PCH2

Summary

Gene Symbol: PCH2
Description: Pch2p
Alias: Pch2p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. San Segundo P, Roeder G. Pch2 links chromatin silencing to meiotic checkpoint control. Cell. 1999;97:313-24 pubmed
    The PCH2 gene of Saccharomyces cerevisiae is required for the meiotic checkpoint that prevents chromosome segregation when recombination and chromosome synapsis are defective...
  2. Ontoso D, Acosta I, van Leeuwen F, Freire R, San Segundo P. Dot1-dependent histone H3K79 methylation promotes activation of the Mek1 meiotic checkpoint effector kinase by regulating the Hop1 adaptor. PLoS Genet. 2013;9:e1003262 pubmed publisher
    ..Hop1 activation and its proper distribution along zip1 meiotic chromosomes, at least in part, by regulating Pch2 localization. Furthermore, HOP1 overexpression bypasses the Dot1 requirement for checkpoint activation...
  3. Farmer S, Hong E, Leung W, Argunhan B, Terentyev Y, Humphryes N, et al. Budding yeast Pch2, a widely conserved meiotic protein, is involved in the initiation of meiotic recombination. PLoS ONE. 2012;7:e39724 pubmed publisher
    Budding yeast Pch2 protein is a widely conserved meiosis-specific protein whose role is implicated in the control of formation and displacement of meiotic crossover events...
  4. Wu H, Burgess S. Two distinct surveillance mechanisms monitor meiotic chromosome metabolism in budding yeast. Curr Biol. 2006;16:2473-9 pubmed
    ..checkpoint", which in budding yeast requires Rad17, a component of a PCNA clamp-like complex, and Pch2, a putative AAA-ATPase ...
  5. Börner G, Barot A, Kleckner N. Yeast Pch2 promotes domainal axis organization, timely recombination progression, and arrest of defective recombinosomes during meiosis. Proc Natl Acad Sci U S A. 2008;105:3327-32 pubmed publisher
    ..The widely conserved AAA+-ATPase Pch2 mediates this pattern, likely by means of direct intervention along axes...
  6. Zanders S, Sonntag Brown M, Chen C, Alani E. Pch2 modulates chromatid partner choice during meiotic double-strand break repair in Saccharomyces cerevisiae. Genetics. 2011;188:511-21 pubmed publisher
    ..The PCH2 gene of budding yeast is required to establish proper meiotic chromosome axis structure and to regulate meiotic ..
  7. Ho H, Burgess S. Pch2 acts through Xrs2 and Tel1/ATM to modulate interhomolog bias and checkpoint function during meiosis. PLoS Genet. 2011;7:e1002351 pubmed publisher
    ..Repair is biased toward using the homolog as a substrate rather than the sister chromatid. Pch2 is a conserved member of the AAA(+)-ATPase family of proteins and is implicated in a wide range of meiosis-specific ..
  8. Wu H, Ho H, Burgess S. Mek1 kinase governs outcomes of meiotic recombination and the checkpoint response. Curr Biol. 2010;20:1707-16 pubmed publisher
    ..We discuss how regulation of pachytene exit by Mek1 or similar kinases could influence checkpoint stringency, which may differ among species and between sexes. ..
  9. Carballo J, Panizza S, Serrentino M, Johnson A, Geymonat M, Borde V, et al. Budding yeast ATM/ATR control meiotic double-strand break (DSB) levels by down-regulating Rec114, an essential component of the DSB-machinery. PLoS Genet. 2013;9:e1003545 pubmed publisher
    ..Taken together, we present evidence for three independent ways of regulating Rec114 activity, which likely contribute to meiotic DSBs-homeostasis in maintaining genetically determined levels of breaks. ..

