PEX14

Summary

Gene Symbol: PEX14
Description: Pex14p
Alias: Pex14p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Brocard C, Lametschwandtner G, Koudelka R, Hartig A. Pex14p is a member of the protein linkage map of Pex5p. EMBO J. 1997;16:5491-500 pubmed
    ..Among the five interaction partners identified, Pex14p was found to be induced under conditions allowing peroxisome proliferation...
  2. Albertini M, Rehling P, Erdmann R, Girzalsky W, Kiel J, Veenhuis M, et al. Pex14p, a peroxisomal membrane protein binding both receptors of the two PTS-dependent import pathways. Cell. 1997;89:83-92 pubmed
    b>Pex14p, an S. cerevisiae peroxin, is attached to the outer face of the peroxisomal membrane and is a component of the protein import machinery. Pex14p interacts with both the PTS1 and PTS2 receptors...
  3. Huhse B, Rehling P, Albertini M, Blank L, Meller K, Kunau W. Pex17p of Saccharomyces cerevisiae is a novel peroxin and component of the peroxisomal protein translocation machinery. J Cell Biol. 1998;140:49-60 pubmed
    ..Two-hybrid studies showed that Pex17p directly binds to Pex14p, the recently proposed point of convergence for the two peroxisomal targeting signal (PTS)-dependent import ..
  4. Bottger G, Barnett P, Klein A, Kragt A, Tabak H, Distel B. Saccharomyces cerevisiae PTS1 receptor Pex5p interacts with the SH3 domain of the peroxisomal membrane protein Pex13p in an unconventional, non-PXXP-related manner. Mol Biol Cell. 2000;11:3963-76 pubmed
    ..membrane protein Pex13p, which contains an Src homology 3 (SH3) domain, and the peripheral membrane protein Pex14p. In the yeast Saccharomyces cerevisiae, both Pex5p and Pex14p are able to bind Pex13p via its SH3 domain...
  5. Agne B, Meindl N, Niederhoff K, Einwächter H, Rehling P, Sickmann A, et al. Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. Mol Cell. 2003;11:635-46 pubmed
    ..have purified and defined two core complexes of the peroxisomal import machinery: the docking complex comprising Pex14p and Pex17p, with the loosely associated Pex13p, and the RING finger complex containing Pex2p, Pex10p, and Pex12p...
  6. de Vries B, Todde V, Stevens P, Salomons F, van der Klei I, Veenhuis M. Pex14p is not required for N-starvation induced microautophagy and in catalytic amounts for macropexophagy in Hansenula polymorpha. Autophagy. 2006;2:183-8 pubmed
    ..biogenesis and selective peroxisome degradation (macropexophagy) converge at the peroxisomal membrane protein Pex14p. Here we show that this protein is not required for peroxisome degradation during nitrogen starvation-induced ..
  7. Platta H, El Magraoui F, Bäumer B, Schlee D, Girzalsky W, Erdmann R. Pex2 and pex12 function as protein-ubiquitin ligases in peroxisomal protein import. Mol Cell Biol. 2009;29:5505-16 pubmed publisher
    ..Our results show that Pex2 mediates the Ubc4-dependent polyubiquitination whereas Pex12 facilitates the Pex4-dependent monoubiquitination of Pex5. ..
  8. Motley A, Galvin P, Ekal L, Nuttall J, Hettema E. Reevaluation of the role of Pex1 and dynamin-related proteins in peroxisome membrane biogenesis. J Cell Biol. 2015;211:1041-56 pubmed publisher
    ..Our data are compatible with the established primary role of the Pex1/Pex6 complex in matrix protein import and show that peroxisomes in Saccharomyces cerevisiae multiply mainly by growth and division. ..
  9. Meinecke M, Cizmowski C, Schliebs W, Krüger V, Beck S, Wagner R, et al. The peroxisomal importomer constitutes a large and highly dynamic pore. Nat Cell Biol. 2010;12:273-7 pubmed publisher
    ..Here, we demonstrate that the membrane-associated import receptor Pex5p together with its docking partner Pex14p forms a gated ion-conducting channel which can be opened to a diameter of about 9 nm by the cytosolic receptor-..

