Gene Symbol: PAP2
Description: non-canonical poly(A) polymerase PAP2
Alias: TRF4, non-canonical poly(A) polymerase PAP2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Castaño I, Heath Pagliuso S, Sadoff B, Fitzhugh D, Christman M. A novel family of TRF (DNA topoisomerase I-related function) genes required for proper nuclear segregation. Nucleic Acids Res. 1996;24:2404-10 pubmed
    We recently reported the identification of a gene, TRF4 (for DNA topoisomerase related function), in a screen for mutations that are synthetically lethal with mutations in DNA topoisomerase I (top1)...
  2. Gellon L, Carson D, Carson J, Demple B. Intrinsic 5'-deoxyribose-5-phosphate lyase activity in Saccharomyces cerevisiae Trf4 protein with a possible role in base excision DNA repair. DNA Repair (Amst). 2008;7:187-98 pubmed
    ..Computational analysis indicates that yeast Trf4 protein, with roles in sister chromatin cohesion and RNA quality control, is a new member of the X family of DNA ..
  3. Rougemaille M, Gudipati R, Olesen J, Thomsen R, Seraphin B, Libri D, et al. Dissecting mechanisms of nuclear mRNA surveillance in THO/sub2 complex mutants. EMBO J. 2007;26:2317-26 pubmed
  4. Dez C, Houseley J, Tollervey D. Surveillance of nuclear-restricted pre-ribosomes within a subnucleolar region of Saccharomyces cerevisiae. EMBO J. 2006;25:1534-46 pubmed
    ..Localization of pre-ribosomes to this focus was lost in sda1-2 strains lacking Trf4p or Rrp6p. We designate this nucleolar focus the No-body and propose that it represents a site of pre-ribosome surveillance. ..
  5. Kadaba S, Wang X, Anderson J. Nuclear RNA surveillance in Saccharomyces cerevisiae: Trf4p-dependent polyadenylation of nascent hypomethylated tRNA and an aberrant form of 5S rRNA. RNA. 2006;12:508-21 pubmed
    ..We conclude that an array of RNA polymerase III transcripts are targeted for Trf4p/ Trf5p-dependent polyadenylation and turnover to eliminate mutant and variant forms of normally stable RNAs. ..
  6. Copela L, Fernandez C, Sherrer R, Wolin S. Competition between the Rex1 exonuclease and the La protein affects both Trf4p-mediated RNA quality control and pre-tRNA maturation. RNA. 2008;14:1214-27 pubmed publisher
    ..Our experiments reveal that one consequence of Rex1p-dependent 3' trimming is the generation of aberrant RNAs that are targeted for decay by TRAMP. ..
  7. Luke B, Panza A, Redon S, Iglesias N, Li Z, Lingner J. The Rat1p 5' to 3' exonuclease degrades telomeric repeat-containing RNA and promotes telomere elongation in Saccharomyces cerevisiae. Mol Cell. 2008;32:465-77 pubmed publisher
    ..Thus, telomeric transcription combined with Rat1p-dependent TERRA degradation is important for regulating telomerase in yeast. Telomere transcription is conserved in different kingdoms of the eukaryotic domain. ..
  8. Saitoh S, Chabes A, McDonald W, Thelander L, Yates J, Russell P. Cid13 is a cytoplasmic poly(A) polymerase that regulates ribonucleotide reductase mRNA. Cell. 2002;109:563-73 pubmed
    Fission yeast Cid13 and budding yeast Trf4/5 are members of a newly identified nucleotidyltransferase family conserved from yeast to man. Trf4/5 are thought to be essential DNA polymerases. We report that Cid13 is a poly(A) polymerase...
  9. Sadoff B, Heath Pagliuso S, Castaño I, Zhu Y, Kieff F, Christman M. Isolation of mutants of Saccharomyces cerevisiae requiring DNA topoisomerase I. Genetics. 1995;141:465-79 pubmed
    ..TRF1 is allelic to HPR1, previously shown to be homologous to TOP1 over two short regions. TRF4 encodes a novel 584-amino acid protein with homology to the N-terminus of Saccharomyces cerevisiae topo I...

