Gene Symbol: PDR3
Description: drug-responsive transcription factor PDR3
Alias: AMY2, TPE2, drug-responsive transcription factor PDR3
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Katzmann D, Hallstrom T, Mahe Y, Moye Rowley W. Multiple Pdr1p/Pdr3p binding sites are essential for normal expression of the ATP binding cassette transporter protein-encoding gene PDR5. J Biol Chem. 1996;271:23049-54 pubmed
    ..A single PDRE placed upstream of a yeast gene lacking its normal upstream activation sequence is sufficient to confer Pdr1p responsiveness to this heterologous promoter...
  2. Zhang X, Kolaczkowska A, Devaux F, Panwar S, Hallstrom T, Jacq C, et al. Transcriptional regulation by Lge1p requires a function independent of its role in histone H2B ubiquitination. J Biol Chem. 2005;280:2759-70 pubmed
    ..PDR5 induction in rho(0) cells requires the presence of the zinc cluster transcription factor Pdr3p. The PDR3 gene is positively autoregulated in rho(0) cells by virtue of the presence of two binding sites for Pdr3p in its ..
  3. Mamnun Y, Pandjaitan R, Mahé Y, Delahodde A, Kuchler K. The yeast zinc finger regulators Pdr1p and Pdr3p control pleiotropic drug resistance (PDR) as homo- and heterodimers in vivo. Mol Microbiol. 2002;46:1429-40 pubmed
  4. Shahi P, Gulshan K, Moye Rowley W. Negative transcriptional regulation of multidrug resistance gene expression by an Hsp70 protein. J Biol Chem. 2007;282:26822-31 pubmed
    ..Finally, rho(0) cells had less Ssa1p bound to Pdr3p than rho(+) cells, consistent with Ssa1p-mediated repression of Pdr3p activity serving as a key regulatory step in control of multidrug resistance in yeast...
  5. Katzmann D, Burnett P, Golin J, Mahe Y, Moye Rowley W. Transcriptional control of the yeast PDR5 gene by the PDR3 gene product. Mol Cell Biol. 1994;14:4653-61 pubmed
    ..drug resistance-encoding PDR mutations have been found that map near the centromere on chromosome II, including PDR3-1 and PDR4-1...
  6. Gulshan K, Schmidt J, Shahi P, Moye Rowley W. Evidence for the bifunctional nature of mitochondrial phosphatidylserine decarboxylase: role in Pdr3-dependent retrograde regulation of PDR5 expression. Mol Cell Biol. 2008;28:5851-64 pubmed publisher
    ..drug resistance genes via increased expression of a zinc cluster-containing transcription factor designated Pdr3. A major Pdr3 target gene is the ATP-binding cassette transporter-encoding gene PDR5...
  7. Delahodde A, Delaveau T, Jacq C. Positive autoregulation of the yeast transcription factor Pdr3p, which is involved in control of drug resistance. Mol Cell Biol. 1995;15:4043-51 pubmed
    ..We provide evidence that the yeast PDR3 gene, which encodes a zinc finger transcription factor implicated in certain drug resistance phenomena, is under ..
  8. Delaveau T, Delahodde A, Carvajal E, Subik J, Jacq C. PDR3, a new yeast regulatory gene, is homologous to PDR1 and controls the multidrug resistance phenomenon. Mol Gen Genet. 1994;244:501-11 pubmed
    The Saccharomyces cerevisiae PDR3 gene, located near the centromere of chromosome II, has been completely sequenced and characterised...
  9. Hallstrom T, Moye Rowley W. Multiple signals from dysfunctional mitochondria activate the pleiotropic drug resistance pathway in Saccharomyces cerevisiae. J Biol Chem. 2000;275:37347-56 pubmed
    ..Both of these mitochondrial defects lead to increased expression of the PDR3 structural gene...

More Information


  1. Sidorova M, Drobna E, Dzugasova V, Hikkel I, Subik J. Loss-of-function pdr3 mutations convert the Pdr3p transcription activator to a protein suppressing multidrug resistance in Saccharomyces cerevisiae. FEMS Yeast Res. 2007;7:254-64 pubmed
    The PDR1 and PDR3 genes encode the main transcription activators involved in the control of multidrug resistance in Saccharomyces cerevisiae...
