Gene Symbol: PEP1
Description: type I sorting receptor
Alias: VPS10, VPT1, type I sorting receptor
Species: Saccharomyces cerevisiae S288c
Products:     PEP1

Top Publications

  1. Marcusson E, Horazdovsky B, Cereghino J, Gharakhanian E, Emr S. The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene. Cell. 1994;77:579-86 pubmed
    The S. cerevisiae VPS10 (vacuolar protein sorting) gene encodes a type I transmembrane protein of 1577 amino acids required for the sorting of the soluble vacuolar protein carboxypeptidase Y (CPY)...
  2. Conibear E, Stevens T. Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi. Mol Biol Cell. 2000;11:305-23 pubmed
    ..The Vps52/53/54 complex joins a growing list of distinct multisubunit complexes that regulate membrane-trafficking events. ..
  3. Deloche O, Yeung B, Payne G, Schekman R. Vps10p transport from the trans-Golgi network to the endosome is mediated by clathrin-coated vesicles. Mol Biol Cell. 2001;12:475-85 pubmed
    ..The Vps10p C-terminal domain appears to play a principal role in retrieval of Vps10p from the prevacuolar compartment rather than in sorting from the TGN...
  4. Reddy J, Seaman M. Vps26p, a component of retromer, directs the interactions of Vps35p in endosome-to-Golgi retrieval. Mol Biol Cell. 2001;12:3242-56 pubmed
    ..These domain swap experiments have shown that Vps26p promotes the interactions between the cargo-selective component Vps35p and the structural components Vps5p/Vps17p. ..
  5. Kruse K, Brodsky J, McCracken A. Characterization of an ERAD gene as VPS30/ATG6 reveals two alternative and functionally distinct protein quality control pathways: one for soluble Z variant of human alpha-1 proteinase inhibitor (A1PiZ) and another for aggregates of A1PiZ. Mol Biol Cell. 2006;17:203-12 pubmed
    ..These findings illustrate the complex nature of protein quality control in the secretion pathway and reveal multiple sites that recognize and sort both soluble and aggregated forms of aberrant or misfolded proteins. ..
  6. Li Y, Kane T, Tipper C, Spatrick P, Jenness D. Yeast mutants affecting possible quality control of plasma membrane proteins. Mol Cell Biol. 1999;19:3588-99 pubmed
    ..STP26 mutation also caused missorting of carboxypeptidase Y, and ste2-3 was suppressed by mutations vps1, vps8, vps10, and vps28 but not by mutation vps3...
  7. Whyte J, Munro S. A yeast homolog of the mammalian mannose 6-phosphate receptors contributes to the sorting of vacuolar hydrolases. Curr Biol. 2001;11:1074-8 pubmed
    ..on their N-glycans, and the only sorting receptor so far identified in this organism is the product of the VPS10 gene...
  8. Gokool S, Tattersall D, Reddy J, Seaman M. Identification of a conserved motif required for Vps35p/Vps26p interaction and assembly of the retromer complex. Biochem J. 2007;408:287-95 pubmed
    ..These data implicate Vps26p/VPS26 in regulating Vps35p/VPS35 membrane association and therefore suggest a role for Vps26p/VPS26 in cargo recognition. ..
  9. De M, Abazeed M, Fuller R. Direct binding of the Kex2p cytosolic tail to the VHS domain of yeast Gga2p facilitates TGN to prevacuolar compartment transport and is regulated by phosphorylation. Mol Biol Cell. 2013;24:495-509 pubmed publisher
    ..Thus yeast Gga adaptors facilitate TGN-PVC transport by direct binding of noncanonical phosphoregulated Gga-binding sites in cargo molecules. ..

More Information


  1. Westphal V, Marcusson E, Winther J, Emr S, van den Hazel H. Multiple pathways for vacuolar sorting of yeast proteinase A. J Biol Chem. 1996;271:11865-70 pubmed
    ..This was unexpected, as strains disrupted for VPS10 sort more than 85% of the proteinase A to the vacuole...
  2. Nothwehr S, Ha S, Bruinsma P. Sorting of yeast membrane proteins into an endosome-to-Golgi pathway involves direct interaction of their cytosolic domains with Vps35p. J Cell Biol. 2000;151:297-310 pubmed
    ..These data strongly support a model in which Vps35p acts as a "receptor" protein for recognition of the retrieval signal domains of cargo proteins during their recruitment into retrograde vesicles. ..
  3. Möckli N, Deplazes A, Hassa P, Zhang Z, Peter M, Hottiger M, et al. Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins. Biotechniques. 2007;42:725-30 pubmed
    ..The cytoY2H system extends existing methods for the detection of protein interactions by providing a convenient solution for screening a wide range of transcriptionally active proteins. ..
  4. Deloche O, Schekman R. Vps10p cycles between the TGN and the late endosome via the plasma membrane in clathrin mutants. Mol Biol Cell. 2002;13:4296-307 pubmed
    ..Once relieved of cargo protein, Vps10p would be recycled to the trans-Golgi and then to the cell surface for further rounds of sorting. ..
  5. Gharakhanian E, Chima Okereke O, Olson D, Frost C, Kathleen Takahashi M. env1 Mutant of VPS35 gene exhibits unique protein localization and processing phenotype at Golgi and lysosomal vacuole in Saccharomyces cerevisiae. Mol Cell Biochem. 2011;346:187-95 pubmed publisher