PAT1

Summary

Gene Symbol: PAT1
Description: Pat1p
Alias: MRT1, Pat1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Wyers F, Minet M, Dufour M, Vo L, Lacroute F. Deletion of the PAT1 gene affects translation initiation and suppresses a PAB1 gene deletion in yeast. Mol Cell Biol. 2000;20:3538-49 pubmed
    ..We found that deletion of the PAT1 (YCR077c) gene suppresses a PAB1 gene deletion and that Pat1p is required for the normal initiation of translation...
  2. Lavut A, Raveh D. Sequestration of highly expressed mRNAs in cytoplasmic granules, P-bodies, and stress granules enhances cell viability. PLoS Genet. 2012;8:e1002527 pubmed publisher
    ..ecd3, pat1 double mutants that are defective in P-body formation were sensitive to mRNAs expressed ectopically from strong ..
  3. Lotan R, Bar On V, Harel Sharvit L, Duek L, Melamed D, Choder M. The RNA polymerase II subunit Rpb4p mediates decay of a specific class of mRNAs. Genes Dev. 2005;19:3004-16 pubmed
    ..Moreover, Rpb4p interacts with both the mRNP and with subunits of the mRNA decay complex Pat1/Lsm1-7 that enhances decapping...
  4. Sharif H, Conti E. Architecture of the Lsm1-7-Pat1 complex: a conserved assembly in eukaryotic mRNA turnover. Cell Rep. 2013;5:283-91 pubmed publisher
    The decay of mRNAs is a key step in eukaryotic gene expression. The cytoplasmic Lsm1-7-Pat1 complex is a conserved component of the 5'-to-3' mRNA decay pathway, linking deadenylation to decapping...
  5. Buchan J, Muhlrad D, Parker R. P bodies promote stress granule assembly in Saccharomyces cerevisiae. J Cell Biol. 2008;183:441-55 pubmed publisher
    ..These observations argue that P bodies are important sites for decisions of mRNA fate and that stress granules, at least in yeast, primarily represent pools of mRNAs stalled in the process of reentry into translation from P bodies. ..
  6. Coller J, Parker R. General translational repression by activators of mRNA decapping. Cell. 2005;122:875-86 pubmed
    ..We identify the decapping activators Dhh1p and Pat1p as functioning as translational repressors and facilitators of P body formation...
  7. Tharun S, He W, Mayes A, Lennertz P, Beggs J, Parker R. Yeast Sm-like proteins function in mRNA decapping and decay. Nature. 2000;404:515-8 pubmed
    ..addition, the Lsm1-Lsm7 proteins co-immunoprecipitate with the mRNA decapping enzyme (Dcp1), a decapping activator (Pat1/Mrt1) and with mRNA...
  8. Tharun S, Parker R. Targeting an mRNA for decapping: displacement of translation factors and association of the Lsm1p-7p complex on deadenylated yeast mRNAs. Mol Cell. 2001;8:1075-83 pubmed
    ..These results define an important rearrangement in mRNP organization and suggest that deadenylation promotes mRNA decapping by both the loss of Pab1p and the recruitment of the Lsm1p-7p complex. ..
  9. Balagopal V, Parker R. Stm1 modulates mRNA decay and Dhh1 function in Saccharomyces cerevisiae. Genetics. 2009;181:93-103 pubmed publisher
    ..In the budding yeast Saccharomyces cerevisae, decapping is promoted by the Dhh1 and Pat1 proteins, which appear to both inhibit translation initiation and promote decapping...

More Information

Publications54

  1. Lotan R, Goler Baron V, Duek L, Haimovich G, Choder M. The Rpb7p subunit of yeast RNA polymerase II plays roles in the two major cytoplasmic mRNA decay mechanisms. J Cell Biol. 2007;178:1133-43 pubmed
    ..Moreover, Rpb7p interacts with the decapping regulator Pat1p in a manner important for the mRNA decay machinery...
  2. Fischer N, Weis K. The DEAD box protein Dhh1 stimulates the decapping enzyme Dcp1. EMBO J. 2002;21:2788-97 pubmed
    ..Dhh1 is a cytoplasmic protein and is shown to be in a complex with the mRNA degradation factor Pat1/Mtr1 and with the 5'-3' exoribonuclease Xrn1...