More Information

Publications37

  1. Albertini M, Girzalsky W, Veenhuis M, Kunau W. Pex12p of Saccharomyces cerevisiae is a component of a multi-protein complex essential for peroxisomal matrix protein import. Eur J Cell Biol. 2001;80:257-70 pubmed
    ..receptor Pex5p and the docking proteins for the PTS1 and the PTS2 receptor at the peroxisomal membrane, Pex13p and Pex14p. Furthermore, two-hybrid experiments suggest that the two RING finger domains are sufficient for the Pex10p-Pex12p ..
  2. Montilla Martinez M, Beck S, Klümper J, Meinecke M, Schliebs W, Wagner R, et al. Distinct Pores for Peroxisomal Import of PTS1 and PTS2 Proteins. Cell Rep. 2015;13:2126-34 pubmed publisher
    ..A pore consisting of the PTS1 receptor Pex5 and the docking protein Pex14 imports PTS1 proteins...
  3. Oeljeklaus S, Reinartz B, Wolf J, Wiese S, Tonillo J, Podwojski K, et al. Identification of core components and transient interactors of the peroxisomal importomer by dual-track stable isotope labeling with amino acids in cell culture analysis. J Proteome Res. 2012;11:2567-80 pubmed publisher
    ..A central part of this highly dynamic import machinery is the docking complex consisting of Pex14p, Pex13p, and Pex17p that is linked to the RING finger complex (Pex2p, Pex10p, Pex12p) via Pex8p...
  4. Girzalsky W, Rehling P, Stein K, Kipper J, Blank L, Kunau W, et al. Involvement of Pex13p in Pex14p localization and peroxisomal targeting signal 2-dependent protein import into peroxisomes. J Cell Biol. 1999;144:1151-62 pubmed
    ..b>Pex14p interacts with both the PTS1- and PTS2-receptor and may represent the point of convergence of the PTS1- and PTS2-..
  5. Gardner B, Castanzo D, Chowdhury S, Stjepanovic G, Stefely M, Hurley J, et al. The peroxisomal AAA-ATPase Pex1/Pex6 unfolds substrates by processive threading. Nat Commun. 2018;9:135 pubmed publisher
    ..Our results thus support a role of Pex1/Pex6 in mechanical unfolding of peroxins or their extraction from the peroxisomal membrane during matrix-protein import. ..
  6. Williams C, van den Berg M, Distel B. Saccharomyces cerevisiae Pex14p contains two independent Pex5p binding sites, which are both essential for PTS1 protein import. FEBS Lett. 2005;579:3416-20 pubmed
    b>Pex14p is a peroxisomal membrane-associated protein involved in docking of both Pex5p and Pex7p to the peroxisomal membrane...
  7. Chan A, Schummer A, Fischer S, Schröter T, Cruz Zaragoza L, Bender J, et al. Pex17p-dependent assembly of Pex14p/Dyn2p-subcomplexes of the peroxisomal protein import machinery. Eur J Cell Biol. 2016;95:585-597 pubmed publisher
    ..The docking-complex is composed of Pex13p, Pex14p and in yeast also Pex17p, whose function is still elusive...
  8. Stein K, Schell Steven A, Erdmann R, Rottensteiner H. Interactions of Pex7p and Pex18p/Pex21p with the peroxisomal docking machinery: implications for the first steps in PTS2 protein import. Mol Cell Biol. 2002;22:6056-69 pubmed
    ..Pex7p/cargo complex is transported from the cytosol to the peroxisomal docking complex, consisting of Pex13p and Pex14p. In Saccharomyces cerevisiae, the latter event is thought to require the redundant Pex18p and Pex21p...