More Information


  1. Houseley J, Tollervey D. Yeast Trf5p is a nuclear poly(A) polymerase. EMBO Rep. 2006;7:205-11 pubmed
    ..Trf5p co-purified with Mtr4p and Air1p, indicating that it forms a complex, designated TRAMP5, that has functions that partially overlap with the TRAMP complex. ..
  2. Vasiljeva L, Buratowski S. Nrd1 interacts with the nuclear exosome for 3' processing of RNA polymerase II transcripts. Mol Cell. 2006;21:239-48 pubmed
    ..Since Nrd1 is known to bind RNA polymerase II and be important for sn/snoRNA 3' end processing, Nrd1 may link transcription and RNA 3' end formation with surveillance by the exosome. ..
  3. Hamill S, Wolin S, Reinisch K. Structure and function of the polymerase core of TRAMP, a RNA surveillance complex. Proc Natl Acad Sci U S A. 2010;107:15045-50 pubmed publisher
    ..Finally, we show that the RNA 3' end plays a role in substrate recognition. ..
  4. Houseley J, Kotovic K, El Hage A, Tollervey D. Trf4 targets ncRNAs from telomeric and rDNA spacer regions and functions in rDNA copy number control. EMBO J. 2007;26:4996-5006 pubmed
    b>Trf4 is the poly(A) polymerase component of TRAMP4, which stimulates nuclear RNA degradation by the exosome...
  5. Edwards S, Li C, Levy D, Brown J, Snow P, Campbell J. Saccharomyces cerevisiae DNA polymerase epsilon and polymerase sigma interact physically and functionally, suggesting a role for polymerase epsilon in sister chromatid cohesion. Mol Cell Biol. 2003;23:2733-48 pubmed
    ..sigma (Pol sigma), a recently identified, essential nuclear nucleotidyl transferase encoded by two redundant genes, TRF4 and TRF5...
  6. El Hage A, French S, Beyer A, Tollervey D. Loss of Topoisomerase I leads to R-loop-mediated transcriptional blocks during ribosomal RNA synthesis. Genes Dev. 2010;24:1546-58 pubmed publisher
    ..Topoisomerase I (Top1) generated truncated pre-rRNA fragments, which were stabilized in strains lacking TRAMP (Trf4/Trf5-Air1/Air2-Mtr4 polyadenylation complexes) or exosome degradation activities...
  7. Fasken M, Leung S, Banerjee A, Kodani M, Chavez R, Bowman E, et al. Air1 zinc knuckles 4 and 5 and a conserved IWRXY motif are critical for the function and integrity of the Trf4/5-Air1/2-Mtr4 polyadenylation (TRAMP) RNA quality control complex. J Biol Chem. 2011;286:37429-45 pubmed publisher
    ..The Trf4/5-Air1/2-Mtr4 polyadenylation (TRAMP) complex in S...
  8. Vanacova S, Wolf J, Martin G, Blank D, Dettwiler S, Friedlein A, et al. A new yeast poly(A) polymerase complex involved in RNA quality control. PLoS Biol. 2005;3:e189 pubmed
    ..unmodified counterpart revealed that the unmodified RNA was preferentially polyadenylated by affinity-purified Trf4 complex from yeast, as well as by complexes reconstituted from recombinant components...
  9. San Paolo S, Vanacova S, Schenk L, Scherrer T, Blank D, Keller W, et al. Distinct roles of non-canonical poly(A) polymerases in RNA metabolism. PLoS Genet. 2009;5:e1000555 pubmed publisher
    ..To assess the level of functional redundancy between the paralogous Trf4 and Trf5 proteins and to investigate the role of the Trf4-dependent polyadenylation in vivo, we used DNA ..