  3. Sheth U, Parker R. Decapping and decay of messenger RNA occur in cytoplasmic processing bodies. Science. 2003;300:805-8 pubmed
    ..These results define the flux of mRNAs between polysomes and P bodies as a critical aspect of cytoplasmic mRNA metabolism and a possible site for regulation of mRNA degradation...
  4. Bonnerot C, Boeck R, Lapeyre B. The two proteins Pat1p (Mrt1p) and Spb8p interact in vivo, are required for mRNA decay, and are functionally linked to Pab1p. Mol Cell Biol. 2000;20:5939-46 pubmed
    ..Coimmunoprecipitation experiments show that Pat1p is associated with Spb8p...
  5. Chowdhury A, Mukhopadhyay J, Tharun S. The decapping activator Lsm1p-7p-Pat1p complex has the intrinsic ability to distinguish between oligoadenylated and polyadenylated RNAs. RNA. 2007;13:998-1016 pubmed
    ..heptameric Lsm1p-7p complex (made up of the seven Sm-like proteins, Lsm1p-Lsm7p) and its interacting partner Pat1p activate decapping by an unknown mechanism and localize with other decapping factors to the P-bodies in the ..
  6. He W, Parker R. The yeast cytoplasmic LsmI/Pat1p complex protects mRNA 3' termini from partial degradation. Genetics. 2001;158:1445-55 pubmed
    ..Recently, a complex of seven Lsm proteins and the Pat1p have been shown to interact with yeast mRNAs and promote mRNA decapping...
  7. Chowdhury A, Tharun S. Activation of decapping involves binding of the mRNA and facilitation of the post-binding steps by the Lsm1-7-Pat1 complex. RNA. 2009;15:1837-48 pubmed publisher
    Decapping is a critical step in the conserved 5'-to-3' mRNA decay pathway of eukaryotes. The hetero-octameric Lsm1-7-Pat1 complex is required for normal rates of decapping in this pathway...
  8. Sharif H, Ozgur S, Sharma K, Basquin C, Urlaub H, Conti E. Structural analysis of the yeast Dhh1-Pat1 complex reveals how Dhh1 engages Pat1, Edc3 and RNA in mutually exclusive interactions. Nucleic Acids Res. 2013;41:8377-90 pubmed publisher
    ..Removal of the 5' cap structure is a crucial step that commits deadenylated mRNAs to 5'-to-3' degradation. Pat1, Edc3 and the DEAD-box protein Dhh1 are evolutionary conserved factors known to participate in both translational ..
  9. Coller J, Tucker M, Sheth U, Valencia Sanchez M, Parker R. The DEAD box helicase, Dhh1p, functions in mRNA decapping and interacts with both the decapping and deadenylase complexes. RNA. 2001;7:1717-27 pubmed
    ..with several proteins involved in mRNA decapping including the decapping enzyme Dcp1p, as well as Lsm1p and Pat1p/Mrt1p, which function to enhance the decapping rate...
  10. Bouveret E, Rigaut G, Shevchenko A, Wilm M, Seraphin B. A Sm-like protein complex that participates in mRNA degradation. EMBO J. 2000;19:1661-71 pubmed
    ..In contrast, the Lsm1p-Lsm7p complex is associated with Pat1p and Xrn1p exoribonuclease, suggesting a role in mRNA degradation...
  11. Nissan T, Rajyaguru P, She M, Song H, Parker R. Decapping activators in Saccharomyces cerevisiae act by multiple mechanisms. Mol Cell. 2010;39:773-83 pubmed publisher
    ..In yeast cells, Pat1, Scd6, Edc3, and Dhh1 all function to promote decapping by an unknown mechanism(s)...
  12. Pilkington G, Parker R. Pat1 contains distinct functional domains that promote P-body assembly and activation of decapping. Mol Cell Biol. 2008;28:1298-312 pubmed
    ..The Pat1 protein is a key component of this complex and an important activator of decapping, yet little is known about its ..
  13. Ramachandran V, Shah K, Herman P. The cAMP-dependent protein kinase signaling pathway is a key regulator of P body foci formation. Mol Cell. 2011;43:973-81 pubmed publisher
    ..suggest that PKA specifically inhibits the formation of the larger P body aggregates by directly phosphorylating Pat1, a conserved constituent of these foci that functions as a scaffold during the assembly process...