  2. Anderson J, Ricker N, Sirjusingh C. Antagonism between two mechanisms of antifungal drug resistance. Eukaryot Cell. 2006;5:1243-51 pubmed
    ..generations, populations became fixed for resistance mutations in either of two transcriptional regulators, PDR1 or PDR3. At 400 generations, replicate populations became fixed for another resistance mutation in UNK1, an unmapped gene ..
  3. Delahodde A, Pandjaitan R, Corral Debrinski M, Jacq C. Pse1/Kap121-dependent nuclear localization of the major yeast multidrug resistance (MDR) transcription factor Pdr1. Mol Microbiol. 2001;39:304-12 pubmed
    Pdr1 and Pdr3 are two very similar transcription factors that mainly control membrane biogenesis by adjusting the production of different membrane proteins, such as different ABC or major facilitator superfamily (MFS) transporters...
  4. Borecka Melkusova S, Kozovska Z, Hikkel I, Dzugasova V, Subik J. RPD3 and ROM2 are required for multidrug resistance in Saccharomyces cerevisiae. FEMS Yeast Res. 2008;8:414-24 pubmed publisher
    ..in rom2 Delta mutant cells the drug hypersensitivity and the defect in PDR5 expression were suppressed by PDR1 or PDR3 overexpressed from heterologous promoters and by the hyperactive pdr3-9 mutant allele...
  5. Zhang M, Hanna M, Li J, Butcher S, Dai H, Xiao W. Creation of a hyperpermeable yeast strain to genotoxic agents through combined inactivation of PDR and CWP genes. Toxicol Sci. 2010;113:401-11 pubmed publisher
    ..We argue that this hyperpermeable yeast mutant strain would be suitable for other chemical-based genotoxic assays...
  6. Martens J, Genereaux J, Saleh A, Brandl C. Transcriptional activation by yeast PDR1p is inhibited by its association with NGG1p/ADA3p. J Biol Chem. 1996;271:15884-90 pubmed
    ..Transcriptional activation by a GAL4p DNA binding domain fusion of PDR1p was enhanced in ngg1 and ada2 disruption strains. Similar to its action on GAL4p, the ADA complex acts to inhibit the activation domain of PDR1p. ..
  7. Hahn J, Neef D, Thiele D. A stress regulatory network for co-ordinated activation of proteasome expression mediated by yeast heat shock transcription factor. Mol Microbiol. 2006;60:240-51 pubmed
    ..We show that HSF activates expression of PDR3, encoding a multidrug resistance (MDR) transcription factor that also directly activates RPN4 gene expression...
  8. Miranda M, Masuda C, Ferreira Pereira A, Carvajal E, Ghislain M, Montero Lomeli M. The serine/threonine protein phosphatase Sit4p activates multidrug resistance in Saccharomyces cerevisiae. FEMS Yeast Res. 2010;10:674-86 pubmed publisher
    ..This effect is a consequence of the decreased transcriptional levels of mainly PDR3 and its target genes, PDR5, SNQ2 and YOR1, which encode multidrug efflux pumps...
  9. Schüller C, Mamnun Y, Wolfger H, Rockwell N, Thorner J, Kuchler K. Membrane-active compounds activate the transcription factors Pdr1 and Pdr3 connecting pleiotropic drug resistance and membrane lipid homeostasis in saccharomyces cerevisiae. Mol Biol Cell. 2007;18:4932-44 pubmed
    The Saccharomyces cerevisiae zinc cluster transcription factors Pdr1 and Pdr3 mediate general drug resistance to many cytotoxic substances also known as pleiotropic drug resistance (PDR)...
  10. Kugler K, Jandric Z, Beyer R, Klopf E, Glaser W, Lemmens M, et al. Ribosome quality control is a central protection mechanism for yeast exposed to deoxynivalenol and trichothecin. BMC Genomics. 2016;17:417 pubmed publisher
    ..Our analysis highlights ribosome quality control and a contribution of a histone deacetylase complex as main sources of resistance against DON and TTC. ..
  11. Panwar S, Moye Rowley W. Long chain base tolerance in Saccharomyces cerevisiae is induced by retrograde signals from the mitochondria. J Biol Chem. 2006;281:6376-84 pubmed
    ..Together, these data indicate Pdr3p activation of RSB1 transcription is an important feature of the retrograde response allowing normal detoxification of an endogenous sphingolipid precursor. ..