  10. Wang Z, Castaño I, Adams C, Vu C, Fitzhugh D, Christman M. Structure/function analysis of the Saccharomyces cerevisiae Trf4/Pol sigma DNA polymerase. Genetics. 2002;160:381-91 pubmed
    ..The polymerase is encoded by two redundant homologs in Saccharomyces cerevisiae, TRF4 and TRF5, that together define a fourth essential nuclear DNA polymerase in yeast and probably in all eukaryotes...
  11. Camblong J, Iglesias N, Fickentscher C, Dieppois G, Stutz F. Antisense RNA stabilization induces transcriptional gene silencing via histone deacetylation in S. cerevisiae. Cell. 2007;131:706-17 pubmed
  12. Arigo J, Eyler D, Carroll K, Corden J. Termination of cryptic unstable transcripts is directed by yeast RNA-binding proteins Nrd1 and Nab3. Mol Cell. 2006;23:841-51 pubmed
    ..These results suggest that transcription termination of CUTs directed by Nrd1 and Nab3 is a prerequisite for rapid degradation by the nuclear exosome. ..
  13. Callahan K, Butler J. TRAMP complex enhances RNA degradation by the nuclear exosome component Rrp6. J Biol Chem. 2010;285:3540-7 pubmed publisher
    ..for the enhancement of Rrp6 activity by TRAMP, suggesting that neither the poly(A) polymerase activity of Trf4 nor the helicase activity of Mtr4 plays a role in the enhancement...
  14. Schneider C, Anderson J, Tollervey D. The exosome subunit Rrp44 plays a direct role in RNA substrate recognition. Mol Cell. 2007;27:324-31 pubmed
    ..Recognition of hypomodified tRNA(i)(Met) by Rrp44 is genetically separable from its catalytic activity on other substrates, with the mutations mapping to distinct regions of the protein. ..
  15. Kadaba S, Krueger A, Trice T, Krecic A, Hinnebusch A, Anderson J. Nuclear surveillance and degradation of hypomodified initiator tRNAMet in S. cerevisiae. Genes Dev. 2004;18:1227-40 pubmed
    ..Whereas deletion of TRF4 leads to stabilization of tRNA(i)(Met), overexpression of Trf4p destabilizes the hypomodified tRNA(i)(Met) in trm6 ..
  16. Wyers F, Rougemaille M, Badis G, Rousselle J, Dufour M, Boulay J, et al. Cryptic pol II transcripts are degraded by a nuclear quality control pathway involving a new poly(A) polymerase. Cell. 2005;121:725-37 pubmed
    ..rapidly degraded by the combined action of the exosome and a new poly(A) polymerase activity that is defined by the Trf4 protein and one of two RNA binding proteins, Air1p or Air2p...
  17. Heo D, Yoo I, Kong J, Lidschreiber M, Mayer A, Choi B, et al. The RNA polymerase II C-terminal domain-interacting domain of yeast Nrd1 contributes to the choice of termination pathway and couples to RNA processing by the nuclear exosome. J Biol Chem. 2013;288:36676-90 pubmed publisher
    ..Surprisingly, replacing the Nrd1 CID with that from Rtt103 reduces binding to Rrp6/Trf4, and RNA transcripts terminated by Nrd1(CID(Rtt103)) are predominantly processed by core exosome...
  18. Wang Z, Castaño I, De Las Penas A, Adams C, Christman M. Pol kappa: A DNA polymerase required for sister chromatid cohesion. Science. 2000;289:774-9 pubmed
    ..Here we report that TRF4, an evolutionarily conserved gene necessary for chromosome segregation, encodes a DNA polymerase with beta-..
  19. Weir J, Bonneau F, Hentschel J, Conti E. Structural analysis reveals the characteristic features of Mtr4, a DExH helicase involved in nuclear RNA processing and surveillance. Proc Natl Acad Sci U S A. 2010;107:12139-44 pubmed publisher
    ..8S ribosomal RNA (rRNA). It also interacts with the polyadenylating Trf4-Air2 heterodimer to form the so-called TRAMP (Trf4-Air2-Mtr4 Polyadenylation) complex...