  14. Duan R, Rhie B, Ryu H, Ahn S. The RNA polymerase II Rpb4/7 subcomplex regulates cellular lifespan through an mRNA decay process. Biochem Biophys Res Commun. 2013;441:266-70 pubmed
    ..Tandem affinity purification experiments demonstrated that Rpb7 physically associates with Tpk2 and Pat1, which are both implicated in mRNA degradation...
  15. Chowdhury A, Kalurupalle S, Tharun S. Pat1 contributes to the RNA binding activity of the Lsm1-7-Pat1 complex. RNA. 2014;20:1465-75 pubmed publisher
    ..In this pathway, decapping is a rate-limiting step that requires the hetero-octameric Lsm1-7-Pat1 complex to occur at normal rates in vivo...
  16. Wang X, Watt P, Louis E, Borts R, Hickson I. Pat1: a topoisomerase II-associated protein required for faithful chromosome transmission in Saccharomyces cerevisiae. Nucleic Acids Res. 1996;24:4791-7 pubmed
    ..Strains lacking Pat1p exhibit a slow growth rate and a phenotype reminiscent of conditional top2 mutants grown at the semi-permissive ..
  17. Balagopal V, Parker R. Stm1 modulates translation after 80S formation in Saccharomyces cerevisiae. RNA. 2011;17:835-42 pubmed publisher
    ..We demonstrate that in vitro Stm1 inhibits translation after formation of an 80S complex. This suggests that Stm1 modulates translation and mRNA decapping by controlling translation elongation. ..
  18. Cuenca Bono B, García Molinero V, Pascual García P, García Oliver E, Llopis A, Rodriguez Navarro S. A novel link between Sus1 and the cytoplasmic mRNA decay machinery suggests a broad role in mRNA metabolism. BMC Cell Biol. 2010;11:19 pubmed publisher
    ..We provide evidence for genetic interactions between SUS1 and genes coding for components of P-bodies such as PAT1, LSM1, LSM6 and DHH1...
  19. Chowdhury A, Tharun S. lsm1 mutations impairing the ability of the Lsm1p-7p-Pat1p complex to preferentially bind to oligoadenylated RNA affect mRNA decay in vivo. RNA. 2008;14:2149-58 pubmed publisher
    ..The conserved Lsm1p-7p-Pat1p complex is required for normal rates of decapping in vivo, and the purified complex exhibits strong binding ..
  20. Haimovich G, Medina D, Causse S, Garber M, Millán Zambrano G, Barkai O, et al. Gene expression is circular: factors for mRNA degradation also foster mRNA synthesis. Cell. 2013;153:1000-11 pubmed publisher
    ..The gene expression process is therefore circular, whereby the hitherto first and last stages are interconnected. ..
  21. Kurischko C, Kim H, Kuravi V, Pratzka J, Luca F. The yeast Cbk1 kinase regulates mRNA localization via the mRNA-binding protein Ssd1. J Cell Biol. 2011;192:583-98 pubmed publisher
  22. Beckham C, Hilliker A, Cziko A, Noueiry A, Ramaswami M, Parker R. The DEAD-box RNA helicase Ded1p affects and accumulates in Saccharomyces cerevisiae P-bodies. Mol Biol Cell. 2008;19:984-93 pubmed
    ..in increased size and number of P-bodies and inhibition of growth in a manner partially suppressed by loss of Pat1p, Dhh1p, or Lsm1p...
  23. Shively C, Kweon H, Norman K, Mellacheruvu D, Xu T, Sheidy D, et al. Large-Scale Analysis of Kinase Signaling in Yeast Pseudohyphal Development Identifies Regulation of Ribonucleoprotein Granules. PLoS Genet. 2015;11:e1005564 pubmed publisher
    ..Kss1p pathway activity is reduced in lsm1Δ/Δ and pat1Δ/Δ strains, and these genes encoding P-body proteins are epistatic to STE7...
  24. Fourati Z, Kolesnikova O, Back R, Keller J, Charenton C, Taverniti V, et al. The C-terminal domain from S. cerevisiae Pat1 displays two conserved regions involved in decapping factor recruitment. PLoS ONE. 2014;9:e96828 pubmed publisher
    ..Among these factors, Pat1 is considered to be a central scaffolding protein involved in Dcp2 activation but also in inhibition of translation ..
  25. Libuda D, Winston F. Alterations in DNA replication and histone levels promote histone gene amplification in Saccharomyces cerevisiae. Genetics. 2010;184:985-97 pubmed publisher
    ..Taken together, our results suggest that either reduced histone levels or slowed replication forks stimulate the HTA2-HTB2 amplification event, contributing to the restoration of normal chromatin structure. ..
