
  4. Nuttall J, Motley A, Hettema E. Deficiency of the exportomer components Pex1, Pex6, and Pex15 causes enhanced pexophagy in Saccharomyces cerevisiae. Autophagy. 2014;10:835-45 pubmed publisher
    ..We find Atg36 is modified in pex1? cells even when Atg11 binding is prevented, suggesting Atg36 modification is an early event in the degradation of dysfunctional peroxisomal structures in pex1? cells via pexophagy...
  5. Grimm I, Saffian D, Girzalsky W, Erdmann R. Nucleotide-dependent assembly of the peroxisomal receptor export complex. Sci Rep. 2016;6:19838 pubmed publisher
    ..In this study, we analyzed the recruitment of the AAA-complex to its anchor protein Pex15p at the peroxisomal membrane...
  6. Gardner B, Castanzo D, Chowdhury S, Stjepanovic G, Stefely M, Hurley J, et al. The peroxisomal AAA-ATPase Pex1/Pex6 unfolds substrates by processive threading. Nat Commun. 2018;9:135 pubmed publisher
    ..Our results thus support a role of Pex1/Pex6 in mechanical unfolding of peroxins or their extraction from the peroxisomal membrane during matrix-protein import. ..