  20. Holub P, Lalakova J, Cerná H, Pasulka J, Sarazova M, Hrazdilova K, et al. Air2p is critical for the assembly and RNA-binding of the TRAMP complex and the KOW domain of Mtr4p is crucial for exosome activation. Nucleic Acids Res. 2012;40:5679-93 pubmed publisher
    b>Trf4/5p-Air1/2p-Mtr4p polyadenylation complex (TRAMP) is an essential component of nuclear RNA surveillance in yeast...
  21. LaCava J, Houseley J, Saveanu C, Petfalski E, Thompson E, Jacquier A, et al. RNA degradation by the exosome is promoted by a nuclear polyadenylation complex. Cell. 2005;121:713-24 pubmed
    ..We speculate that this function was maintained in eukaryotic nuclei, while cytoplasmic mRNA poly(A) tails acquired different roles in translation. ..
  22. Tudek A, Porrúa O, Kabzinski T, Lidschreiber M, Kubicek K, Fortova A, et al. Molecular basis for coordinating transcription termination with noncoding RNA degradation. Mol Cell. 2014;55:467-81 pubmed publisher
    ..The Trf4-Air2-Mtr4 (TRAMP) complex polyadenylates NNS target RNAs and favors their degradation...
  23. Reis C, Campbell J. Contribution of Trf4/5 and the nuclear exosome to genome stability through regulation of histone mRNA levels in Saccharomyces cerevisiae. Genetics. 2007;175:993-1010 pubmed
    ..The Saccharomyces cerevisiae poly(A) polymerases Trf4 and Trf5 are involved in a quality control mechanism that mediates polyadenylation and consequent degradation of ..
  24. Egecioglu D, Henras A, Chanfreau G. Contributions of Trf4p- and Trf5p-dependent polyadenylation to the processing and degradative functions of the yeast nuclear exosome. RNA. 2006;12:26-32 pubmed
    ..These results suggest that polyadenylation of RNA processing intermediates plays a functional role in RNA processing pathways and is not limited to RNA surveillance functions. ..
  25. Grzechnik P, Kufel J. Polyadenylation linked to transcription termination directs the processing of snoRNA precursors in yeast. Mol Cell. 2008;32:247-58 pubmed publisher
    ..A more important role of Trf4/TRAMP, however, is to enhance Nrd1 association with snoRNA genes...
  26. Berretta J, Pinskaya M, Morillon A. A cryptic unstable transcript mediates transcriptional trans-silencing of the Ty1 retrotransposon in S. cerevisiae. Genes Dev. 2008;22:615-26 pubmed publisher
    ..Our results show the first example of an RNA-dependent gene trans-silencing mediated by epigenetic marks in S. cerevisiae. ..
  27. Castaño I, Brzoska P, Sadoff B, Chen H, Christman M. Mitotic chromosome condensation in the rDNA requires TRF4 and DNA topoisomerase I in Saccharomyces cerevisiae. Genes Dev. 1996;10:2564-76 pubmed
    ..The TRF4 gene is also nonessential and was identified in a screen for mutations that are inviable in combination with a top1 ..
  28. Jia H, Wang X, Anderson J, Jankowsky E. RNA unwinding by the Trf4/Air2/Mtr4 polyadenylation (TRAMP) complex. Proc Natl Acad Sci U S A. 2012;109:7292-7 pubmed publisher
    Many RNA-processing events in the cell nucleus involve the Trf4/Air2/Mtr4 polyadenylation (TRAMP) complex, which contains the poly(A) polymerase Trf4p, the Zn-knuckle protein Air2p, and the RNA helicase Mtr4p...