  26. Tharun S. Purification and analysis of the decapping activator Lsm1p-7p-Pat1p complex from yeast. Methods Enzymol. 2008;448:41-55 pubmed publisher
    ..The Lsm1p-7p-Pat1p complex is a key activator of decapping in the 5' to 3'-mRNA decay pathway that is highly conserved in all ..
  27. Duan R, Rhie B, Ryu H, Ahn S. The RNA polymerase II Rpb4/7 subcomplex regulates cellular lifespan through an mRNA decay process. Biochem Biophys Res Commun. 2013;: pubmed publisher
    ..Tandem affinity purification experiments demonstrated that Rpb7 physically associates with Tpk2 and Pat1, which are both implicated in mRNA degradation...
  28. Alhusaini N, Coller J. The deadenylase components Not2p, Not3p, and Not5p promote mRNA decapping. RNA. 2016;22:709-21 pubmed publisher
    ..Furthermore, we find that Not3p and Not5p bind to the decapping activator protein Pat1p. Together, these data implicate the deadenylase complex in coordinating the downstream decapping reaction via ..
  29. Hurto R, Hopper A. P-body components, Dhh1 and Pat1, are involved in tRNA nuclear-cytoplasmic dynamics. RNA. 2011;17:912-24 pubmed publisher
    ..Dhh1 and Pat1 function in parallel to promote translation repression and P-body formation in response to starvation...
  30. Mishra P, Ottmann A, Basrai M. Structural integrity of centromeric chromatin and faithful chromosome segregation requires Pat1. Genetics. 2013;195:369-79 pubmed publisher
    ..Here we report that an evolutionarily conserved protein Pat1 is a structural component of Saccharomyces cerevisiae kinetochore and associates with centromeres in a NDC10-..
  31. Chowdhury A, Raju K, Kalurupalle S, Tharun S. Both Sm-domain and C-terminal extension of Lsm1 are important for the RNA-binding activity of the Lsm1-7-Pat1 complex. RNA. 2012;18:936-44 pubmed publisher
    ..The highly conserved eukaryotic Lsm1 through Lsm7 proteins are part of the cytoplasmic Lsm1-7-Pat1 complex, which is an activator of decapping in the conserved 5'-3' mRNA decay pathway...
  32. Mishra P, Guo J, Dittman L, Haase J, Yeh E, Bloom K, et al. Pat1 protects centromere-specific histone H3 variant Cse4 from Psh1-mediated ubiquitination. Mol Biol Cell. 2015;26:2067-79 pubmed publisher
    ..The kinetochore protein Pat1 regulates the levels and spatial distribution of Cse4 at centromeres...
  33. Kilchert C, Weidner J, Prescianotto Baschong C, Spang A. Defects in the secretory pathway and high Ca2+ induce multiple P-bodies. Mol Biol Cell. 2010;21:2624-38 pubmed publisher
    ..through starvation or osmotic stress, PB formation in secretory mutants and by Ca(2+) required the PB components Pat1 and Scd6, and calmodulin, indicating that different stressors act through distinct pathways...
  34. Dutko J, Kenny A, Gamache E, Curcio M. 5' to 3' mRNA decay factors colocalize with Ty1 gag and human APOBEC3G and promote Ty1 retrotransposition. J Virol. 2010;84:5052-66 pubmed publisher
    ..Together, the results suggest that VLPs assemble in P bodies and that 5' to 3' mRNA decay is essential for the packaging of Ty1 RNA in VLPs. ..
  35. Drummond S, Hildyard J, Firczuk H, Reamtong O, Li N, Kannambath S, et al. Diauxic shift-dependent relocalization of decapping activators Dhh1 and Pat1 to polysomal complexes. Nucleic Acids Res. 2011;39:7764-74 pubmed publisher
    Dhh1 and Pat1 in yeast are mRNA decapping activators/translational repressors thought to play key roles in the transition of mRNAs from translation to degradation...
  36. He F, Jacobson A. Control of mRNA decapping by positive and negative regulatory elements in the Dcp2 C-terminal domain. RNA. 2015;21:1633-47 pubmed publisher
    ..The positive elements recruit the specific decapping activators Edc3, Pat1, and Upf1 to form distinct decapping complexes and control the enzyme's substrate specificity and final activation...