  12. Galdieri L, Desai P, Vancura A. Facilitated assembly of the preinitiation complex by separated tail and head/middle modules of the mediator. J Mol Biol. 2012;415:464-74 pubmed publisher
    ..The separated tail module thus represents a promiscuous transcriptional factor that binds to many different promoters and is necessary for derepression of FLR1 in sin4? cells. ..
  13. Shahi P, Gulshan K, Naar A, Moye Rowley W. Differential roles of transcriptional mediator subunits in regulation of multidrug resistance gene expression in Saccharomyces cerevisiae. Mol Biol Cell. 2010;21:2469-82 pubmed publisher
    ..Previous work has established that the multidrug resistance transcription factors Pdr1 and Pdr3 interact with the Mediator component Med15/Gal11 to drive normal levels of expression of the ATP-binding cassette ..
  14. Srikanth C, Chakraborti A, Bachhawat A. Acetaminophen toxicity and resistance in the yeast Saccharomyces cerevisiae. Microbiology. 2005;151:99-111 pubmed
    ..In contrast, resistance mediated by Pdr1p/Pdr3p did not require a functional Yap1p, and revealed a distinct hierarchy in the resistance to acetaminophen...
  15. Khakhina S, Johnson S, Manoharlal R, Russo S, Blugeon C, Lemoine S, et al. Control of Plasma Membrane Permeability by ABC Transporters. Eukaryot Cell. 2015;14:442-53 pubmed publisher
  16. Somboon P, Poonsawad A, Wattanachaisaereekul S, Jensen L, Niimi M, Cheevadhanarak S, et al. Fungicide Xylaria sp. BCC 1067 extract induces reactive oxygen species and activates multidrug resistance system in Saccharomyces cerevisiae. Future Microbiol. 2017;12:417-440 pubmed publisher
    ..Antioxidant regulator Yap1, efflux transporter Pdr5 and ascorbate were pivotal to protect S. cerevisiae from extract cytotoxicity. Xylaria sp. BCC 1067 extract is a potentially valuable source of novel antifungals. ..
  17. Nourani A, Wesolowski Louvel M, Delaveau T, Jacq C, Delahodde A. Multiple-drug-resistance phenomenon in the yeast Saccharomyces cerevisiae: involvement of two hexose transporters. Mol Cell Biol. 1997;17:5453-60 pubmed
    ..to these drugs in the wild-type strain, an effect which is more pronounced in a strain having both PDR1 and PDR3 deleted...
  18. Cary G, Yoon S, Torres C, Wang K, Hays M, Ludlow C, et al. Identification and characterization of a drug-sensitive strain enables puromycin-based translational assays in Saccharomyces cerevisiae. Yeast. 2014;31:167-78 pubmed publisher
    ..Puromycin inhibits the growth of yeast cells mutant for erg6?, pdr1? and pdr3? (EPP) on both solid and liquid media...
  19. Alenquer M, Tenreiro S, Sá Correia I. Adaptive response to the antimalarial drug artesunate in yeast involves Pdr1p/Pdr3p-mediated transcriptional activation of the resistance determinants TPO1 and PDR5. FEMS Yeast Res. 2006;6:1130-9 pubmed
    ..Maximum TPO1 mRNA levels were rapidly reduced to basal values following adaptation of the yeast population to artesunate, while high PDR5 levels were maintained during drug-stressed exponential growth...
  20. Koh J, Hajek P, Bedwell D. Overproduction of PDR3 suppresses mitochondrial import defects associated with a TOM70 null mutation by increasing the expression of TOM72 in Saccharomyces cerevisiae. Mol Cell Biol. 2001;21:7576-86 pubmed
    ..We also identified PDR3, a transcriptional regulator of the pleiotropic drug resistance network, as a multicopy suppressor of the ..
  21. Nishida Aoki N, Mori H, Kuroda K, Ueda M. Activation of the mitochondrial signaling pathway in response to organic solvent stress in yeast. Curr Genet. 2015;61:153-64 pubmed publisher
    ..Therefore, the unique function of Pdr3p in organic solvent stress distinguishes this pathway from the multidrug response. ..