  9. Niederhoff K, Meindl Beinker N, Kerssen D, Perband U, Schafer A, Schliebs W, et al. Yeast Pex14p possesses two functionally distinct Pex5p and one Pex7p binding sites. J Biol Chem. 2005;280:35571-8 pubmed
    ..In a first step of a structure-function analysis of Saccharomyces cerevisiae Pex14p, we mapped its binding sites with both receptors...
  10. van der Zand A, Gent J, Braakman I, Tabak H. Biochemically distinct vesicles from the endoplasmic reticulum fuse to form peroxisomes. Cell. 2012;149:397-409 pubmed publisher
    ..Our findings demonstrate a remarkable mechanism to maintain biochemical identity of organelles by transporting crucial components via different routes to their final destination. ..
  11. Effelsberg D, Cruz Zaragoza L, Schliebs W, Erdmann R. Pex9p is a new yeast peroxisomal import receptor for PTS1-containing proteins. J Cell Sci. 2016;129:4057-4066 pubmed
    ..The new peroxin was designated Pex9p, and it interacts with the docking protein Pex14p and a subclass of PTS1-containing peroxisomal matrix enzymes...
  12. Douangamath A, Filipp F, Klein A, Barnett P, Zou P, Voorn Brouwer T, et al. Topography for independent binding of alpha-helical and PPII-helical ligands to a peroxisomal SH3 domain. Mol Cell. 2002;10:1007-17 pubmed
    ..the peroxisomal Pex13p SH3 domain has the unique capacity of binding to two different ligands, Pex5p and Pex14p. We have used this domain as a model to decipher its structurally independent ligand binding sites...
  13. Hensel A, Beck S, El Magraoui F, Platta H, Girzalsky W, Erdmann R. Cysteine-dependent ubiquitination of Pex18p is linked to cargo translocation across the peroxisomal membrane. J Biol Chem. 2011;286:43495-505 pubmed publisher
    ..This finding indicates that Pex18p export is linked to cargo translocation, which supports the idea of an export-driven import of proteins into peroxisomes. ..
  14. Rosenkranz K, Birschmann I, Grunau S, Girzalsky W, Kunau W, Erdmann R. Functional association of the AAA complex and the peroxisomal importomer. FEBS J. 2006;273:3804-15 pubmed
  15. Stelter P, Kunze R, Flemming D, Höpfner D, Diepholz M, Philippsen P, et al. Molecular basis for the functional interaction of dynein light chain with the nuclear-pore complex. Nat Cell Biol. 2007;9:788-96 pubmed
    ..Thus, it is possible that dynein light chain plays a role in organizing natively unfolded Phe-Gly repeats within the NPC scaffold to facilitate nucleocytoplasmic transport. ..
  16. Grunau S, Schliebs W, Linnepe R, Neufeld C, Cizmowski C, Reinartz B, et al. Peroxisomal targeting of PTS2 pre-import complexes in the yeast Saccharomyces cerevisiae. Traffic. 2009;10:451-60 pubmed publisher
    ..membrane-bound subcomplexes suggests that Pex13p provides the initial binding partner at the peroxisomal membrane, whereas Pex14p assembles with Pex18p in high-molecular-weight complexes after or during dissociation of the PTS2 receptor.
  17. Tam Y, Fagarasanu A, Fagarasanu M, Rachubinski R. Pex3p initiates the formation of a preperoxisomal compartment from a subdomain of the endoplasmic reticulum in Saccharomyces cerevisiae. J Biol Chem. 2005;280:34933-9 pubmed
    ..Peroxisome formation from the preperoxisomal compartment depends on the activity of the genes PEX14 and PEX19, which are required for the targeting of peroxisomal matrix and membrane proteins, respectively...
  18. Effelsberg D, Cruz Zaragoza L, Tonillo J, Schliebs W, Erdmann R. Role of Pex21p for Piggyback Import of Gpd1p and Pnc1p into Peroxisomes of Saccharomyces cerevisiae. J Biol Chem. 2015;290:25333-42 pubmed publisher
    ..Moreover, the specific transport of these two enzymes into peroxisomes suggests a novel regulatory role for peroxisomes under various stress conditions. ..