More Information

Publications30

  1. Vader G, Blitzblau H, Tame M, Falk J, Curtin L, Hochwagen A. Protection of repetitive DNA borders from self-induced meiotic instability. Nature. 2011;477:115-9 pubmed publisher
    ..this localized DSB susceptibility necessitates a border-specific protection system consisting of the meiotic ATPase Pch2 and the origin recognition complex subunit Orc1...
  2. Zanders S, Alani E. The pch2Delta mutation in baker's yeast alters meiotic crossover levels and confers a defect in crossover interference. PLoS Genet. 2009;5:e1000571 pubmed publisher
    b>Pch2 is a widely conserved protein that is required in baker's yeast for the organization of meiotic chromosome axes into specific domains...
  3. Joshi N, Barot A, Jamison C, Börner G. Pch2 links chromosome axis remodeling at future crossover sites and crossover distribution during yeast meiosis. PLoS Genet. 2009;5:e1000557 pubmed publisher
    ..zygotene stage, persists until double Holliday junction resolution, and is controlled by the conserved AAA+ ATPase Pch2. Pch2 further mediates crossover interference, although it is dispensable for crossover formation at normal levels...
  4. Zierhut C, Berlinger M, Rupp C, Shinohara A, Klein F. Mnd1 is required for meiotic interhomolog repair. Curr Biol. 2004;14:752-62 pubmed
    ..We propose a model in which Mnd1 facilitates chromatin accessibility, which is required to allow strand invasion in meiotic chromatin. ..
  5. San Segundo P, Roeder G. Role for the silencing protein Dot1 in meiotic checkpoint control. Mol Biol Cell. 2000;11:3601-15 pubmed
    ..Other components of the pachytene checkpoint include the nucleolar protein Pch2 and the heterochromatin component Sir2...
  6. Chen C, Jomaa A, Ortega J, ALANI E. Pch2 is a hexameric ring ATPase that remodels the chromosome axis protein Hop1. Proc Natl Acad Sci U S A. 2014;111:E44-53 pubmed publisher
    In budding yeast the pachytene checkpoint 2 (Pch2) protein regulates meiotic chromosome axis structure by maintaining the domain-like organization of the synaptonemal complex proteins homolog pairing 1 (Hop1) and molecular zipper 1 (Zip1)...
  7. Argunhan B, Farmer S, Leung W, Terentyev Y, Humphryes N, Tsubouchi T, et al. Direct and indirect control of the initiation of meiotic recombination by DNA damage checkpoint mechanisms in budding yeast. PLoS ONE. 2013;8:e65875 pubmed publisher
    ..We also identified prophase I exit, which is under the control of the DNA damage checkpoint machinery, to be a critical event associated with down-regulating meiotic DSB formation. ..
  8. Chakraborty P, Pankajam A, Lin G, Dutta A, Krishnaprasad N, Tekkedil M, et al. Modulating Crossover Frequency and Interference for Obligate Crossovers in Saccharomyces cerevisiae Meiosis. G3 (Bethesda). 2017;7:1511-1524 pubmed publisher
    ..are reduced in the mismatch repair related mutant mlh3? and enhanced in a meiotic checkpoint mutant pch2? by up to twofold at specific chromosomal loci, but both mutants maintain high spore viability...
  9. Herruzo E, Ontoso D, González Arranz S, Cavero S, Lechuga A, San Segundo P. The Pch2 AAA+ ATPase promotes phosphorylation of the Hop1 meiotic checkpoint adaptor in response to synaptonemal complex defects. Nucleic Acids Res. 2016;44:7722-41 pubmed publisher
    ..b>Pch2 is an evolutionarily conserved AAA+ ATPase required for the checkpoint-induced meiotic block in the zip1 mutant, ..
  10. Vader G. Pch2(TRIP13): controlling cell division through regulation of HORMA domains. Chromosoma. 2015;124:333-9 pubmed publisher
    ..b>Pch2(TRIP13), a generally conserved member of the AAA(+) ATPase (AAA(+)--ATPases associated with diverse cellular ..