  29. Azzouz N, Panasenko O, Colau G, Collart M. The CCR4-NOT complex physically and functionally interacts with TRAMP and the nuclear exosome. PLoS ONE. 2009;4:e6760 pubmed publisher
    ..Our findings connect for the first time the different players involved in nuclear and cytoplasmic RNA degradation. ..
  30. Walowsky C, Fitzhugh D, Castaño I, Ju J, Levin N, Christman M. The topoisomerase-related function gene TRF4 affects cellular sensitivity to the antitumor agent camptothecin. J Biol Chem. 1999;274:7302-8 pubmed
    ..We report here that two genes required for sister chromatid cohesion, TRF4 and MCD1/SCC1, are also required to repair camptothecin-mediated damage to DNA...
  31. Wahba L, Amon J, Koshland D, Vuica Ross M. RNase H and multiple RNA biogenesis factors cooperate to prevent RNA:DNA hybrids from generating genome instability. Mol Cell. 2011;44:978-88 pubmed publisher
    ..In summary, RNA:DNA hybrids are a potent source for changing genome structure. By preventing their formation and accumulation, multiple RNA biogenesis factors and RNase H act as guardians of the genome. ..
  32. Frenk S, Oxley D, Houseley J. The nuclear exosome is active and important during budding yeast meiosis. PLoS ONE. 2014;9:e107648 pubmed publisher
    ..We have analysed the activity of the nuclear exosome during meiosis by deletion of TRF4, which encodes a key component of the exosome targeting complex TRAMP...
  33. Lai X, Beilharz T, Au W, Hammet A, Preiss T, Basrai M, et al. Yeast hEST1A/B (SMG5/6)-like proteins contribute to environment-sensing adaptive gene expression responses. G3 (Bethesda). 2013;3:1649-59 pubmed publisher
    ..Overall, these results suggest that Esl1 and Esl2 contribute to the regulation of adaptive gene expression responses of environmental sensing pathways. ..
  34. Durand Dubief M, Will W, Petrini E, Theodorou D, Harris R, Crawford M, et al. SWI/SNF-like chromatin remodeling factor Fun30 supports point centromere function in S. cerevisiae. PLoS Genet. 2012;8:e1002974 pubmed publisher
    ..As centromeres in budding yeast are not embedded in heterochromatin, our findings indicate a direct role of Fun30 in centromere chromatin by promoting correct chromatin architecture. ..
  35. Gavald S, Gallardo M, Luna R, Aguilera A. R-loop mediated transcription-associated recombination in trf4? mutants reveals new links between RNA surveillance and genome integrity. PLoS ONE. 2013;8:e65541 pubmed publisher
    ..We have identified trf4, a gene encoding a non-canonical polyA-polymerase involved in RNA surveillance, as a factor that prevents ..
  36. Hausmann S, Zheng S, Costanzo M, Brost R, Garcin D, Boone C, et al. Genetic and biochemical analysis of yeast and human cap trimethylguanosine synthase: functional overlap of 2,2,7-trimethylguanosine caps, small nuclear ribonucleoprotein components, pre-mRNA splicing factors, and RNA decay pathways. J Biol Chem. 2008;283:31706-18 pubmed publisher
    ..synthetic interactions of Tgs1 with proteins implicated in RNA end processing and decay (Pat1, Lsm1, and Trf4) and regulation of polymerase II transcription (Rpn4, Spt3, Srb2, Soh1, Swr1, and Htz1)...
  37. Chernyakov I, Whipple J, Kotelawala L, Grayhack E, Phizicky E. Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1. Genes Dev. 2008;22:1369-80 pubmed publisher
    ..The RTD pathway is the first to be implicated in the turnover of mature RNA species from the class of stable RNAs. These results and the results of others demonstrate that tRNA, like mRNA, is subject to multiple quality control steps...