  22. Wolfger H, Mahe Y, Parle McDermott A, Delahodde A, Kuchler K. The yeast ATP binding cassette (ABC) protein genes PDR10 and PDR15 are novel targets for the Pdr1 and Pdr3 transcriptional regulators. FEBS Lett. 1997;418:269-74 pubmed
    The yeast transcription factors Pdr1 and Pdr3 control pleiotropic drug resistance (PDR) development, since they regulate expression of ATP-binding cassette (ABC) drug efflux pumps through binding to cis-acting sites known as PDREs (PDR ..
  23. DeRisi J, van den Hazel B, Marc P, Balzi E, Brown P, Jacq C, et al. Genome microarray analysis of transcriptional activation in multidrug resistance yeast mutants. FEBS Lett. 2000;470:156-60 pubmed
    ..Twenty-six overexpressed targets of the PDR1-3 and/or PDR3-7 mutants were identified. Twenty-one are new targets, the majority of which are of unknown function...
  24. Souid A, Gao C, Wang L, Milgrom E, Shen W. ELM1 is required for multidrug resistance in Saccharomyces cerevisiae. Genetics. 2006;173:1919-37 pubmed
    ..Collectively, these data show that ELM1, and genes encoding related serine/threonine protein kinases, are required for regulation of multidrug resistance involving, at least in part, control of PDR5 transcription...
  25. Dzugasova V, Borecka S, Batova M, Pilisiova R, Hervayova N, Subik J. Site-directed mutagenesis of Asp853 in Pdr3p transcriptional activator from Saccharomyces cerevisiae. Yeast. 2010;27:277-84 pubmed publisher
    The PDR3 gene encodes one of the main transcriptional activators involved in the control of multidrug resistance in the yeast Saccharomyces cerevisiae...
  26. Banerjee D, Lelandais G, Shukla S, Mukhopadhyay G, Jacq C, Devaux F, et al. Responses of pathogenic and nonpathogenic yeast species to steroids reveal the functioning and evolution of multidrug resistance transcriptional networks. Eukaryot Cell. 2008;7:68-77 pubmed
    ..In S. cerevisiae, 20 of the Pdr1p/Pdr3p target genes, including PDR3 itself, were rapidly induced by progesterone, which mimics the effects of PDR1 gain-of-function alleles...
  27. Cui Z, Shiraki T, Hirata D, Miyakawa T. Yeast gene YRR1, which is required for resistance to 4-nitroquinoline N-oxide, mediates transcriptional activation of the multidrug resistance transporter gene SNQ2. Mol Microbiol. 1998;29:1307-15 pubmed
    ..The gain-of-function mutation of Yrr1-1p was attributable to the duplication of a 12-amino-acid sequence generated near the carboxy terminus...
  28. Teixeira M, Dias P, Simões T, Sá Correia I. Yeast adaptation to mancozeb involves the up-regulation of FLR1 under the coordinate control of Yap1, Rpn4, Pdr3, and Yrr1. Biochem Biophys Res Commun. 2008;367:249-55 pubmed
  29. Ostapenko D, Burton J, Solomon M. Identification of anaphase promoting complex substrates in S. cerevisiae. PLoS ONE. 2012;7:e45895 pubmed publisher
    ..characterize five potential APC/C substrates identified using these approaches: the transcription factors Tos4 and Pdr3; the mRNA processing factor Fir1; the spindle checkpoint protein kinase Mps1; and a protein of unknown function, ..
  30. Legras J, Erny C, Le Jeune C, Lollier M, Adolphe Y, Demuyter C, et al. Activation of two different resistance mechanisms in Saccharomyces cerevisiae upon exposure to octanoic and decanoic acids. Appl Environ Microbiol. 2010;76:7526-35 pubmed publisher
    ..These results are confirmed by the sensitivity of strains bearing deletions for the transcription factors encoded by PDR1, STB5, OAF1, and PIP2 genes. ..
  31. Mutlu N, Garipler G, Akdoğan E, Dunn C. Activation of the pleiotropic drug resistance pathway can promote mitochondrial DNA retention by fusion-defective mitochondria in Saccharomyces cerevisiae. G3 (Bethesda). 2014;4:1247-58 pubmed publisher
    ..suppressor mutations not associated with known mitochondrial division components were localized to PDR1 or PDR3, which encode transcription factors promoting drug resistance...