  11. Joshi N, Brown M, Bishop D, Börner G. Gradual implementation of the meiotic recombination program via checkpoint pathways controlled by global DSB levels. Mol Cell. 2015;57:797-811 pubmed publisher
    ..resection and interhomolog processing are controlled by the conserved checkpoint proteins Tel1(ATM) kinase and Pch2(TRIP13) ATPase...
  12. Lo Y, Chuang C, Wang T. Pch2 prevents Mec1/Tel1-mediated Hop1 phosphorylation occurring independently of Red1 in budding yeast meiosis. PLoS ONE. 2014;9:e85687 pubmed publisher
    ..Here, we report that the pachytene checkpoint protein 2 (Pch2) specifically prevents Red1-independent Hop1 phosphorylation...
  13. Qiu Z, Shuman S, Schwer B. An essential role for trimethylguanosine RNA caps in Saccharomyces cerevisiae meiosis and their requirement for splicing of SAE3 and PCH2 meiotic pre-mRNAs. Nucleic Acids Res. 2011;39:5633-46 pubmed publisher
    ..tgs1? cells are specifically defective in splicing PCH2 and SAE3 meiotic pre-mRNAs...
  14. Roig I, Dowdle J, Toth A, de Rooij D, Jasin M, Keeney S. Mouse TRIP13/PCH2 is required for recombination and normal higher-order chromosome structure during meiosis. PLoS Genet. 2010;6: pubmed publisher
    ..The yeast Pch2 protein has emerged as an important factor with roles in both recombination and chromosome structure formation, but ..
  15. Beyer A. Sequence analysis of the AAA protein family. Protein Sci. 1997;6:2043-58 pubmed
    ..The agreement was unexpectedly high and strongly supports the conclusion that the AAA family belongs to the Walker superfamily of A/GTPases. ..
  16. Nishant K, Chen C, Shinohara M, Shinohara A, Alani E. Genetic analysis of baker's yeast Msh4-Msh5 reveals a threshold crossover level for meiotic viability. PLoS Genet. 2010;6: pubmed publisher
    ..allele and mutations that decreased meiotic double-strand break levels (spo11-HA) and crossover interference (pch2?) showed synergistic defects in spore viability...
  17. Cooper K, Mallory M, Guacci V, Lowe K, Strich R. Pds1p is required for meiotic recombination and prophase I progression in Saccharomyces cerevisiae. Genetics. 2009;181:65-79 pubmed publisher
    ..This role is meiosis specific as Mcd1p destruction is not altered in vegetative pds1Delta cultures. These results define a previously undescribed role for Pds1p in cohesin maintenance, recombination, and meiotic progression. ..
  18. Wang Y, Chang C, Wu J, Tung K. Nuclear localization of the meiosis-specific transcription factor Ndt80 is regulated by the pachytene checkpoint. Mol Biol Cell. 2011;22:1878-86 pubmed publisher
    ..We propose that the nuclear localization of Ndt80 is regulated by the pachytene checkpoint through a cytoplasmic anchor mechanism. ..
  19. Qiu Z, Schwer B, Shuman S. Determinants of Nam8-dependent splicing of meiotic pre-mRNAs. Nucleic Acids Res. 2011;39:3427-45 pubmed publisher
    ..Here, we identify SPO22 and PCH2 as novel targets of Nam8-dependent meiotic splicing. Whereas SPO22 splicing is co-dependent on Mer1, PCH2 is not...
  20. Najor N, Weatherford L, Brush G. Prevention of DNA Rereplication Through a Meiotic Recombination Checkpoint Response. G3 (Bethesda). 2016;6:3869-3881 pubmed publisher
    ..While this response is similar to a checkpoint mechanism that inhibits initiation of DNA replication in the mitotic cell cycle, the evidence points to a new variation on DNA replication control. ..
  21. Miao C, Tang D, Zhang H, Wang M, Li Y, Tang S, et al. Central region component1, a novel synaptonemal complex component, is essential for meiotic recombination initiation in rice. Plant Cell. 2013;25:2998-3009 pubmed publisher
    ..CRC1 is orthologous to Saccharomyces cerevisiae pachytene checkpoint2 (Pch2) and Mus musculus THYROID receptor-interacting protein13 (TRIP13) and may be a conserved SC component...