  38. Barnard E, McFerran N, Trudgett A, Nelson J, Timson D. Detection and localisation of protein-protein interactions in Saccharomyces cerevisiae using a split-GFP method. Fungal Genet Biol. 2008;45:597-604 pubmed publisher
  39. Dickinson H, Tretbar S, Betat H, Mörl M. The TRAMP complex shows tRNA editing activity in S. cerevisiae. Mol Biol Evol. 2012;29:1451-9 pubmed publisher
    ..Accordingly, our observations are a strong experimental support for the hypothesis that enzymatic promiscuity serves as an evolutionary starting point for the emergence of new functions and activities. ..
  40. Clemente Blanco A, Sen N, Mayán Santos M, Sacristán M, Graham B, Jarmuz A, et al. Cdc14 phosphatase promotes segregation of telomeres through repression of RNA polymerase II transcription. Nat Cell Biol. 2011;13:1450-6 pubmed publisher
    ..Finally, telomere segregation defects in cdc14 mutants(4) correlate with the presence of subtelomeric Y' elements and can be rescued by transcriptional inhibition of RNA polymerase II. ..
  41. Fasken M, Laribee R, Corbett A. Nab3 facilitates the function of the TRAMP complex in RNA processing via recruitment of Rrp6 independent of Nrd1. PLoS Genet. 2015;11:e1005044 pubmed publisher
    ..The TRAMP (Trf4/5-Air1/2-Mtr4 polyadenylation) complex is a critical exosome cofactor in budding yeast that stimulates the exosome ..
  42. Schmid M, Poulsen M, Olszewski P, Pelechano V, Saguez C, Gupta I, et al. Rrp6p controls mRNA poly(A) tail length and its decoration with poly(A) binding proteins. Mol Cell. 2012;47:267-80 pubmed publisher
    ..We suggest that a nuclear mRNP surveillance step involves targeting of Rrp6p by Nab2p-bound pA-tailed RNPs and that pre-mRNA abundance is regulated at this level. ..
  43. Wang X, Jia H, Jankowsky E, Anderson J. Degradation of hypomodified tRNA(iMet) in vivo involves RNA-dependent ATPase activity of the DExH helicase Mtr4p. RNA. 2008;14:107-16 pubmed
    ..Unwinding and RNA-stimulated ATPase activities are strongly reduced in the recombinant mutant Mtr4-20p, suggesting that these activities of Mtr4p are critical for degradation of polyadenylated hypomodified tRNA(iMet). ..
  44. Kim K, Heo D, Kim I, Suh J, Kim M. Exosome Cofactors Connect Transcription Termination to RNA Processing by Guiding Terminated Transcripts to the Appropriate Exonuclease within the Nuclear Exosome. J Biol Chem. 2016;291:13229-42 pubmed publisher
    ..Here, we show that two nuclear exosome cofactors, Mpp6 and Trf4, directly and competitively interact with the Nrd1 CID and differentially regulate the association of Nrd1 with two ..
  45. Losh J, King A, Bakelar J, Taylor L, Loomis J, Rosenzweig J, et al. Interaction between the RNA-dependent ATPase and poly(A) polymerase subunits of the TRAMP complex is mediated by short peptides and important for snoRNA processing. Nucleic Acids Res. 2015;43:1848-58 pubmed
    ..nuclear RNA-dependent ATPase Mtr4, its cytoplasmic paralog Ski2 and the nuclear non-canonical poly(A) polymerases, Trf4 and Trf5. Mtr4 and either Trf4 or Trf5 assemble into a TRAMP complex...
  46. Falk S, Weir J, Hentschel J, Reichelt P, Bonneau F, Conti E. The molecular architecture of the TRAMP complex reveals the organization and interplay of its two catalytic activities. Mol Cell. 2014;55:856-867 pubmed publisher
    ..TRAMP is associated with the nuclear exosome and consists of a poly(A)polymerase subcomplex (Trf4-Air2) and a helicase (Mtr4)...
  47. Leung E, Schneider C, Yan F, Mohi El Din H, Kudla G, Tuck A, et al. Integrity of SRP RNA is ensured by La and the nuclear RNA quality control machinery. Nucleic Acids Res. 2014;42:10698-710 pubmed publisher
    ..We conclude that the RNA surveillance machinery has key roles in both SRP biogenesis and quality control of the RNA, potentially facilitating the decision between these alternative fates. ..
