PEX12

Summary

Gene Symbol: PEX12
Description: ubiquitin-protein ligase peroxin 12
Alias: PAS11, ubiquitin-protein ligase peroxin 12
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Kerssen D, Hambruch E, Klaas W, Platta H, de Kruijff B, Erdmann R, et al. Membrane association of the cycling peroxisome import receptor Pex5p. J Biol Chem. 2006;281:27003-15 pubmed
    ..Altogether, these data strongly suggest that a translocation-competent state of the PTS1 receptor enters the membrane via protein-lipid interactions before it tightly associates with other peroxins. ..
  2. Platta H, El Magraoui F, Bäumer B, Schlee D, Girzalsky W, Erdmann R. Pex2 and pex12 function as protein-ubiquitin ligases in peroxisomal protein import. Mol Cell Biol. 2009;29:5505-16 pubmed publisher
    ..It is demonstrated that each of the three RING peroxins Pex2, Pex10, and Pex12 exhibits ubiquitin-protein isopeptide ligase activity...
  3. El Magraoui F, Bäumer B, Platta H, Baumann J, Girzalsky W, Erdmann R. The RING-type ubiquitin ligases Pex2p, Pex10p and Pex12p form a heteromeric complex that displays enhanced activity in an ubiquitin conjugating enzyme-selective manner. FEBS J. 2012;279:2060-70 pubmed publisher
    ..The RING domains proved to function as heteromeric pairs that display an Pex10p-dependent enhanced ligase activity in an ubiquitin conjugating enzyme-selective manner. ..
  4. Kiel J, Emmrich K, Meyer H, Kunau W. Ubiquitination of the peroxisomal targeting signal type 1 receptor, Pex5p, suggests the presence of a quality control mechanism during peroxisomal matrix protein import. J Biol Chem. 2005;280:1921-30 pubmed
    ..Our data suggest that during receptor recycling a portion of Pex5p becomes ubiquitinated and degraded by the proteasome. We propose that this process represents a conserved quality control mechanism in peroxisome biogenesis. ..
  5. El Magraoui F, Brinkmeier R, Schrötter A, Girzalsky W, Müller T, Marcus K, et al. Distinct ubiquitination cascades act on the peroxisomal targeting signal type 2 co-receptor Pex18p. Traffic. 2013;14:1290-301 pubmed publisher
    ..Our findings for the PTS2-pathway complement the data on PTS1-receptor ubiquitination and add up to a unified concept of the ubiquitin-based regulation of peroxisomal import. ..
  6. Chang C, Lee W, Moser H, Valle D, Gould S. Isolation of the human PEX12 gene, mutated in group 3 of the peroxisome biogenesis disorders. Nat Genet. 1997;15:385-8 pubmed
    ..Although its sequence similarity to yeast Pex12 proteins was limited, PEX12 shared the same subcellular distribution as yeast Pex12p and localized to the ..
  7. Grimm I, Saffian D, Girzalsky W, Erdmann R. Nucleotide-dependent assembly of the peroxisomal receptor export complex. Sci Rep. 2016;6:19838 pubmed publisher
    ..The data indicate that receptor release in peroxisomal protein import is associated with a nucleotide-depending Pex1/6p-cycle of Pex15p-binding and release. ..
  8. Schafer A, Kerssen D, Veenhuis M, Kunau W, Schliebs W. Functional similarity between the peroxisomal PTS2 receptor binding protein Pex18p and the N-terminal half of the PTS1 receptor Pex5p. Mol Cell Biol. 2004;24:8895-906 pubmed
    ..On the basis of these results, we propose that the auxiliary proteins of the PTS2 import pathway fulfill roles similar to those of the N-terminal half of Pex5p in the PTS1 import pathway. ..
  9. Mano S, Nakamori C, Nito K, Kondo M, Nishimura M. The Arabidopsis pex12 and pex13 mutants are defective in both PTS1- and PTS2-dependent protein transport to peroxisomes. Plant J. 2006;47:604-18 pubmed
    ..APM2 and APM4 were found to encode proteins homologous to the peroxins PEX13 and PEX12, respectively, which are thought to be involved in transporting matrix proteins into peroxisomes in yeasts and ..

More Information

Publications18

  1. Rucktäschel R, Thoms S, Sidorovitch V, Halbach A, Pechlivanis M, Volkmer R, et al. Farnesylation of pex19p is required for its structural integrity and function in peroxisome biogenesis. J Biol Chem. 2009;284:20885-96 pubmed publisher
    ..Thus, isoprenylation of Pex19p contributes to substrate membrane protein recognition for the topogenesis of PMPs, and our results highlight the importance of lipid modifications in protein-protein interactions. ..
  2. Deckers M, Emmrich K, Girzalsky W, Awa W, Kunau W, Erdmann R. Targeting of Pex8p to the peroxisomal importomer. Eur J Cell Biol. 2010;89:924-31 pubmed publisher
  3. van der Zand A, Gent J, Braakman I, Tabak H. Biochemically distinct vesicles from the endoplasmic reticulum fuse to form peroxisomes. Cell. 2012;149:397-409 pubmed publisher
    ..Our findings demonstrate a remarkable mechanism to maintain biochemical identity of organelles by transporting crucial components via different routes to their final destination. ..
  4. Chan A, Schummer A, Fischer S, Schröter T, Cruz Zaragoza L, Bender J, et al. Pex17p-dependent assembly of Pex14p/Dyn2p-subcomplexes of the peroxisomal protein import machinery. Eur J Cell Biol. 2016;95:585-597 pubmed publisher
    ..As dyn2? cells also display reduced peroxisomal function, our data indicate that Dyn2p-dependent formation of the high molecular weight Pex14p-complex is required to maintain peroxisomal function on wild-type level. ..
  5. El Magraoui F, Schrötter A, Brinkmeier R, Kunst L, Mastalski T, Müller T, et al. The cytosolic domain of Pex22p stimulates the Pex4p-dependent ubiquitination of the PTS1-receptor. PLoS ONE. 2014;9:e105894 pubmed publisher
  6. Oeljeklaus S, Reinartz B, Wolf J, Wiese S, Tonillo J, Podwojski K, et al. Identification of core components and transient interactors of the peroxisomal importomer by dual-track stable isotope labeling with amino acids in cell culture analysis. J Proteome Res. 2012;11:2567-80 pubmed publisher
  7. Albertini M, Girzalsky W, Veenhuis M, Kunau W. Pex12p of Saccharomyces cerevisiae is a component of a multi-protein complex essential for peroxisomal matrix protein import. Eur J Cell Biol. 2001;80:257-70 pubmed
    We have isolated the Saccharomyces cerevisiae pex12-1 mutant from a screen to identify mutants defective in peroxisome biogenesis...
  8. Eckert J, Johnsson N. Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome. J Cell Sci. 2003;116:3623-34 pubmed
    ..A yeast strain harboring a deletion of PEX3 enabled us to estimate the influence of the peroxisomal membrane on the formation of a subset of the investigated protein-protein interactions. ..
  9. Agne B, Meindl N, Niederhoff K, Einwächter H, Rehling P, Sickmann A, et al. Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. Mol Cell. 2003;11:635-46 pubmed
    ..We conclude that Pex8p organizes the formation of the larger import complex from the trans side of the peroxisomal membrane and thus might enable functional communication between both sides of the membrane. ..