  19. Eckert J, Johnsson N. Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome. J Cell Sci. 2003;116:3623-34 pubmed
    ..A yeast strain harboring a deletion of PEX3 enabled us to estimate the influence of the peroxisomal membrane on the formation of a subset of the investigated protein-protein interactions. ..
  20. Deckers M, Emmrich K, Girzalsky W, Awa W, Kunau W, Erdmann R. Targeting of Pex8p to the peroxisomal importomer. Eur J Cell Biol. 2010;89:924-31 pubmed publisher
  21. Zarrinpar A, Park S, Lim W. Optimization of specificity in a cellular protein interaction network by negative selection. Nature. 2003;426:676-80 pubmed
    ..System-wide negative selection is a subtle but powerful evolutionary mechanism to optimize specificity within an interaction network composed of overlapping recognition elements. ..
  22. Schafer A, Kerssen D, Veenhuis M, Kunau W, Schliebs W. Functional similarity between the peroxisomal PTS2 receptor binding protein Pex18p and the N-terminal half of the PTS1 receptor Pex5p. Mol Cell Biol. 2004;24:8895-906 pubmed
    ..On the basis of these results, we propose that the auxiliary proteins of the PTS2 import pathway fulfill roles similar to those of the N-terminal half of Pex5p in the PTS1 import pathway. ..
  23. Wróblewska J, Cruz Zaragoza L, Yuan W, Schummer A, Chuartzman S, de Boer R, et al. Saccharomyces cerevisiae cells lacking Pex3 contain membrane vesicles that harbor a subset of peroxisomal membrane proteins. Biochim Biophys Acta Mol Cell Res. 2017;1864:1656-1667 pubmed publisher
    ..microscopy and biochemical approaches, we show that a subset of the PMPs, including the receptor docking protein Pex14, localizes to membrane vesicles in S. cerevisiae pex3 cells...
  24. Barnett P, Bottger G, Klein A, Tabak H, Distel B. The peroxisomal membrane protein Pex13p shows a novel mode of SH3 interaction. EMBO J. 2000;19:6382-91 pubmed
    ..On the basis of a structural model of the Pex13p-SH3 domain we show that this interaction probably takes place between the RT- and distal loops. Thus, the Pex13p-SH3-Pex5p interaction establishes a novel mode of SH3 interaction. ..
  25. Schell Steven A, Stein K, Amoros M, Landgraf C, Volkmer Engert R, Rottensteiner H, et al. Identification of a novel, intraperoxisomal pex14-binding site in pex13: association of pex13 with the docking complex is essential for peroxisomal matrix protein import. Mol Cell Biol. 2005;25:3007-18 pubmed
    ..The core protein of this complex, Pex14, is thought to represent the initial docking site for the import receptors Pex5 and Pex7...
  26. Grimm I, Saffian D, Girzalsky W, Erdmann R. Nucleotide-dependent assembly of the peroxisomal receptor export complex. Sci Rep. 2016;6:19838 pubmed publisher
    ..The data indicate that receptor release in peroxisomal protein import is associated with a nucleotide-depending Pex1/6p-cycle of Pex15p-binding and release. ..
  27. Kerssen D, Hambruch E, Klaas W, Platta H, de Kruijff B, Erdmann R, et al. Membrane association of the cycling peroxisome import receptor Pex5p. J Biol Chem. 2006;281:27003-15 pubmed
    ..conserved tryptophan residue within a reverse WXXXF motif abolished two-hybrid binding with the N-terminal half of Pex14p. In combination with an additional mutation introduced into the Pex13p-binding site, we generated a Pex5p mutant ..
  28. Girzalsky W, Hoffmann L, Schemenewitz A, Nolte A, Kunau W, Erdmann R. Pex19p-dependent targeting of Pex17p, a peripheral component of the peroxisomal protein import machinery. J Biol Chem. 2006;281:19417-25 pubmed
    ..We have demonstrated that Pex17p is associated with the peroxisomal Pex13p-Pex14p complex as well as with Pex19p...