  48. Kong K, Tang H, Pan K, Huang Z, Lee T, Hinnebusch A, et al. Cotranscriptional recruitment of yeast TRAMP complex to intronic sequences promotes optimal pre-mRNA splicing. Nucleic Acids Res. 2014;42:643-60 pubmed publisher
    ..these unwanted RNA transcripts, including spliced-out introns, are first recognized by the nuclear exosome cofactor Trf4/5p-Air1/2p-Mtr4p polyadenylation (TRAMP) complex before subsequent nuclear-exosome-mediated degradation...
  49. Granato D, Machado Santelli G, Oliveira C. Nop53p interacts with 5.8S rRNA co-transcriptionally, and regulates processing of pre-rRNA by the exosome. FEBS J. 2008;275:4164-78 pubmed publisher
    ..Consistent with this observation and similar to the observed in exosome mutants, depletion of Nop53p leads to accumulation of polyadenylated pre-rRNAs. ..
  50. Thomson E, Tollervey D. The final step in 5.8S rRNA processing is cytoplasmic in Saccharomyces cerevisiae. Mol Cell Biol. 2010;30:976-84 pubmed publisher
    ..We conclude that nuclear pre-60S particles containing the 6S pre-rRNA bind Nmd3 and Crm1 and are exported to the cytoplasm prior to final maturation by Ngl2. ..
  51. Alexandrov A, Chernyakov I, Gu W, Hiley S, Hughes T, Grayhack E, et al. Rapid tRNA decay can result from lack of nonessential modifications. Mol Cell. 2006;21:87-96 pubmed
    ..These results define a rapid tRNA degradation (RTD) pathway that is independent of the TRF4/RRP6-dependent nuclear surveillance pathway...
  52. Salvi J, Chan J, Szafranski K, Liu T, Wu J, Olsen J, et al. Roles for Pbp1 and caloric restriction in genome and lifespan maintenance via suppression of RNA-DNA hybrids. Dev Cell. 2014;30:177-91 pubmed publisher
    ..Thus, we find roles for Pbp1 in genome maintenance and reveal that caloric restriction counteracts Pbp1 deficiencies by engaging RNaseH and Pif1...
  53. Stuparevic I, Mosrin Huaman C, Hervouet Coste N, Remenaric M, Rahmouni A. Cotranscriptional recruitment of RNA exosome cofactors Rrp47p and Mpp6p and two distinct Trf-Air-Mtr4 polyadenylation (TRAMP) complexes assists the exonuclease Rrp6p in the targeting and degradation of an aberrant messenger ribonucleoprotein particle. J Biol Chem. 2013;288:31816-29 pubmed publisher
    ..Together, our results provide an integrated view of how different cofactors of the RNA degradation machinery cooperate to target and eliminate aberrant mRNPs...
  54. Haracska L, Johnson R, Prakash L, Prakash S. Trf4 and Trf5 proteins of Saccharomyces cerevisiae exhibit poly(A) RNA polymerase activity but no DNA polymerase activity. Mol Cell Biol. 2005;25:10183-9 pubmed
    The Saccharomyces cerevisiae Trf4 and Trf5 proteins are members of a distinct family of eukaryotic DNA polymerase beta-like nucleotidyltransferases, and a template-dependent DNA polymerase activity has been reported for Trf4...
  55. Roth K, Byam J, Fang F, Butler J. Regulation of NAB2 mRNA 3'-end formation requires the core exosome and the Trf4p component of the TRAMP complex. RNA. 2009;15:1045-58 pubmed publisher
    ..These findings suggest that NAB2 mRNA 3'-end formation requires the exosome and TRAMP complex, and that competition between polyadenylation and Rrp6p-dependent degradation controls the level of this mRNA. ..