Gene Symbol: PCK1
Description: phosphoenolpyruvate carboxykinase PCK1
Alias: JPM2, PPC1, phosphoenolpyruvate carboxykinase PCK1
Species: Saccharomyces cerevisiae S288c
Nickas M, Yaffe M. BRO1, a novel gene that interacts with components of the Pkc1p-mitogen-activated protein kinase pathway in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:2585-93 pubmed
..Analysis of the isolated BRO1 gene revealed that it encodes a novel, 97-kDa polypeptide which contains a putative SH3 domain-binding motif and is homologous to a protein of unknown function in Caenorhabditis elegans. ..
Cristina Ravanal M, Flores M, Pérez E, Aroca F, Cardemil E. Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase: relevance of arginine 70 for catalysis. Biochimie. 2004;86:357-62 pubmed
..It is proposed that the electrostatic interaction between the guanidinium group of Arg70 and the carboxyl group of PEP is important for PEP binding and for further steps in catalysis. ..
Villarreal J, Bueno C, Arenas F, Jabalquinto A, Gonzalez Nilo F, Encinas M, et al
. Nucleotide specificity of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase Kinetics, fluorescence spectroscopy, and molecular simulation studies. Int J Biochem Cell Biol. 2006;38:576-88 pubmed
..Experimental data obtained with a Thr463Ala mutant enzyme agree with molecular simulation predictions. The results here presented are discussed in terms of the proposed interactions of the nucleotides with the protein. ..
Yevenes A, Espinoza R, Rivas Pardo J, Villarreal J, Gonzalez Nilo F, Cardemil E. Site-directed mutagenesis study of the microenvironment characteristics of Lys213 of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase. Biochimie. 2006;88:663-72 pubmed
..3 kcal mol(-1) in the affinity of the enzyme for PEP, an effect perhaps related to the close proximity of Phe(416) to Arg(70), a residue previously shown to be important for PEP binding. ..
Yevenes A, Gonzalez Nilo F, Cardemil E. Relevance of phenylalanine 216 in the affinity of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase for Mn(II). Protein J. 2007;26:135-41 pubmed
..In agreement with this interpretation, theoretical calculations indicate an alkaline shift of 2.8 pH units in the pK (a) of the epsilon-amino group of Lys(213) upon Phe216Tyr mutation. ..
Tobar I, Gonzalez Nilo F, Jabalquinto A, Cardemil E. Relevance of Arg457 for the nucleotide affinity of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase. Int J Biochem Cell Biol. 2008;40:1883-9 pubmed publisher
..These results show that Arg457 is an important factor for nucleotide binding by S. cerevisiae PEP carboxykinase. ..
Fan X, Martin Brown S, Florens L, Li R. Intrinsic capability of budding yeast cofilin to promote turnover of tropomyosin-bound actin filaments. PLoS ONE. 2008;3:e3641 pubmed publisher
..Our results suggest that yeast cofilin has the intrinsic ability to promote actin cable turnover, and that the severing activity may rely on its ability to bind Tpm1. ..
Haarasilta S, Taskinen L. Location of three key enzymes of gluconeogenesis in baker's yeast. Arch Microbiol. 1977;113:159-61 pubmed
..On the evidence obtained from zonal separations these three enzymes of gluconeogenesis are most probably located in the soluble cytosol...
Valdés Hevia M, de la Guerra R, Gancedo C. Isolation and characterization of the gene encoding phosphoenolpyruvate carboxykinase from Saccharomyces cerevisiae. FEBS Lett. 1989;258:313-6 pubmed
The yeast PCK1 gene coding for phosphoenolpyruvate carboxykinase (PEPCK) was isolated by functional complementation of pck1 strains from S. cerevisiae. Only one copy of the gene was found per haploid yeast genome...