  37. Chowdhury A, Kalurupalle S, Tharun S. Mutagenic Analysis of the C-Terminal Extension of Lsm1. PLoS ONE. 2016;11:e0158876 pubmed publisher
    ..Lsm7 proteins are highly conserved in eukaryotes and they form a hetero-octameric complex together with the protein Pat1. The Lsm1-7-Pat1 complex plays a key role in mRNA decapping and 3'-end protection and therefore is required for ..
  38. Bahassou Benamri R, Davin A, Gaillard J, Alonso B, Odorico M, Pible O, et al. Subcellular localization and interaction network of the mRNA decay activator Pat1 upon UV stress. Yeast. 2013;30:353-63 pubmed publisher
    ..Their relocation was assayed using quantitative nuclear relocation assay (QNR). We focused on Pat1, a component of the cytoplasmic foci called processing bodies (p-bodies), because it had the strongest response to ..
  39. Hausmann S, Zheng S, Costanzo M, Brost R, Garcin D, Boone C, et al. Genetic and biochemical analysis of yeast and human cap trimethylguanosine synthase: functional overlap of 2,2,7-trimethylguanosine caps, small nuclear ribonucleoprotein components, pre-mRNA splicing factors, and RNA decay pathways. J Biol Chem. 2008;283:31706-18 pubmed publisher
    ..analysis also highlighted synthetic interactions of Tgs1 with proteins implicated in RNA end processing and decay (Pat1, Lsm1, and Trf4) and regulation of polymerase II transcription (Rpn4, Spt3, Srb2, Soh1, Swr1, and Htz1)...
  40. Wu D, Muhlrad D, Bowler M, Jiang S, Liu Z, Parker R, et al. Lsm2 and Lsm3 bridge the interaction of the Lsm1-7 complex with Pat1 for decapping activation. Cell Res. 2014;24:233-46 pubmed publisher
    The evolutionarily conserved Lsm1-7-Pat1 complex is the most critical activator of mRNA decapping in eukaryotic cells and plays many roles in normal decay, AU-rich element-mediated decay, and miRNA silencing, yet how Pat1 interacts with ..
  41. Makino S, Mishima Y, Inoue K, Inada T. Roles of mRNA fate modulators Dhh1 and Pat1 in TNRC6-dependent gene silencing recapitulated in yeast. J Biol Chem. 2015;290:8331-47 pubmed publisher
    ..Furthermore, the mRNA fate modulators Dhh1 and Pat1 redundantly stimulate mRNA decay, but both factors are required for poly(A) tail-independent translation repression ..
  42. Luo X, Talarek N, De Virgilio C. Initiation of the yeast G0 program requires Igo1 and Igo2, which antagonize activation of decapping of specific nutrient-regulated mRNAs. RNA Biol. 2011;8:14-7 pubmed
    ..Here, we show that several factors including Ccr4, the Lsm-Pat1 complex, and Dhh1, which are implicated in mRNA decapping activation, participate in the decay of specific mRNAs ..
  43. Zhang B, Shi Q, Varia S, Xing S, Klett B, Cook L, et al. The Activity-Dependent Regulation of Protein Kinase Stability by the Localization to P-Bodies. Genetics. 2016;203:1191-202 pubmed publisher
    ..Altogether, this work illustrates how the presence within an RNP granule can alter the ultimate fate of the localized protein. ..
  44. Gimenez Barcons M, Alves Rodrigues I, Jungfleisch J, Van Wynsberghe P, Ahlquist P, Diez J. The cellular decapping activators LSm1, Pat1, and Dhh1 control the ratio of subgenomic to genomic Flock House virus RNAs. J Virol. 2013;87:6192-200 pubmed publisher
    ..RNA viruses, such as hepatitis C virus and brome mosaic virus, depends on host decapping activators LSm1-7, Pat1, and Dhh1 (J. Diez et al., Proc. Natl. Acad. Sci. U. S. A. 97:3913-3918, 2000; A. Mas et al., J. Virol...
  45. Swisher K, Parker R. Interactions between Upf1 and the decapping factors Edc3 and Pat1 in Saccharomyces cerevisiae. PLoS ONE. 2011;6:e26547 pubmed publisher
    ..Upf2 or Upf3 leads to the accumulation of not only Upf1 and Dcp2 in P-bodies, but also of the decapping-activators Pat1, Dhh1, and Lsm1...